Article
Biotechnology & Applied Microbiology
Yan Wang, Hengchang Li, Yu Liu, Mengyu Zhou, Mingzhu Ding, Yingjin Yuan
Summary: This study used systems biology analysis to construct a synthetic microbial consortium for high-efficiency production of 2-keto-Lgulonic acid. The results showed that the consortium had a symbiotic relationship, with the helper strain promoting the growth of the producing strain and increasing the production of 2-KLG. Additionally, the consortium exhibited reduced oxidative stress.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhijie Qin, Yue Chen, Shiqin Yu, Jian Chen, Jingwen Zhou
Summary: The potential SNDH/SDH gene cluster in Gluconobacter cerinus CGMCC 1.110 strain was identified and its function in transforming L-sorbose to 2-KLG was confirmed. Fermentation results showed that SDH was the limiting enzyme. 2-KLG titer was increased to 51.9 g/L through a metabolic engineering process.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yue Chen, Li Liu, Shiqin Yu, Jianghua Li, Jingwen Zhou, Jian Chen
Summary: The acetic acid bacterium Gluconobacter oxydans is widely used in industrial production due to its unique incomplete oxidation process, with 2-keto-L-gulonic acid (2-KLG) being one of the compounds produced. Promoters play a crucial role in controlling gene expression, and in this study, the low activity of SDH in G. oxydans was addressed by enhancing SDH expression using newly identified promoters. The study identified several strong promoters that significantly increased the yield of 2-KLG in G. oxydans, providing new tools for metabolic engineering in the production of high-value products.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Mingfu Gao, Hao Sun, Meijun Shi, Qiqi Wu, Dongxu Ji, Bing Wang, Lixin Zhang, Yang Liu, Litao Han, Xicheng Ruan, Hui Xu, Weichao Yang
Summary: The study demonstrated that the application of 2KGA can enhance crop resistance to salt stress, improve leaf and root biomass, increase levels of stress-related metabolites and enzymes, decrease hydrogen peroxide and malondialdehyde contents, and alleviate lipid peroxidation and cell membrane damage. Furthermore, positive correlations were found between various parameters such as photosynthetic pigments, organic solutes, ASA, and antioxidant enzymes, while negative correlations were observed between antioxidant enzymes and H2O2/MDA. The upregulation of certain genes involved in ASA metabolism suggested a critical mechanism underlying the promotion of plant growth under salt stress through exogenous 2KGA application.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Chemical
Lei Zhang, Hao Zhang, Huijian Shang
Summary: By studying the mass transfer rule and applying the reaction-membrane separation coupling technology to the esterification process of 2-Keto-L-gulonic acid and ethanol, the esterification rate was increased to 95.37%.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2021)
Article
Agricultural Engineering
Fan Li, Cai-Yun Wang, Ying-Cai Wu, Meng-Yue Zhang, Yi-Jin Wang, Xun-Yong Zhou, Yi-Xuan Zhang
Summary: This study presents a comprehensive metabolic engineering strategy to optimize the one-step fermentation process of 2-KGA. By genetic recombination and implementing four metabolic engineering strategies, a significant improvement in the production of 2-KGA was achieved, with a remarkable 15.48-fold increase.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Dong Li, Li Liu, Zhijie Qin, Shiqin Yu, Jingwen Zhou
Summary: This study successfully improved the ability of G. oxydans to directly produce 2-KLG from D-sorbitol through evolutionary and metabolic engineering.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Dong Li, Zhiwei Deng, Xiaodong Hou, Zhijie Qin, Xinglong Wang, Dejing Yin, Yue Chen, Yijian Rao, Jian Chen, Jingwen Zhou
Summary: By solving the crystal structures of SNDH from Gluconobacter oxydans WSH-004, a reversible disulfide bond between Cys295 and the catalytic Cys296 residues is discovered, allowing SNDH to switch between oxidation and reduction states. Moreover, Cys296 is found to affect the binding of NADP(+) with SNDH. The study proposes the redox-based dynamic regulation and catalytic mechanisms of SNDH through in vitro biochemical and site-directed mutagenesis studies.
Article
Agricultural Engineering
Dong Li, Xinglong Wang, Zhijie Qin, Shiqin Yu, Jian Chen, Jingwen Zhou
Summary: Mutants of L-sorbose dehydrogenase (SDH) were designed to enhance the production of 2-keto-L-gulonic acid (2-KLG). A combination of SDH engineering and fermentation optimization effectively increased the production of 2-KLG.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Meijun Shi, Mingfu Gao, Hao Sun, Weichao Yang, Hongxia Zhao, Lixin Zhang, Hui Xu
Summary: Supplementing with exogenous 2-keto-L-gulonic acid can enhance the accumulation of L-ascorbic acid in aquatic animals, improve growth performance and feed utilization, providing a novel strategy for elevating L-ascorbic acid levels.
Article
Biochemistry & Molecular Biology
Yue Chen, Dong Li, Xiaoyu Shan, Jingwen Zhou, Jian Chen
Summary: In this study, the sorbose dehydrogenase of Gluconobacter oxydans WSH-004 was identified as a sorbose oxidase (SOX). The study also found that H2O2 inhibited the activity of SOX and identified phenazine methosulfate (PMS) as the best electron acceptor for SOX. Furthermore, the enzymatic catalysis of SOX and SNDH was conducted in vitro to produce 2-KLG from L-sorbose.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Physical
Fan Li, Cai-Yun Wang, Meng-Yue Zhang, Yi-Xuan Zhang
Summary: This study investigated eight L-sorbose dehydrogenases in K. vulgare SPU B805 and K. robustum SPU_B003, revealing their crucial roles in 2-KGA synthesis and proposing a novel whole-cell recycling catalysis system to achieve high productivity.
MOLECULAR CATALYSIS
(2022)
Article
Agricultural Engineering
Guang Li, Dong Li, Weizhu Zenga, Zhijie Qina, Jian Chena, Jingwen Zhou
Summary: Gluconobacter oxydans ATCC9937, a chassis strain, naturally synthesizes 2-KLG from D-glucose, and by enhancing the intracellular and extracellular glucose metabolic flux, a high yield of 30.5 g/L 2-KLG with a conversion ratio of 39.0% is achieved, providing a new approach for economical large-scale fermentation of vitamin C.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qian Zhang, Shuxia Lyu
Summary: The second stage of the typical vitamin C mixed-fermentation process involves bioconversion of L-sorbose to 2-keto-L-gulonic acid (2-KLG) using a consortium of Ketogulonicigenium vulgare and Bacillus spp. The concentration of the helper strain in the co-fermentation system is closely related to K. vulgare cell growth and 2-KLG accumulation. The addition of 2-KLG has different effects on the physiological and biochemical properties of Bacillus pumilus and K. vulgare, including reducing cell viability, influencing gene expression, and inducing oxidative stress.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Yang Wang, Donglei Zhao, Wei Zhang, Songshan Wang, Yu Wu, Songxue Wang, Yongtan Yang, Baoyuan Guo
Summary: A highly efficient DON-degrading bacterial strain D3_3, belonging to Ketogulonicigenium vulgare, was isolated in this study. This isolate could efficiently degrade DON under various conditions, and 3-keto-DON was identified as the sole metabolite. Four genes encoding alcohol dehydrogenases were found responsible for the DON oxidation reaction. The discovery of this DON-degrading isolate and its enzymes opens up possibilities for developing DON-detoxifying agents for food and animal feed.
Review
Biotechnology & Applied Microbiology
Wei Song, Xiulai Chen, Jing Wu, Jianzhong Xu, Weiguo Zhang, Jia Liu, Jian Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Rui Yao, Pei Zhou, Chengjin Wu, Liming Liu, Jing Wu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Jian-Zhong Xu, Hao-Zhe Ruan, Hai-Bo Yu, Li-Ming Liu, Weiguo Zhang
MICROBIAL CELL FACTORIES
(2020)
Article
Chemistry, Physical
Liang Guo, Wenwen Diao, Cong Gao, Guipeng Hu, Qiang Ding, Chao Ye, Xiulai Chen, Jia Liu, Liming Liu
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Wei Song, Jia Liu, Qiuling Luo, Liming Liu
Summary: Chassis engineering was used to enhance enzymatic production of Hyp in Escherichia coli, leading to significant improvements in Hyp titre, conversion rate and productivity. Rewiring the TCA cycle and l-proline degradation pathway increased alpha-ketoglutarate supply, while fine-tuning heterologous haemoglobin expression improved oxygen transfer, resulting in a more efficient method for industrial production of Hyp.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Cong Gao, Liang Guo, Qiang Ding, Guipeng Hu, Chao Ye, Jia Liu, Xiulai Chen, Liming Liu
METABOLIC ENGINEERING
(2020)
Article
Chemistry, Physical
Wei Song, Xin Xu, Cong Gao, Yuxuan Zhang, Jing Wu, Jia Liu, Xiulai Chen, Qiuling Luo, Liming Liu
Article
Biotechnology & Applied Microbiology
Xiulai Chen, Juyang Yi, Jia Liu, Qiuling Luo, Liming Liu
Summary: This study presents a green and efficient production process for Hyp by overexpressing DsP4H enzyme and optimizing culture parameters, achieving high yields and conversion rates.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Pei Zhou, Xiaoke Yuan, Hui Liu, Yanli Qi, Xiulai Chen, Liming Liu
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Xu Tan, Wei Song, Xiulai Chen, Liming Liu, Jing Wu
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Qiang Ding, Wenwen Diao, Cong Gao, Xiulai Chen, Liming Liu
BIOTECHNOLOGY ADVANCES
(2020)
Article
Biotechnology & Applied Microbiology
Jianshen Hou, Cong Gao, Liang Guo, Jens Nielsen, Qiang Ding, Wenxiu Tang, Guipeng Hu, Xiulai Chen, Liming Liu
METABOLIC ENGINEERING
(2020)
Review
Biochemical Research Methods
Liang Guo, Zixuan Pang, Cong Gao, Xiulai Chen, Liming Liu
CURRENT OPINION IN BIOTECHNOLOGY
(2020)
Review
Biotechnology & Applied Microbiology
Hui Liu, Yanli Qi, Pei Zhou, Chao Ye, Cong Gao, Xiulai Chen, Liming Liu
Summary: This review proposes a strategy of microbial physiological engineering (MPE) to improve the efficiency of microbial cell factories, focusing on enhancing substrate utilization, growth performance, stress tolerance, and product export capacity.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yang Li, Shujie Yang, Danlei Ma, Wei Song, Cong Gao, Liming Liu, Xiulai Chen
Summary: Organic acids, as building block compounds, are widely used in various industries with chemical synthesis being the primary method. Microbial cell factories offer a promising approach for sustainable organic acid production but face challenges in cellular metabolism regulation. Metabolic engineering strategies have been developed to reprogram microbial cell factories for higher productivity of organic acids by improving enzyme efficiency, balancing biosynthetic pathways, enhancing metabolic flux, optimizing the metabolic network, and broadening substrate utilization.
NATURAL PRODUCT REPORTS
(2021)