Article
Developmental Biology
Olga G. Jaime, Jessica Arias, Shreya Pavani, April D. Pyle, Michael R. Hicks
Summary: The co-expression of SIX1 and PAX3 is the most indicative factor for skeletal muscle differentiation in human pluripotent stem cells (hPSCs). Manipulating seeding density, monitoring metabolic secretion, and altering the concentration of CHIR99021 can enhance the emergence of SIX1+PAX3+ precursors in hPSC differentiation. Inhibition of non-myogenic lineages can modulate PAX3 expression independently of SIX1.
Article
Engineering, Biomedical
Hanhao Dai, Jun Luo, Lili Deng, Chao Song, Zhibo Deng, Yijing Wu, Song Gu, Jie Xu
Summary: Sarcopenia is a geriatric disease characterized by reduced muscle function and mass. It is identified that miR-467a-3p and miR-874-5p can respectively mediate the stemness and myogenesis of satellite cells (SCs) by performing bioinformatics analysis. EVs loaded with ant-467a and ant-874 are more effective than their naked counterparts in promoting the stemness and myogenesis of SCs.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Hyunjung Lee, Young In Kim, Farida S. Nirmala, Ji-Sun Kim, Hyo-Deok Seo, Tae Youl Ha, Young-Jin Jang, Chang Hwa Jung, Jiyun Ahn
Summary: Post-menopausal conditions exacerbate the biological aging process, leading to visceral adiposity and sarcopenia. Mitochondrial impairment, along with the upregulation of miR-141-3p and subsequent downregulation of Fkbp5 and Fibin, plays a role in the development of obesogenic sarcopenia. Inhibiting miR-141-3p could be a potential therapeutic target for alleviating this condition.
Article
Biotechnology & Applied Microbiology
Jun'an Shi, Wendong Li, Anfang Liu, Lingtong Ren, Pusen Zhang, Ting Jiang, Yuqing Han, Lingbin Liu
Summary: This study identified miRNAs and genes related to muscle development by extracting RNA from the leg muscles of Chengkou Mountain Chicken embryos. Differential analysis and weighted correlation network analysis were conducted to determine key miRNAs and modules at different developmental stages. These findings lay the foundation for further research on the molecular regulatory mechanism of embryonic muscle development in Chengkou Mountain Chicken and provide reference for muscle development studies in other poultry and livestock.
Review
Biochemistry & Molecular Biology
Emmanuel Odame, Yuan Chen, Shuailong Zheng, Dinghui Dai, Bismark Kyei, Siyuan Zhan, Jiaxue Cao, Jiazhong Guo, Tao Zhong, Linjie Wang, Li Li, Hongping Zhang
Summary: miRNAs are well-known gene repressors, while a newly identified class named NamiRNAs function as gene activators by binding to enhancer features. The activated enhancers produce eRNAs to enhance gene expression, interacting with NamiRNAs to perform similar functions. This review explores the functional differences and similarities between eRNAs and NamiRNAs in myogenesis and diseases.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Review
Biochemistry & Molecular Biology
Amelia Eva Aranega, Estefania Lozano-Velasco, Lara Rodriguez-Outeirino, Felicitas Ramirez de Acuna, Diego Franco, Francisco Hernandez-Torres
Summary: miRNAs play a critical role in modulating muscle regeneration and stem cell behavior, which is essential for treating muscle disorders. Recent advancements suggest that optimizing muscle stem cell response through miRNAs, in conjunction with gene replacement therapies, can improve muscle regeneration in the context of DMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Li Wan, Ru-Meng Jia, Lu-Lu Ji, Xin-Miao Qin, Liang Hu, Fan Hu, Yuan Han, Yin-Bing Pan, Chun-Yi Jiang, Wen-Tao Liu
Summary: This study found that AMPK-autophagy activation can suppress neuroinflammation and improve morphine tolerance by upregulating SOCS3 through the inhibition of miRNA-30a-5p.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Biochemistry & Molecular Biology
Ombline Conrad, Mickael Burgy, Sophie Foppolo, Aude Jehl, Alicia Thiery, Sebastien Guihard, Romain Vauchelles, Alain C. C. Jung, Jana Mourtada, Christine Macabre, Sonia Ledrappier, Marie-Pierre Chenard, Mihaela-Alina Onea, Aurelien Danic, Thomas Dourlhes, Claire Thibault, Philippe Schultz, Monique Dontenwill, Sophie Martin
Summary: The expression levels of the 3p passenger strands of miR-30a and miR-30e are correlated with the behavior and prognosis of head and neck squamous cell carcinoma. Low expression of miR-30a and miR-30e is associated with adverse prognosis and can potentially be used as biomarkers for diagnosis and treatment of head and neck squamous cell carcinoma. Additionally, miR-30a and miR-30e expression can affect tumor cell behavior and the tumor microenvironment, potentially improving treatment outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Arundhati Das, Sharmishtha Shyamal, Tanvi Sinha, Smruti Sambhav Mishra, Amaresh C. Panda
Summary: Circular RNAs are a newly discovered family of regulatory RNAs that are generated through backsplicing and regulate gene expression by acting as a sponge for RNA-binding proteins and microRNAs. The study found abundant circRNAs in mouse skeletal muscle, aging human skeletal muscle tissue, and satellite cells, with some potentially regulating myogenesis through the miR-204-5p/Mef2c signaling pathway.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Endocrinology & Metabolism
Peng Wang, Rong Pan, John Weaver, Mengjie Jia, Xue Yang, Tianhui Yang, Jia Liang, Ke J. Liu
Summary: The study revealed that miR-30a plays a role in mediating BBB damage after ischemic stroke and its inhibitor can reduce zinc accumulation, increase ZnT4 expression, and prevent BBB damage. miR-30a could be a potential therapeutic target for ischemic stroke.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2021)
Article
Anatomy & Morphology
Hanna Nord, Abraha Kahsay, Nils Dennhag, Fatima Pedrosa Domellof, Jonas von Hofsten
Summary: The study revealed that Pax3 and Pax7 are redundantly required for appendicular myogenesis in zebrafish, with Pax7 capable of activating the same developmental programs as Pax3 in premigratory progenitor cells.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Xinyong Cai, Ping Zhang, Yong Yang, Yunxia Wang, Hongmin Zhu, Bin Li, Hong Zeng, Lang Hong, Liang Shao
Summary: The study aimed to investigate the role of miR-30a-5p in restenosis following vein grafting in rats and its underlying mechanism. The results showed that miR-30a-5p expression gradually increased, ATG5 expression gradually decreased, and the intima thickened during restenosis. It was concluded that miR-30a-5p exacerbates vein graft restenosis by repressing ATG5 expression and inhibiting autophagy.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Immunology
Xinxin Liu, Wenqiang Tang, Kemin Chen, Yuxia Wang, Jie Qin, Fengrui Yang
Summary: This study reveals the cardioprotective role of circ_0002612 against myocardial ischemia/reperfusion injury (MI/RI), and identifies its mechanistic actions. circ_0002612 competitively binds to miR-30a-5p to release the expression of its target gene Ppargc1a, promoting cardiomyocyte viability and suppressing apoptosis. Furthermore, circ_0002612 inhibits the expression of NLRP3, leading to enhanced cardiomyocyte proliferation and reduced apoptosis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tomas J. Steeman, Andrea M. J. Weiner, Aldana P. David, Andres Binolfi, Nora B. Calcaterra, Pablo Armas
Summary: RNA guanine quadruplexes (G4s) formed within precursors of microRNAs (pre-miRNAs) regulate miRNA biogenesis during zebrafish embryogenesis. The G4 formed in the precursor of miRNA 150 (pre-miR-150) acts as a conserved regulatory structure competing with the stem-loop structure necessary for miRNA biogenesis, leading to knock-down phenotypes associated with the target gene myb. Inhibition of G4 formation and stabilization using a G4 ligand rescues the phenotypes related to myb knock-down.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Mervat Naguib, Mohamed Magdy, Omar Ahmed Elsayed Yousef, Walaa Ibrahim, Doaa Mostafa Gharib
Summary: In women with obesity, microRNA-30a, beclin1, age, and ALT and TSH levels were significantly associated with the metabolically unhealthy obesity phenotype, with microRNA-30a being the best indicator of metabolic syndrome.
DIABETES METABOLIC SYNDROME AND OBESITY
(2023)
Editorial Material
Developmental Biology
Kristin Bruk Artinger, Dominique Alfandari, Lisa A. Taneyhill
MECHANISMS OF DEVELOPMENT
(2017)
Article
Developmental Biology
Jason S. Williams, Jessica Y. Hsu, Christy Cortez Rossi, Kristin Bruk Artinger
DEVELOPMENTAL BIOLOGY
(2018)
Article
Developmental Biology
Lomeli Carpio Shull, Rwik Sen, Johannes Menzel, Susumu Goyama, Mineo Kurokawa, Kristin Bruk Artinger
DEVELOPMENTAL BIOLOGY
(2020)
Article
Developmental Biology
Sofia A. Pezoa, Kristin B. Artinger, Lee A. Niswander
DEVELOPMENTAL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Ritsuko Iwanaga, Brittany T. Truong, Jessica Y. Hsu, Karoline A. Lambert, Rajesh Vyas, David Orlicky, Yiqun G. Shellman, Aik-Choon Tan, Craig Ceol, Kristin Bruk Artinger
MOLECULAR CARCINOGENESIS
(2020)
Editorial Material
Developmental Biology
Kristin Bruk Artinger
BIRTH DEFECTS RESEARCH
(2020)
Review
Developmental Biology
Brittany T. Truong, Kristin B. Artinger
Summary: Even though craniofacial and limb defects appear to have distinct gene regulatory networks, they often co-occur, suggesting commonalities between these two types of congenital anomalies. Using zebrafish models, studies can delve into the mechanisms of human syndromes to gain a deeper understanding of the underlying causes of these deformities.
Article
Biology
Ezra Lencer, Rytis Prekeris, Kristin Bruk Artinger
Summary: This study utilized single-cell RNA sequencing to characterize transcriptional changes associated with neural crest cell development in the zebrafish trunk during the early stages of migration. The findings revealed transcriptional diversity in neural crest cells, early expression of genes associated with differentiated derivatives in pre-migratory stages, and the identification of a population of Rohon-Beard neurons. The data presented provide novel genetic markers for multiple trunk neural crest cell populations and Rohon-Beard neurons, shedding light on previously uncharacterized genes critical for vertebrate development.
Article
Developmental Biology
Lomeli C. Shull, Ezra S. Lencer, Hyun Min Kim, Susumu Goyama, Mineo Kurokawa, James C. Costello, Kenneth Jones, Kristin B. Artinger
Summary: This study demonstrates that transcription factors and histone methyltransferase proteins Prdm3 and Prdm16 play crucial roles in the differentiation switch of cranial neural crest cells (NCCs) to craniofacial cartilage. The loss of either protein leads to abnormal chondrocyte development, which is regulated by controlling the timing of Wnt/beta-catenin activity and chromatin accessibility. Manipulating Wnt/beta-catenin signaling or generating double mutants of prdm3(-/-) and prdm16(-/-) can rescue craniofacial cartilage defects.
Article
Cell Biology
Jessica Y. Hsu, Etienne P. Danis, Stephanie Nance, Jenean H. O'Brien, Annika L. Gustafson, Veronica M. Wessells, Andrew E. Goodspeed, Jared C. Talbot, Sharon L. Amacher, Paul Jedlicka, Joshua C. Black, James C. Costello, Adam D. Durbin, Kristin B. Artinger, Heide L. Ford
Summary: This study demonstrates the crucial role of SIX1 in maintaining the undifferentiated state of rhabdomyosarcoma (RMS) and inhibiting tumor growth. SIX1 controls enhancer activity and MYOD1 occupancy at specific loci to preserve the undifferentiated state of RMS cells.
Letter
Developmental Biology
Kristin B. Artinger, Michiko Watanabe
BIRTH DEFECTS RESEARCH
(2022)
Editorial Material
Developmental Biology
Poorni Iyer, Michiko Watanabe, Kristin B. Artinger
BIRTH DEFECTS RESEARCH
(2023)
Article
Developmental Biology
Ezra Lencer, Addison Rains, Erin Binne, Rytis Prekeris, Kristin B. Artinger
Summary: The transmembrane proteins cdon and boc are involved in regulating hedgehog signaling and have additional roles in axon guidance and neural crest cell migration. The study investigates the function of cdon and boc in zebrafish neural crest cell migration using mutants and finds disruption of migration in double cdon;boc mutants. The study also reveals defects in slow-twitch muscle cell differentiation and extracellular matrix loss, suggesting that neural crest defects may be secondary to mesoderm development defects.
Review
Developmental Biology
Lomeli C. Shull, Kristin B. Artinger
Summary: In this review, the role of epigenetic factors during neural crest development, specifically in craniofacial development, is discussed, as well as how compromised activities of these regulators contribute to congenital defects affecting the craniofacial complex.
BIRTH DEFECTS RESEARCH
(2023)
Article
Developmental Biology
Rwik Sen, Sofia A. Pezoa, Lomeli Carpio Shull, Laura Hernandez-Lagunas, Lee A. Niswander, Kristin Bruk Artinger
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2018)