Article
Cell Biology
Nicolas Boehly, Ann-Kathrin Schmidt, Xiaoxiao Zhang, Benjamin O. Slusarenko, Magdalena Hennecke, Maik Kschischo, Holger Bastians
Summary: Replication stress-induced increased origin firing can trigger chromosomal instability in human cancer cells, which is associated with W-CIN in human cancer specimens.
Article
Cell Biology
Tobias Strunz, Martin Kellner, Christina Kiel, Bernhard H. F. Weber
Summary: This study identified clusters of co-regulated genes across multiple tissues using eQTL analysis and colocalization studies, highlighting the importance of understanding common effects on biological pathways and treatment implications when targeting co-regulated disease genes.
Article
Cell & Tissue Engineering
Yuqing Zhu, Chen Cheng, Lang Chen, Li Zhang, Hongru Pan, Linxiao Hou, Zhen Sun, Ling Zhang, Xudong Fu, Kuan Yoow Chan, Jin Zhang
Summary: Mouse embryonic stem cells exhibit cell-to-cell heterogeneity, with a small number of two-cell-like cells spontaneously emerging and transitioning back to a pluripotent state without external stimuli. Single-cell RNA sequencing revealed a step-by-step transitional process between the two-cell-like and pluripotent states, with the cell cycle playing a key role in regulating these transitions by influencing the expression of 2C gene Dux.
Article
Biochemistry & Molecular Biology
Mitsuru Okuwaki, Shoko Saito, Hiroko Hirawake-Mogi, Kyosuke Nagata
Summary: This study revealed that the nucleolar localization of NPM1 and the large ribosomal subunit precursors are mutually dependent. NPM1 plays a crucial role in maintaining the structure of large ribosomal subunits and the accumulation of late processing machinery in the nucleolus.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Microbiology
Juergen Tomasch, Sonja Koppenhofer, Andrew S. Lang
Summary: The study investigated the conservation of CtrA regulatory network genes in alphaproteobacteria, revealing differential positioning influenced by selection pressure in orders such as Rhodobacterales, Caulobacterales, and Rhizobiales.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mason A. McCool, Amber F. Buhagiar, Carson J. Bryant, Lisa M. Ogawa, Laura Abriola, Yulia V. Surovtseva, Susan J. Baserga
Summary: This study shows that the nucleolar protein RSL24D1 and the PeBoW complex play important roles in multiple steps of ribosome biogenesis, including pre-ribosomal RNA transcription and mature 28S rRNA production. They regulate pre-ribosomal RNA transcription by modulating the steady-state levels of RNA polymerase I. Specifically, RSL24D1 is demonstrated to coimmunoprecipitate with the RNA polymerase I subunit, RPA194, and together regulate the process of ribosome biogenesis.
Article
Biochemistry & Molecular Biology
Leonid Anikin, Dimitri G. Pestov
Summary: Aminoacridines, historically used as antiseptic and antiparasitic agents, could be repurposed for therapeutic use and new drug development. In this study, the effects of 9-aminoacridine on pre-rRNA metabolism in mammalian cells were investigated. The results showed that 9-aminoacridine inhibits both pre-rRNA transcription and processing, leading to the disruption of ribosome biogenesis. The ability of 9-aminoacridine to bind to RNA in vitro suggests its potential as a new ribosome biogenesis inhibitor.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Monika Zakrzewska-Placzek, Anna Golisz-Mocydlarz, Michal Krzyszton, Justyna Piotrowska, Malgorzata Lichocka, Joanna Kufel
Summary: This study demonstrates the involvement of AtNOL12 in rRNA biogenesis and its correlation with stress response in plants. The absence of AtNOL12 alters the expression of ribosomal protein and ribosome biogenesis genes, potentially impacting ribosome function.
Review
Microbiology
Liting Shao, Weiran Shen, Shengqi Wang, Jianming Qiu
Summary: Human bocavirus 1 (HBoV1) is an autonomous human parvovirus that causes acute respiratory tract infections in children and infects human airway epithelial cells in vitro. It expresses multiple proteins and RNA, playing important roles in viral gene expression and replication, and serving as a helper virus for other viruses in human cells.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Ruiming Li, Chun-Yu Lin, Wei-Feng Guo, Tatsuya Akutsu
Summary: The weighted MFVS (WMFVS) method integrates gene differential expression values with MFVS to select the maximum-weighted MFVS from all possible MFVSs, achieving better performance than using traditional bio-data or network-data analyses alone. This method balances the advantages of differential gene expression analyses and network analyses, improving accuracy and decreasing instability in the prediction of cancer genes. WMFVS can be easily applied to various kinds of networks, providing a useful framework for data analysis and prediction.
BMC BIOINFORMATICS
(2021)
Article
Neurosciences
Roderick T. Hori, Mohammad Moshahid Khan, Jianfeng Xiao, Phillip W. Hargrove, Tom Moss, Mark S. LeDoux
Summary: The UBTF E210K neuroregression syndrome is caused by mutations in the UBTF gene, leading to negative effects on neural function in humans. Through mouse experiments, it was discovered that reduced or silenced Ubtf gene resulted in abnormal behavior, cognition, motor skills, and molecular level changes, suggesting that loss-of-function mechanisms may play a role in humans as well.
Article
Plant Sciences
Yixiu Yao, Li Jia, Yuan Cheng, Meiying Ruan, Qingjing Ye, Rongqing Wang, Zhuping Yao, Guozhi Zhou, Jia Liu, Jiahong Yu, Peng Zhang, Yuhe Yin, Weiping Diao, Hongjian Wan
Summary: This study identified and analyzed the members of the CCO gene family in pepper and other plants using bioinformatics methods. A total of 158 CCO genes were found, which were further divided into two groups. Results from RNA-seq and qRT-PCR experiments showed that CaCCO genes in pepper responded to H2O2 and other abiotic stresses, indicating their important roles in plant stress responses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jianguo Wang, Sidi Ma, Peijie Yu, Xionglei He
Summary: The human brain exhibits anatomical symmetry, but also possesses fine-scale structural asymmetries that contribute to distinct functional divisions. Previous studies suggested that these asymmetric structures are primate specific or unique to humans, indicating recent evolution of genes responsible for human brain asymmetry. In our study, we identified over 1,500 traits associated with human brain asymmetry and discovered approximately 200 genes that are predominantly upregulated in brain tissues. Surprisingly, these genes are evolutionarily ancient and show brain-specific upregulation in humans, indicating the role of ancient genes in shaping the structural asymmetry of the human brain over time.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Parasitology
Sandor Hornok, Jeno Kontschan, Nora Takacs, Heloise Heyne, Aron Botond Kovacs, Olivier Plantard, Gergo Keve, Denis Fedorov, Miklos Gyuranecz, Ali Halajian
Summary: The genus Ixodes has the highest number of species among hard ticks, and the majority of them are found in the Afrotropical region. This study analyzed 58 Ixodes spp. ticks collected from mammalian and avian hosts in South Africa using molecular and phylogenetic methods. The results identified 11 morphologically distinct tick species, with the majority belonging to the Ixodes pilosus group. Molecular analyses confirmed the presence of two species in this group and discovered a new species closely related to I. rubicundus. Phylogenetic analyses also revealed the relationships between different subgenera of Ixodes ticks.
PARASITES & VECTORS
(2023)
Article
Biochemistry & Molecular Biology
Christian Sudfeld, Ana Pozo-Rodriguez, Sara A. Manjavacas Diez, Rene H. Wijffels, Maria J. Barbosa, Sarah D'Adamo
Summary: This study reports a novel gene expression system discovered in the oleaginous microalga Nannochloropsis, utilizing the highly efficient transcriptional activity of RNA polymerase I and an internal ribosome entry site for translation. By identifying the nucleolus as a genomic safe harbor for Pol I transcription and constructing transformant strains, consistently strong transgene expression was achieved, providing a powerful tool for genetic and metabolic engineering of microalgae and potential significant contributions to microalgal research.