期刊
JOURNAL OF BIOTECHNOLOGY
卷 165, 期 3-4, 页码 184-194出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.04.003
关键词
Metabolic engineering; Yarrowia lipolytica; Pentane; Alkanes
资金
- Office of Naval Research Young Investigator Program Award
- Welch Foundation [F-1753]
The complete biosynthetic replacement of petroleum transportation fuels requires a metabolic pathway capable of producing short chain n-alkanes. Here, we report and characterize a proof-of-concept pathway that enables microbial production of the C-5 n-alkane, pentane. This pathway utilizes a soybean lipoxygenase enzyme to cleave linoleic acid to pentane and a tridecadienoic acid byproduct. Initial expression of the soybean lipoxygenase enzyme within a Yarrowia lipolytica host yielded 1.56 mg/L pentane. Efforts to improve pentane yield by increasing substrate availability and strongly overexpressing the lipoxygenase enzyme successfully increased pentane production three-fold to 4.98 mg/L. This work represents the first-ever microbial production of pentane and demonstrates that short chain n-alkane synthesis is conceivable in model cellular hosts. In this regard, we demonstrate the potential pliability of Y. lipolytica toward the biosynthetic production of value-added molecules from its generous fatty acid reserves. (C) 2013 Elsevier B.V. All rights reserved.
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