期刊
NEUROCHEMISTRY INTERNATIONAL
卷 91, 期 -, 页码 1-12出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuint.2015.10.004
关键词
Huntington's disease; Luteolin; Oxidative stress; Nuclear factor (erythroid-derived-2)-like 2; Superoxide dismutase; Glutamate-cysteine ligase catalytic subunit
资金
- Fundacao para a Ciencia e a Tecnologia (FCT), Portugal [PTDC/SAU-FCF/108056/2008, PEst-C/SAU/LA0001/2013-2014, PEst-C/QUI/UI0062/2013, PEst-OE/AGR/UI0681/2011]
- COMPETE- Programa Operacional Factores de Competitividade
- European Union (FEDER- Fundo Europeu de Desenvolvimento Regional)
- FCT [SFRH/BD/41285/2007]
- POPH- Programa Operacional Potencial Humano
- European Union
- QREN
- Fundação para a Ciência e a Tecnologia [SFRH/BD/41285/2007, PEst-OE/AGR/UI0681/2011] Funding Source: FCT
Huntington's disease (HD) is a polyglutamine-expansion neurodegenerative disorder caused by increased number of CAG repeats in the HIT gene, encoding for the huntingtin protein. The mutation is linked to several intracellular mechanisms, including oxidative stress. Flavones are compounds with a protective role in neurodegenerative pathologies. In the present study we analyzed the protective effect of luteolin (Lut, 3',4',5,7-tetrahydroxyflavone) and four luteolin derivatives bearing 3-alkyl chains of 1, 4, 6 and 10 carbons (Lut-C1, Lut-C4, Lut-C6, Lut-C10) in striatal cells derived from HD knock-in mice expressing mutant Htt (STHdh(Q111/Q111)) versus wild-type striatal cells (STHdh(Q7/Q7)). HD cells showed increased caspase-3-like activity and intracellular reactive oxygen species (ROS), which were significantly decreased following treatment with Lut-C4 and Lut-C6 under concentrations that enhanced cell viability. Interestingly, Lut-C4 and Lut-C6 rose the nuclear levels of phospho(Ser40)-nuclear factor (erythroid-derived-2)-like 2 (Nrf2) and Nr12/ARE transcriptional activity. Concordantly with increased Nrf2/ARE transcription, Lut-C6 enhanced superoxide dismutase I (SOD1) mRNA and SOD activity and glutamate-cysteine ligase catalytic subunit (GCLc) mRNA and protein levels, while Lut-C4 induced mRNA levels of GCLc only in mutant striatal cells. Data suggest that Lut-C6 luteolin derivative (in particular) might be relevant for the development of antioxidant strategies in HD. (C) 2015 Elsevier Ltd. All rights reserved.
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