Editorial Material
Cell Biology
Matthew J. Eramo, Rajendra Gurung, Christina A. Mitchell, Meagan J. McGrath
Summary: Autophagic lysosome reformation (ALR) is crucial for recycling autophagy membranes to form lysosomes. INPP5K, an enzyme that converts PtdIns(4,5)P-2 to PtdIns4P, plays a key role in this process in skeletal muscle. Mutations in INPP5K can lead to muscular dystrophy by disrupting ALR and lysosome homeostasis in muscles.
Article
Plant Sciences
Lizhen Song, Yanning Wang, Zhiai Guo, Sin M. Lam, Guanghou Shui, Youfa Cheng
Summary: Phosphoinositides play vital roles in plant growth and development, with SAC6 to SAC8 identified as crucial phosphatases in Arabidopsis involved in auxin signaling and embryo development. These genes exhibit specific and overlapping expression patterns in seedlings and embryos, and mutations can lead to abnormal seed and cotyledon development. Mutants of SAC6 to SAC8 show delayed or lethal embryo development, with disruptions in auxin maxima and increased levels of PtdIns4P and PtdIns(4,5)P2. Dysfunction in these genes also affects protein trafficking from trans-Golgi network/early endosome to plasma membrane, impacting auxin-mediated development in Arabidopsis.
Article
Cell Biology
Muhmmad Omar-Hmeadi, Alenka Gucek, Sebastian Barg
Summary: Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) is a crucial phospholipid in regulated exocytosis and some forms of endocytosis. The accumulation of PI(4,5)P2 at the release site inhibits fusion pore expansion and cargo release through an unconventional endocytic mechanism. This process involves the recruitment of endocytic fission machinery and is independent of clathrin-mediated endocytosis.
Article
Multidisciplinary Sciences
Nathan R. Zaccai, Zuzana Kadlecova, Veronica Kane Dickson, Kseniya Korobchevskaya, Jan Kamenicky, Oleksiy Kovtun, Perunthottathu K. Umasankar, Antoni G. Wrobel, Jonathan G. G. Kaufman, Sally R. Gray, Kun Qu, Philip R. Evans, Marco Fritzsche, Filip Sroubek, Stefan Hoening, John A. G. Briggs, Bernard T. Kelly, David J. Owen, Linton M. Traub
Summary: Clathrin-mediated endocytosis (CME) is a key mechanism for controlling the cell surface proteome in mammalian cells. This study shows that FCHO plays a crucial role in this process by marking the initiation sites of clathrin-coated pits (CCPs) and interacting with other proteins to drive the growth and maturation of CCPs.
Article
Biochemistry & Molecular Biology
Maximilian M. Gass, Sarah Borkowsky, Marie-Luise Lotz, Rebecca Siwek, Rita Schroeter, Pavel Nedvetsky, Stefan Luschnig, Astrid Rohlmann, Markus Missler, Michael P. Krahn
Summary: Drosophila nephrocytes, similar to mammalian podocytes, exhibit characteristics of epithelial cells. Research has shown that phospholipid PI(4,5)P2 plays a crucial role in slit diaphragm formation, while PI(3,4,5)P3 has minimal impact on nephrocyte function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Federico Gulluni, Lorenzo Prever, Huayi Li, Petra Krafcikova, Ilaria Corrado, Wen-Ting Lo, Jean Piero Margaria, Anlu Chen, Maria Chiara De Santis, Sophie J. Cnudde, Joseph Fogerty, Alex Yuan, Alberto Massarotti, Nasrin Torabi Sarijalo, Oscar Vadas, Roger L. Williams, Marcus Thelen, David R. Powell, Markus Schueler, Michael S. Wiesener, Tamas Balla, Hagit N. Baris, Dov Tiosano, Brian M. McDermott, Brian D. Perkins, Alessandra Ghigo, Miriam Martini, Volker Haucke, Evzen Boura, Giorgio Roberto Merlo, David A. Buchner, Emilio Hirsch
Summary: The cytokinetic process in the lens, which depends on PI3K-C2alpha, PI(3,4)P-2, and VPS36, is essential for preventing premature senescence and cataract. Loss of these components impairs cytokinesis and leads to cell damage, triggering senescence in the lens of fish, mice, and humans.
Article
Multidisciplinary Sciences
Darshini Jeyasimman, Bilge Ercan, Dennis Dharmawan, Tomoki Naito, Jingbo Sun, Yasunori Saheki
Summary: Cellular membranes have unique lipid compositions, and cells maintain endosomal lipid homeostasis through lipid transfer proteins and phosphatases to prevent the accumulation of PI(4,5)P-2 on endosomes.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Manjari Mishra, Shobhna Kapoor
Summary: Virulence-associated glycolipids from Mycobacterium tuberculosis play a crucial role in infection, altering the host cell membrane properties and functions. The interaction between Mtb lipids and host membranes affects membrane abundance/organization of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P-2) and impacts plasma membrane-cytoskeletal adhesion forces. These findings enhance our understanding of how Mtb lipids manipulate host cell processes during infection.
FARADAY DISCUSSIONS
(2021)
Article
Cell Biology
Alex G. Batrouni, Nirmalya Bag, Henry T. Phan, Barbara A. Baird, Jeremy M. Baskin
Summary: Phosphatidylinositol 4-kinase III alpha (PI4KIII alpha) is responsible for generating phosphatidylinositol (4)-phosphate at the plasma membrane, and the differential palmitoylation patterns of its anchor protein EFR3B control its distribution and phosphoinositide homeostasis.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yuta Yamazaki, Keiko Kono
Summary: This study reveals that clathrin-mediated trafficking of phospholipid flippases is critical for the recruitment of the exocyst complex to the damage site during plasma membrane/cell wall repair in budding yeast.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Chan Liu, Zhimin Li, Dan Tian, Mei Xu, Jianwei Pan, Haijun Wu, Chao Wang, Marisa S. Otegui
Summary: The Arabidopsis AP1/2 beta adaptins play a critical role in plants, regulating the formation of the pollen wall and reproduction by mediating the trafficking of ABC transporters during pollen development.
Article
Oncology
Rin Liu, Satoru Shinriki, Manabu Maeshiro, Mayumi Hirayama, Hirofumi Jono, Ryoji Yoshida, Hideki Nakayama, Hirotaka Matsui
Summary: This study found that the loss of CYLD restricted the clustering of EGFR in lipid rafts and inhibited CTX-induced apoptosis. Disruption of lipid rafts restored the endocytic function of EGFR and the activity of CTX in CYLD-downregulated cells.
Review
Cell Biology
Sana A. A. Fadil, Chris Janetopoulos
Summary: This article explores the mechanisms regulating cell migration during fruiting body formation in Dictyostelium discoideum, finding that cAMP is released through expulsion during contractile vacuole ejection. The vacuole localizes to the rear of the cell and is regulated by membrane lipids, including PI(4,5)P2. The exocyst complex, mediated in part by PI(4,5)P2-binding, is essential for exocytosis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jing Xu, Xiaolu Zhao, Fengbiao Mao, Venkatesha Basrur, Beatrix Ueberheide, Brian T. Chait, C. David Allis, Sean D. Taverna, Shan Gao, Wei Wang, Yifan Liu
Summary: In this study, the EZL1 complex in the protist Tetrahymena thermophila was characterized, revealing its essential role in histone methylation, heterochromatin formation, and genome rearrangement. The interaction between the EZL1 complex and EMA1, as well as PDD1, reinforces its chromatin association and contributes to the dynamic distribution of Polycomb bodies in Tetrahymena development. These findings provide a molecular mechanism linking RNAi and Polycomb repression, ultimately regulating nuclear bodies and genome organization.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Carol H. Pong, Jade E. Peace, Christopher J. Harmer, Ruth M. Hall
Summary: In Gram-negative bacteria, the contribution of IS26 to the spread of antibiotic resistance and other genes is significant due to its unique mechanistic features, such as causing deletion of adjacent DNA segments and using two distinct reaction modes for cointegrate formation. The high frequency of the targeted conservative reaction mode is also crucial for its spread. However, the detailed mechanism of this reaction and the role of the RuvABC system in HJ resolution still need further investigation.
MICROBIOLOGY SPECTRUM
(2023)
