Article
Cell & Tissue Engineering
Johanna F. A. Husch, Talita Stessuk, Cezanne den Breejen, Manouk van den Boom, Sander C. G. Leeuwenburgh, Jeroen J. J. P. van den Beucken
Summary: In this study, a simple and reliable three-step method for human osteoclast generation from blood-derived precursor cells was described. Osteoclastogenesis was monitored longitudinally using nondestructive techniques, and the functionality and morphology of mature osteoclasts were confirmed after 14 days in culture. The use of combinatory staining of three markers is recommended to distinguish osteoclasts from undifferentiated mononuclear cells.
TISSUE ENGINEERING PART C-METHODS
(2021)
Article
Biochemistry & Molecular Biology
Anne Bernhardt, Jana Bacova, Uwe Gbureck, Michael Gelinsky
Summary: The study found that the effects of Cu2+ on osteoclasts varied depending on their differentiating or mature state. High TRAP activity does not necessarily correlate with high resorption capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Seong Cheol Kim, Hyeon Jeong Kim, Gi Eun Park, Ramesh Prasad Pandey, Jisun Lee, Jae Kyung Sohng, Yong Il Park
Summary: This study demonstrated that Tri inhibits osteoclastic cell differentiation and bone resorption by down-regulating the expression of osteoclastogenic marker genes and signalling proteins, bone resorptive proteins, and by augmenting serum OPG/RANKL ratio, suggesting that Tri can be a novel anti-osteoporotic compound for treating senile and postmenopausal OP.
Review
Cell Biology
Sharon Russo, Federica Scotto di Carlo, Fernando Gianfrancesco
Summary: Osteoclasts play an important role in bone remodeling, but they are also involved in bone tumors and metastases. They increase the aggressiveness of primary tumors and promote metastasis of breast and pancreas cancer cells to the bone. Additionally, osteoclasts are responsible for reactivating dormant cancer cells in the bone marrow, leading to overt metastasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Bethan K. Davies, Andrew P. Hibbert, Scott J. Roberts, Helen C. Roberts, Jennifer C. Tickner, Gill Holdsworth, Timothy R. Arnett, Isabel R. Orriss
Summary: This study developed and validated an automated workflow using machine learning-based image analysis software to accurately quantify in vitro osteoclast cultures. The algorithm showed strong correlation with manual counting methods and accurately segmented osteoclasts. The method was successfully validated for detecting changes in osteoclast number with different treatments and exhibited advantages in reducing user variability and analysis time.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Article
Cell Biology
Hirohito Hirata, Asana Kamohara, Masatoshi Murayama, Kenichi Nishioka, Hiroaki Honda, Yasuteru Urano, Hidenobu Soejima, Shinya Oki, Toshio Kukita, Shunsuke Kawano, Masaaki Mawatari, Akiko Kukita
Summary: Bhlhe40 is a crucial regulator of osteoclast differentiation and function, and its deficiency leads to increased bone volume and reduced bone resorption activity. Analysis of potential target genes reveals their association with proton transport and intracellular vesicle acidification.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Biao Wu, Jie Shang, Shiyuan Lin, Ning Jiang, Baizhou Xing, Rong Peng, Xianghe Xu, Huading Lu
Summary: Increased bone resorption caused by excessive osteoclasts is the main cause of osteoporosis. The expression of RILP, a protein, is induced by receptor activator of NF-KB ligand, and its inhibition decreases the number and function of osteoclasts. Inhibition of RILP also reduces preosteoclast migration and bone resorption. The study suggests that RILP plays a crucial role in osteoclast formation and bone resorption, and could be a therapeutic target for bone diseases caused by excessive osteoclasts.
LABORATORY INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Perry C. Caviness, Dongzheng Gai, Oxana P. Lazarenko, Michael L. Blackburn, Fenghuang Zhan, Jin-Ran Chen
Summary: Osteoclasts derived from hematopoietic stem cells play a crucial role in bone resorption. Identifying new molecules that can epigenetically regulate osteoclastogenesis is essential for the development of novel treatments for bone deterioration disorders like osteoporosis. The protein Ezh2 has been found to be associated with the epigenetic regulation of various cellular processes, but its role in bone cell development is still not fully understood.
Article
Biochemistry & Molecular Biology
Hye Jung Ihn, Jiwon Lim, Kiryeong Kim, Sang-Hyeon Nam, Soomin Lim, Su Jeong Lee, Jong-Sup Bae, Tae Hoon Kim, Jung-Eun Kim, Moon-Chang Baek, Yong Chul Bae, Eui Kyun Park
Summary: Ciclopirox effectively inhibits osteoclast differentiation and bone resorption both in vitro and in vivo, making it a potential candidate for preventing osteoporosis and other osteoclast-related bone diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pedro P. C. de Souza, Petra Henning, Ulf H. Lerner
Summary: Oncostatin M (OSM) is a potent stimulator of osteoclast formation and plays a significant role in bone remodeling by inducing RANKL in osteoblasts. Gene deletion of Osmr in mice leads to decreased osteoclast numbers and increased trabecular bone thickness. OSM can promote osteoblast differentiation and mineralization, but in vivo administration in adult animals does not significantly increase osteoclast formation due to the negative feedback loop through the osteoclastogenesis inhibitor WNT16.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Hongkun Li, Yilin Li, Jinghua Zou, Yanran Yang, Ruiqi Han, Jun Zhang
Summary: Sinomenine can inhibit tooth movement and reduce root resorption in rats while exerting a positive effect on periodontal tissue, as well as promoting osteogenesis of periodontal ligament stem cells.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Endocrinology & Metabolism
Vincent Everts, Ineke D. C. Jansen, Teun J. de Vries
Summary: This short review discusses the unique features of osteoclasts that enable them to resorb mineralized tissues.
Article
Endocrinology & Metabolism
Jing-yi Xue, Shiro Ikegawa, Long Guo
Summary: SLC4A2 belongs to the SLC4 family of anion exchangers and regulates Cl- and HCO3- exchange, as well as intra- and extra-cellular pH, chloride concentration, and cell volume. It is involved in gastric acid secretion, spermatogenesis, and osteoclastogenesis. Deficiency in SLC4A2 has been found to cause a new type of autosomal recessive osteopetrosis. This review discusses the functions of SLC4A2, particularly in osteoclasts, and contributes to understanding the pathomechanism of SLC4A2-associated osteopetrosis.
Article
Chemistry, Medicinal
Yifeng Shi, Lin Ye, Shiwei Shen, Tianchen Qian, Youjin Pan, Yuhan Jiang, Jinghao Lin, Chen Liu, Yaosen Wu, Xiangyang Wang, Jiake Xu, Haiming Jin
Summary: The study demonstrated that morin can inhibit osteoclast formation through various pathways and attenuate bone loss induced by ovariectomy. This suggests that morin may be a potential therapeutic agent for postmenopausal osteoporosis treatment.
PHYTOTHERAPY RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Ling Ji, Xinyi Li, Shushu He, Song Chen
Summary: This review introduces the pathways and related microRNAs involved in osteoclastogenesis, discusses the clinical potential of targeting microRNAs for the treatment of osteoclast-mediated bone resorption, and the challenges of avoiding potential side effects and producing efficient delivery methods.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
