Article
Cell Biology
Chengye Feng, Joseph M. Cleary, Gregory O. Kothe, Michelle C. Stone, Alexis T. Weiner, James Hertzler, William O. Hancock, Melissa M. Rolls
Summary: Axons and dendrites are distinguished by microtubule polarity, with dendrites containing minus-end-out microtubules and axons having plus-end-out microtubules. Trim9 and kinesin-5 work together at microtubule plus ends to promote new microtubule growth and resist catastrophe. Successful exit of microtubules at branch points depends on their orientation and parallel orientation with other microtubules.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Saaya Hatakeyama, Akihiro Tojo, Hiroshi Satonaka, Nami O. Yamada, Takao Senda, Toshihiko Ishimitsu
Summary: In a study on mice lacking APC, it was found that podocyte vesicle transport was suppressed under induction of minimal change nephrotic syndrome, leading to reduced albuminuria compared to wild-type mice. This suggests a crucial role of APC in maintaining podocyte vesicle transport and renal function during nephrotic syndrome development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Daisuke Yamazaki, Osamu Hashizume, Shiho Taniguchi, Yosuke Funato, Hiroaki Miki
Summary: The study on APC gene using a three-dimensional organoid culture of mouse colon epithelia revealed its role in cell proliferation and differentiation regulation, as well as identified novel characteristics related to tumor development and ectopic differentiation. The findings highlight the importance of investigating molecular mechanisms and biological significance of ectopic differentiation in cancer development using colon organoids.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Celine Cuche, Marta Mastrogiovanni, Marie Juzans, Helene Laude, Marie-Noelle Ungeheuer, Daniel Krentzel, Maria Isabella Gariboldi, Daniel Scott-Algara, Marianne Madec, Sophie Goyard, Camille Floch, Gaelle Chauveau-Le Friec, Pierre Lafaye, Charlotte Renaudat, Muriel Le Bidan, Christine Micallef, Sandrine Schmutz, Sebastien Mella, Sophie Novault, Milena Hasan, Darragh Duffy, Vincenzo Di Bartolo, Andres Alcover
Summary: Familial adenomatous polyposis (FAP) is an inherited disease characterized by the development of colorectal adenomas with high risk of becoming colorectal tumors. Mutations of the Adenomatous polyposis coli (APC) gene are often responsible for FAP. Our recent study suggests that the APC protein is involved in multiple phases of T cell responses. We investigated immune cell abnormalities in FAP subjects and found dysfunctions in various immune cell populations, gene expressions, cytokine and chemokine productions, and T cell migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Genetics & Heredity
Martin R. Graf, Shruti Apte, Esteban Terzo, Simran Padhye, Shuhao Shi, Megan K. Cox, Roger B. Clark, Vijay Modur, Vasudeo Badarinarayana
Summary: Familial adenomatous polyposis (FAP) is a colorectal disease caused by mutations in the APC gene, leading to the growth of adenomatous polyps. The novel macrolide, ZKN-0013, has been shown to restore the function of the APC protein and inhibit the beta-catenin/wnt-pathway in human colon carcinoma cells and a mouse model of FAP. Treatment with ZKN-0013 reduced the number of intestinal polyps, adenomas, anemia, and improved survival.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Developmental Biology
Melissa M. Rolls
Summary: The microtubule cytoskeleton is crucial for long-lived neurons, with their correct polarity being essential for transporting cargoes in the right direction. This review focuses on how microtubule polarity is established and maintained in neurons, highlighting the surprising complexity of mechanisms involved in organizing microtubules in the correct orientation.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Xingyuan Fang, Tatyana M. Svitkina
Summary: The APC protein, known for its role as a tumor suppressor and in Wnt signaling regulation, also plays a crucial role in cell migration as a cytoskeletal protein. Mutations in the APC gene are associated with colorectal cancer and neurological disorders. Understanding how APC regulates cell motility is still incomplete, but recent research suggests its involvement in initiating leading edge protrusion through interactions with microtubules and the Arp2/3 complex.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
John Gasdal Karstensen, Steffen Bulow, Helle Hojen, Anne Marie Jelsig, Niels Jespersen, Klaus Kaae Andersen, Mads Damsgaard Wewer, Johan Burisch, Hans Christian Pommergaard
Summary: This study found that patients with familial adenomatous polyposis (FAP) have a significantly higher risk of developing cancer compared to controls, mainly due to increased risks of colorectal, pancreatic, and duodenal/small-bowel cancers. Additionally, the risk of developing a second primary cancer is also significantly higher for FAP patients.
Article
Medicine, General & Internal
Maria Lourdes Garza-Rodriguez, Victor Trevino, Antonio Ali Perez-Maya, Hazyadee Frecia Rodriguez-Gutierrez, Moises Gonzalez-Escamilla, Miguel Angel Elizondo-Riojas, Genaro A. Ramirez-Correa, Oscar Vidal-Gutierrez, Carlos Horacio Burciaga-Flores, Diana Cristina Perez-Ibave
Summary: In this study, a novel pathogenic germline variant in the APC gene was identified using NGS. The variant was confirmed in multiple family members and associated with atypical clinical symptoms. This variant is classified as a PVS1 variant according to ACMG guidelines, providing evidence for early surveillance and suitable treatment in patients with the variant.
Article
Cell Biology
Helena Rannikmae, Samantha Peel, Simon Barry, Takao Senda, Marc de la Roche
Summary: The Apc protein regulates key effector pathways for tissue homeostasis, with truncating mutations leading to intestinal epithelial tumorigenesis by affecting Wnt pathway and microtubule dynamics. While Wnt pathway plays a role in cell proliferation, Apc also controls epithelial morphology independently of Wnt-responsive Myc-335 element, and disrupts microtubule cytoskeleton without affecting actin cytoskeleton distribution. This study identifies three independent Apc effector pathways in intestinal epithelial regulation: proliferation, microtubule dynamics, and epithelial morphology.
JOURNAL OF CELL SCIENCE
(2021)
Article
Surgery
Jin Hee Noh, Eun Mi Song, Ji Yong Ahn, Dong-Hoon Yang, Woochang Lee, Jinyoung Hong, Aram Kim, Hee Kyong Na, Jeong Hoon Lee, Kee Wook Jung, Do Hoon Kim, Kee Don Choi, Ho June Song, Gin Hyug Lee, Hwoon-Yong Jung
Summary: Upper GI neoplasms are not rare in patients with familial adenomatous polyposis (FAP), with more than half of the patients having gastric adenoma, gastric cancer, nonampullary duodenal adenoma, or ampullary adenoma. Endoscopic treatment has shown positive outcomes in treating these neoplasms, reducing the severity of duodenal polyposis and improving long-term prognosis.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Review
Cell Biology
James Abbott, Inke S. Nathke
Summary: Mutations in the APC gene have been found to be driver mutations in colorectal cancers, and the importance of APC in normal tissue homeostasis has been confirmed in various organisms. APC has multiple functions, including acting as a scaffold protein in signaling pathways and regulating the cytoskeleton. Mutations in APC are strongly associated with colorectal cancers, particularly those resulting in truncated proteins. Understanding the diverse functions and interactions of APC requires knowledge of its structure and conservation across taxonomy.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Casey D. D. Stefanski, Anne Arnason, Sara Maloney, Janna Kotsen, Elizabeth Powers, Jian-Ting Zhang, Jenifer R. R. Prosperi
Summary: Chemoresistance is a major concern for cancer patients, and one mechanism is the increased expression of ABC transporters, which prevent drug accumulation in cells. Our lab found that the loss of APC gene leads to intrinsic resistance to the drug doxorubicin, potentially through enhanced tumor-initiating cell population and increased STAT3 activation. In mouse mammary tumor cells, the loss of APC decreased drug accumulation and increased protein levels of MDR1 and MRP1. However, the correlation between APC and ABC transporters was not significant in breast cancer patients or cell lines. Inhibitors of ABC transporters showed potential as therapeutic targets in APC-deficient tumors by reducing tumor-initiating cell population and increasing drug-induced apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Lautaro Baro, Asifa Islam, Hannah M. Brown, Zoe A. Bell, M. Angeles Juanes
Summary: Cell remodeling relies on dynamic rearrangements of cell contacts powered by the actin cytoskeleton. The tumor suppressor APC nucleate actin filaments and localize at cell junctions. The role of APC-driven actin nucleation in cell junction remodeling remains unknown.
Article
Oncology
Yoshiko Mori, Hideyuki Ishida, Noriyasu Chika, Tetsuya Ito, Kunihiko Amano, Kenichi Chikatani, Yoji Takeuchi, Mitsuhiro Kono, Satoki Shichijo, Akiko Chino, Toshiya Nagasaki, Akinari Takao, Misato Takao, Sakiko Nakamori, Kazuhito Sasaki, Kiwamu Akagi, Tatsuro Yamaguchi, Kohji Tanakaya, Tomita Naohiro, Yoichi Ajioka
Summary: Genotype-oriented management may be beneficial for the clinical management of patients with familial adenomatous polyposis (FAP). This strategy needs to be validated in further prospective studies focusing on long-term endoscopic intervention and optimal age at prophylactic (procto)colectomy.
INTERNATIONAL JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Developmental Biology
Catarina R. Oliveira, Regis Lemaitre, Prayag Murawala, Akira Tazaki, David N. Drechsel, Elly M. Tanaka
DEVELOPMENTAL BIOLOGY
(2018)
Article
Developmental Biology
Wouter Masselink, Daniel Reumann, Prayag Murawala, Pawel Pasierbek, Yuka Taniguchi, Francois Bonnay, Katharina Meixner, Juergen A. Knoblich, Elly M. Tanaka
Correction
Biochemical Research Methods
Ji-Feng Fei, Wilson Pak-Kin Lou, Dunja Knapp, Prayag Murawala, Tobias Gerber, Yuka Taniguchi, Sergej Nowoshilow, Shahryar Khattak, Elly M. Tanaka
Article
Biology
Vishal Dandewad, Arya Vindu, Jomon Joseph, Vasudevan Seshadri
JOURNAL OF BIOSCIENCES
(2019)
Article
Cell Biology
Indrasen Magre, Vikas Fandade, Indraneel Damle, Poulomi Banerjee, Santosh Kumar Yadav, Mahendra Sonawane, Jomon Joseph
JOURNAL OF CELL SCIENCE
(2019)
Article
Biology
Joshua D. Currie, Lidia Grosser, Prayag Murawala, Maritta Schuez, Martin Michel, Elly M. Tanaka, Tatiana Sandoval-Guzman
Article
Multidisciplinary Sciences
Marko Pende, Karim Vadiwala, Hannah Schmidbaur, Alexander W. Stockinger, Prayag Murawala, Saiedeh Saghafi, Marcus P. S. Dekens, Klaus Becker, Roger Revilla-I-Domingo, Sofia-Christina Papadopoulos, Martin Zurl, Pawel Pasierbek, Oleg Simakov, Elly M. Tanaka, Florian Raible, Hans-Ulrich Dodt
Article
Cell Biology
Tzi-Yang Lin, Tobias Gerber, Yuka Taniguchi-Sugiura, Prayag Murawala, Sarah Hermann, Lidia Grosser, Eri Shibata, Barbara Treutlein, Elly M. Tanaka
Summary: The difference in limb regeneration between salamanders and Xenopus laevis frogs lies in the dedifferentiation ability and integration of connective tissue cells, which restricts regeneration in frogs. Connective tissue cells in frog blastemas fail to fully re-express progenitor programs when transplanted into limb buds, affecting cartilage development. Additionally, there are molecular distinctions in cartilage differentiation programs between embryonic and adult frogs.
DEVELOPMENTAL CELL
(2021)
Review
Anatomy & Morphology
Lydia Tilley, Sofia-Christina Papadopoulos, Marko Pende, Ji-Feng Fei, Prayag Murawala
Summary: The ability to generate transgenic animals has led to a surge of research in various model organisms, yet the availability of transgenic lines in laboratory axolotls remains limited compared to other commonly used model organisms. This review discusses the history, advantages, and disadvantages of transgenic methodologies in axolotls, as well as insights gained from existing transgenic lines. Challenges in developing transgenic axolotls and the need for further research to enhance their role as developmental and regenerative models are also addressed.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Anatomy & Morphology
Sergej Nowoshilow, Ji-Feng Fei, S. Randal Voss, Elly M. Tanaka, Prayag Murawala
Summary: The laboratory axolotl (Ambystoma mexicanum) is widely used in biological research, particularly in tissue regeneration. Establishing gene and transgenic nomenclature has become a critical need to ensure uniformity in research, and guidelines for genetic nomenclature in working with axolotl have been proposed.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Prachi Deshmukh, Aditi Singh, Deepak Khuperkar, Jomon Joseph
Summary: Nup358 protein plays a role in coupling miRNA-induced silencing complex with target mRNA, and interacts with GW182 protein. Mutations in Nup358 associated with ANE1 may affect the miRNA pathway and contribute to the development of ANE1.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Prachi Deshmukh, Shubha Markande, Vikas Fandade, Yogendra Ramtirtha, Mallur Srivatsan Madhusudhan, Jomon Joseph
Summary: Micro-RNA mediated suppression of mRNA translation is a key regulatory mode of gene expression. Nup358-SIM has multiple interacting regions on AGO2, facilitating the coupling of miRISC with target mRNA. Specific residues are involved in the interaction of AGO2 with miRNA-loading components.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Genetics & Heredity
Rimpi Saikia, Jomon Joseph
Summary: Autophagy is a cell survival strategy that helps cells maintain homeostasis by degrading and reusing cellular components under unfavorable conditions. It is regulated by AMPK and mTORC1, with AMPK activating autophagy in energy-deprived conditions and mTORC1 inhibiting autophagy in nutrient-rich conditions. Additionally, cytoplasmic calcium can also activate AMPK and autophagy through a calmodulin/CaMKK beta pathway, although the physiological significance of this regulation is still unclear.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Cell Biology
Tzi-Yang Lin, Yuka Taniguchi-Sugiura, Prayag Murawala, Sarah Hermann, Elly M. Tanaka
Summary: Investigating cell lineage requires genetic tools that can label cells in a specific manner. This study developed a stable transgenic Xenopus line with a Cre-ERT2/loxP system for tracing fibroblasts during limb regeneration, providing the first demonstration of tissue-specific cell labeling with this system in Xenopus.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2022)
Article
Genetics & Heredity
Yan Hu, Xiangyu Pan, Yu Shi, Yuanhui Qiu, Liqun Wang, Prayag Murawala, Yanmei Liu, Wanjin Xing, Elly M. Tanaka, Ji-Feng Fei
Summary: Muscle is not essential for guiding other cell types but affects joint formation during limb regeneration in axolotls.
FRONTIERS IN GENETICS
(2022)