期刊
JOURNAL OF CELL BIOLOGY
卷 194, 期 3, 页码 367-375出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201012132
关键词
-
类别
资金
- Cancer Research UK [C1521/A8182]
- Biotechnology and Biological Sciences Research Council [BB/H009957/1]
- Oxford University
- Biotechnology and Biological Sciences Research Council [BB/H009957/1] Funding Source: researchfish
- Cancer Research UK [12353] Funding Source: researchfish
- BBSRC [BB/H009957/1] Funding Source: UKRI
Poly adenosine diphosphate (ADP)-ribosylation (PARylation) by poly ADP-ribose (PAR) polymerases (PARPs) is an early response to DNA double-strand breaks (DSBs). In this paper, we exploit Dictyostelium discoideum to uncover a novel role for PARylation in regulating nonhomologous end joining (NHEJ). PARylation occurred at single-strand breaks, and two PARPs, Adprt1b and Adprt2, were required for resistance to this kind of DNA damage. In contrast, although Adprt1b was dispensable for PARylation at DSBs, Adprt1a and, to a lesser extent, Adprt2 were required for this event. Disruption of adprt2 had a subtle impact on the ability of cells to perform NHEJ. However, disruption of adprt1a decreased the ability of cells to perform end joining with a concomitant increase in homologous recombination. PAR-dependent regulation of NHEJ was achieved through promoting recruitment and/or retention of Ku at DSBs. Furthermore, a PAR interaction motif in Ku70 was required for this regulation and efficient NHEJ. These data illustrate that PARylation at DSBs promotes NHEJ through recruitment or retention of repair factors at sites of DNA damage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据