Article
Biochemistry & Molecular Biology
Enkai Li, Kolapo M. Ajuwon
Summary: This study elucidated the significant role of dynamin-dependent endocytosis in regulating claudin-3 and 4 during nutrient stress in intestinal epithelial cells. Short-term starvation-induced degradation of TJP was found to be lysosome dependent. Modulation of certain endocytic pathways may be a potential mechanism for preserving tight junction integrity and function.
Article
Plant Sciences
Maryam Nasr Esfahani, Komaki Inoue, Kien Huu Nguyen, Ha Duc Chu, Yasuko Watanabe, Asaka Kanatani, David J. Burritt, Keiichi Mochida, Lam-Son Phan Tran
Summary: The study compared the transcriptome changes in chickpea roots and leaves under -Pi/+NO3-, +Pi/-NO3-, and -Pi/-NO3- conditions. The results showed that -Pi/-NO3- treatment had a lesser effect on gene expression changes related to Pi and NO3- transport, signalling networks, lipid remodelling, nitrogen and Pi scavenging/remobilization/recycling, carbon metabolism, and hormone metabolism compared to -Pi/+NO3- or +Pi/-NO3- treatments. Nutrient imbalance was found to be a stronger stimulus for molecular reprogramming than overall deficiency.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Cell Biology
Vikramjit Lahiri, Shree Padma Metur, Zehan Hu, Xinxin Song, Muriel Mari, Wayne D. Hawkins, Janakraj Bhattarai, Elizabeth Delorme-Axford, Fulvio Reggiori, Daolin Tang, Joern Dengjel, Daniel J. Klionsky
Summary: This study reveals that subtle changes in nutrient availability can have a significant impact on autophagy flux through unknown post-transcriptional regulatory mechanisms affecting the expression of key autophagy-inducing kinase. The identification of two novel post-transcriptional regulators further highlights the complexity of autophagy regulation.
Article
Cell Biology
Aida Rodriguez Lopez, Maria H. Jorgensen, Jesper F. Havelund, Frederic S. Arendrup, Srinivasa Prasad Kolapalli, Thorbjorn M. Nielsen, Eva Pais, Carsten Jorn Beese, Ahmad Abdul-Al, Anna Constance Vind, Jiri Bartek, Simon Bekker-Jensen, Marta Montes, Panagiotis Galanos, Nils Faergeman, Lotta Happonen, Lisa B. Frankel
Summary: Ribosomes are selectively targeted for degradation by autophagy during cell senescence, contributing to alterations in cell metabolome and the senescence-associated secretory phenotype.
Editorial Material
Neurosciences
Olivia C. Davis, Theodore J. Price
Summary: Li et al. demonstrate that Tiam1 connects Rac1-mediated dendritic spine remodeling to NMDA receptor activity, leading to the promotion of behavioral signs of chronic pain in rodents.
Article
Chemistry, Multidisciplinary
Tianyi Wang, Yaming Zhang, Yuwei Liu, Yi Huang, Weiping Wang
Summary: This study demonstrates that under amino acid starvation, macropinocytosis and ubiquitin-proteasome system are activated as a nutrient supply route. Therefore, amino acid deprivation also sensitizes cancer cells to proteasome inhibition. By blocking macropinocytosis and proteasome simultaneously, an innovative approach is presented to exacerbate amino acid starvation in cancer therapy. A pH-responsive polymersome nanocarrier is developed to maximize therapeutic efficacy and minimize systemic side effects.
Article
Microbiology
Dou Wang, Yulin Wang, Lei Liu, Yiqiang Chen, Chunxiao Wang, Yu-You Li, Tong Zhang
Summary: The transcription patterns of anammox consortia changed significantly under nutrient starvation, indicating a starvation response mechanism. The core autotrophic producers of anammox bacteria were highly resilient and quickly recovered when feeding was resumed. These findings provide important insights into the capacity of anammox consortia to respond to stress and maintain process stability.
Article
Multidisciplinary Sciences
Wenyan Wan, Hui Dong, De-Hua Lai, Jiong Yang, Kai He, Xiaoyan Tang, Qun Liu, Geoff Hide, Xing-Quan Zhu, L. David Sibley, Zhao-Rong Lun, Shaojun Long
Summary: The micropore is an essential organelle for the endocytosis of nutrients from the host cell cytosol and Golgi in Toxoplasma gondii. It requires the ceramide de novo synthesis pathway in the parasite for optimal activity.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Markus Galhuber, Helene Michenthaler, Christoph Heininger, Isabel Reinisch, Christoph Noessing, Jelena Krstic, Nadja Kupper, Elisabeth Moyschewitz, Martina Auer, Ellen Heitzer, Peter Ulz, Ruth Birner-Gruenberger, Laura Liesinger, Georgia Ngawai Lenihan-Geels, Moritz Oster, Emil Spreitzer, Riccardo Zenezini Chiozzi, Tim J. Schulz, Michael Schupp, Tobias Madl, Albert J. R. Heck, Andreas Prokesch
Summary: This study reveals the mechanisms of p53 signaling during starvation using multiple methods. By utilizing BioID and immunoprecipitation techniques, the interaction network of p53 with metabolic enzymes and kinases was identified. Additionally, transcriptomics analysis identified a starvation-specific transcriptome response. These findings shed new light on our understanding of p53 as a nutrient stress sensor.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Plant Sciences
Misao Shimojo, Masashi Nakamura, Ginga Kitaura, Yuta Ihara, Shinsuke Shimizu, Koichi Hori, Masako Iwai, Hiroyuki Ohta, Kimitsune Ishizaki, Mie Shimojima
Summary: This study analyzed the impact of membrane lipid remodeling on inorganic phosphate uptake and discovered the crucial role of MpPAH in the growth of Marchantia polymorpha. The mutation of MpPAH affects plant growth by suppressing the synthesis of plastid glycolipids through the endoplasmic reticulum pathway.
Article
Microbiology
Nevena Maslac, Chandni Sidhu, Hanno Teeling, Tristan Wagner
Summary: Some marine thermophilic methanogens are able to perform energy-consuming nitrogen fixation despite deriving only little energy from hydrogenotrophic methanogenesis. Researchers studied the process in Methanothermococcus thermolithotrophicus DSM 2095 and found that diazotrophic growth is dependent on molybdenum and involves upregulation of nitrogenase machinery and other genes. This research provides insights into how these organisms adapt to nitrogen and energy limitations.
Article
Biochemistry & Molecular Biology
Sudeep Karki, Matti Javanainen, Shahid Rehan, Dale Tranter, Juho Kellosalo, Juha Huiskonen, Lotta Happonen, Ville Paavilainen
Summary: This study reveals the mechanism by which TRAP assists in polypeptide translocation by presenting the structure of the core Sec61/TRAP complex bound to a mammalian ribosome using cryo-EM.
Article
Oncology
Xiaoqing Wang, Yue Li, Yunyun Xiao, Xinjian Huang, Xianqiu Wu, Zhen Zhao, Muwen Yang, Lingzhi Kong, Dongni Shi, Xin Chen, Ying Ouyang, Xiangfu Chen, Chuyong Lin, Jun Li, Libing Song, Ye Lin, Jian Guan
Summary: Macropinocytosis is crucial for rapid proliferation of hepatocellular carcinoma cells under nutrient-limited conditions, and ATP9A plays a pivotal role in regulating this process. High ATP9A levels are associated with poor prognosis in hepatocellular carcinoma patients.
JOURNAL OF PATHOLOGY
(2023)
Article
Fisheries
G. M. Berge, T. Asgard, J. Kottmann, I. Lein
Summary: Atlantic halibut experience reduced or no feed intake during their spawning period. This study investigates the effect of a two-month starvation period on male Atlantic halibut, which simulates the naturally occurring spawning induced starvation. Results show that lipids are the preferred energy source during spawning-related starvation, and n-3 fatty acids are utilized more than other fatty acids. This information is important for formulating diets for re-feeding broodstock of annual spawners like the Atlantic halibut.
Article
Microbiology
Audrey C. Brown, Michelle D. Warthan, Anush Aryal, Shiwei Liu, Jennifer L. Guler
Summary: There is a dire need for effective treatments against microbial pathogens. Yet, the continuing emergence of drug resistance necessitates a deeper knowledge of how pathogens respond to treatments. We assessed the effect of nutrient limitation on the protozoan parasite that causes malaria and demonstrated that short-term growth under physiologically relevant mild nutrient stress triggers increased tolerance of a potent antimalarial drug. This finding highlights the important connections among nutrient levels, protective cellular pathways, and resistance evolution.
Article
Biochemistry & Molecular Biology
Haibin Wang, Dinah Loerke, Caroline Bruns, Rainer Mueller, Philipp-Alexander Koch, Dmytro Puchkov, Carsten Schultz, Volker Haucke
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Cell Biology
David Cruz-Garcia, Nathake Brouwers, Vivek Malhotra, Amy J. Curwin
JOURNAL OF CELL BIOLOGY
(2020)
Article
Biology
Ishier Raote, Andreas M. Ernst, Felix Campelo, James E. Rothman, Frederic Pincet, Vivek Malhotra
Correction
Cell Biology
Rianne Grond, Tineke Veenendaal, Juan M. Duran, Ishier Raote, Johan H. van Es, Sebastiaan Corstjens, Laura Delfgou, Benaissa El Haddouti, Vivek Malhotra, Catherine Rabouille
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Rianne Grond, Tineke Veenendaal, Juan M. Duran, Ishier Raote, Johan H. van Es, Sebastiaan Corstjens, Laura Delfgou, Benaissa El Haddouti, Vivek Malhotra, Catherine Rabouille
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Laasya Dhandapani, Marion C. Salzer, Juan M. Duran, Gabriele Zaffagnini, Cristian De Guirior, Maria Angeles Martinez-Zamora, Elvan Boke
Summary: Oocytes spend the majority of their lifetime in a primordial state, and understanding their cellular and molecular biology is crucial for studying cellular fitness and aging. This study developed methods for comparative characterization of Xenopus, mouse, and human primordial oocytes using live imaging. The results showed that all three species have active mitochondria, Golgi, and lysosomes in their primordial oocytes. Additionally, human and Xenopus oocytes have a Balbiani body characterized by dense accumulation of mitochondria, while mouse oocytes do not. This study provides insights into the organization of the cytoplasm in mammalian primordial oocytes and clarifies the advantages and limitations of different model organisms for studying oocyte dormancy.
JOURNAL OF CELL SCIENCE
(2022)
Review
Infectious Diseases
Candida Driemeyer, Diego R. Falci, Rita O. Oladele, Felix Bongomin, Bright K. Ocansey, Nelesh P. Govender, Martin Hoenigl, Jean Pierre Gangneux, Cornelia Lass-Florl, Oliver A. Cornely, Alexandre Alanio, Jesus Guinea, C. Orla Morrissey, Riina Rautemaa-Richardson, Arunaloke Chakrabarti, Jacques F. Meis, Caroline Bruns, Jannik Stemler, Alessandro C. Pasqualotto
Summary: Africa is a favorable environment for fungal infections due to high risk factors, but there are significant challenges in terms of laboratory infrastructure and availability of antifungal drugs in many African countries.
Article
Health Care Sciences & Services
Sibylle C. Mellinghoff, Caroline Bruns, Rouvier Al-Monajjed, Florian B. Cornely, Maria Grosheva, Jurgen A. Hampl, Carolin Jakob, Felix C. Koehler, Max Lechmann, Bijan Maged, Christina Otto-Lambertz, Robert Rongisch, Jule Rutz, Jon Salmanton-Garcia, Georg Schlachtenberger, Jannik Stemler, Janne Vehreschild, Sophia Wuelfing, Oliver A. Cornely, Blasius J. Liss
Summary: As routine data becomes essential in future healthcare research, harmonizing procedure codes is important for international research. Investigating surgical site infections can reveal health issues and facilitate global studies. A broader analysis of surgical procedures is necessary to understand the epidemiology of SSI and improve comparability of international trial findings.
BMC MEDICAL RESEARCH METHODOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Julio Olivera-Muzante, Sergio Fierro, Juan Manuel Duran, Jose Antognazza, Santiago Sanchez, Fernando Dutra, Fernando Baldi, Georgget Banchero
Summary: A short-term energy-protein supplementation during the peripartum in ewes has a positive effect on colostrum volume, viscosity, and composition, as well as the blood glucose of lambs at birth. It also reduces the occurrence of dystocia/stillbirth/birth injury as a cause of lamb mortality. However, it does not affect the length of gestation, duration or type of parturition, birth weight, lamb vigour, or lamb mortality to 72 h.
SMALL RUMINANT RESEARCH
(2022)
Article
Oncology
Merce Juliachs, Mireia Pujals, Chiara Bellio, Nathalie Meo-Evoli, Juan M. Duran, Esther Zamora, Mireia Pares, Anna Sunol, Olga Mendez, Alex Sanchez-Pla, Francesc Canals, Cristina Saura, Josep Villanueva
Summary: This study aims to find circulating biomarkers for monitoring the response to cancer therapy. The research found that the release of SOD2 protein during chemotherapy treatment is associated with tumor cell death, and the increase in its circulating levels correlates with treatment response, which can be used for non-invasive monitoring of breast cancer patients.
Article
Multidisciplinary Sciences
Gerard Cantero-Recasens, Carla Burballa, Yuki Ohkawa, Tomohiko Fukuda, Yoichiro Harada, Amy J. Curwin, Nathalie Brouwers, Gian A. Thun, Jianguo Gu, Ivo Gut, Naoyuki Taniguchi, Vivek Malhotra
Summary: The study reveals that FUT8 and specific mucin levels are increased in the distal inflamed colon of UC patients, and overexpression of FUT8 in colonic cells affects the secretion of MUC1, MUC2, and MUC5AC, thus modifying the biophysical properties of mucus.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jose Wojnacki, Agustin Leonardo Lujan, Nathalie Brouwers, Carla Aranda-Vallejo, Gonzalo Bigliani, Maria Pena Rodriguez, Ombretta Foresti, Vivek Malhotra
Summary: This study reveals that tetraspanin-8 sequesters syntaxin-2 to regulate the release of mucin and insulin, providing insights into the mechanisms of granule secretion.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Agustin Leonardo Lujan, Ombretta Foresti, Conor Sugden, Nathalie Brouwers, Alex Mateo Farre, Alessio Vignoli, Mahshid Azamian, Alicia Turner, Jose Wojnacki, Vivek Malhotra, Suzanne R. Pfeffer
Summary: TANGO2 primarily localizes to mitochondria and partially to mitochondria sites adjacent to lipid droplets and the endoplasmic reticulum. Cells lacking TANGO2 exhibit enlarged lipid droplets, with an increase in lysophosphatidic acid (LPA) and a decrease in its biosynthetic precursor phosphatidic acid (PA) observed through quantitative lipidomics. Our findings suggest that TANGO2 is linked to acyl-CoA metabolism, affecting the acylation of LPA to generate PA, and subsequently impacting the metabolism of fatty acids, generating reactive oxygen species and promoting lipid peroxidation. The enlarged lipid droplets result from the accumulation of peroxidized lipids and a defect in their catabolism. These insights help understand the physiological consequences of TANGO2 mutations and their association with metabolic crises and fatality under starvation and stress conditions.
Article
Health Care Sciences & Services
Sibylle Mellinghoff, Gero von Gersdorff, Caroline Bruns, Kerstin Albus, Vassiliki Dimitriou, Angela Steinbach, Mathias Schaller, Jorg Janne Vehreschild, Oliver A. Cornely, Blasius Janusch Liss
Summary: This study aims to compare the pneumonia rates between recently vaccinated patients and patients vaccinated more than 2 years ago, and to determine the dynamics of antipneumococcal antibody titers in hemodialysis patients. The results will increase physician adherence to current recommendations and provide evidence for future guidelines.
JMIR RESEARCH PROTOCOLS
(2023)
Article
Microbiology
Jannik Stemler, Caroline Bruns, Sibylle C. Mellinghoff, Nael Alakel, Hamdi Akan, Michelle Ananda-Rajah, Jutta Auberger, Peter Bojko, Pranatharthi H. Chandrasekar, Methee Chayakulkeeree, Jose A. Cozzi, Elizabeth A. de Kort, Andreas H. Groll, Christopher H. Heath, Larissa Henze, Marcos Hernandez Jimenez, Souha S. Kanj, Nina Khanna, Michael Koldehoff, Dong-Gun Lee, Alina Mager, Francesco Marchesi, Rodrigo Martino-Bufarull, Marcio Nucci, Jarmo Oksi, Livio Pagano, Bob Phillips, Juergen Prattes, Athina Pyrpasopoulou, Werner Rabitsch, Enrico Schalk, Martin Schmidt-Hieber, Neeraj Sidharthan, Pere Soler-Palacin, Anat Stern, Barbora Weinbergerova, Aline El Zakhem, Oliver A. Cornely, Philipp Koehler