Article
Immunology
Tomabu Adjobimey, Achim Hoerauf
Summary: This study investigates the link between total IgG-N-glycosylation patterns and the physiopathology of human lymphatic filariasis. The results reveal distinct N-linked IgG glycan profiles in different clinical groups, suggesting a correlation between IgG galactosylation and sialylation and chronic pathology, and agalactosylation and putative immunity. These findings emphasize the importance of N-glycosylation in lymphatic filariasis and its potential for developing new therapeutic formulations.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Cell Biology
Xinqing Zhu, Abdullah Al-Danakh, Lin Zhang, Xiaoxin Sun, Yuli Jian, Haotian Wu, Dan Feng, Shujing Wang, Deyong Yang
Summary: Renal cell carcinoma is a common malignant tumor of the urinary system. Understanding the role of glycosylation in RCC may lead to the identification of novel targets and molecular markers for diagnosis and therapy.
Article
Immunology
Marie Morel, Pierre Pochard, Wiam Echchih, Maryvonne Dueymes, Cristina Bagacean, Sandrine Jousse-Joulin, Valerie Devauchelle-Pensec, Divi Cornec, Christophe Jamin, Jacques-Olivier Pers, Anne Bordron
Summary: This study investigated the glycosylation of B cell subsets in systemic lupus erythematosus (SLE), primary Sjogren's syndrome (pSS), and healthy donors. The findings showed specific alterations in glycosylation profiles of B cells in both diseases, indicating that defective glycosylation may contribute to the development of pathogenic autoantibodies. The study also extended the glycan profile to serum proteins and immunoglobulins, further supporting the importance of glycosylation in autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Oncology
Chengcheng Liao, Qian Wang, Jiaxing An, Jie Chen, Xiaolan Li, Qian Long, Linlin Xiao, Xiaoyan Guan, Jianguo Liu
Summary: The interaction of CD44, a non-kinase transmembrane glycoprotein, with ligands like hyaluronic acid plays a crucial role in tumor occurrence and development. Changes in CD44 glycosylation can regulate multiple signaling pathways, impacting the tumor microenvironment and cell fate. However, our current understanding of CD44 glycosylation and its effects on tumors is limited.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Katarzyna Solkiewicz, Hubert Krotkiewski, Marcin Jedryka, Ewa M. Kratz
Summary: Analysis of serum IgG glycosylation can provide valuable supplementary parameters for the diagnosis of endometriosis, helping to differentiate women with endometriosis from healthy women. The results suggest that IgG sialylation and galactosylation/agalactosylation degree can serve as indicators for assessing the risk of endometriosis.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Chiara De Vellis, Silvia Pietrobono, Barbara Stecca
Summary: Malignant melanoma, originating from the malignant transformation of melanocytes, is the most aggressive form of skin cancer. Aberrant glycosylation, including sialylation, fucosylation, and N- and I-glycan branching, is considered a hallmark of cancer and plays a role in promoting melanoma progression through activation of invasion and metastasis, immune evasion, and cell proliferation.
Article
Biotechnology & Applied Microbiology
Anuja Prabhu, Dhanasekaran Shanmugam, Mugdha Gadgil
Summary: This study demonstrates the ability to predictably modulate the glycoform profile of recombinant IgG by engineering nucleotide sugar synthesis pathways in CHO cells. By knocking out specific enzymes, the researchers were able to narrow down the array of N-glycans and control the galactosylation and fucosylation levels of the recombinant IgG.
METABOLIC ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Henrique O. Duarte, Joana G. Rodrigues, Catarina Gomes, Paul J. Hensbergen, Agnes L. Hipgrave Ederveen, Arnoud H. de Ru, Stefan Mereiter, Antonio Polonia, Elisabete Fernandes, Jose A. Ferreira, Peter A. van Veelen, Lucio L. Santos, Manfred Wuhrer, Joana Gomes, Celso A. Reis
Summary: The resistance of ErbB2-positive gastric cancer cells to trastuzumab is actively modulated by ST6Gal1-mediated aberrant alpha 2,6-sialylation, which reshapes the glycomes and sensitizes the cells to trastuzumab-induced cytotoxicity.
Article
Multidisciplinary Sciences
Iwona Wojcik, David E. Schmidt, Lisa A. de Neef, Minke A. E. Rab, Bob Meek, Okke de Weerdt, Manfred Wuhrer, C. Ellen van der Schoot, Jaap J. Zwaginga, Masja de Haas, David Falck, Gestur Vidarsson
Summary: The spleen plays a crucial role in adaptive immune responses to viral infections and alloantigens by hosting a variety of immune cell populations. Splenectomy leads to an increase in IgG-Fc fucosylation, suggesting that the spleen may play a role in generating afucosylated IgG responses. Unexpectedly, ITP patients show a decrease in total IgG-Fc fucosylation compared to healthy controls, pointing to a potential role of the spleen in regulating afucosylated IgG responses.
SCIENTIFIC REPORTS
(2021)
Article
Nutrition & Dietetics
Tyler Kim, Yixuan Xie, Qiongyu Li, Virginia M. Artegoitia, Carlito B. Lebrilla, Nancy L. Keim, Sean H. Adams, Sridevi Krishnan
Summary: The study reveals that glycosylation of proteins may be influenced by dietary patterns, with a healthier diet pattern associated with higher sialylation levels.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Review
Biochemistry & Molecular Biology
Kayla Bastian, Emma Scott, David J. Elliott, Jennifer Munkley
Summary: Aberrant glycosylation, particularly altered core fucosylation driven by FUT8, is a universal feature of cancer cells impacting tumor progression. Upregulated FUT8 plays critical roles in immune evasion and cancer development, outlining potential opportunities for future therapeutics targeting FUT8.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Pirjo Nummela, Annamari Heiskanen, Soili Kytola, Caj Haglund, Anna Lepisto, Tero Satomaa, Ari Ristimaki
Summary: Pseudomyxoma peritonei (PMP) is a highly mucinous adenocarcinoma originating most commonly from the appendix, and alterations in the sialic acid linkage pattern in PMP tumors may play a role in the dissemination and progression of the disease.
Article
Biochemistry & Molecular Biology
Balazs Gyebrovszki, Andras Acs, Daniel Szabo, Felicia Auer, Soma Novozanszki, Bernadette Rojkovich, Anna Magyar, Ferenc Hudecz, Karoly Vekey, Laszlo Drahos, Gabriella Sarmay
Summary: This study aims to analyze the glycosylation patterns of ACPA and control IgG, and evaluate their ability to induce TNFα production and their correlation with inflammatory markers in RA. The study found that the sialylation and galactosylation levels of ACPA are negatively correlated with inflammation-related parameters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Sri R. Madabhushi, Alexei A. Podtelezhnikov, Nicholas Murgolo, Sen Xu, Henry Lin
Summary: The study found that different treatments have distinct complex mechanisms influencing the overall glycan levels of a monoclonal antibody, impacting the desired productivity and product quality.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Medicine, General & Internal
Xiaoni Meng, Fei Wang, Xiangyang Gao, Biyan Wang, Xizhu Xu, Youxin Wang, Wei Wang, Qiang Zeng
Summary: This study aims to explore the relationship between suboptimal health status (SHS) and type 2 diabetes mellitus (T2DM) by examining the characteristics of IgG N-glycans. The study found significant associations between IgG N-glycan traits and T2DM. Combining IgG N-glycans with clinical features, a combined model was developed that could better differentiate between T2DM patients and healthy individuals. The findings suggest that early detection of T2DM risk individuals and personalized prevention and treatment can be achieved through the detection of IgG N-glycans.
Article
Biotechnology & Applied Microbiology
Ce Shi, Sebastian Vogg, Dong-Qiang Lin, Mattia Sponchioni, Massimo Morbidelli
Summary: A study developed a design procedure (DP) suitable for optimizing both batch frontal chromatography and Flow2 in terms of purity, yield, and productivity. Comparing the two operated at their optimal conditions showed that the Flow2 process demonstrated a more favorable Pareto front of yield and productivity at a specified purity, and exhibited improved robustness compared to the batch process.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Larissa Brosi, Eric Kuebler, Anna Weston, Patrick Romann, Sherin Panikulam, Lorin Dirscherl, Michael Gerspach, Cedric Giegelmann, Daniele Dolce, Marie-Eve Ueberschlag, Anna Melone, Frank Bantleon, Thomas K. Villiger, Christian-B Gerhold
Summary: The study demonstrates the recombinant expression of an extended receptor binding domain (RBD) of the SARS-CoV-2 Spike protein for a rapid immunoassay to detect RBD binding antibodies with high sensitivity and specificity. This test can also be used to monitor a successful immune response after vaccination with an RBD based vaccine, as current vaccines target the Spike RBD.
Article
Engineering, Chemical
Martina Jezkova, Petr Jelinek, Oskar Marelja, Dan Trunov, Marketa Jarosova, Zdenek Slouka, Miroslav Soos
Summary: Multicomponent hollow aggregates were prepared using an emulsion templating method adapted to microfluidic format, with IO and Au NPs in an inner inorganic shell covered with PCL NPs. Smaller aggregates were achieved compared to immiscible phases due to diffusion controlled shrinkage, with surface properties controlled by the mobilities and affinities of IO, Au, and PCL NPs. The biocompatible surface layer and inorganic shell with core space for cargo loading make these constructs advantageous for medicinal applications.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Chemistry, Physical
Yannis Vasilopoulos, Jan Heyda, Jan Rohlicek, Eliska Skorepova, Vitek Zvonicek, Miroslav Soos
Summary: This study investigates the kinetics of desolvation in drug manufacturing, focusing on the interaction between solvents and active pharmaceutical ingredients (APIs). The results reveal the role of solvent diffusion and provide insights into the nanoscale interactions between the drug and solvent molecules during desolvation.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biotechnology & Applied Microbiology
Ismaele Fioretti, Thomas Mueller-Spaeth, Richard Weldon, Sebastian Vogg, Massimo Morbidelli, Mattia Sponchioni
Summary: Oligonucleotides (ONs) are a promising class of biotherapeutics in the biopharmaceutical industry due to their unique way of regulating gene expression and influencing protein synthesis. However, their production cost limits large-scale manufacturing, which can be alleviated through process intensification. In this study, an efficient and continuous chromatographic purification process for ONs was developed, improving productivity and buffer consumption compared to traditional batch processes.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Argyro Chatziadi, Eliska Skorepova, Jan Jirat, Jan Rohlicek, Miroslav Soos
Summary: In this study, we successfully synthesized three cocrystals, two solvates, and two coamorphous systems of sofosbuvir using slurry experiments, solution evaporation, and mechanochemistry. We characterized these multicomponent solid forms and determined the crystal structures of sofosbuvir with piperazine, dichloromethane, and chloroform. Our results provide valuable insights into the molecular interactions in these systems.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Pharmacology & Pharmacy
Dominik Svara, Barbora Koprivova, Tomas Picek, Petr Mikes, Anna Kluk, Miroslav Soos
Summary: The purpose of this work is to prepare nanofibrous orally dispersible films (ODFs) using needleless electrospinning. The lamination pressure affects the mechanical properties of ODFs but has no impact on the dissolution kinetics.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Patrick Romann, Jakub Kolar, Daniela Tobler, Christoph Herwig, Jean-Marc Bielser, Thomas K. Villiger
Summary: This study developed a systematic Raman calibration workflow for perfusion processes in biopharmaceutical processes. The method allows for fast and specific model calibration, and can be applied to monitor other analytes of interest.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Eliska Skorepova, Milos Vaclavik, Jan Jirat, Morgane Poupon, Ludeek Ridvan, Martin Babor, Miroslav Soos
Summary: The aim of this study was to investigate the solid forms of obeticholic acid and characterize their structures. Two possible solvated forms were discovered using X-ray powder diffraction, and further analysis showed that each solvate contains its respective solvent. The two series of solvates are isostructural, and single-crystal X-ray diffraction revealed that both forms are channel solvates.
CRYSTAL GROWTH & DESIGN
(2022)
Article
Chemistry, Multidisciplinary
Jan Jirat, Martin Babor, Ludek Ridvan, Eliska Skorepova, Michal Dusek, Miroslav Soos
Summary: By examining the structure-property relations of apremilast cocrystals and solvates, this study presents a unique and large dataset of multicomponent crystal forms, including previously unpublished forms. The high similarity in crystal packing among these forms makes it easier to evaluate the structure-property relations. Interestingly, a significant correlation was found between the intrinsic dissolution rate of the multicomponent forms and their solubility as well as the solubility of the guest molecules in the dissolution medium. This finding is particularly important for the design of multicomponent forms with desired properties.
Article
Chemistry, Multidisciplinary
Sreela Ramesh, Eliska Skorepova, Vaclav Eigner, Vaisakh Chelod Paingad, Vit Zvonicek, Petr Kuzel, Miroslav Soos
Summary: The aim of this study was to prepare new crystalline salts of cyamemazine, an antipsychotic drug, with dicarboxylic acids, and compare their thermal stabilities, dissolution properties, and structural features. Among the eight acids investigated, novel salts were obtained with four of them. This study provides the first-ever examination of the solid-state landscape and crystal structure of cyamemazine.
CRYSTAL GROWTH & DESIGN
(2023)
Article
Thermodynamics
Pavel Krysa, Miroslav Soos
Summary: This study used computational fluid dynamics (CFD) to predict the breakage and coalescence of bubbles in gas-liquid systems and to predict the bubble size distribution (BSD). The CFD model was validated by comparing the simulation results with experimental data, and a characterization analysis of the system was performed.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2022)
Article
Biotechnology & Applied Microbiology
Patrick Romann, Jakub Kolar, Loic Chappuis, Christoph Herwig, Thomas K. Villiger, Jean-Marc Bielser
Summary: Bleed recycling is a novel method for increasing the yield of steady-state perfusion processes. By concentrating process bleed and selectively removing biomass, it allows for significant product saving. The use of inclined gravity settling as a bleed recycling technology showed similar efficiency to acoustic separation and had no negative impact on cell viabilities and product quality. With reduced system complexity and scalability, inclined gravity settling is the preferred technology for perfusion processes.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Georgia Koutentaki, Pavel Krysa, Dan Trunov, Tomas Pekarek, Marketa Pislova, Miroslav Soos
Summary: The properties of paracetamol particles coated with carnauba wax were investigated. Raman mapping technique was used to examine the thickness and homogeneity of the coating layer. The results showed that the wax existed in two forms on the surface of the particles, and the coating thickness had high variability. The ability of carnauba wax to decrease the dissolution rate of paracetamol was confirmed.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Patrick Romann, Sebastian Schneider, Daniela Tobler, Martin Jordan, Arnaud Perilleux, Jonathan Souquet, Christoph Herwig, Jean-Marc Bielser, Thomas K. Villiger
Summary: The Raman-controlled pyruvate feeding strategy stabilized metabolic activity and prevented product quality drifting in perfusion cell cultures.
BIOTECHNOLOGY JOURNAL
(2023)
