Review
Cell Biology
Jaroslav Kalous, Daria Aleshkina, Martin Anger
Summary: Akt, also known as protein kinase B (PKB), is a crucial factor in the PI3K/Akt signaling pathway that regulates cell survival, metabolism, and proliferation. Akt phosphorylates downstream substrates to control various cellular processes, including nuclear envelope breakdown, centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. Akt also plays important roles in oogenesis, preimplantation development, and the regulation of mRNA translation through the mTORC1 pathway.
Article
Cell Biology
Juan Ge, Na Zhang, Shoubin Tang, Feifei Hu, Xiaojing Hou, Hongzheng Sun, Longsen Han, Qiang Wang
Summary: Maternal diabetes impairs oocyte quality, but controlling the PDK1-mediated Ser232-PDHE1α phosphorylation pathway can partly protect oocytes from the effects of diabetes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Lin-Lin Hu, Meng-Hao Pan, Feng-Lian Yang, Zi-Ao Zong, Feng Tang, Zhen-Nan Pan, Xiang Lu, Yan-Ping Ren, Jun-Li Wang, Shao-Chen Sun
Summary: Research demonstrates that FASCIN is crucial for mouse oocyte maturation, affecting spindle migration and polar body extrusion. It may be regulated by PKC and plays a significant role in meiosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Genetics & Heredity
Jessica E. Fellmeth, Kim S. McKim
Summary: Drosophila has been a valuable model organism for studying meiosis, particularly in females, due to its genetic and cytological tools. It is also a good model for mammalian meiosis due to biological similarities.
Article
Reproductive Biology
Ying Xu, Chang -Long Xu, Zhong-Feng Xu, Xin-Jie Wang, Hui-Sheng Liang, Zhao-Cheng Zeng, Li-Xin Zeng, Kang-Na Wei, Shu-Zi Deng, Shu-Juan Xie, Jiang Jiang, Yu-Xin Liu, Yun-Kao Cao, Hai-Long Wang
Summary: Fbf1 plays a crucial role in spindle assembly and chromosome arrangement during mouse oocyte meiosis, affecting the progression of cell cycle.
Article
Cell Biology
Jian Li, Hao-Ya Chang, Zi-Yun Yi, Chun-Hui Zhang, Qing-Yuan Sun, Wei-Ping Qian
Summary: Studies on mammalian oocytes have shown that CDK2 activity plays a crucial role in the proper dynamics of spindle and chromosomes, with CDK2 inhibition leading to continuous SAC activation and subsequent inactivation of APC/C activity.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Shuo Chao, Li -Jun Li, Jun Lu, Shu-Xian Zhao, Ming -Hui Zhao, Gui-An Huang, Shen Yin, Wei Shen, Qing-Yuan Sun, Yong Zhao, Zhao-Jia Ge, Lei Zhao
Summary: This study evaluated the effect of EGCG on the maturation of diabetic oocytes. The results showed that EGCG could reduce the frequency of abnormal spindle assembly and chromosome mismatches in diabetic oocytes. Additionally, EGCG alleviated abnormal mitochondrial membrane potential, regulated the expression of genes involved in mitochondrial fusion and fission, reduced reactive oxygen species levels, and decreased DNA damage in diabetic oocytes.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Cell Biology
Zhen-Nan Pan, Jing-Cai Liu, Jia-Qian Ju, Yue Wang, Shao-Chen Sun
Summary: This study demonstrates the critical roles of LRRK2 in mammalian oocyte meiosis, primarily through regulating actin assembly for spindle migration and maintaining mitochondrial function.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Cell Biology
Yuan-Jing Zou, Meng-Meng Shan, Hong-Hui Wang, Zhen-Nan Pan, Meng-Hao Pan, Yi Xu, Jia-Qian Ju, Shao-Chen Sun
Summary: Our study investigated the roles of RAB14 during oocyte meiotic maturation, showing that depletion of RAB14 caused large polar bodies and defects in spindle migration. The regulation on actin-based spindle migration and Golgi apparatus distribution by RAB14 during mouse oocyte meiotic maturation was suggested by our results.
CELL PROLIFERATION
(2021)
Article
Biochemistry & Molecular Biology
Guangyi Cao
Summary: The correct assembly and regulation of the spindle apparatus are crucial for the precise separation of chromosomes in mouse oocytes. NUSAP1 is a spindle-associated protein that plays an important role in oocyte meiosis maturation. Its depletion leads to chromosome misalignment, aneuploidy, and abnormal spindle assembly.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Reproductive Biology
Qingqing Cai, Keying Wen, Miao Ma, Wei Chen, Delin Mo, Zuyong He, Yaosheng Chen, Peiqing Cong
Summary: EZH2 plays a crucial role in porcine oocyte meiotic maturation by regulating spindle organization, maintaining chromosomal integrity, and mitochondrial function.
BIOLOGY OF REPRODUCTION
(2021)
Article
Biochemistry & Molecular Biology
Jia-Qian Ju, Zhen-Nan Pan, Kun-Huan Zhang, Yi-Ming Ji, Jing-Cai Liu, Shao-Chen Sun
Summary: This study demonstrates that Mcrs1 plays important roles in regulating the meiotic G2/M transition and spindle assembly in mouse oocytes. Mcrs1 is present in the nucleus and associates with spindle poles and chromosomes during meiosis I. Depletion of Mcrs1 affects histone modifications, CDK1 activity, and cyclin B1 accumulation, leading to delayed G2/M transition and abnormal spindle assembly.
Article
Cell Biology
Hong Yin, Teng Zhang, Hao Wang, Xin Hu, Xuan Hou, Xianbao Fang, Yaoxue Yin, Hui Li, Lanying Shi, You-Qiang Su
Summary: The research reveals that EML1 plays a crucial role in controlling oocyte meiotic progression in mice, and its loss leads to abnormal oocytes unable to progress to the second meiotic division.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Jaroslav Kalous, Daria Aleshkina
Summary: Cells contain a diverse network of signaling and regulatory proteins to ensure the proper progression of cell division. A key regulator in this process is the enzyme polo-like kinase 1 (PLK1), which plays important roles in mitosis, meiosis, and cytokinesis. PLK1 controls various aspects of cell division, including nuclear envelope breakdown, centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. In mammalian oogenesis, PLK1 is essential for the resumption of meiosis and establishment of the meiotic spindle. Additionally, PLK1 regulates localized mRNA translation by phosphorylating and inhibiting the translational repressor 4E-BP1, a downstream target of the mTOR pathway.
Article
Cell Biology
Pedro Barbosa, Liudmila Zhaunova, Simona Debilio, Verdiana Steccanella, Van Kelly, Tony Ly, Hiroyuki Ohkura
Summary: Posttranslational modifications phosphorylation and ubiquitination cooperate to promote synaptonemal complex assembly, with the ubiquitin ligase complex SCF playing a key role by down-regulating the phosphatase subunit PP2A-B56. This regulation is mediated by F-box proteins Slmb/beta Trcp and Fbxo42, highlighting the importance of these proteins in maintaining genetic diversity during meiosis.
JOURNAL OF CELL BIOLOGY
(2021)
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: Behavioral analysis has evolved from manual annotation to automation, and further advancements are expected in the future.
Editorial Material
Biochemical Research Methods
Lei Tang
Summary: The assembloids, which are generated by spatially organizing multiple cell types, will provide deeper insights into tissue function.
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: iGECI, a near-infrared calcium sensor, shows promise for imaging neuronal activity in vivo.
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: DeepLabCut-Live! performs real-time pose estimation and enables closed-loop feedback on behavior.
Editorial Material
Biochemical Research Methods
Nina Vogt
Editorial Material
Biochemical Research Methods
Nina Vogt
Editorial Material
Biochemical Research Methods
Nina Vogt
Editorial Material
Biochemical Research Methods
Nina Vogt
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: The approach of barcoded viral tracing allows insights into cell-cell interactions at single-cell resolution.
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: The resolution of atomic force microscopy can be increased by applying principles from super-resolution fluorescence microscopy.
Editorial Material
Biochemical Research Methods
Nina Vogt
Summary: The DELIVER approach allows for the directed evolution of adeno-associated virus capsids to generate tissue-specific viruses.
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Biochemical Research Methods
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News Item
Biochemical Research Methods
Arunima Singh
Editorial Material
Biochemical Research Methods
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