期刊
JOURNAL OF CELL SCIENCE
卷 126, 期 2, 页码 593-604出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.115725
关键词
Atg; Atg18; Atg2; autophagy; phagophore assembly site; phosphoinositides
类别
资金
- Netherlands Organization for Health Research and Development (ZonMW) VIDI [917.76.329]
- Chemical Sciences (CW) ECHO [700.59.003]
- Earth and Life Sciences (ALW) Open Program [821.02.017]
- Deutsche Forschungsgemeinschaft (DFG)-Netherlands Organisation for Scientific Research (NWO) cooperation [DN82-303/UN111/7-1]
- DFG [SFB 773 (A3)]
Autophagy is a conserved degradative transport pathway. It is characterized by the formation of double-membrane autophagosomes at the phagophore assembly site (PAS). Atg18 is essential for autophagy but also for vacuole homeostasis and probably endosomal functions. This protein is basically a beta-propeller, formed by seven WD40 repeats, that contains a conserved FRRG motif that binds to phosphoinositides and promotes Atg18 recruitment to the PAS, endosomes and vacuoles. However, it is unknown how Atg18 association with these organelles is regulated, as the phosphoinositides bound by this protein are present on the surface of all of them. We have investigated Atg18 recruitment to the PAS and found that Atg18 binds to Atg2 through a specific stretch of amino acids in the beta-propeller on the opposite surface to the FRRG motif. As in the absence of the FRRG sequence, the inability of Atg18 to interact with Atg2 impairs its association with the PAS, causing an autophagy block. Our data provide a model whereby the Atg18 beta-propeller provides organelle specificity by binding to two determinants on the target membrane.
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