Article
Nanoscience & Nanotechnology
Chikahiro Imashiro, Byungjun Kang, Yunam Lee, Youn-Hoo Hwang, Seonghun Im, Dae-Eun Kim, Kenjiro Takemura, Hyungsuk Lee
Summary: Collective cell migration is critical in various physiological and pathological processes, and this study investigated the effect of propagating acoustic waves on such migration behavior. The research found that cell migration was enhanced at a critical wave intensity but suppressed at higher intensities. The observed increased migration was potentially due to cell orientation alignment and thicker actin bundles indicative of higher traction force in response to the acoustic stimulation.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Chao Xie, Yuxiang Jiang, Zhiwen Zhu, Shanjin Huang, Wei Li, Guangshuo Ou
Summary: The evolutionarily conserved coronin family protein POD-1 debranches Arp2/3-nucleated actin filaments in migrating neuroblasts and asymmetrically dividing embryos, contributing to cell polarity and migration. Fluorescence live imaging analysis revealed POD-1 colocalization with Arp2/3 at the leading edge of neuroblasts, indicating their synergistic role in organizing actin networks and cell polarity. Conditional mutations of POD-1 disrupted actin assembly, cell polarity, and migration, highlighting the importance of F-actin debranching in regulating these cellular processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Carsten Baltes, Divyendu Goud Thalla, Uli Kazmaier, Franziska Lautenschlaeger
Summary: Actin plays a crucial role in maintaining cell shape and generating forces. By stabilizing actin filaments with miuraenamide A, we observed a decrease in filament dynamics and an elongation of filament length, resulting in altered cell migration speed and nuclear position.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Purnati Khuntia, Simran Rawal, Rituraj Marwaha, Tamal Das
Summary: The Golgi apparatus moves from an apical position to a basal position during epithelial cell migration, driven by actin cytoskeleton instead of microtubules. The interaction between Golgi and actin is mediated by actin projections, which is critical for cell migration.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jianjiang Hu, Xiaowei Gong, Staffan Stromblad
Summary: Cells probe their microenvironment using membrane protrusion-retraction cycles. Spatial and temporal coordination between Rac1 and RhoA GTP-binding activities play critical roles in initiating and reinforcing protrusions and retractions. Rac1-GTP levels increase before protrusions, while RhoA-GTP levels increase before retractions. Rac1-GTP nadirs and peaks coincide with maximal edge velocity of local membrane protrusions and retractions, followed by declined edge velocity. The alteration of local Rac1-GTP precedes the alteration of traction force. Through optogenetic Rac1-GTP perturbations, we demonstrate the fundamental role of Rac1 in restricting the size and durability of protrusions and retractions, possibly through controlling traction forces.
Review
Biochemistry & Molecular Biology
Nayden G. Naydenov, Jennifer E. Koblinski, Andrei I. Ivanov
Summary: The scaffolding protein Anillin interacts with major cytoskeletal structures and plays a key role in regulating cell division and other important activities in non-dividing cells. Its overexpression is associated with poor prognosis in solid cancers, and it is implicated in the regulation of tumor growth and metastasis through both canonical and novel functions.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Eleonora Sarantelli, Apostolis Mourkakis, Lefteris C. Zacharia, Andreas Stylianou, Vasiliki Gkretsi
Summary: As metastasis is a major cause of cancer-related deaths, understanding the cellular and molecular events involved in cancer cell migration and invasion is crucial for developing novel anti-metastatic therapies. Fascin-1, an actin-bundling protein, plays a fundamental role in cell migration processes. It is significantly elevated in most cancers and its high expression is associated with aggressive disease and poor prognosis. Recent studies show that Fascin-1 is critically involved in metastasis and suggest it as a promising target for anti-metastatic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mohamed Chamlali, Sana Kouba, Lise Rodat-Despoix, Luca Matteo Todesca, Zoltan Pethoe, Albrecht Schwab, Halima Ouadid-Ahidouch
Summary: Orai3 regulates breast cancer cell migration through a Ca2+-dependent modulation of cell adhesive capacities and a Ca2+-independent regulation of the actin cytoskeleton via focal adhesion kinase (FAK).
Article
Engineering, Biomedical
Patricia Costa, Liisa M. Blowes, Ana C. Laly, John T. Connelly
Summary: The study demonstrates that adhesive cues from the extracellular matrix guide collective epithelial polarity and migration, depending on adherens junctions and microtubules. Disruption of the microtubule network blocks collective polarization and inhibits directed migration of epithelial cells according to pre-established polarity.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Laura Bozal-Basterra, Maria Gonzalez-Santamarta, Veronica Muratore, Natalia Martin-Martin, Amaia Ercilla, Jose A. Rodriguez, Arkaitz Carracedo, James D. Sutherland, Rosa Barrio
Summary: LUZP1 exhibits frequent genomic aberrations in cancer, particularly gene deletions, and its loss promotes cell migration, invasion, apoptosis, and alterations in cell morphology. Additionally, LUZP1 plays regulatory roles beyond actin filament bundling, affecting actin polymerization through changes in ACTR3 and phospho-cofilin ratios. These findings suggest a novel role for LUZP1 in cancer regulation through actin cytoskeleton control.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Jan Schick, Erez Raz
Summary: In the context of cell development, migration, and pathological conditions, cells commonly form protrusions called blebs. The inflation of blebs depends on actomyosin contraction, and they play an important role in cell locomotion. Different cells produce different forms of protrusions, with blebs being one of them.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Frederic Larbret, Pierric Biber, Nicholas Dubois, Stoyan Ivanov, Laurence Lafanechere, Sophie Tartare-Deckert, Marcel Deckert
Summary: In this study, the Actin CytoFRET method was used to screen PTM-interfering compounds, and small molecule inhibitors of DUBs were found to induce actin polymerization and block cell migration. Further mechanistic investigation revealed that ROS-dependent cofilin modulation plays a key role in the regulation of actin dynamics.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Mabruka Alfaidi, Matthew L. Scott, Anthony Wayne Orr
Summary: The Nck family of adaptor proteins, Nck1 and Nck2, play crucial roles in linking phosphotyrosine signaling to changes in cytoskeletal dynamics and gene expression, affecting cellular phenotype. While they have redundant roles in vascular development and angiogenesis, recent studies suggest that Nck1 and Nck2 may differentially regulate cell phenotype in the adult vasculature. Domain-specific interactions likely mediate these isoform-selective effects, and could potentially be targeted for therapeutic interventions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Robert J. Huebner, Abdul Naseer Malmi-Kakkada, Shinuo Weng, D. Thirumalai, John B. Wallingford
Summary: Morphogenesis is regulated by the interaction of molecular signals and mechanical forces, with key factors being the local mechanical heterogeneity of cell-cell junctions. The patterning of cadherins and local mechanics along cell-cell junctions is controlled by Planar Cell Polarity signaling, a crucial genetic module for convergent extension.
Article
Multidisciplinary Sciences
Ryota Sakamoto, Ziane Izri, Yuta Shimamoto, Makito Miyazaki, Yusuke T. Maeda
Summary: Cell migration in confined environments is crucial for various biological processes. This study investigates the physical determinants of actomyosin-based migration in confined environments using an in vitro migratory cell model. The results reveal the importance of the physical interaction between actomyosin networks and the cell membrane in generating a propulsive force for cell movement.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Matthew E. Berginski, Nienke Moret, Changchang Liu, Dennis Goldfarb, Peter K. Sorger, Shawn M. Gomez
Summary: Kinases play crucial roles in cell signaling pathways, with known dysfunctions being linked to various pathologies. However, the functions of nearly one-third of kinases remain largely unknown. The Dark Kinase Knowledgebase (DKK) aims to provide data and reagents for these understudied kinases, supporting the broader research community in exploring potentially high-impact druggable targets.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Benjamin W. Neuman, Wesley A. Brashear, Marcel Brun, Sankar P. Chaki, Rebecca S. B. Fischer, Sierra J. Guidry, Joshua E. Hill, Andrew E. Hillhouse, Charles D. Johnson, Melissa M. Kahl-McDonagh, Richard P. Metz, Allison C. Rice-Ficht, Jennifer A. Shuford, Tiffany A. Skaggs, Matthew A. Stull, David W. Threadgill, Yao Akpalu, Kurt Zuelke
Summary: The study identified three novel viral variants in a college campus population, indicating the potential of undervaccinated younger individuals as a source of spread and generation of new variants. Whole genome sequencing proved to be a valuable routine disease surveillance tool.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Engineering, Biomedical
Sebastian Mestril, Raehyun Kim, Samuel S. Hinman, Shawn M. Gomez, Nancy L. Allbritton
Summary: The study demonstrated that colonic epithelial cultures enriched for stem/proliferative cells exhibit higher frequency and shorter pulse width of Ca2+ transients, and differentiation leads to a decrease in transient frequency by 50% and an increase in pulse width by 200%. Various metabolites and growth factors can modulate Ca2+ oscillation patterns consistently, while increasing the stiffness of the supportive matrix shifts Ca2+ transient patterns toward those resembling differentiated cells.
ADVANCED HEALTHCARE MATERIALS
(2021)
Correction
Immunology
Jamie S. Benn, Sankar P. Chaki, Yi Xu, Thomas A. Ficht, Allison C. Rice-Ficht, Walter E. Cook
Article
Biochemical Research Methods
Kathryn E. Kirchoff, Shawn M. Gomez
Summary: Motivation: Phosphorylation is crucial for cellular signaling, but the specific kinases involved in phosphorylation events are largely unknown. This study presents a deep learning method, EMBER, that integrates kinase phylogenetic information and motif-dissimilarity information to predict kinase-motif phosphorylation events, improving the known map of phosphorylation relationships.
Article
Biochemistry & Molecular Biology
Rex E. Jeffries, Shawn M. Gomez, Jeffrey M. Macdonald, Michael P. Gamcsik
Summary: Nuclear magnetic resonance (NMR) spectroscopy was used to monitor the metabolism of glutathione in alginate-encapsulated JM-1 hepatoma cells. The results showed that the C-13-labeled glutathione signal provided a high-intensity spectrum, allowing for the monitoring of the detailed kinetics of glutathione metabolism. Additionally, the effects of the thiol alkylating agent on glutathione metabolism were observed, and the regeneration of intracellular glutathione was studied through the restoration of the C-13-glutathione signal and the wash-out of the conjugate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Gastroenterology & Hepatology
Kevin A. Chen, Matthew E. Berginski, Chirag S. Desai, Jose G. Guillem, Jonathan Stem, Shawn M. Gomez, Muneera R. Kapadia
Summary: This study developed and compared deep neural network (NN) models and logistic regression (LR) models for predicting complications after colectomy, hepatectomy, and pancreaticoduodenectomy (PD). The results showed that machine learning models had a slight advantage over traditional statistical techniques in predicting procedure-specific outcomes, and models that included intra-operative information performed better.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Microbiology
Sankar Prasad Chaki, Melissa M. Kahl-McDonagh, Benjamin W. Neuman, Kurt A. Zuelke
Summary: This study developed a rapid and cost-effective strategy for monitoring SARS-CoV-2 variants using spike protein receptor-binding-motif (RBM)-targeted Sanger sequencing in saliva and nasal swab samples.
MICROBIOLOGY SPECTRUM
(2022)
Article
Gastroenterology & Hepatology
Kevin A. Chen, Chinmaya U. Joisa, Karyn B. Stitzenberg, Jonathan Stem, Jose G. Guillem, Shawn M. Gomez, Muneera R. Kapadia
Summary: Machine learning approaches have shown superiority in predicting readmission after colorectal surgery compared to traditional statistical methods.
JOURNAL OF GASTROINTESTINAL SURGERY
(2022)
Article
Gastroenterology & Hepatology
Kevin A. Chen, Chinmaya U. Joisa, Jonathan M. Stem, Jose G. Guillem, Shawn M. Gomez, Muneera R. Kapadia
Summary: This study used machine learning techniques to develop a more accurate predictive model for colorectal surgical-site infections. Compared to logistic regression, machine learning showed higher accuracy. These techniques can be used to identify high-risk patients and target preventive interventions for surgical-site infection.
DISEASES OF THE COLON & RECTUM
(2023)
Article
Surgery
Kevin A. Chen, Chinmaya U. Joisa, Jonathan M. Stem, Jose G. Guillem, Shawn M. Gomez, Muneera R. Kapadia
Summary: Machine learning models showed higher accuracy in predicting ureteral injury during colorectal surgery compared to logistic regression and previous models, which can support decision making regarding preoperative ureteral stent placement.
Article
Surgery
Logan R. Butler, Kevin A. Chen, Justin Hsu, Muneera R. Kapadia, Shawn M. Gomez, Timothy M. Farrell
Summary: Machine learning algorithms outperform logistic regression in predicting readmissions following bariatric surgery, offering higher accuracy and discrimination. The application of these models can help identify candidates for early discharge or targeted postdischarge resources.
SURGERY FOR OBESITY AND RELATED DISEASES
(2023)
Article
Biochemical Research Methods
Matthew Berginski, Chinmaya Joisa, Brian Golitz, Shawn Gomez
Summary: Protein kinases are important in cellular processes, and compounds inhibiting their activity are a focus in targeted therapy development, particularly in cancer. This study combines kinase inhibitor profiles and gene expression data to predict cell viability screening results, achieving high prediction accuracy. The results demonstrate that knowledge of the kinome can predict specific cell phenotypes, and can be integrated into targeted therapy development.
PLOS COMPUTATIONAL BIOLOGY
(2023)
Article
Oncology
Kevin A. Chen, Kathryn E. Kirchoff, Logan R. Butler, Alexa D. Holloway, Muneera R. Kapadia, Cherie M. Kuzmiak, Stephanie M. Downs-Canner, Phillip M. Spanheimer, Kristalyn K. Gallagher, Shawn M. Gomez
Summary: An artificial intelligence model was developed to predict the pathologic margin status of resected breast tumors using specimen mammography. The model showed high accuracy and potential to guide the extent of resection, improving cosmesis and reducing reoperations.
ANNALS OF SURGICAL ONCOLOGY
(2023)
Article
Gastroenterology & Hepatology
Kevin A. Chen, Paolo Goffredo, David Hu, Chinmaya U. Joisa, Jose G. Guillem, Shawn M. Gomez, Muneera R. Kapadia
Summary: Machine learning-based models were developed to predict outcomes in anal squamous cell carcinoma (ASCC), showing higher accuracy compared to logistic regression for locoregional failure. These models may assist clinicians in identifying high-risk ASCC patients after external validation.
JOURNAL OF GASTROINTESTINAL SURGERY
(2023)
