期刊
JOURNAL OF CELL BIOLOGY
卷 200, 期 3, 页码 249-258出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201206010
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资金
- Uehara Memorial Foundation
- Institut National de la Sante et de la Recherche Medicale
- Agence Nationale pour la Recherche
- Institut National du Cancer
- Fondation Association pour la Recherche contre le Cancer
- La Ligue contre le Cancer
Intermediate filaments (IFs) are components of the cytoskeleton involved in most cellular functions, including cell migration. Primary astrocytes mainly express glial fibrillary acidic protein, vimentin, and nestin, which are essential for migration. In a wound-induced migration assay, IFs reorganized to form a polarized network that was coextensive with microtubules in cell protrusions. We found that the tumor suppressor adenomatous polyposis coli (APC) was required for microtubule interaction with IFs and for microtubule-dependent rearrangements of IFs during astrocyte migration. We also show that loss or truncation of APC correlated with the disorganization of the IF network in glioma and carcinoma cells. In migrating astrocytes, vimentin-associated APC colocalized with microtubules. APC directly bound polymerized vimentin via its armadillo repeats. This binding domain promoted vimentin polymerization in vitro and contributed to the elongation of IFs along microtubules. These results point to APC as a crucial regulator of IF organization and confirm its fundamental role in the coordinated regulation of cytoskeletons.
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