Article
Plant Sciences
Jesus Montiel, Dugald Reid, Thomas H. Gronbaek, Caroline M. Benfeldt, Euan K. James, Thomas Ott, Franck A. Ditengou, Marcin Nadzieja, Simon Kelly, Jens Stougaard
Summary: This study reports intercellular infection of the model legume Lotus japonicus by the IRBG74 strain and reveals the genetic requirements and differences in the pathways governing this process compared to intracellular infection. The results indicate a differential requirement for cytokinin signaling during intercellular rhizobial entry, highlighting distinct modalities of inter- and intracellular infection mechanisms in L. japonicus.
Article
Oncology
Janine M. DeBlasi, Aimee Falzone, Samantha Caldwell, Nicolas Prieto-Farigua, Justin R. Prigge, Edward E. Schmidt, Iok In Christine Chio, Florian A. Karreth, Gina M. DeNicola
Summary: Mutations in the KEAP1-NRF2 pathway are common in NSCLC and contribute to therapy resistance and poor outcomes. The impact of KEAP1 and NRF2 mutations on lung tumor initiation and progression was comprehensively investigated using mouse models. The study revealed the context-dependence and activity threshold for NRF2 in the lung tumorigenic process.
Article
Biochemistry & Molecular Biology
Jiajun Ding, Qian Gou, Xiao Jia, Qian Liu, Jianhua Jin, Juanjuan Shi, Yongzhong Hou
Summary: The study found that AMPK-induced PPARδ-S50 phosphorylation, accumulation of misfolded PPARδ protein, and inhibition of PPARδ transcription activity can suppress colon tumor formation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biology
Soren Helweg Dam, Lars Ronn Olsen, Kristoffer Vitting-Seerup
Summary: This study used pairedGSEA to analyze a large number of RNA-seq datasets and found that alternative splicing contributes to 48.1% of the biological signal in expression analyses on average. Both expression and splicing convey shared and distinct biological signals. These findings establish alternative splicing as a major regulator of the human condition and suggest that most contemporary RNA-seq studies likely miss out on critical biological insights.
Article
Multidisciplinary Sciences
Viviane Nascimento Da Conceicao, Yuyang Sun, Karthik Ramachandran, Arun Chauhan, Amritha Raveendran, Manigandan Venkatesan, Bony DeKumar, Soumya Maity, Neelanjan Vishnu, George A. Kotsakis, Paul F. Worley, Donald L. Gill, Bibhuti B. Mishra, Muniswamy Madesh, Brij B. Singh
Summary: The transformation of naive macrophages into M1 or M2 phenotypes is regulated by distinct Ca2+ entry channels. Blocking Ca2+ entry can suppress signaling events related to cytokine production and reduce the inflammatory response of macrophages, depending on the expression levels of Orai1 and TRPC1 channels.
Article
Cell Biology
Ingo Amm, Marion Weberruss, Andrea Hellwig, Johannes Schwarz, Marianna Tatarek-Nossol, Christian Luechtenborg, Martina Kallas, Britta Bruegger, Ed Hurt, Wolfram Antonin
Summary: Amm et al. discovered a genetic interaction between the amphipathic helix in Ndc1 and similar motifs in Nup53 and Nup59, indicating that the formation of nuclear pores relies on a balance of amphipathic motifs in different nucleoporins. They also found that Ndc1's transmembrane domain interacts with Nup120 and Nup133, members of the pore membrane coating Y-complex. Furthermore, they identified an amphipathic helix in Ndc1's C-terminal domain that binds to highly curved liposomes and affects intracellular membrane organization in yeast.
JOURNAL OF CELL BIOLOGY
(2023)
Editorial Material
Neurosciences
Ikenna P. Njoku, Kenneth Y. Kwan
Summary: In this study, the authors use novel molecular tools to manipulate microtubule nucleation and dynamics, and reveal the distinct microtubule networks involved in neuronal migration and axon extension.
Article
Multidisciplinary Sciences
Jisca Majolee, Fabienne Podieh, Peter L. Hordijk, Igor Kovacevic
Summary: Research shows a strong, positive correlation between Rac1 activity and its level of ubiquitination, but GDI dissociation does not predispose Rac1 to ubiquitination.
Article
Biochemistry & Molecular Biology
Hannes Schihada, Thomas M. Klompstra, Laura J. Humphrys, Igor Cervenka, Shamim Dadvar, Peter Kolb, Jorge L. Ruas, Gunnar Schulte
Summary: The G protein-coupled receptor GPR35 is involved in oncogenic signaling, bacterial infections, and inflammatory bowel disease within the intestine. This study reveals that GPR35 has two isoforms with distinct promoters and alternative splicing. The longer isoform limits G protein activation but enhances receptor-beta-arrestin interaction. Structural analysis suggests that a disulfide bridge between the N-terminus and extracellular loop 3 is crucial for G protein activation, while an additional cysteine from the extended N-terminus of the longer isoform limits agonist-induced receptor-beta-arrestin2 interaction. The findings provide insights for developing isoform-specific GPR35 ligands for mechanism-based therapies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Mark S. Lee, Peter J. Tuohy, Caleb Y. Kim, Katrina Lichauco, Heather L. Parrish, Koenraad Van Doorslaer, Michael S. Kuhns, Richard N. McLaughlin
Summary: Through evolution, purifying selection has shaped motifs in the CD4 protein that enhance its response to MHCII molecules. The importance of CD4-Lck interactions is derived from the balancing activity of an inhibitory motif, as well as motifs that direct CD4-Lck pairs to specific membrane compartments.
Article
Multidisciplinary Sciences
Xin Xu, Zhe Wei, Guangyu Wu
Summary: This study reveals the importance of multiple motifs in the trafficking of G protein-coupled receptors (GPCRs), with a specific leucine residue being involved in synaptic targeting and a dileucine (LL) motif being required for dendritic and post-synaptic traffic of all GPCRs studied.
Review
Cell Biology
Huiyin Lan, Yi Sun
Summary: Proper DNA damage response and repair mechanisms are crucial for cellular homeostasis and genomic integrity, with abnormalities often observed in human cancers. FBXW7, as a tumor suppressor, also plays critical roles in DDR and repair processes, offering potential implications for translational research and anticancer therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kei Segawa, Kazuhiko Igarashi, Kazutaka Murayama
Summary: The function of the transcription factor BACH1 is regulated by heme binding to multiple Cys-Pro (CP) motifs within its intrinsically disordered regions. The individual CP motifs contribute to the regulation of BACH1 activity by accepting heme in different coordination manners and their spatial locations are important for their individual functions. The presence of multiple CP motifs with distinct roles ensures the multifaceted, strict regulation of BACH1 by heme.
Article
Multidisciplinary Sciences
A. F. Garcia, E. A. Crummy, I. G. Webb, M. N. Nooney, S. M. Ferguson
Summary: Processing within the anterior cingulate cortex is crucial for appropriate behavior, and dysfunction following chronic drug use is thought to play a major role in drug addiction. Two types of cortical pyramidal projection neurons regulate distinct features of a drug experience, its positive and negative aspects, respectively, supporting a revised theory of cortical function in addiction.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Zhiyuan Chen, Mohamed Nadhir Djekidel, Yi Zhang
Summary: In mouse preimplantation embryos, H2AK119ub1 and H3K27me3 have distinct genome-wide dynamics. Loss of H2AK119ub1 results in premature activation of developmental genes, while loss of H3K27me3 does not.
Article
Chemistry, Multidisciplinary
Oleksandr V. Kuznietsov, George D. Tsibidis, Anatoliy V. Demchishin, Anatoliy A. Demchishin, Volodymyr Babizhetskyy, Ivan Saldan, Stefano Bellucci, Iaroslav Gnilitskyi
Summary: Research on heating Ti-Fe multilayer structures with femtosecond laser pulses to promote the formation of intermetallic compounds and surface nanostructuring. Experimental investigation on the effects of Laser Induced Periodic Surface Structures (LIPSS) on Ti-Fe multilayer thin films.
Article
Chemistry, Physical
L. Museur, A. Manousaki, D. Anglos, G. D. Tsibidis, A. Kanaev
Summary: The study investigates the reaction control by laser light on TiO2 monocrystals, showing different surface morphologies and the crucial role of the critical temporal delay in the formation of grooves and periodic structures.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Physical
J. J. J. Nivas, E. Allahyari, E. Skoulas, R. Bruzzese, R. Fittipaldi, G. D. Tsibidis, E. Stratakis, S. Amoruso
Summary: The experimental investigation focused on laser induced periodic surface structures on crystalline silicon at different angles of incidence and for different laser wavelengths. The period of the ripples displayed the expected angular dependence, while the grooves period exhibited a decreasing angular dependence. Simulations highlighted the influence of hydrothermal waves on the formation of grooves.
APPLIED SURFACE SCIENCE
(2021)
Article
Optics
Suzana Petrovic, G. D. Tsibidis, A. Kovacevic, N. Bozinovic, D. Perusko, A. Mimidis, A. Manousaki, E. Stratakis
Summary: The experimental study investigated the static and dynamic femtosecond laser ablation of the multilayer 15x(Ti/Zr)/Si system, analyzing surface morphology and elemental composition to study the layer-by-layer selective laser ablation mechanism. The dynamic multipulse irradiation demonstrated the formation of lines with laser-induced periodic surface structures (LIPSS) at different laser parameters, showing a dependence on the effective number of pulses and laser polarization as well as material properties. Simulations were conducted to explore the thermal response of the multiple layered structure 15x(Ti/Zr) after static single pulse irradiation for better interpretation of the experimental results.
EUROPEAN PHYSICAL JOURNAL D
(2021)
Article
Biochemistry & Molecular Biology
Stella Maragkaki, Panagiotis C. Lingos, George D. Tsibidis, George Deligeorgis, Emmanuel Stratakis
Summary: The study explores the impact of laser polarization on pre-patterned surfaces and the formation of periodic structures in different directions, revealing that laser-induced periodic surface structures exhibit different periodic characteristics depending on the polarization direction.
Article
Chemistry, Multidisciplinary
Matina Vlahou, Fotis Fraggelakis, Phanee Manganas, George D. Tsibidis, Anthi Ranella, Emmanuel Stratakis
Summary: This study investigates the changes in the topography of stainless steel induced by femtosecond laser with double pulses. The effects of parameters such as fluence, energy dose, and interpulse delay on the produced patterns are revealed. The study demonstrates that different spatial characteristics of surface patterns exhibit different wetting and cell adhesion properties, suggesting the potential application of this methodology in the development of tailored surfaces for functional implants.
Article
Optics
George D. Tsibidis, Panagiotis Lingos, Emmanuel Stratakis
Summary: Femtosecond pulsed lasers have been widely used for precise materials structuring on micro- and nano-scales. Understanding the fundamental laser-matter interaction processes is crucial for efficient material processing and the formation of laser-induced periodic surface structures (LIPSS). This study predicts the pulse-by-pulse formation of LIPSS on metals and highlights the importance of electron diffusion and electron-phonon coupling in the formation of stable periodic structures.
Article
Metallurgy & Metallurgical Engineering
G. D. Tsibidis, E. Stratakis
Summary: This study emphasizes the influence of the substrate material on the thermal characteristics of irradiated films, particularly with a greater impact at smaller film sizes. Silicon and fused silica were chosen as substrate materials, and the thermal response and damage thresholds of irradiated solids were evaluated. The significant influence of the substrate can provide reference for the design of laser-based fabrication setups and processing schemes.
JOURNAL OF CENTRAL SOUTH UNIVERSITY
(2022)
Article
Physics, Applied
George D. Tsibidis, Emmanuel Stratakis
Summary: The employment of ultrashort laser sources at the mid-infrared spectral region for dielectrics is expected to open innovative routes for laser patterning and a wealth of exciting applications in optics and photonics. A consistent analysis of the interaction between long wavelength femtosecond pulses and dielectric materials is presented to elucidate the material response to irradiation with mid-IR laser sources. Simulation results and correlations of electron densities with surface plasmons (SPs) and the threshold of SP excitation are discussed, providing guidance for the development of an innovative approach to surface patterning using strong mid-IR pulses.
APPLIED PHYSICS LETTERS
(2023)
Article
Optics
G. D. Tsibidis, D. Mansour, E. Stratakis
Summary: This study investigates the impact of various parameters on the damage threshold of different metals and proposes a multiscale physical model that accurately predicts the damage and surface modification processes in laser processing.
OPTICS AND LASER TECHNOLOGY
(2022)
Correction
Physics, Applied
George D. Tsibidis, Emmanuel Stratakis
APPLIED PHYSICS LETTERS
(2023)
Article
Optics
Panagiotis Lingos, George Perrakis, Odysseas Tsilipakos, George D. Tsibidis, Emmanuel Stratakis
Summary: This study reveals the mechanisms behind the formation of nanoscale laser-induced periodic surface structures on thin metal films of the size of the optical penetration depth. This unexplored area is expected to open new routes for laser patterning and has the potential for exciting applications in optics, photonics, and sensing.
OPTICS AND LASER TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Chrysanthi Voutyraki, Alexandros Choromidis, Anastasia Meligkounaki, Nikolaos Andreas Vlachopoulos, Vasiliki Theodorou, Sofia Grammenoudi, Emmanouil Athanasiadis, Sara Monticelli, Angela Giangrande, Christos Delidakis, Evanthia Zacharioudaki
Summary: This study provides new insights into the interaction between a neural stem cell tumor and its microenvironment in Drosophila. The tumor growth is influenced by intrinsic factors in the tumor cells and the presence of phagocytic blood cells in the host, which can both promote and restrict tumor growth respectively.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Optics
Fotis Fraggelakis, George D. Tsibidis, Emmanuel Stratakis
Summary: This study presents a novel approach to tailor laser-induced surface topography using double femtosecond laser pulses. The researchers found that different types of pulses and the delay between pulses have a significant impact on the surface profile, and the formation of complex surface morphologies can be controlled by modulating the spatial intensity and number of pulses. In addition, a multiscale model combining electron excitation with melt hydrodynamics was proposed to explain the generation of complex patterns.
OPTO-ELECTRONIC ADVANCES
(2022)
Proceedings Paper
Engineering, Electrical & Electronic
F. Fraggelakis, G. D. Tsibidis, E. Stratakis
2021 CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE & EUROPEAN QUANTUM ELECTRONICS CONFERENCE (CLEO/EUROPE-EQEC)
(2021)