Review
Plant Sciences
Qingchen Rui, Xiaoyun Tan, Feng Liu, Yiqun Bao
Summary: In this review, we summarize the key players in Golgi maintenance demonstrated by genetic studies in plants, with an emphasis on their roles in intra-Golgi trafficking.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Cell Biology
Petia Adarska, Luis Wong-Dilworth, Francesca Bottanelli
Summary: Molecular switches of the ADP-ribosylation factor (ARF) GTPase family play crucial roles in coordinating intracellular trafficking, including membrane deformation, cargo sorting, and recruitment of downstream coat proteins and effectors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yoshinori Marunaka, Katsumi Yagi
Summary: This study investigates the intracellular protein trafficking processes using mathematical equations, exploring the implications of real and complex roots on the protein quantities in different states. Real roots correspond to monotonic changes, while complex roots lead to oscillatory changes, indicating the essential role of complex numbers in oscillatory phenomena in bodily and cellular functions.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Review
Neurosciences
Martina Polenghi, Elena Taverna
Summary: Cell polarity plays an important role in neural stem and progenitor cells, but its relationship with intracellular trafficking and glycosylation remains to be explored. Studies on neurons have shaped our understanding of intracellular trafficking and polarity. Rare diseases such as congenital disorders of glycosylation offer a unique opportunity to study the contribution of trafficking and glycosylation in neurodevelopment.
FRONTIERS IN NEUROSCIENCE
(2023)
Review
Cell Biology
Saara Laulumaa, Markku Varjosalo
Summary: The Commander complex is made up of 16 proteins and plays multiple roles in various intracellular events, cell homeostasis, cell cycle regulation, and immune response. These ubiquitously expressed proteins have been linked to diseases like Wilson's disease, atherosclerosis, and cancer. The Commander complex may play a more significant role in intracellular regulation than currently understood, requiring more systematic research.
Review
Biochemistry & Molecular Biology
Stephanie E. Crilly, Manojkumar A. Puthenveedu
Summary: GPCRs are integral membrane proteins that transduce signals from both the cell surface and intracellular membrane compartments, producing functional effects that differ from canonical plasma membrane signaling. The activity and signaling of GPCRs are influenced by intracellular membranes and their associated lipids and proteins, resulting in a spatial bias in GPCR signaling.
JOURNAL OF MEMBRANE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Riccardo Rizzo, Domenico Russo, Kazuo Kurokawa, Pranoy Sahu, Bernadette Lombardi, Domenico Supino, Mikhail A. Zhukovsky, Anthony Vocat, Prathyush Pothukuchi, Vidya Kunnathully, Laura Capolupo, Gaelle Boncompain, Carlo Vitagliano, Federica Zito Marino, Gabriella Aquino, Daniela Montariello, Petra Henklein, Luigi Mandrich, Gerardo Botti, Henrik Clausen, Ulla Mandel, Toshiyuki Yamaji, Kentaro Hanada, Alfredo Budillon, Franck Perez, Seetharaman Parashuraman, Yusuf A. Hannun, Akihiko Nakano, Daniela Corda, Giovanni D'Angelo, Alberto Luini
Summary: Research shows that a group of sequentially-acting enzymes in the Golgi bind to the oncogenic protein GOLPH3, affecting glycosphingolipid synthesis and cell proliferation by regulating enzyme localization and degradation rate. These findings reveal a novel oncogenic mechanism of action for GOLPH3 in glycosphingolipid metabolism.
Review
Biochemistry & Molecular Biology
Katarzyna M. Grochowska, Maria Andres-Alonso, Anna Karpova, Michael R. Kreutz
Summary: Synaptic function relies on the constant supply and removal of neuronal membranes. Neuronal protein transport faces challenges due to the complexity of neurons, but local micro-secretory systems have evolved to meet the requirements of neuronal membrane protein proteostasis. However, our knowledge of neuronal protein synthesis, trafficking, replacement, and degradation is limited.
Article
Cell Biology
Shaozhong Yang, Franco K. C. Au, Gefei Li, Jianwei Lin, Xiang David Li, Robert Z. Qi
Summary: Yang et al. uncover a mechanism by which the centrosomin motif 1 (CM1) regulates microtubule nucleation and cellular organization. This regulation is achieved through an autoinhibitory mechanism and can be relieved by phosphorylation. This study provides valuable insights into the control of microtubule formation in cells.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Xiuping Sun, Divyanshu Mahajan, Bing Chen, Zhiwei Song, Lei Lu
Summary: The mechanism of Golgi localization of Golgi glycosyltransferases has been found to be primarily through retention rather than retrieval. The study also revealed the contributions of different regions of ST6GAL1 to Golgi retention, showing that the N-terminal cytosolic tail and transmembrane domain can act as Golgi export signals.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Akihiro Tame, Tadashi Maruyama, Tetsuro Ikuta, Yoshihito Chikaraishi, Nanako O. Ogawa, Masashi Tsuchiya, Kiyotaka Takishita, Miwako Tsuda, Miho Hirai, Yoshihiro Takaki, Naohiko Ohkouchi, Katsunori Fujikura, Takao Yoshida
Summary: Controlling mTORC1 in mussels helps retain symbionts for nutrition, but the digestion of symbionts is triggered when the symbiotic bacteria's energy source is depleted.
Review
Biochemistry & Molecular Biology
Qingchun Lu, Po-Shun Wang, Ling Yang
Summary: This review focuses on highlighting Golgi-associated Rab GTPases as master regulators of autophagic flux, discussing the future research prospects in elucidating their role and mechanism in autophagy and autophagy-related diseases.
CELL AND BIOSCIENCE
(2021)
Article
Plant Sciences
Liang Zhang, Jingwen Ma, Huan Liu, Qian Yi, Yanan Wang, Jingjing Xing, Peipei Zhang, Shengdong Ji, Mingjun Li, Jingyuan Li, Jinbo Shen, Jinxing Lin
Summary: The R-SNAREs VAMP721 and VAMP722 in Arabidopsis are found to play crucial roles in intracellular trafficking, affecting auxin distribution and plant growth by regulating the polar localization of auxin transporters.
Article
Multidisciplinary Sciences
Carolin Haug, Florian Braig, Joachim T. Haug
Summary: The loss of biodiversity and decline of insects in modern ecosystems have a significant impact on ecological roles and economy. This study investigates the decline of lacewings since the Cretaceous, using fossil records and analysis of larvae morphology. The results indicate a severe loss of lacewings and their ecological roles over the past 100 million years.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Swapneeta S. Date, Peng Xu, Nathaniel L. Hepowit, Nicholas S. Diab, Jordan Best, Boyang Xie, Jiale Du, Eric R. Strieter, Lauren P. Jackson, Jason A. MacGurn, Todd R. Graham
Summary: This study reveals the role of COPI in SNARE trafficking and Golgi SNARE localization through ubiquitin recognition, and demonstrates how ubiquitination enhances the binding between Arf and COPI with Gos1.
Article
Cell Biology
Elisabeth Richardson, Joel B. Dacks
Summary: The ciliate phylum is a group of protists with a unique membrane trafficking system and widespread distribution. This study reveals heterogeneity in the distribution of membrane trafficking machinery in ciliates, with some protein loss indicating potential functional degeneration.
Article
Microbiology
Angana Mukherjee, Marie-Eve Crochetiere, Audrey Sergerie, Souad Amiar, L. Alexa Thompson, Zeinab Ebrahimzadeh, Dominic Gagnon, Florian Lauruol, Alexandra Bourgeois, Thomas Galaup, Stephanie Roucheray, Stephanie Hallee, Prasad K. Padmanabhan, Robert V. Stahelin, Joel B. Dacks, Dave Richard
Summary: Phosphoinositide lipids are important for various processes in eukaryotic cells, but their functions in the malaria parasite are still poorly understood. This study investigates the roles of phosphoinositides in Plasmodium falciparum and identifies a phosphoinositide-binding protein that is critical for hemoglobin trafficking and drug sensitivity.
Article
Microbiology
Harpreet Kaur, Elisabeth Richardson, Komal Kamra, Joel B. Dacks
Summary: Protists in the phylum Ciliophora possess a complex membrane-trafficking system, including osmoregulatory Contractile Vacuoles and specialized secretory organelles. Molecular cell biological investigations in Tetrahymena thermophila have identified components of the protein machinery associated with the secretory organelles. Comparative genomics and phylogenetics studies have revealed the evolutionary history of the SM and Qa-SNARE proteins across the Ciliophora, showing independent expansions of these protein families in several ciliate classes.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2022)
Article
Microbiology
Yasinee Phanprasert, Kacper Maciszewski, Eleni Gentekaki, Joel B. Dacks
Summary: The chlorophyte algae, a dominant group of photosynthetic eukaryotes, exhibit diverse physical characteristics and nutritional modes. Our study reveals the conservation of MTCs across green algae, but also highlights taxon-specific losses, suggesting unusual endomembrane systems.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2023)
Article
Cell Biology
Aspasia Kontou, Emily K. Herman, Mark C. Field, Joel B. Dacks, V. Lila Koumandou
Summary: The endoplasmic reticulum (ER) is the largest intracellular organelle and plays a crucial role in protein synthesis and cellular function. Through comparative genomics analysis, it has been found that many proteins involved in maintaining ER structure are highly conserved and have an origin predating the last eukaryotic common ancestor. However, there are also lineage-specific innovations and alternative strategies for ER maintenance and elaboration in modern eukaryotes.
Article
Microbiology
Martin Zoltner, Mark C. Field
Summary: Euglena gracilis is a robust photosynthetic flagellate with a large nuclear genome and multiple endosymbiotic/gene transfer events. Its extraordinary metabolic plasticity and mixotrophic lifestyle enable it to thrive in diverse environments and produce novel metabolites.
Correction
Cell Biology
Aspasia Kontou, Emily K. Herman, Mark C. Field, Joel B. Dacks, V. Lila Koumandou
Review
Parasitology
Julius Lukes, Ambar Kachale, Jan Votypka, Anzhelika Butenko, Mark C. Field
Summary: This article critically re-evaluates the naming convention for Trypanosoma brucei parasites, proposing the use of ecotypes as a more accurate and appropriate designation.
TRENDS IN PARASITOLOGY
(2022)
Article
Cell Biology
Paul T. Manna, Lael D. Barlow, Inmaculada Ramirez-Macias, Emily K. Herman, Joel B. Dacks
Summary: This study used a transcriptomics-based approach to identify gene products associated with contractile vacuoles (CVs) in Dictyostelium discoideum. The approach was validated by confirming the upregulation of known CV-associated gene products and finding enrichment of endosomal and vacuolar gene products. An upregulated SNARE protein (NPSNB) was found to be associated with CVs, suggesting its role in CV function. These findings provide important insights into the deep evolution of eukaryotic organelles.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Galina Prokopchuk, Anzhelika Butenko, Joel B. Dacks, Dave Speijer, Mark C. Field, Julius Lukes
Summary: Genetic variation is crucial for adaptation and evolution, particularly in terms of changes in protein complex composition and subunit sequence. Comparative genomics can reveal some of these changes, but in silico analysis alone is insufficient. Combining data from both computational and experimental approaches, this study explores the range of protein complex compositional changes during evolution, using trypanosomes as a well-studied example. The findings highlight the importance of gene loss in diversifying protein complexes and enhancing eukaryotic diversity.
BIOLOGICAL REVIEWS
(2023)
Article
Immunology
Congshan Liu, Jianping Cao, Haobing Zhang, Mark C. Field, Jianhai Yin
Summary: The study suggests that tapeworm-secreted extracellular vesicles (EVs) may promote angiogenesis in Echinococcus infections, revealing central mechanisms of Echinococcus-host interactions.
MICROBES AND INFECTION
(2023)
Article
Biochemistry & Molecular Biology
Drahomira Faktorova, Kristina Zahonova, Corinna Benz, Joel B. Dacks, Mark C. Field, Julius Lukes
Summary: Sec13 plays multiple roles in different cellular processes as a component of COPII endoplasmic reticulum export vesicle coat, nuclear pore complex (NPC), and Seh1-associated (SEA)/GATOR nutrient-sensing complex. Euglenozoa possesses two Sec13 paralogues, with Sec13a interacting with COPII and NPC, and Sec13b interacting with Sec16 and SEA/GATOR complex. This suggests a distinct organization of coatomer complexes in euglenozoan flagellates.
Review
Parasitology
Julius Lukes, Dave Speijer, Alena Zikova, Juan D. Alfonzo, Hassan Hashimi, Mark C. Field
Summary: The African trypanosome, Trypanosoma brucei, is an important experimental model for molecular and cellular parasitology, contributing to the understanding of diverse biological features and pushing the boundaries of cell biology.
TRENDS IN PARASITOLOGY
(2023)
Article
Cell Biology
Mark C. Field
Summary: The nucleus is highly conserved, but there are significant compositional divergences within nuclear structures among different eukaryotic lineages, reflecting adaptations and distinct mechanisms. These studies highlight the malleability of nuclear architecture and call for further exploration of nuclear function in non-canonical experimental organisms.
CURRENT OPINION IN CELL BIOLOGY
(2023)
Article
Microbiology
Angana Mukherjee, Marie-Eve Crochetiere, Audrey Sergerie, Souad Amiar, L. Alexa Thompson, Zeinab Ebrahimzadeh, Dominic Gagnon, Florian Lauruol, Alexandra Bourgeois, Thomas Galaup, Stephanie Roucheray, Stephanie Hallee, Prasad K. Padmanabhan, Robert Stahelin, Joel B. Dacks, Dave Richard
Summary: Our study attempted gene inactivation of 24 potential phosphoinositide metabolism effectors in Plasmodium falciparum, revealing that 79% of candidates are likely essential for parasite growth. We identified PfPX1, a phosphoinositide-binding protein critical for hemoglobin trafficking, and its inactivation resulted in parasite artemisinin resistance. The findings support the potential of targeting the phosphoinositide pathway for antimalarial drug development.