Article
Biochemistry & Molecular Biology
Pengbo Liang, Clara Schmitz, Beatrice Lace, Franck Anicet Ditengou, Chao Su, Eija Schulze, Julian Knerr, Robert Grosse, Jean Keller, Cyril Libourel, Pierre-Marc Delaux, Thomas Ott
Summary: Legumes have the ability to associate with rhizobia for nitrogen-fixing root nodule symbiosis. In Medicago truncatula, root hair colonization by Sinorhizobium meliloti begins from young root hairs, which physically entwine around the symbiont to form rhizobial traps. This unique process involves alterations in membrane organization, cytosolic calcium gradient, actin rearrangements, and symbiotic responses in legumes.
Article
Cell Biology
Istvan Foldi, Krisztina Toth, Rita Gombos, Peter Gaszler, Peter Gorog, Ioannis Zygouras, Beata Bugyi, Jozsef Mihaly
Summary: Research has shown that DAAM is the predominant formin involved in axonal growth in Drosophila, and its actin-processing activity is essential for axonal growth. Additionally, a novel MT-binding motif within the FH2 domain of DAAM has been identified, which is required for proper growth and guidance of mushroom body axons.
Review
Biochemistry & Molecular Biology
Wa Du, Lu Ren, Milton H. Hamblin, Yanbo Fan
Summary: The process of angiogenesis, involving the formation of new blood vessels from existing ones, is critical in various physiological and pathological conditions. Endothelial cell metabolism, particularly glycolysis, has been shown to play a significant role in angiogenesis, with various regulators identified to modulate this process. Understanding the metabolic regulation in endothelial cells could lead to potential therapeutic strategies for angiogenesis-related diseases.
Review
Cell Biology
Li-Kun Phng, Heinz-Georg Belting
Summary: The vertebrate cardiovascular system consists of a highly specialized blood vessel network that emerges during early embryonic development. This network is influenced by the behaviors of endothelial cells driven by actin and hemodynamic forces from pressurized blood flow. While primarily focusing on evidence from zebrafish embryos, complementary findings from other experimental systems are also mentioned.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ana Martins Figueiredo, Pedro Barbacena, Ana Russo, Silvia Vaccaro, Daniela Ramalho, Andreia Pena, Aida Pires Lima, Rita Rua Ferreira, Marta Alves Fidalgo, Fatima El-Marjou, Yulia Carvalho, Francisca Ferreira Vasconcelos, Ana-Maria Lennon-Dumenil, Danijela Matic Vignjevic, Claudio Areias Franco
Summary: The study shows that endothelial tip cells use dactylopodia as the main cellular protrusion for invasion, and this process is regulated by the balance of myosin IIA and actin-related protein activity. Autonomous ablation of these proteins in endothelial cells may lead to abnormal protrusion formation, affecting angiogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
David Rabbolini, Hai Po Helena Liang, Marie-Christine Morel-Kopp, David Connor, Shane Whittaker, Scott Dunkley, Dea Donikian, Mayuko Kondo, Walter Chen, William S. Stevenson, Heather Campbell, Joanne Joseph, Christopher Ward, Timothy Brighton, Vivien M. Chen
Summary: Variants of the DIAPH1 gene have been reported to cause inherited macrothrombocytopenia, early-onset progressive sensorineural hearing loss, and mild asymptomatic neutropenia, along with alterations in procoagulant platelet formation and possible dental anomalies.
Review
Immunology
Jiajie Kuai, Chenchen Han, Wei Wei
Summary: GRK2 is a key player in various signaling networks, interacting with both GPCRs and non-GPCR substrates to regulate angiogenesis. Changes in GRK2 expression and localization have been linked to the angiogenesis process in different diseases, suggesting its potential as a target for anti-angiogenesis therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Malou Zuidscherwoude, Elizabeth J. Haining, Victoria A. Simms, Stephanie Watson, Beata Grygielska, Alex T. Hardy, Andrea Bacon, Stephen P. Watson, Steven G. Thomas
Summary: The formin family plays a critical role in cytoskeletal dynamics and platelet formation, with a specific importance in megakaryocyte function. Knockout mice lacking two formin proteins exhibit macrothrombocytopenia due to aberrant megakaryocyte function and a slight decrease in platelet lifespan, while platelet function remains unaffected.
Article
Multidisciplinary Sciences
Heidi Ulrichs, Ignas Gaska, Shashank Shekhar
Summary: Cells regulate actin assembly by controlling reactions at actin filament barbed ends. Three proteins, formin, CP, and twinfilin, have distinct roles at these barbed ends. Using microfluidics-assisted TIRF microscopy, it was found that all three proteins can bind filament barbed ends simultaneously. Single-molecule experiments showed that twinfilin cannot bind barbed ends occupied by formin in the presence of CP. This study establishes a paradigm where polymerases, depolymerases, and cappers together tune actin assembly.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Shuang Zhang, Bastiaan Tuk, Jeroen Van de Peppel, Gert-Jan Kremers, Marijke Koedam, Georg R. Pesch, Zaid Rahman, Remco M. Hoogenboezem, Eric M. J. Bindels, Johan W. Van Neck, Pouyan E. Boukany, Johannes P. T. M. Van Leeuwen, Bram C. J. Van der Eerden
Summary: A functional vascular system is crucial for bone repair, and disrupted angiogenesis can lead to non-union. Paracrine factors released by human bone marrow derived mesenchymal stromal cells (BM-SCs) have angiogenic effects on endothelial cells. This study used microfluidic designs to investigate angiogenesis and found that BMSC conditioned medium (CM) significantly promoted endothelial cell proliferation, chemotactic and mechanotactic migration. Transcriptional analysis of endothelial cells revealed unique gene expression related to tricarboxylic acid cycle and energy metabolism when exposed to both BMSC-CM and shear stress. This study highlights the importance of considering in vivo-like microenvironments when studying vessel repair during fracture healing.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Diana Kim, Alexander Grath, Yao Wei Lu, Karl Chung, Max Winkelman, John J. Schwarz, Guohao Dai
Summary: Sox17 plays a critical role in regulating arterial identity during embryonic vascular development. Its impact on adult endothelial cells (ECs) is examined in this study. It is found that Sox17-induced ECs exhibit gene profile favoring arterial and tip cell identity, and they demonstrate greater adaptability in response to fluid shear stress. Sox17-mediated ECs also display distinct morphogenic behaviors during vessel formation.
Review
Biochemistry & Molecular Biology
Lucio Diaz-Flores, Ricardo Gutierrez, Maria Pino Garcia, Miriam Gonzalez-Gomez, Lucio Diaz-Flores, Jose Luis Carrasco, Juan Francisco Madrid, Aixa Rodriguez Bello
Summary: Perivascular cells, including pericytes and CD34+ stromal cells/telocytes, play an important role in angiogenesis. The behavior and changes of these cells differ depending on the type of angiogenesis, involving various cell-to-cell interactions and signaling pathways. Perivascular cells and CD34+ stromal cells/telocytes have important functions in vessel development and tissue repair, while their involvement in intussusceptive angiogenesis is limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Nathalie Tisch, Carmen Ruiz de Almodovar
Summary: The formation of new blood vessels involves proliferation of endothelial cells and vessel sprout elongation, along with vessel remodeling and regression to adapt vessel density. Recent studies focus on how vessel regression and cell death contribute to this process, challenging the simplistic view of cell death machinery as only promoting cellular demise. This review highlights the role of cell death signaling pathways, particularly apoptosis and necroptosis, in blood vessel formation during development and pathology.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Yi-Nan Lee, Hsueh-Hsiao Wang, Cheng-Huang Su, Hsin- Lee, Yen-Hung Chou, Chin-Ling Hsieh, Wen-Ting Liu, Kuo-Tung Shu, Kai-Ting Chang, Hung- Yeh, Yih-Jer Wu
Summary: The study established an accelerated senescence model by treating endothelial progenitor cells (EPCs) with the iron chelator deferoxamine (DFO). Results showed that DFO treatment induced senescent phenotypes in EPCs and could validate its effects in vivo.
Article
Biochemistry & Molecular Biology
Anh-Nhung Le, Seong-Soon Park, Minh-Xuan Le, Unn Hwa Lee, Byung Kyun Ko, Hye Ryeong Lim, Ri Yu, Seong Hee Choi, Byung Ju Lee, Soo-Youn Ham, Chang Man Ha, Jeong Woo Park
Summary: Deficiency of DRG2 induces senescence and dysfunction of endothelial cells, leading to impaired angiogenesis and reduced blood flow. DRG2 deficiency upregulates arginase 2 and reactive oxygen species generation in endothelial cells. These findings highlight the significant role of DRG2 in endothelial cell senescence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Tak Shun Fung, Wei-Ke Ji, Henry N. Higgs, Rajarshi Chakrabarti
JOURNAL OF CELL SCIENCE
(2019)
Review
Oncology
Supriya Borah, Dileep Vasudevan, Rajeeb K. Swain
Article
Biochemistry & Molecular Biology
Daniel R. Sandoval, Alejandro Gomez Toledo, Chelsea D. Painter, Ember M. Tota, M. Osman Sheikh, Alan M. West, Martin M. Frank, Lance Wells, Ding Xu, Roy Bicknell, Kevin D. Corbett, Jeffrey D. Esko
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
A. Mu, Tak Shun Fung, Lisa M. Francomacaro, Thao Huynh, Tommi Kotila, Zdenek Svindrych, Henry N. Higgs
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Peripheral Vascular Disease
Asha L. Bayliss, Ananthalakshmy Sundararaman, Camille Granet, Harry Mellor
Article
Biochemistry & Molecular Biology
Ananthalakshmy Sundararaman, Yoko Fukushima, Jim C. Norman, Akiyoshi Uemura, Harry Mellor
Article
Endocrinology & Metabolism
Ashton Faulkner, Anita Tamiato, William Cathery, Andrea Rampin, Carlo Maria Caravaggi, Eva Jover, Steve Allen, Harry Mellor, David Hauton, Lisa C. Heather, Gaia Spinetti, Paolo Madeddu
Article
Pathology
Joseph Robinson, Katharine Whitworth, Elizabeth Jinks, Zsuzsanna Nagy, Roy Bicknell, Steven P. Lee
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2020)
Article
Medicine, Research & Experimental
Xiaodong Zhuang, Federica Maione, Joseph Robinson, Michael Bentley, Baksho Kaul, Katharine Whitworth, Neeraj Jumbu, Elizabeth Jinks, Jonas Bystrom, Pietro Gabriele, Elisabetta Garibaldi, Elena Delmastro, Zsuzsanna Nagy, David Gilham, Enrico Giraudo, Roy Bicknell, Steven P. Lee
Article
Biochemistry & Molecular Biology
Tak Shun Fung, Rajarshi Chakrabarti, Jana Kollasser, Klemens Rottner, Theresia E. B. Stradal, Frieda Kage, Henry N. Higgs
Summary: Mitochondrial damage triggers metabolic changes and mitophagy in cells. This study reveals the mechanism of a rapid peri-mitochondrial actin polymerization called acute damage-induced actin (ADA). The activation of ADA involves multiple signaling pathways and proteins, and it inhibits the rapid mitochondrial shape changes that occur upon mitochondrial depolarization.
Article
Cell Biology
Joseph W. Hawkins, Madeleine C. McNeill, Reza Ebrahimighaei, Harry H. Mellor, Andrew C. Newby, Mark Bond
Summary: cAMP inhibits NF-kB activity by increasing nuclear actin monomer levels, leading to immunosuppressive and anti-inflammatory effects.
Article
Cell Biology
Rajarshi Chakrabarti, Tak Shun Fung, Taewook Kang, Pieti W. Elonkirjo, Anu Suomalainen, Edward J. Usherwood, Henry N. Higgs
Summary: Chakrabarti, Fung et al. demonstrate that actin polymerization dependent on the Arp2/3 complex is necessary for the rapid increase of glycolysis under conditions that compromise mitochondrial function. They also suggest that acute damage-induced actin (ADA) is linked to the glycolytic activation upon mitochondrial damage.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Barbara Calabrese, Steven Jones, Yoko Yamaguchi-Shiraishi, Michael Lingelbach, Uri Manor, Tatyana M. Svitkina, Henry N. Higgs, Andy Y. Shih, Shelley Halpain
Summary: Ischemia and excess NMDA receptor activation cause actin to reorganize, protecting neurons from death induced by cell edema.
NATURE COMMUNICATIONS
(2022)
Review
Cell Biology
Tak Shun Fung, Rajarshi Chakrabarti, Henry N. N. Higgs
Summary: Actin plays multiple roles in mitochondrial biology, including mitochondrial fission, regulating organelle function and dynamics, and affecting mitochondrial motility. Actin can promote mitochondrial fission through polymerization from the endoplasmic reticulum or the Arp2/3 complex. It also plays a role in mitophagy and can both stimulate and inhibit mitochondrial motility.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Correction
Cell Biology
Tak Shun Fung, Rajarshi Chakrabarti, Henry N. Higgs
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)