期刊
NEURODEGENERATIVE DISEASES
卷 15, 期 1, 页码 50-57出版社
KARGER
DOI: 10.1159/000369465
关键词
Alzheimer's disease; Pathological ageing; Amyloid precursor protein; Amyloid-beta; Brain; lmnnunoprecipitation; Mass spectrometry
资金
- Leonard Wolfson Experimental Neurology Centre
- Swedish Research Council
- Knut and Alice Wallenberg Foundation
- JPND BIOMARKAPD project
- EMIF-AD
- Goteborg Medical Society
- Swedish Brain Power
- Stiftelsen Gamla Tjanarinnor
- Alzheimerfonden
- Tammaryn Lashley
- Alzheimer's Research UK fellowship
- National Institute for Health Research Biomedical Research Unit in Dementia based at University College London Hospitals, University College London
- Alzheimers Research UK [ARUK-Network2011-6-ICE] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0508-10123, NF-SI-0513-10134] Funding Source: researchfish
Background: Senile plaques in Alzheimer's disease (AD) are composed of amyloid-beta (A beta) especially N-truncated forms including A beta(4-42). These are thought to be neurotoxic. However, individuals may live for decades with biomarker evidence of cerebral beta-amyloidosis (positive amyloid PET imaging and/or low cerebrospinal fluid levels of the 42 amino acid form of A beta) without cognitive impairment. This condition may be termed pathological ageing (PA). Objective: To investigate whether there is a difference in the cerebral A beta fragment pattern in brain specimens from non-demented (PA) and demented (AD) individuals expressing the full neuropathological triad of AD (senile plaques, neurofibrillary tangles and neurodegeneration). Methods: We extracted A beta using formic acid and hybrid (6E10 and 4G8) immunoprecipitation from fresh-frozen temporal cortex tissue of 6 elderly individuals (mean age +/- SD:89 +/- 3.5 years) with PA and 10 patients with AD (mean age +/- SD: 72 +/- 8.5 years). The full spectrum of A beta peptides was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Results: AD patients had generally more N-terminally truncated and pyroglutamate-modified A beta than PA patients, whereas PA patients had on average more A beta(1-40) than AD patients. Conclusion: Senile plaques in AD may have an AB fragment composition distinct from PA with more N-terminally and pyroglutamate-modified A beta peptides that may be linked to neurotoxicity. (C) 2015 S. Karger AG, Basel
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