Article
Chemistry, Multidisciplinary
Larissa Socrier, Somayeh Ahadi, Mathias Bosse, Cindy Montag, Daniel B. B. Werz, Claudia Steinem
Summary: This study presents a method to investigate membrane structure using photo-sensitive lipids and explore the effect of photo-isomerization on liquid-ordered domains. By synthesizing glycosphingolipids with different positions of azobenzene moiety, the reorganization of membrane structure under light stimulation was examined, and it was found that trans-cis photo-isomerization leads to the formation of liquid-disordered domains within liquid-ordered domains. The study also highlights the impact of domain reorganization on protein-lipid interactions.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Szymczak-Kulus, Sascha Weidler, Anna Bereznicka, Krzysztof Mikolajczyk, Radoslaw Kaczmarek, Bartosz Bednarz, Tao Zhang, Anna Urbaniak, Mariusz Olczak, Enoch Y. Park, Edyta Majorczyk, Katarzyna Kapczynska, Jolanta Lukasiewicz, Manfred Wuhrer, Carlo Unverzagt, Marcin Czerwinski
Summary: Human Gb3/CD77 synthase is a promiscuous glycosyltransferase that can produce various glycosphingolipids recognized by Shiga toxins. Experimental evidence shows that this enzyme can synthesize P1 glycotope on N-glycans and glycoproteins, allowing Shiga toxins to enter and kill cells. The results suggest a new mechanism for Shiga toxin pathology beyond canonical GSL receptors.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Organic
Intzar Ali, M. V. Kamala Lakshmi, Ramu Sridhar Perali
Summary: The Mislow-Evans rearrangement was utilized to construct digitoxose-derived glycals used in the synthesis of cardenolides digitoxin, digoxin, and gitoxin. The scalability of the trisaccharide synthesis was demonstrated by performing reactions on a multigram scale. Glycosylation reactions between the synthesized digoxin glycal donor and aglycons digoxigenin and gitoxigenin yielded novel cardenolide derivatives.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Anna Bereznicka, Krzysztof Mikolajczyk, Katarzyna Szymczak-Kulus, Katarzyna Kapczynska, Edyta Majorczyk, Anna Modlinska, Tomasz Piasecki, Radoslaw Kaczmarek, Marcin Czerwinski
Summary: Most glycosyltransferases have strong substrate specificity, with human Gb3/CD77 synthase mainly synthesizing the Gal alpha 1 -> 4Gal moiety on glycosphingolipids, while its pigeon homolog prefers glycoproteins. This study characterized two Gb3/CD77 synthase paralogs in pigeons and found that they have different specificities in acceptors, which may be related to the difference in expression profiles of Gal alpha 1 -> 4Gal-terminated glycoconjugates between neoavians and mammals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ekaterina D. Kazakova, Dmitry V. Yashunsky, Nikolay E. Nifantiev
Summary: The synthesis of the spacered A trisaccharide was achieved by employing a glycosyl donor with stereocontrolling groups, resulting in efficient and stereoselective formation of the required alpha-glycosylation product. The final products were deprotected and subjected to spacer biotinylation for use in biochemical studies.
Review
Biochemistry & Molecular Biology
Lina Siukstaite, Anne Imberty, Winfried Roemer
Summary: Understanding the interactions between lectins and glycolipids, particularly focusing on the binding of lectins to Gb3, is crucial for investigating cellular membrane dynamics and lectin functions. Different Gb3-binding lectins, such as Stx and LecA, induce distinct plasma membrane domains and utilize different transport pathways, demonstrating structural versatility and evolution of specificity and carbohydrate recognition in different kingdoms of life.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Chemistry, Medicinal
Zhicheng Liu, Xia Li, Zhongkai Lu, Xinfang Qin, Haofei Hong, Zhifang Zhou, Roland J. Pieters, Jie Shi, Zhimeng Wu
Summary: In this study, a pentameric antibody recruiting chimera was developed for targeted immunotherapy of colorectal cancer by specifically binding to the tumor-associated carbohydrate antigen Gb3. The chimera demonstrated potent cytotoxic effects and inhibited tumor growth in a mouse model.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Immunology
Silje N. Ramstad, Arne M. Taxt, Umaer Naseer, Yngvild Wasteson, Jorgen V. Bjornholt, Lin T. Brandal
Summary: The study found that some antibiotics can increase the production of Shiga toxin, while others do not. STEC O104:H4 was the serotype that exhibited the strongest response to antimicrobial exposure with an increase in stx2a transcription and Stx production.
MICROBIAL PATHOGENESIS
(2021)
Review
Immunology
Izabella de Macedo Henrique, Flavia Sacerdoti, Raissa Lozzardo Ferreira, Camila Henrique, Maria Marta Amaral, Roxane Maria Fontes Piazza, Daniela Luz
Summary: This article introduces the structure of Shiga toxins (Stx) and the bacteria that produce them, as well as different types of antibody molecules for Stx neutralization and their potential applications.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Wouter J. C. Feitz, Romy Bouwmeester, Thea J. A. M. van der Velden, Susan Goorden, Christoph Licht, Lambert P. J. W. van den Heuvel, Nicole C. A. J. van de Kar
Summary: This study suggests that inhibiting the synthesis of Gb3 could be a potential therapeutic target for protecting against (further) endothelial damage caused by Stx in the future.
Article
Pharmacology & Pharmacy
Zeinab Mohseni, Hamid Sedighian, Raheleh Halabian, Jafar Amani, Elham Behzadi, Abbas Ali Imani Fooladi
Summary: The study synthesized a novel antitumor candidate DT389-STXB fusion protein, which exhibited potential cytotoxicity against cancer cells, especially towards GB3-expressing breast cancer cells, demonstrating high affinity and dose-dependent cytotoxicity.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Plant Sciences
Richard Strasser, Georg Seifert, Monika S. Doblin, Kim L. Johnson, Colin Ruprecht, Fabian Pfrengle, Antony Bacic, Jose M. Estevez
Summary: Glycosylation is a crucial modification process that can alter the biological function of proteins and lipids, affecting the development and physiology of an organism. While challenges remain in understanding plant glycosylation, high throughput approaches may help to address these gaps, revolutionizing the identification and characterization of unknown plant glycosyltransferases and hydrolases.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Danyang Li, Somshuvra Mukhopadhyay
Summary: The CMP-sialic acid transporter SLC35A1 and UDP-galactose transporter SLC35A2 have distinctive substrate specificities determined by three key pockets in their central cavity: the nucleobase pocket, middle pocket, and sugar pocket. Critical residues such as Y214 and G239 discriminate between different substrates, while residues like Q129 and Q278 interact specifically with certain substrates. This study contributes to a better understanding of substrate specificity in SLC35A1 and SLC35A2 and has implications for disease treatments.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Immunology
Su-bin Hwang, Ramachandran Chelliah, Ji Eun Kang, Momna Rubab, Eric Banan-MwineDaliri, Fazle Elahi, Deog-Hwan Oh
Summary: STEC is a global foodborne bacterial pathogen that can cause severe diarrhea and other critical illnesses, mainly through inflammation caused by Shiga toxins. There is currently no widely accepted treatment for STEC infections, and further research is needed.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Graca Pinto, Marta Sampaio, Oscar Dias, Carina Almeida, Joana Azeredo, Hugo Oliveira
Summary: This study analyzed 179 complete genomes of Shiga toxin-producing Escherichia coli (STEC) in terms of serotypes, prophage coding regions, and stx gene variants, revealing a high genetic diversity and the prevalence of non-O157 serotypes. Most STEC genomes contain a single stx gene, with some strains harboring two, three, or four stx genes, and stx2 being the most common variant. The study also identified four clusters, three subclusters, and one singleton of Stx phages, showing a diverse genetic structure with a strong selective pressure to maintain the location of stx genes close to lytic cassettes.
Article
Chemistry, Organic
Samir Ghosh, Sharmeen Nishat, Peter R. Andreana
JOURNAL OF ORGANIC CHEMISTRY
(2016)
Article
Chemistry, Organic
Pradheep Eradi, Samir Ghosh, Peter R. Andreana
Article
Chemistry, Medicinal
Samir Ghosh, Pallavi Tiwari, Shashi Pandey, Anup Kumar Misra, Vinita Chaturvedi, Anil Gaikwad, Shalini Bhatnagar, Sudhir Sinha
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2008)
Article
Chemistry, Medicinal
Samir Ghosh, Anup Kumar Misra, Gitika Bhatia, M. M. Khan, A. K. Khanna
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2009)
Article
Biochemistry & Molecular Biology
Samir Ghosh, Anup Kumar Misra
JOURNAL OF CARBOHYDRATE CHEMISTRY
(2009)
Article
Chemistry, Organic
Shashi Pandey, Samir Ghosh, Anup Kumar Misra
SYNTHESIS-STUTTGART
(2009)
Article
Chemistry, Inorganic & Nuclear
Samir Ghosh, Anup Kumar Misra
TETRAHEDRON-ASYMMETRY
(2009)
Article
Chemistry, Inorganic & Nuclear
Samir Ghosh, Anup Kumar Misra
TETRAHEDRON-ASYMMETRY
(2010)
Article
Chemistry, Inorganic & Nuclear
Samir Ghosh, Anup Kumar Misra
TETRAHEDRON-ASYMMETRY
(2010)
Article
Biochemistry & Molecular Biology
F. Hossain, S. Nishat, S. Ghosh, S. Boga, G. T. Hymel, P. R. Andreana
JOURNAL OF CARBOHYDRATE CHEMISTRY
(2020)
Article
Chemistry, Medicinal
Brendan T. Freitas, Daniil A. Ahiadorme, Rahul S. Bagul, Ian A. Durie, Samir Ghosh, Jarvis Hill, Naomi E. Kramer, Jackelyn Murray, Brady M. O'Boyle, Emmanuel Onobun, Michael G. Pirrone, Justin D. Shepard, Suzanne Enos, Yagya P. Subedi, Kapil Upadhyaya, Ralph A. Tripp, Brian S. Cummings, David Crich, Scott D. Pegan
Summary: In the past 20 years, both severe acute respiratory syndrome coronavirus-1 and severe acute respiratory syndrome coronavirus-2 have caused zoonotic outbreaks in humans. The PLpro enzyme from a subgroup 2b bat coronavirus has been studied to identify structural features and substrate specificity. Based on this, 30 novel noncovalent inhibitors for subgroup 2b PLpro enzymes were designed, providing new directions for antiviral development against this group of coronaviruses.
ACS INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Samir Ghosh, Kevin R. Trabbic, Mengchao Shi, Sharmeen Nishat, Pradheep Eradi, Kristopher A. Kleski, Peter R. Andreana
