期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 135, 期 3, 页码 403-411出版社
SPRINGER
DOI: 10.1007/s00432-008-0474-3
关键词
Stomach neoplasms; Mass spectrometry; Proteomics; Prognosis; S100P
类别
资金
- National Natural Science Foundation of China [30571807]
- Foundation of Beijing Municipal Committee of Science & Technology, China [D0905001040631]
The objective of this study was to identify differentially expressed proteins of advanced gastric cancer from patients with different prognosis using NanoLC-MS/MS (LTQ) (nanoflow liquid chromatography system interfaced with a linear ion trap LTQ mass spectrometer). Eight gastric cancer patients with relatively early TNM stage and survival time > 34 months were identified as good survival (group G), while the other eight with late stage and survival time < 15 months as poor survival (group P). The total protein of the tissue samples from each group was extracted and pooled together respectively. The resulting two protein mixtures were trypsin-digested and analyzed using NanoLC-MS/MS (LTQ). Database searches were done against NCBI non-redundant database and SWISS-PROT database and the identified proteins were classified through an online Web Gene Ontology Annotation Plot tool. Immunohistochemistry was used to verify candidate prognosis-related proteins. There were 284 and 213 proteins identified for group G and group P respectively. And 117 proteins were detected exclusively in group G and 46 proteins exclusively in group P. These protein markers function in calcium ion signaling pathway, cellular metabolism, cytoskeleton formation, stress reaction, etc. Among those, the down-regulated expression of S100P was verified to claim a poor clinical outcome of gastric cancer patients (P = 0.0375). The MS-based proteomics approach is efficient in identifying differentially expressed proteins in relation to prognosis of advanced gastric cancer patients. These differentially expressed proteins could be potential prognosis-related cancer markers and deserve further validation and functional study.
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