Article
Oncology
Takuya Wada, Katsutoshi Sugimoto, Kentaro Sakamaki, Hiroshi Takahashi, Tatsuya Kakegawa, Yusuke Tomita, Masakazu Abe, Yu Yoshimasu, Hirohito Takeuchi, Takao Itoi
Summary: This study compared the outcomes of radiofrequency ablation (RFA), microwave ablation (MWA), and irreversible electroporation (IRE) in treating early stage hepatocellular carcinoma (HCC). The results showed that IRE had a significantly lower 2-year local tumor progression (LTP) rate compared to RFA. There were no significant differences in LTP rates between IRE and MWA, and between RFA and MWA. Therefore, IRE may be a better first-line treatment option for small perivascular HCC.
Article
Medicine, General & Internal
Sahar Avazzadeh, Barry O'Brien, Ken Coffey, Martin O'Halloran, David Keane, Leo R. Quinlan
Summary: The study aimed to compare the optimal voltage thresholds for different cell types within the cardiovascular system and selective cell ablation. Results showed that neuronal and atrial cardiomyocyte lines had cell viabilities below 20% at 1000 V/cm, while cardiac fibroblasts showed an optimal threshold at 1250 V/cm.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Mohit K. Turagam, Petr Neuzil, Boris Schmidt, Tobias Reichlin, Kars Neven, Andreas Metzner, Jim Hansen, Yuri Blaauw, Philippe Maury, Thomas Arentz, Philipp Sommer, Ante Anic, Frederic Anselme, Serge Boveda, Tom Deneke, Stephan Willems, Pepijn van der Voort, Roland Tilz, Moritoshi Funasako, Daniel Scherr, Reza Wakili, Daniel Steven, Josef Kautzner, Johan Vijgen, Pierre Jais, Jan Petru, Julian Chun, Laurent Roten, Anna Fueting, Marc D. Lemoine, Martin Ruwald, Bart A. Mulder, Anne Rollin, Heiko Lehrmann, Thomas Fink, Zrinka Jurisic, Corentin Chaumont, Raquel Adelino, Karin Nentwich, Melanie Gunawardene, Alexandre Ouss, Christian-Hendrik Heeger, Martin Manninger, Jan-Eric Bohnen, Arian Sultan, Petr Peichl, Pieter Koopman, Nicolas Derval, Thomas Kueffer, Vivek Y. Reddy
Summary: This study compared the differences in clinical outcomes between male and female patients undergoing pulsed field ablation (PFA) for atrial fibrillation (AF). The results showed that there were no significant sex differences in clinical effectiveness or safety events after PFA for AF.
Article
Chemistry, Multidisciplinary
Pierre Agnass, Krijn P. van Lienden, Thomas M. van Gulik, Marc G. Besselink, Johannes Crezee, H. Petra Kok
Summary: The Electric-Potential Estimation (EPE) method accurately predicts the potential distribution near cylindrical electrodes, regardless of their size, position, and orientation. This method can significantly shorten the simulation time of treatment planning and improve computational efficiency.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Seung Jeong, Hongbae Kim, Junhyung Park, Ki Woo Kim, Sung Bo Sim, Jong Hoon Chung
Summary: The study utilized TTC staining to evaluate the IRE ablated area in a potato model, demonstrating that TTC staining accurately delineated the IRE ablated area and provided a more rapid evaluation method.
SCIENTIFIC REPORTS
(2021)
Review
Immunology
Dishiwen Liu, Yajia Li, Qingyan Zhao
Summary: Atrial fibrillation (AF) is a serious healthcare burden with high morbidity and complications. Catheter ablation, although curable, currently has a low long-term success rate. Inflammation and fibrosis play significant roles in AF progression, and the artificial inflammatory response caused by catheter ablation may impact long-term prognosis. This review focuses on the different types of inflammatory cell death induced by various ablation techniques, as well as the structural and electrical remodeling caused by inflammation and its association with AF recurrence after catheter ablation. Pulsed-field ablation shows promise as a revolutionary innovation with faster, safer, better tissue selectivity, and less inflammatory response.
JOURNAL OF INFLAMMATION RESEARCH
(2023)
Review
Radiology, Nuclear Medicine & Medical Imaging
Pankaj Gupta, Muniraju Maralakunte, Sathya Sagar, Praveen Kumar-M, Harish Bhujade, Sreedhara B. Chaluvashetty, Naveen Kalra
Summary: A systematic review of studies on irreversible electroporation (IRE) for liver malignancies showed that IRE is associated with favorable overall survival (OS) and progression-free survival (PFS), despite a high overall complication rate, with most complications being minor.
EUROPEAN RADIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
P. Briz, B. Lopez-Alonso, H. Sarnago, J. M. Burdio, O. Lucia
Summary: Electroporation is the increase in cell membrane permeability when exposed to high pulsed electric fields. This phenomenon can be used in tumor ablation therapies in a clinical setting. In this study, a pre-treatment tumor location method was developed to precisely target the therapy by using impedance measurements and artificial neural networks.
BIOELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Hongmei Liu, Yajun Zhao, Chenguo Yao, Eva M. Schmelz, Rafael Davalos
Summary: The study demonstrates that nsPEFs may have differential effects on tumor cells at different disease stages, preferentially targeting highly aggressive and malignant ovarian cancer cells over benign counterparts. This provides an experimental basis for future electrical treatments of malignant tumors.
BIOELECTROCHEMISTRY
(2021)
Article
Oncology
Mirella Tanori, Arianna Casciati, Alessandro Zambotti, Rosanna Pinto, Isabella Gianlorenzi, Alessandro Pannicelli, Paola Giardullo, Barbara Benassi, Carmela Marino, Mariateresa Mancuso, Caterina Merla
Summary: Using microsecond pulsed electric fields can specifically target cancer stem cells, inducing permeabilization and apoptosis in medulloblastoma cancer stem cells while generating reactive oxygen species and temporary cell cycle arrest in surviving cells. In vivo validation shows that electric pulse pre-exposure combined with ionizing irradiation can effectively inhibit tumor growth.
INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
(2021)
Article
Cardiac & Cardiovascular Systems
Atul E. Verma, David V. Haines, Lucas Boersma, Nitesh Sood, Andrea E. Natale, Francis Marchlinski, Hugh Calkins, Prashanthan L. Sanders, Douglas Packer, Karl-Heinz Kuck, Gerhard Hindricks, Birce Onal, Jeffrey Cerkvenik, Hiroshi B. Tada, David DeLurgio, PULSED AF Investigators
Summary: Pulsed field ablation is an effective and safe method for treating patients with atrial fibrillation. It has comparable effectiveness to traditional catheter ablation but with a lower rate of complications.
Review
Medicine, General & Internal
Maria Beatrice Bocchi, Cesare Meschini, Silvia Pietramala, Andrea Perna, Maria Serena Oliva, Maria Rosaria Matrangolo, Antonio Ziranu, Giulio Maccauro, Raffaele Vitiello
Summary: This review evaluated the effectiveness of electrochemotherapy in the treatment of bone metastases. The findings suggest that electrochemotherapy can be considered as a minimally invasive and tissue-sparing treatment for patients who have no other alternatives for tumor control and improvement in quality of life.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cardiac & Cardiovascular Systems
Boris Schmidt, Stefano Bordignon, Kars Neven, Tobias Reichlin, Yuri Blaauw, Jim Hansen, Raquel Adelino, Alexandre Ouss, Anna Fueting, Laurent Roten, Bart A. Mulder, Martin H. Ruwald, Roberto Mene, Pepijn van der Voort, Nico Reinsch, Thomas Kueffer, Serge Boveda, Elizabeth M. Albrecht, Christopher W. Schneider, Kyoung Ryul Julian Chun
Summary: The EU-PORIA registry confirms the safety and efficacy of pulsed field ablation for pulmonary vein isolation in patients with atrial fibrillation. The use of the pentaspline, multi-electrode PFA catheter shows excellent results and a short learning curve.
Article
Cardiac & Cardiovascular Systems
Yonatan Moshkovits, Dvora Grynberg, Eyal Heller, Leonid Maizels, Elad Maor
Summary: This study aimed to compare the effects of different HF-IRE protocols on cardiac and other tissues. The results showed that high-frequency electroporation protocols had larger lesions in myocardial tissue compared to neural and vascular tissues. Low-amplitude HF-IRE protocols did not cause any damage to neural or vascular tissues. Further research is needed to identify the tissue-specific thresholds in other tissues.
Article
Cardiac & Cardiovascular Systems
Anna Fueting, Nico Reinsch, Dennis Hoewel, Lenny Brokkaar, Gilbert Rahe, Kars Neven
Summary: PVI using PFA for PAF in a 'real-world' setting appears to be safe and feasible in this small patient cohort. Procedure times are homogeneous, and LA dwell time is short. Atrial ablation lines can easily be created. Unintentional ablation of atrial tissue can occur, accurate catheter alignment to the PV ostium and axis should be ensured.
Article
Orthopedics
Giuseppe Filardo, Matilde Tschon, Francesco Perdisa, Silvia Brogini, Carola Cavallo, Giovanna Desando, Gianluca Giavaresi, Brunella Grigolo, Lucia Martini, Nicolo Nicoli Aldini, Alice Roffi, Milena Fini, Elizaveta Kon
Summary: The study compared three procedures for treating osteoarthritis using adipose-derived cells. Mechanical micro-fragmentation (MF) showed the most promising results in protecting the articular surface from OA processes.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2022)
Article
Biochemistry & Molecular Biology
Matilde Tschon, Elisa Boanini, Maria Sartori, Francesca Salamanna, Silvia Panzavolta, Adriana Bigi, Milena Fini
Summary: In this study, zoledronate functionalized hydroxyapatite nanocrystals (HAZOL) were found to promote the adhesion, viability, metabolic activity, and osteogenic commitment of human bone marrow derived mesenchymal stromal cells (hMSCs). The scaffolds supported cell growth and enhanced osteogenic differentiation and gene expression of hMSCs. These results suggest that zoledronate functionalized scaffolds may have a positive influence on bone fracture healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Gianmarco Tuzzato, Roberta Laranga, Federico Ostetto, Elisa Bubbico, Giulio Vara, Giuseppe Bianchi
Summary: This study analyzed the history and treatment outcomes of localized, high-grade myxoid liposarcoma (MLS) of the extremities. The results showed that patients with tumors in the extremities had a good overall survival rate and local recurrence-free survival rate. Tumor sites and surgical margins were not significant risk factors for survival, while tumor size was a significant predictor of metastasis-free survival and disease-free survival.
Article
Oncology
Alessandra De Feo, Laura Pazzaglia, Lisa Ciuffarin, Fabio Mangiagli, Michela Pasello, Elisa Simonetti, Evelin Pellegrini, Cristina Ferrari, Giuseppe Bianchi, Benedetta Spazzoli, Katia Scotlandi
Summary: Ewing's sarcoma, a common pediatric bone tumor, relies on genetic and epigenetic alterations that induce gene expression reprogramming. The study identifies miR-214-3p as a common mediator of EWS-FLI1 and CD99, and its restoration inhibits tumor cell growth and migration by repressing HMGA1 expression.
Article
Biology
Mattia Di Bartolomeo, Francesco Cavani, Arrigo Pellacani, Alexis Grande, Roberta Salvatori, Luigi Chiarini, Riccardo Nocini, Alexandre Anesi
Summary: Pulsed electromagnetic fields (PEMFs) are a type of biophysical stimulation that have shown promising results in improving bone regeneration and preventing bone loss. However, there is limited understanding of the underlying biological mechanisms and the effects on bone healing. The variability in the characteristics of electromagnetic fields and the responsiveness of cells involved in bone healing pose challenges in drawing definitive conclusions. This review summarizes the current research on PEMF stimulation in animal models with bone impairment, highlighting the need for standardized experimental guidelines and further controlled trials.
Review
Biochemistry & Molecular Biology
Chiara Delbaldo, Matilde Tschon, Lucia Martini, Milena Fini, Giorgia Codispoti
Summary: This systematic review investigates the latest advancements in preclinical efficacy studies applying nanotechnologies to hydrogels in osteoarthritis (OA) models. The studies found that nanogels do not impact cell viability and can reduce inflammation and prevent articular cartilage damage. However, more research and technical exploration are still needed for the translation of nanotechnologies into an effective therapy for OA patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biology
Alexandre Anesi, Francesco Cavani
Article
Chemistry, Multidisciplinary
Giuseppe Bianchi, Maria Grazia Benedetti, Roberta Bardelli, Daniela Platano, Roberta Laranga, Gianmarco Tuzzato
Summary: This case study examines scapula reconstruction using three-dimensional printing in a patient with low-grade osteosarcoma. Custom prosthetic reconstruction through three-dimensional printing proved to be a valid alternative, offering excellent functional and aesthetic results in oncological cases.
APPLIED SCIENCES-BASEL
(2023)
Article
Oncology
Giuseppe Bianchi, Roberta Laranga, Paolo Spinnato, Federico Ostetto, Elisa Bubbico, Alberto Righi, Davide Maria Donati
Summary: Biopsy plays a crucial role in diagnosing myxoid liposarcoma (MLs) due to the significance of histological grade in determining the appropriate treatment. This retrospective study aimed to assess the accuracy of Tru-cut(R) biopsy (TCB) in diagnosing MLs and its impact on patient survival in case of misdiagnosis. The study found that TCB diagnosis may differ from surgical specimens, with a histological grade concordance rate of 64% (Kappa 0.30). Neoadjuvant treatments were associated with pathological downgrading, but misdiagnosis did not affect prognosis. In patients not treated with neoadjuvant therapy, TCB showed a sensitivity of 57% and specificity of 100%.
Article
Biochemistry & Molecular Biology
Marzia Ferretti, Francesco Cavani, Vincenza Rita Lo Vasco, Marta Checchi, Serena Truocchio, Pierpaola Davalli, Chiara Frassineti, Federica Rizzi, Carla Palumbo
Summary: This study investigated the effects of a Mg-deficient diet on the bone of 8-weeks-old C57BL/6J male mice using a histological approach. The results showed that mice receiving the Mg-deficient diet had reduced body weight gain and serum Mg concentration, while the expression of kidney magnesiotropic genes tended to increase, indicating an adaptive response to Mg depletion. However, there were no significant differences in bone density and growth plate thickness, which partially differed from previous studies and might be attributed to experimental protocol differences or genetic factors.
Article
Biology
Fabio Vita, Gianmarco Tuzzato, Davide Pederiva, Giuseppe Bianchi, Augusto Marcuzzi, Roberto Adani, Paolo Spinnato, Marco Miceli, Danilo Donati, Marco Manzetti, Federico Pilla, Cesare Faldini
Summary: This study compared two treatment methods for osteoid osteoma of the hand: surgical excision and radio-frequency ablation (RFA). The results suggest that RFA can be an alternative to surgery, providing rapid pain relief and a swift return to work.
Article
Biochemistry & Molecular Biology
Francesca Salamanna, Deyanira Contartese, Veronica Borsari, Stefania Pagani, Maria Sartori, Matilde Tschon, Cristiana Griffoni, Gianluca Giavaresi, Giuseppe Tedesco, Giovanni Barbanti Brodano, Alessandro Gasbarrini, Milena Fini
Summary: Recently, our group studied the use of vertebral bone marrow (vBMA) clot as a cell therapy for spinal fusion. We compared the properties of vBMA clots and derived vertebral mesenchymal stem cells (MSCs) from female and male patients who underwent spinal fusion procedures and received vBMA clot treatment. The results showed that vBMA clots and MSCs from males had higher expression of growth factors (GFs) and greater osteogenic and chondrogenic potential compared to females. The findings also revealed differences in the gene expression profiles of MSCs derived from vBMA clots between female and male donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Andrea Sambri, Michele Fiore, Matteo Rottoli, Giuseppe Bianchi, Marco Pignatti, Marta Bortoli, Amelio Ercolino, Stefano Ancetti, Anna Myriam Perrone, Pierandrea De Iaco, Riccardo Cipriani, Eugenio Brunocilla, Davide Maria Donati, Mauro Gargiulo, Gilberto Poggioli, Massimiliano De Paolis
Summary: The pelvic anatomy presents challenges to orthopedic surgeons due to the large size and close proximity to vital structures of pelvic sarcomas. This study reports a systematic multidisciplinary surgical approach to treating pelvic sarcomas, in which 17 patients were included and treated using a combination of expertise from orthopedic oncology and other surgical specialties. Hindquarter amputation was performed on seven patients, while excision of the tumor was done for 10 patients. Bone defects were reconstructed in six patients using a custom-made 3D-printed pelvic prosthesis. Thirteen patients experienced complications. Well-organized multidisciplinary collaborations are crucial for managing pelvic sarcoma patients and should be conducted in specialized centers. A multidisciplinary surgical approach is essential for achieving successful outcomes and adequate margins.
Article
Engineering, Biomedical
Elisa Boanini, Stefania Pagani, Matilde Tschon, Katia Rubini, Milena Fini, Adriana Bigi
Summary: This study investigated the influence of strontium substitution on the structures of monetite and brushite, and its effect on bone cell response. The results showed that strontium substitution caused changes in cell parameters and morphology, and the release of ions was greater in brushite compared to monetite. The study also found that brushite materials had a more pronounced effect on osteoblast differentiation and osteoclast inhibition.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Oncology
Laura Campanacci, Luca Cevolani, Francesca De Terlizzi, Laura Saenz, Nikolin Ali, Giuseppe Bianchi, Davide Maria Donati
Summary: Electrochemotherapy (ECT) is an effective and feasible treatment for bone metastases, reducing pain and improving quality of life.
