Article
Cell Biology
Stephanie M. Stanford, Meghan Collins, Michael A. Diaz, Zachary J. Holmes, Paul Gries, Matthew R. Bliss, Alessia Lodi, Vida Zhang, Stefano Tiziani, Nunzio Bottini
Summary: LMPTP plays a key role in adipocyte differentiation by regulating PDGFRα signaling and affecting multiple pathways involved in fat cell growth, differentiation, and glucose utilization in adipose tissue.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ana Alonso-Perez, Maria Guillan-Fresco, Eloi Franco-Trepat, Alberto Jorge-Mora, Miriam Lopez-Fagundez, Andres Pazos-Perez, Antia Crespo-Golmar, Jose R. Caeiro-Rey, Rodolfo Gomez
Summary: The balance between adipogenesis and osteoblastogenesis is relevant in multiple diseases. Current in vitro differentiation models for human mesenchymal stem cells (hMSCs) are expensive and hardly reproducible, making it difficult to study the equilibrium between adipocytes and osteoblasts in an affordable and reliable manner. In this study, lab-made media were compared with consensus and commercial media, and a cell-line (C3H10T1/2) was identified to simultaneously evaluate both MSCs differentiations. Lab-made media significantly increased the differentiation of hMSCs into adipocytes and osteoblasts. Additionally, the lab-made media promoted adipocyte gene-markers expression in C3H10T1/2 and hMSC-TERT and osteoblast gene-markers in C3H10T1/2. The optimized lab-made media proved to be better at differentiating MSCs compared to consensus/commercial media and demonstrated the adipogenic commitment of pre-osteoblast-like cells from aged-women. C3H10T1/2 is an ideal MSC line for studying the balance between adipocytes and osteoblasts.
Article
Cell Biology
Rodrigo P. F. Abuna, Luciana O. Almeida, Alann T. P. Souza, Roger R. Fernandes, Thales F. V. Sverzut, Adalberto L. Rosa, Marcio M. Beloti
Summary: The study examined the impact of osteoporosis on osteoblast differentiation, revealing that osteoblasts are negatively affected when interacting with adipocytes, and the involvement of AcH3 in bone homeostasis. The findings demonstrated an unknown mechanism relevant to osteoporotic bone loss, suggesting AcH3 as a potential target for future epigenetic-based therapies for bone diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
D. Masnikov, I Stafeev, S. Michurina, E. Zubkova, E. Mamontova, E. Ratner, M. Menshikov, Ye Parfyonova
Summary: The study revealed that the immortalized cell line ASC52Telo derived from human ADSC has a mesenchymal stem cell phenotype. However, the adipocytes derived from ASC52Telo exhibit impaired adipogenesis and insulin sensitivity compared to those derived from ADSC, which are important properties of healthy adipocytes.
ANALYTICAL BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Sasithon Senamontree, Thitiporn Lakthan, Pornsri Charoenpanich, Chanpen Chanchao, Adisri Charoenpanich
Summary: Betulinic acid can enhance osteogenesis and reduce adipogenesis in human mesenchymal stem cells, as well as promote brown adipocyte characteristics. These mechanisms improve glucose and lipid metabolism without potential side effects on bone loss or weight gain.
Article
Pharmacology & Pharmacy
M. S. M. Ardawi, M. H. Badawoud, S. M. Hassan, A. M. S. Ardawi, A. A. Rouzi, M. H. Qari, S. A. Mousa
Summary: Lycopene nanoparticles promote osteoblast differentiation in osteogenic medium but inhibit adipocyte differentiation in adipogenic medium.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2021)
Article
Food Science & Technology
Qi Zhang, Sijing Hu, Jianjun Wu, Peng Sun, Quanlong Zhang, Yang Wang, Qiming Zhao, Ting Han, Luping Qin, Qiaoyan Zhang
Summary: The oligosaccharide NST derived from Morinda officinalis demonstrated potential in preventing bone loss and promoting osteogenic differentiation of BMSCs, providing a potential alternative medicine for the treatment of osteoporosis.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Review
Pharmacology & Pharmacy
Christopher D. Kassotis, Frederick S. vom Saal, Patrick J. Babin, Dominique Lagadic-Gossmann, Helene Le Mentec, Bruce Blumberg, Nicole Mohajer, Antoine Legrand, Vesna Munic Kos, Corinne Martin-Chouly, Normand Podechard, Sophie Langoue, Charbel Touma, Robert Barouki, Min Ji Kim, Karine Audouze, Mahua Choudhury, Nitya Shree, Amita Bansal, Sarah Howard, Jerrold J. Heindel
Summary: There is increasing evidence that environmental contaminants can disrupt metabolic health in both humans and animals. In vitro models have been relied upon for evaluating adipogenic and obesogenic contaminants, but there is a need for more well-understood and properly managed models. Recent advancements in in vitro models, including human mesenchymal stem cell and preadipocyte models, show promise in improving translation to human metabolic health. Additionally, alternative model organisms like Drosophila, C. elegans, zebrafish, and medaka have been identified as having potential in metabolic health testing.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Jae Hwan Cho, Jae Hyup Lee, Kyung Mee Lee, Choon-Ki Lee, Dong-Myung Shin
Summary: This study compared the effects of rhBMP-2 on osteogenic and adipogenic differentiation of senescent and non-senescent MSCs, with findings showing a lesser induction of osteogenic differentiation in senescent MSCs by rhBMP-2. The study also highlighted different gene expression and signaling pathways between the two groups of cells, with non-senescent MSCs showing dominance in bone forming pathways.
CALCIFIED TISSUE INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Gang Wang, Lifu Wan, Lecheng Zhang, Chao Yan, Yuelei Zhang
Summary: This study found that miR-133a plays a role in regulating cell proliferation, osteoblast differentiation, and adipocyte differentiation of BMSCs by targeting FGFR1. BMSC-specific knockdown of miR-133a can attenuate bone loss.
DNA AND CELL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Tung-Shing Mamie Lih, Mingming Dong, Leslie Mangold, Alan Partin, Hui Zhang
Summary: This study investigated the clinical utility of urinary marker panels composed of urinary glycopeptides and/or urinary prostate-specific antigen (PSA) for distinguishing non-aggressive from aggressive prostate cancer. The multi-marker panels showed clinically meaningful results with area under the curve (AUC) ranging from 0.70 to 0.85.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: MSCs are a heterogeneous cell population found in various tissues in the body, with important regulatory functions, especially in the bone marrow where they play a critical role in regulating HSCs. They contribute to maintaining bone integrity and hormonal nutrition supply, as well as regulating the homeostasis and differentiation of the HSC niche through differentiation into different cell types. Research on MSCs helps understand their role in regulating cell interactions and homeostasis in the body.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Kevin Woods, Borhane Guezguez
Summary: Mesenchymal stromal cells (MSCs) are a diverse cell population known for their nutrient-producing and immunomodulatory functions, crucial in regulating hematopoietic stem cells (HSC) in the bone marrow for blood production and immunity. MSCs contribute to bone integrity and hormone supply by differentiating into osteoblasts and adipocytes, playing a key role in the complex interactions of the HSC niche.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Medicinal
Seung Tae Im, Hyun-Soo Kim, Won-Kyo Jung, Seung-Hong Lee
Summary: This study found that Ishophloroglucin A (IPA) has a high inhibitory activity against protein tyrosine phosphatase 1B (PTP1B) and can attenuate adipogenesis. The molecular docking analysis revealed a stable binding affinity between IPA and PTP1B, suggesting their interaction. These findings suggest that IPA may be a promising natural compound for the treatment of obesity and related diseases.
Article
Medicine, Research & Experimental
Jae Yeon Kim, Se Ho Kim, Jin Seok, Si Hyun Bae, Seong-Gyu Hwang, Gi Jin Kim
Summary: This study aimed to investigate the therapeutic effects of genetically modified bone marrow mesenchymal stem cells (BM-MSCs) overexpressing PRL-1 in a bile duct ligation (BDL)-injured cholestatic rat model. The results showed that BM-MSCsPRL-1 generated by nonviral gene delivery system improved antioxidant capacity, mitochondrial dynamics, and mitochondrial anaerobic metabolism, thus restoring hepatic function and exerting antifibrotic effects.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Review
Pharmacology & Pharmacy
Jinho Seo, Min Wook Kim, Kwang-Hee Bae, Sang Chul Lee, Jaewhan Song, Eun-Woo Lee
BIOCHEMICAL PHARMACOLOGY
(2019)
Article
Pharmacology & Pharmacy
Young-Lai Cho, Jong-Gil Park, Hyo Jin Kang, Wooil Kim, Min Ji Cho, Ju-Hong Jang, Min-Gi Kwon, Sungsik Kim, Sang-Hyun Lee, Jangwook Lee, Yeon-Gu Kim, Young-Jun Park, Won Kon Kim, Kwang-Hee Bae, Byoung-Mog Kwon, Sang J. Chung, Jeong-Ki Min
PHARMACOLOGICAL RESEARCH
(2019)
Article
Cell Biology
Jeong Su Park, Da Hyun Lee, Yu Seol Lee, Eunji Oh, Kwang-Hee Bae, Kyoung-Jin Oh, Hyunmi Kim, Soo Han Bae
Article
Biotechnology & Applied Microbiology
Kyungmin Lee, Jangwook Lee, Minjeong Kwak, Young-Lai Cho, Byungtae Hwang, Min Ji Cho, Na Geum Lee, Jongjin Park, Sang-Hyun Lee, Jong-Gil Park, Yeon-Gu Kim, Jang-Seong Kim, Tae-Su Han, Hyun-Soo Cho, Young-Jun Park, Seon-Jin Lee, Hee Gu Lee, Won Kon Kim, In Cheul Jeung, Nam Woong Song, Kwang-Hee Bae, Jeong-Ki Min
JOURNAL OF NANOBIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Ji Soo Kim, Won Kon Kim, Kyoung-Jin Oh, Eun-Woo Lee, Baek Soo Han, Sang Chul Lee, Kwang-Hee Bae
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Andrology
Seong-Ho Yang, San-Hyun Yoon, Jae-Noon Jung, Jin-Ho Lim, Yong Ko
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2019)
Editorial Material
Medicine, General & Internal
Won Kon Kim, Kwang-Hee Bae, Sang Chul Lee, Kyoung-Jin Oh
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Cell Biology
Jeong Yoon, Kyoung-Mi Juhn, Eun-Hye Jung, Hye-Jeong Park, San-Hyun Yoon, Yong Ko, Chang-Young Hur, Jin-Ho Lim
Article
Obstetrics & Gynecology
Seong-Ho Yang, Yong-Soo Hur, San-Hyun Yoon, Jae-Hoon Jung, Jin-Ho Lim, Yong Ko
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
(2020)
Review
Biology
Ji-Yoon Lee, Won Kon Kim, Kwang-Hee Bae, Sang Chul Lee, Eun-Woo Lee
Summary: Ferroptosis is a type of iron-dependent regulated necrosis induced by lipid peroxidation in cellular membranes. Polyunsaturated fatty acids associated with specific phospholipids are responsible for ferroptosis-inducing lipid peroxidation. Cells take up essential fatty acids from blood to produce PUFAs, which undergo lipid peroxidation through various pathways tightly regulated by metabolic and signaling pathways.
Article
Surgery
Min Ho Kim, Changho Chung, Mun Ho Oh, Jin Hyun Jun, Yong Ko, Jong Hun Lee
Summary: PVC-3D-EV significantly improved wound contraction, myofibroblast activation, collagen deposition, and angiogenesis, indicating its pivotal role in regulating granulation tissue formation, angiogenesis, and wound contraction in healing of a rat skin wound. The 3D-culture system using a polystyrene scaffold is shown to provide better physiological conditions than the 2D-culture system, and EVs from PVC cultured in 3D could be a promising option for healing skin wounds.
AESTHETIC PLASTIC SURGERY
(2021)
Review
Biochemistry & Molecular Biology
Hyunmi Kim, Da Som Lee, Tae Hyeon An, Hyun-Ju Park, Won Kon Kim, Kwang-Hee Bae, Kyoung-Jin Oh
Summary: Liver disease encompasses a spectrum of liver damage from simple steatosis to hepatocellular carcinoma, with nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes often coexisting. Despite the lack of suitable drugs for NAFLD and NASH, thiazolidinediones drugs are sometimes used to improve liver failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anna Park, Mihee Oh, Su Jeong Lee, Kyoung-Jin Oh, Eun-Woo Lee, Sang Chul Lee, Kwang-Hee Bae, Baek Soo Han, Won Kon Kim
Summary: Mitochondria are vital for cell survival and play essential roles in various cellular functions. Dysfunction in mitochondria is associated with multiple diseases and mitochondrial transplantation is considered a promising therapeutic approach in treating mitochondrial disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
DongHyun Shon, SeJun Park, SukJun Yoon, Yong Ko
Summary: This study investigated the developmental characteristics and classification of adipocytes using Fourier transform infrared (FTIR) spectroscopy. The results showed differences in secretory protein profiles and lipid amounts between white and brown adipocytes. Additionally, the study explored the possibility of using FTIR spectroscopy as a non-tagging cell sorting system.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Se-Jun Park, Dong-Hyun Shon, Jae-Hyun Kim, Yang-Hwan Ryu, Yong Ko
Summary: SAMM50 plays a key role in regulating thermogenesis of brown/beige adipocytes by modulating mitochondrial dynamics. Inhibition of SAMM50 suppresses the expression of thermogenic genes, while overexpression promotes their expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)