Review
Immunology
Karolina D. Witt
Summary: MHC class I antigen processing is a less recognized area in nonviral host-pathogen interactions, involving immunology and cell biology. This review focuses on the MHC-I antigen processing pathway and alternative sources of antigens, particularly in the context of Mycobacterium tuberculosis (Mtb) as an intracellular pathogen. It explores how Mtb manipulates host immunity for survival and proposes directions for MHC-I-focused approaches in developing vaccines against tuberculosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Immunology
Karthik Dhatchinamoorthy, Jeff D. Colbert, Kenneth L. Rock
Summary: Loss of MHC I antigen presentation is common in many cancers, which may impair immune responses and affect the efficacy of immunotherapy. Studies have discussed underlying mechanisms through which some cancers evade immune killing by shutting down the MHC I pathway, and proposed potential strategies to overcome this limitation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijing Wei, Zhenhai Tang, Guo Chen, Bing Wei, Yunxia Cao
Summary: In this study, the researchers found that upregulated autophagy and decreased expression of MHC-I and NLRC5 were associated with endometrial cancer (EC). Inhibition of autophagy was found to suppress MHC-I gene expression. They also discovered that LC3 interacted with NLRC5 to inhibit the NLRC5-mediated MHC-I antigen presentation pathway. These findings suggest that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy for EC management.
Article
Microbiology
Jun Li, Rumeng Li, Qi Zhang, Peipei Peng, Xiaoquan Wang, Min Gu, Zenglei Hu, Xinan Jiao, Daxin Peng, Jiao Hu, Xiufan Liu
Summary: This study demonstrates that the H7N9 VLP vaccine provides complete clinical protection against HP H7N9 virus, significantly inhibits virus shedding, and abolishes viral replication in chickens. The VLP vaccine represents a promising alternative strategy for the development of novel H7N9 avian influenza vaccines for chickens.
VETERINARY MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lauren E. Stopfer, Nicholas J. Rettko, Owen Leddy, Joshua M. Mesfin, Eric Brown, Shannon Winski, Bryan Bryson, James A. Wells, Forest M. White
Summary: Combining multiple therapeutic strategies may improve survival benefits for NRAS/BRAF mutant melanoma patients. This study utilized immunopeptidomics to measure the impact of MEK inhibition on anti-tumor immunity and identified enhanced expression of specific tumor antigens following therapy. Targeting these upregulated tumor antigens can enhance cell killing.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
David H. Margulies, Jiansheng Jiang, Javeed Ahmad, Lisa F. Boyd, Kannan Natarajan
Summary: Peptide loading of MHC-I molecules plays a critical role in the response of T cells to infections and tumors. Recent advances in structural methods have improved our understanding of the biophysical parameters that govern peptide selection, binding, and surface display. The current understanding of this cellular process is based on various approaches, and further studies are needed to explore its details and potential applications in immunization and therapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
M. L. M. Jongsma, J. Neefjes, R. M. Spaapen
Summary: MHC-I molecules present a blueprint of the intracellular proteome to T cells for immune response, but pathogens and tumor cells can downmodulate MHC-I mediated antigen presentation to evade immune surveillance. While the fundamental rules of antigen presentation are well understood, new modules of regulation in this system continue to be uncovered.
MOLECULAR IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jieru Deng, Chunni Lu, Chuanxin Liu, Sara Oveissi, W. Douglas Fairlie, Erinna F. Lee, Pamuk Bilsel, Hamsa Puthalakath, Weisan Chen
Summary: The study demonstrates that IAV infection induces endogenous presentation of a viral epitope by MHC-II to CD4(+) T cells, dependent on de novo protein synthesis and the ER-Golgi network. Some antigenic peptides are of cytosolic origin in this process. Additionally, autophagy inhibitors and deletion of autophagy-related genes block most of the MHC-II-restricted endogenous IAV antigen presentation.
Article
Microbiology
Jiao Hu, Qi Zhang, Peipei Peng, Rumeng Li, Jun Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Mei Mei, Xinan Jiao, Daxin Peng, Xiufan Liu
Summary: This study generated a H7N9 virus-like particle (VLP) vaccine and evaluated its efficacy in chickens and mice. The H7N9 VLP induced robust antibody immune responses and provided complete clinical protection against H7N9 virus challenge in both animal models. Additionally, the vaccine significantly reduced virus shedding and alleviated pulmonary lesions. Moreover, the H7N9 VLP also induced high T cell immunity and decreased the expression of inflammatory cytokines in mouse lungs. These findings highlight the potential of VLP-based vaccines as an alternative to traditional egg-based vaccines for controlling H7N9 influenza virus in both humans and poultry.
VETERINARY MICROBIOLOGY
(2022)
Review
Immunology
Lucas J. Kerstetter, Stephen Buckley, Carly M. Bliss, Lynda Coughlan
Summary: The ongoing threat to global health posed by infectious diseases, which have the potential for spillover from animal reservoirs, has increased in frequency due to changes in human behavior. Influenza A viruses present a pandemic threat and are a major concern for human health, with challenges in vaccine development. Non-replicating adenoviral vectors offer advantages for vaccine development against highly pathogenic avian influenza viruses, with exemplary safety and immunogenicity profiles.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Jiao Hu, Peipei Peng, Jun Li, Qi Zhang, Rumeng Li, Xiaoquan Wang, Min Gu, Zenglei Hu, Shunlin Hu, Xiaowen Liu, Xinan Jiao, Daxin Peng, Xiufan Liu
Summary: The bivalent H5+H7 VLP vaccine candidate shows promising results in protecting poultry against highly pathogenic H5N1 and H7N9 avian influenza viruses, and may serve as a critical alternative to traditional egg-based inactivated vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Jeff D. Colbert, Freidrich M. Cruz, Christina E. Baer, Kenneth L. Rock
Summary: This study reveals a molecular association between Tspan5 and MHC I molecules that is critical for the formation of MHC I nanoclusters and optimal T cell responses to antigens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Maria C. Tovar Fernandez, Ewa M. Sroka, Mathilde Lavigne, Aikaterini Thermou, Chrysoula Daskalogianni, Benedicte Manoury, Rodrigo Prado Martins, Robin Fahraeus
Summary: The accumulation of protein aggregates is toxic and its relation to diseases is still unclear. This study reveals that certain antigenic peptides can be presented to the MHC class I pathway via autophagy. The presentation of peptides derived from the viral EBNA1 protein was not affected by autophagy suppression, while the presentation of ovalbumin was suppressed. The study also suggests that the relative levels of protein expression do not affect autophagy-mediated antigen presentation.
CELLULAR IMMUNOLOGY
(2022)
Article
Immunology
Tobias Held, Michael Basler, Klaus-Peter Knobeloch, Marcus Groettrup
Summary: ISG15, induced by type I IFNs, may enhance MHC-I restricted antigen presentation by promoting co-translational antigen processing, without directly inducing proteasomal degradation of ISG15 target proteins.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Editorial Material
Immunology
Antonius A. de Waard, Tamara Verkerk, Marlieke L. M. Jongsma, Kelly Hoefakker, Sunesh Sethumadhavan, Carolin Gerke, Sophie Bliss, Xiangrui Kong, George M. C. Janssen, Arnoud H. de Ru, Frans H. J. Claas, Arend Mulder, Robert Tampe, Peter A. van Veelen, Anne Halenius, Robbert M. Spaapen
Summary: PAKC is a novel panel of ten cell lines that have individual components of the HLA class I antigen presentation pathway knocked out, providing a single model system for studies across multiple areas. This system is expected to accelerate research in oncology, infectiology, and autoimmunity.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Infectious Diseases
Ching-Ping Tsai, Hsiang-Jung Tsai
INFLUENZA AND OTHER RESPIRATORY VIRUSES
(2019)
Article
Biochemical Research Methods
S. Y. Yang, W. C. Wang, C. B. Lan, C. H. Chen, J. J. Chieh, H. E. Horng, Chin-Yih Hong, H. C. Yang, C. P. Tsai, C. Y. Yang, I. C. Cheng, W. C. Chung
JOURNAL OF VIROLOGICAL METHODS
(2010)
Article
Immunology
Chi-Yuan Chen, Hung-Jen Liu, Ching-Ping Tsai, Cheng-Yu Chung, Yung-Shen Shih, Poa-Chun Chang, Yung-Tsung Chiu, Yu-Chen Hu
Letter
Veterinary Sciences
CP Tsai, MJ Pan
Article
Microbiology
CP Tsai, CH Pan, MY Liu, YL Lin, CM Chen, TS Huang, IC Cheng, MH Jong, PC Yang
VETERINARY MICROBIOLOGY
(2000)