Article
Mathematics, Applied
Congping Lin, Peter Ashwin
Summary: The Endoplasmic Reticulum (ER) is a complex dynamic network of filaments in the viscous cytoplasm of plant cells, connected to the cell membrane. A dynamical model of the ER network as constant tension filaments subject to viscous forces is proposed. The network exhibits foam-like characteristics and can retain memory of previous flow direction and intensity.
COMMUNICATIONS IN NONLINEAR SCIENCE AND NUMERICAL SIMULATION
(2023)
Article
Multidisciplinary Sciences
Ying Zhang, Evelina De Laurentiis, Katherine E. Bohnsack, Mascha Wahlig, Namit Ranjan, Simon Gruseck, Philipp Hackert, Tina Wolfle, Marina Rodnina, Blanche Schwappach, Sabine Rospert
Summary: The GET pathway aids in delivering tail-anchored proteins to the ER, with Get4/5 components probing near the ribosomal exit tunnel and recruiting Sgt2 upon emergence of client proteins to initiate the targeting phase of the pathway.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sana A. Bentebbal, Bakhita R. Meqbel, Anna Salter, Victoria Allan, Brian Burke, Henning F. Horn
Summary: KASH5 is a recently discovered protein involved in meiosis. A variant in human KASH5 (L535Q) has been linked to male infertility and azoospermia, possibly due to its effect on protein subcellular localization.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yun Xiang, Rui Lyu, Junjie Hu
Summary: Membrane curvature is generated by constructing a curved oligomeric scaffold formed by endoplasmic reticulum tubule-forming proteins. The mechanism of curvature generation remains elusive.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Cesar Bernat-Silvestre, Judit Sanchez-Simarro, Yingxuan Ma, Javier Montero-Pau, Kim Johnson, Fernando Aniento, Maria Jesus Marcote
Summary: Glycosylphosphatidylinositol (GPI)-anchored proteins are crucial for various plant processes, and lipid remodeling is essential for their export to the cell surface. Arabidopsis PGAP1 acts as a GPI inositol-deacylase, facilitating the efficient ER export and transport of GPI-APs.
Article
Chemistry, Multidisciplinary
Huiying Mu, Koji Miki, Takuya Kubo, Koji Otsuka, Kouichi Ohe
Summary: The new thiol probe VB1Cl, based on the meso-vinyl-BODIPY scaffold, showed significant fluorescence enhancement and high selectivity for biological thiol sensing. It was successfully utilized to monitor the protein unfolding process in living cells under ER stress.
CHEMICAL COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Melanie A. Mcdowell, Michael Heimes, Giray Enkavi, Akos Farkas, Daniel Saar, Klemens Wild, Blanche Schwappach, Ilpo Vattulainen, Irmgard Sinning
Summary: This study reveals the structure and functional characteristics of the guided entry of tail-anchored proteins (GET) insertase, including its remodeling of the membrane environment to promote substrate insertion.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Jonas C. Alvim, Robert M. Bolt, Jing An, Yasuko Kamisugi, Andrew Cuming, Fernanda A. L. Silva-Alvim, Juan O. Concha, Luis L. P. da Silva, Meiyi Hu, Dominique Hirsz, Jurgen Denecke
Summary: In contrast to the current model based on fast recycling of the K/HDEL receptor (ERD2) between the ER and the Golgi apparatus, the authors show evidence that ERD2 is specifically retained in the Golgi apparatus and evades joining its ligands en route to the ER.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Jacob A. Culver, Malaiyalam Mariappan
Summary: The study found that tail-anchored proteins are recognized and ubiquitinated by cytosolic protein quality control system upon synthesis, but are not degraded by proteasomes. Instead, they are delivered to the ER for insertion, where deubiquitinases USP20 and USP33 remove ubiquitin chains.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Keisuke Mochida, Toshifumi Otani, Yuto Katsumata, Hiromi Kirisako, Chika Kakuta, Tetsuya Kotani, Hitoshi Nakatogawa
Summary: In this study, the researchers uncovered the mechanism of action of the nucleophagy receptor Atg39 in the formation of NDVs. Atg39 is anchored to the outer nuclear membrane via its transmembrane domain and is also associated with the inner nuclear membrane through membrane-binding amphipathic helices, linking these membranes together. Additionally, the aggregation of Atg39 causes the nuclear envelope to protrude and pinch off to generate NDVs.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael J. McKenna, Benjamin M. Adams, Vincent Chu, Joao A. Paulo, Sichen Shao
Summary: The study reveals that ATP13A1 plays a crucial role in preventing ER-associated degradation of mislocalized and misoriented proteins. Without ATP13A1, mitochondrial tail-anchored proteins mislocalize to the ER and are degraded, and a subset of proteins with specific signal sequences or transmembrane segments accumulate in the wrong orientation and are targeted for degradation.
Article
Chemistry, Applied
Shane M. Hickey, Ian R. D. Johnson, Elena Dallerba, Mark J. Hackett, Massimiliano Massi, Joanna Lazniewska, Lauren A. Thurgood, Frederick M. Pfeffer, Douglas A. Brooks, Trent D. Ashton
Summary: We introduce a highly fluorescent, solvatochromic, 1,8-naphthalimide probe that enables the simultaneous imaging of lipid droplets and endoplasmic reticulum, with distinguishable emission maxima. By delineating organelles based on their emission profiles, we are able to visualize lipid droplet biogenesis and intercellular trafficking events. Furthermore, this probe demonstrates potential histochemical applications by staining myelin lipids in ex vivo brain tissue.
Article
Cell Biology
Sofia Rodriguez-Gallardo, Susana Sabido-Bozo, Atsuko Ikeda, Misako Araki, Kouta Okazaki, Miyako Nakano, Auxiliadora Aguilera-Romero, Alejandro Cortes-Gomez, Sergio Lopez, Miho Waga, Akihiko Nakano, Kazuo Kurokawa, Manuel Muniz, Kouichi Funato
Summary: Our study reveals that the presence of C26 ceramide in the endoplasmic reticulum membrane drives clustering and sorting of GPI-APs, and the C26 ceramide is also incorporated into the lipid moiety of GPI-APs. Additionally, the GPI-glycan remodelase Ted1 monitors GPI-APs with a C26 ceramide moiety, which is necessary for receptor-mediated export of GPI-APs.
Review
Oncology
Delphine Fessart, Jacques Robert, Cecile Hartog, Eric Chevet, Frederic Delom, Guillaume Babin
Summary: Epithelial ovarian cancer (EOC) is the most common gynecologic disorder and despite recent advances made in new therapeutics, it remains the most lethal gynecologic malignancy. The discovery of anterior gradient proteins AGR2 and AGR3, members of the protein disulfide isomerase (PDI) family, has attracted attention for their potential involvement in adenocarcinoma development. Understanding the role of the AGR family may offer new avenues for handling EOC patients.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jing Yang, Tetsuya Hirata, Yi-Shi Liu, Xin-Yu Guo, Xiao-Dong Gao, Taroh Kinoshita, Morihisa Fujita
Summary: Many GPI-APs like CD59, CD55, and CD109 utilize the hSND2-dependent ER targeting machinery, with signal recognition particle receptors cooperating to facilitate this process. The hydrophobicity of the C-terminal GPI attachment signal serves as a determinant of hSND2 dependency in ER targeting of GPI-APs.