Review
Biochemistry & Molecular Biology
Mehdi Arbabi-Ghahroudi
Summary: Since the discovery of camelid heavy-chain antibodies in 1993, there has been great interest in the research and application of nanobodies. After years of effort, the first nanobody drug was approved in 2018-2019 for the treatment of rare blood clotting disorders. Despite intellectual property restrictions, nanobodies have the potential to become mainstream biotherapeutics in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Yasaman Asaadi, Fatemeh Fazlollahi Jouneghani, Sara Janani, Fatemeh Rahbarizadeh
Summary: By comparing the structure and properties of camelid antibodies (VHHs) and single-chain variable fragments (scFvs), it can be determined which one is more suitable for their respective therapeutic, diagnostic, and research applications.
BIOMARKER RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Mireille Elodie Tsitokana, Pierre-Andre Lafon, Laurent Prezeau, Jean-Philippe Pin, Philippe Rondard
Summary: Therapeutic antibodies for the treatment of central nervous system diseases have been extensively studied, resulting in approved monoclonal antibodies for brain disease therapies. Additionally, non-invasive antibody-based imaging approaches have been explored for biomarker detection in brain cancers. However, the low ability of antibodies to cross the blood-brain barrier has led to the investigation of single-domain antibodies (sdAbs) as an alternative. While the brain uptake of these small antibodies is initially low, there is a growing number of studies reporting brain-penetrating sdAbs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Irina A. Favorskaya, Dmitry V. Shcheblyakov, Ilias B. Esmagambetov, Inna V. Dolzhikova, Irina A. Alekseeva, Anastasia I. Korobkova, Daria V. Voronina, Ekaterina I. Ryabova, Artem A. Derkaev, Anna V. Kovyrshina, Anna A. Iliukhina, Andrey G. Botikov, Olga L. Voronina, Daria A. Egorova, Olga V. Zubkova, Natalia N. Ryzhova, Ekaterina I. Aksenova, Marina S. Kunda, Denis Y. Logunov, Boris S. Naroditsky, Alexandr L. Gintsburg
Summary: Virus-neutralizing antibodies are crucial in treating COVID-19, but the emergence of SARS-CoV-2 variants with reduced sensitivity to existing antibody therapies necessitates the development of more potent and broad-spectrum neutralizing antibodies. This study isolated a panel of single-domain antibodies that specifically bind to the receptor-binding domain of the SARS-CoV-2 S glycoprotein. By generating dimeric molecules from the selected antibodies, the researchers achieved a significant increase in neutralizing activity, with the most potent heterodimeric molecule effectively neutralizing all major SARS-CoV-2 variants of concern. These findings highlight the potential of this heterodimeric molecule as a promising candidate for COVID-19 treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Biochemistry & Molecular Biology
Clarissa A. Seidler, Janik Kokot, Monica L. Fernandez-Quintero, Klaus R. Liedl
Summary: In this study, the conformational diversity of four VHH variants and their binding to hen egg-white lysozyme (HEL) were investigated. It was found that with an increase in affinity, the stability of the VHH structure increased and the residues contributing to antigen binding underwent significant changes. In addition, it was observed that the investigated nanobodies followed the conformational selection paradigm, with the binding competent conformation pre-existing within the structural ensembles without the presence of the antigen.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Oncology
Elisa C. Toffoli, Abdolkarim Sheikhi, Roeland Lameris, Lisa A. King, Amanda van Vliet, Bruce Walcheck, Henk M. W. Verheul, Jan Spanholtz, Jurriaan Tuynman, Tanja D. de Gruijl, Hans J. van der Vliet
Summary: A novel bispecific single domain antibody (VHH) targeting CD16 on NK cells and EGFR on tumor cells enhanced NK cell activation and cytotoxicity, showing potential for cancer therapy. This approach demonstrated efficacy in controlling tumor growth in colorectal cancer cell co-cultures, suggesting promise for further exploration in NK cell-based therapeutic approaches.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Charles A. Swofford, Sarah A. Nordeen, Lu Chen, Mahaam M. Desai, Joanna Chen, Stacy L. Springs, Thomas U. Schwartz, Anthony J. Sinskey
Summary: Misuse of antibiotics in aquaculture poses a public health risk and leads to the emergence of multiresistant pathogens. Rapid test kits are used to monitor food supply chains but lack accuracy. Researchers have developed single-domain antibodies that can improve the reliability and shelf-life of these test kits for small molecule antibiotics.
Article
Biochemistry & Molecular Biology
Charles A. Swofford, Sarah A. Nordeen, Lu Chen, Mahaam M. Desai, Joanna Chen, Stacy L. Springs, Thomas U. Schwartz, Anthony J. Sinskey
Summary: Antibiotics in aquaculture are important for preventing bacterial infections in fish, but their misuse poses a public health risk and contributes to the unintentional creation of multiresistant pathogens. Current rapid test kits for these drugs lack high accuracy, but discovering and engineering single-domain antibodies (VHHs) can improve the reliability and shelf-life of these test kits. By developing a novel anti-chloramphenicol VHH with improved binding affinity, the structure of VHH-hapten interactions provides insights for future engineering of VHHs against additional targets.
Article
Chemistry, Multidisciplinary
Xiaodie Yuan, Xia Zhao, Yuan Lin, Zhaohui Su
Summary: This study synthesized PDA nanoparticles of various sizes and introduced different surface groups to investigate their penetration and accumulation abilities in biofilms. The PDA nanoparticles of approximately 60 nm size showed higher penetration and accumulation abilities, and the positively charged amine groups were more effective in enhancing the interactions with the biofilm compared to carboxyl or methoxy groups. The PDA60 nanoparticles with amine surface groups also exhibited good photothermal properties.
Article
Infectious Diseases
Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stephane Gerard, Fany Reffuveille
Summary: The combination of non-antimicrobial molecules with antibiotics can significantly reduce bacterial biofilm formation, potentially offering a solution to combat antibiotic resistance emergence.
Article
Biotechnology & Applied Microbiology
Roeland L. Berendsen, Marcel C. van Verk, Ioannis A. Stringlis, Christos Zamioudis, Jan Tommassen, Corne M. J. Pieterse, Peter A. H. M. Bakker
Article
Microbiology
Jesus Arenas, Vincent de Maat, Laura Caton, Massis Krekorian, Juan Cruz Herrero, Jan Tommassen
Article
Infectious Diseases
Sunita Paltansing, Margriet Kraakman, Ria van Boxtel, Ivo Kors, Els Wessels, Wil Goessens, Jan Tommassen, Alexandra Bernards
MICROBIAL DRUG RESISTANCE
(2015)
Article
Microbiology
Emily F. A. van 't Wout, Annemarie van Schadewijk, Ria van Boxtel, Lucy E. Dalton, Hanna J. Clarke, Jan Tommassen, Stefan J. Marciniak, Pieter S. Hiemstra
Article
Microbiology
Jesus Arenas, Sara Cano, Reindert Nijland, Verene van Dongen, Lucy Rutten, Arie van der Ende, Jan Tommassen
ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Florian Putker, Andreas Grutsch, Jan Tommassen, Martine P. Bos
JOURNAL OF BACTERIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Martine P. Bos, Jan Grijpstra, Ria Tommassen-van Boxtel, Jan Tommassen
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Biotechnology & Applied Microbiology
Hendrik Adams, Wannie M. Horrevoets, Simon M. Adema, Hannah E. V. Carr, Richard E. van Woerden, Margot Koster, Jan Tommassen
JOURNAL OF BIOTECHNOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Tessa Sinnige, Markus Weingarth, Marie Renault, Lindsay Baker, Jan Tommassen, Marc Baldus
JOURNAL OF MOLECULAR BIOLOGY
(2014)
Article
Microbiology
Virginie Roussel-Jazede, Jan Grijpstra, Vincent van Dam, Jan Tommassen, Peter van Ulsen
Article
Microbiology
Virginie Roussel-Jazede, Jesus Arenas, Jeroen D. Langereis, Jan Tommassen, Peter van Ulsen
Article
Biochemistry & Molecular Biology
Jesus Arenas, Reindert Nijland, Francisco J. Rodriguez, Tom N. P. Bosma, Jan Tommassen
MOLECULAR MICROBIOLOGY
(2013)
Article
Multidisciplinary Sciences
Elena B. Volokhina, Jan Grijpstra, Frank Beckers, Erika Lindh, Viviane Robert, Jan Tommassen, Martine P. Bos
Review
Microbiology
Florian Putker, Martine P. Bos, Jan Tommassen
FEMS MICROBIOLOGY REVIEWS
(2015)