Article
Cell Biology
Kristina Mitic, Marianne Grafe, Petros Batsios, Irene Meyer
Summary: Dictyostelium cells undergo a semi-closed mitosis, during which the nuclear envelope persists but free diffusion occurs between the cytoplasm and nucleus. Partial disassembly of the nuclear pore complexes allows for permeabilization of the nuclear envelope and facilitates the formation of the mitotic spindle.
Article
Microbiology
Sushma Ambekar, Josh R. Beck, Gunnar R. Mair
Summary: The nuclear pore complex (NPC) is a platform for studying the evolution of early-branching eukaryotes, but the Plasmodium NPC is poorly defined due to its evolutionary divergence. In this study, a labeling methodology based on TurboID fusion proteins was developed to visualize and identify the components of the Plasmodium NPC. Through affinity purification and mass spectrometry, several known nucleoporins (Nups) and previously unannotated proteins were identified as components of the NPC. These findings contribute to a better understanding of the function and evolutionary position of the Plasmodium NPC.
Review
Biochemistry & Molecular Biology
America Chandia Cristi, Sampath Rapuri, Alyssa N. Coyne
Summary: Nuclear pore complexes (NPCs) are crucial for maintaining nuclear-cytoplasmic balance and proper nuclear organization, and perturbations in their regulation are implicated in neurodegenerative diseases. The ESCRT-III pathway plays a critical role in the surveillance and preservation of functional NPCs and nuclear envelope sealing. Maintaining quality control of the nuclear envelope and NPCs is pivotal in the pathogenesis of neurodegenerative diseases.
Article
Biochemistry & Molecular Biology
Murray Stewart
Summary: Although the nuclear envelope separates the nucleus and cytoplasm in eukaryotes, a semipermeable barrier is necessary to allow material exchange between these compartments. This barrier is achieved through nuclear pores, which facilitate the active transport of macromolecules. The diffusion of macromolecules through these pores is impeded by a dense network of unfolded regions of nucleoporins. Additional mechanisms, such as binding to immobile components and active removal, are required to maintain the distinctive compositions of each compartment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Arantxa Agote-Aran, Junyan Lin, Izabela Sumara
Summary: Nuclear pore complexes are crucial for macromolecular transport between the nucleus and the cytoplasm. Fragile X-related proteins play a role in the localization of nucleoporins, affecting NPC function. The clearance of cytoplasmic nucleoporin granules is independent of protein translation and is restricted to early G1 phase of the cell cycle.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wanlu Zhang, Azqa Khan, Jlenia Vitale, Annett Neuner, Kerstin Rink, Christian Luechtenborg, Britta Bruegger, Thomas H. Soellner, Elmar Schiebel
Summary: The short amphipathic alpha-helix of Apq12 regulates the function of Brl1 and Brr6, leading to defects in NPC biogenesis and NE integrity when disrupted. Overexpression of Apq12 promotes over-proliferation of the ONM/ER and accumulation of phosphatidic acid at the NE, possibly during NPC biogenesis.
Review
Plant Sciences
Daniel Ludke, Philipp F. W. Rohmann, Marcel Wiermer
Summary: Plant viability and responses to changing environments depend on spatial communication between the compartments separated by the nuclear envelope, involving bidirectional exchange of proteins and RNAs mediated by a sophisticated transport machinery.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Hideharu Hashimoto, Daniel H. Ramirez, Ophelie Lautier, Natalie Pawlak, Gunter Blobel, Benoit Palancade, Erik W. Debler
Summary: The study reports the retention of unspliced pre-mRNA by ScPml39 protein in Saccharomyces cerevisiae prior to export through nuclear pore complexes. The crystal structure of ScPml39 was determined, revealing its structural orthologs and providing insights into its function and mechanism.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Yan Chen, Yuan Zhang, Xiangyu Zhou
Summary: Nucleoporins play important roles not only in nuclear pore complexes but also in the base of cilia, with non-classical functions that are linked to human diseases. Elucidating these non-classical roles of nucleoporins will enhance our understanding of ciliopathy etiology.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Oncology
Finn Becker, Anne Offermann, Marie C. Roesch, Vincent Joerg, Doris Roth, Verena Lubczyk, Rainer Kuefer, Verena Sailer, Jutta Kirfel, Axel S. Merseburger, Sven Perner
Summary: The study demonstrates the potential of POM121 as a prognostic biomarker for prostate cancer, with increasing expression levels during tumor progression and independent predictive value for biochemical recurrence-free survival (BFS) regardless of other clinicopathological markers.
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2022)
Article
Parasitology
Ruisha Shang, Feng Zhu, Yu Li, Ping He, Jingru Qi, Yong Chen, Fuzhen Sun, Yiling Zhang, Qiang Wang, Zhongyuan Shen
Summary: This study identified a NbNup170 protein in microsporidian Nosema bombycis for the first time and found its specific localization pattern within the cell. The results suggest that NbNup170 plays a crucial role in the proliferation and sporulation phases of N. bombycis.
PARASITOLOGY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Julie Jacquemyn, Joyce Foroozandeh, Katlijn Vints, Jef Swerts, Patrik Verstreken, Natalia Gounko, Sandra F. Gallego, Rose Goodchild
Summary: Torsin affects lipid metabolism by regulating NEP1R1-CTDNEP1 and Lipin, influencing nuclear envelope remodeling and nuclear pore complex biogenesis. This mechanism explains some of the pleiotropic effects of Torsins.
Article
Neurosciences
Alyssa N. Coyne, Jeffrey D. Rothstein
Summary: Nuclear pore complex injury plays a significant role in ALS pathogenesis, with aberrant nuclear accumulation of the ESCRT-III protein CHMP7 being a key pathological event. While VPS4 expression is increased in ALS neuronal nuclei, CHMP4B and CHMP2B levels do not show a similar increase. Therapeutic efforts to mitigate this pathogenic cascade should focus on upstream events such as the nuclear accumulation of CHMP7.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Tiantian Zheng, Anton Zilman
Summary: The nuclear pore complexes (NPCs) in eukaryotic cells are responsible for macromolecular transport in and out of the nucleus. The NPCs contain intrinsically disordered proteins (IDPs) and efficiently transport cargoes in crowded conditions. A computational model of the NPC shows that self-regulating mechanisms and the spatial architecture of the IDP assembly contribute to efficient transport.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Eva Hegedusova, Veronika Marsalova, Sneha Kulkarni, Zdenek Paris
Summary: The role of nucleoporins in tRNA export in Trypanosoma brucei has been investigated. It was found that TbNup62 and TbNup53a are involved in tRNA export, while TbNup144 plays a critical role in nuclear division. These findings suggest that the location of nucleoporins within the nuclear pore complex is crucial for various cellular processes.