期刊
JOURNAL OF BIOTECHNOLOGY
卷 168, 期 1, 页码 101-106出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2013.07.033
关键词
Escherichia coli; Gluconic acid; Ethanol; Pathway
资金
- Agriculture and Food Research Initiative Competitive Grant [011-67009-20060]
- USDA National Institute of Food and Agriculture and California Energy Commission [55779A/08-03]
- Cota-Robles
- EPA STAR fellowships
We report on engineering Escherichia coli to produce ethanol at high yield from gluconic acid (gluconate). Knocking out genes encoding for the competing pathways (L-lactate dehydrogena se and pyruvate formate lyase A) in E. coli 1(011 eliminated lactate production, lowered the carbon flow toward acetate production, and improved the ethanol yield from 87.5% to 97.5% of the theoretical maximum, while the growth rate of the mutant strain was about 70% of the wild type. The corresponding genetic modifications led to a small improvement of ethanol yield from 101.5% to 106.0% on glucose. Deletion of the pyruvate dehydrogenase gene (pdh) alone improved the ethanol yield from 87.5% to 90.4% when gluconate was a substrate. The growth rate of the mutant strain was identical to that of the wild type. The corresponding genetic modification led to no improvements on ethanol yield on glucose. (C) 2013 Elsevier B.V. All rights reserved.
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