Article
Microbiology
Yong Qi, Shan-Shan Wang, Lu-Lin Li
Summary: Late gene expression of baculovirus depends on the virus-encoded RNA polymerase. The immediate-early gene ie1 plays essential roles in viral early gene expression, late gene expression, and DNA replication. IE1 can independently activate late gene expression, showing nonspecific transcription start sites.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Jianan Huang, Huanlei Liu, Xiaodong Xu
Summary: The baculovirus expression vector system (BEVS) is widely used for producing recombinant proteins. However, there is a risk of homologous recombination between viral genome and functional baculovirus-derived elements associated with foreign genes, which can lead to defective viruses and decreased recombinant protein yields. Shortening the length of homologous fragments and separating them by point mutations can effectively reduce the unfavorable recombination.
Article
Microbiology
Leonardo Assis da Silva, Monique da Rocha Queiroz Lima, Brenda Rabello de Camargo, Dyeferson Kened da Silva Coelho Guimaraes, Anabele Azevedo Lima Barbastefano, Raquel Curtinhas de Lima, Paulo Vieira Damasco, Rivaldo Venancio da Cunha, Luiz Jose de Souza, Elzinandes Leal de Azeredo, Luzia Maria De-Oliveira-Pinto, Tatsuya Nagata, Daniel M. P. Ardisson-Araujo, Flavia Barreto dos Santos, Bergmann Morais Ribeiro
Summary: CHIKV is a globally distributed arbovirus, and its laboratory diagnosis is mainly performed by molecular and serological assays. This research developed an ELISA test based on a multi-epitope recombinant protein for specific detection of anti-CHIKV antibodies, showing high sensitivity and specificity with no cross-reactivity.
Article
Engineering, Chemical
Carina Bannach, Daniel Ruiz Buck, Genna Bobby, Leo P. Graves, Sainan Li, Adam C. Chambers, Elizabeth Gan, Raquel Arinto-Garcia, Robert D. Possee, Linda A. King
Summary: Autographa californica nucleopolyhedrovirus is an efficient expression vector for recombinant proteins in insect cells. The study found that using the AUA codon may slightly improve protein yield, while the AUU codon may lead to leaky translation.
Article
Entomology
Carol P. Wu, Chia-Jung Chang, Cheng-Hsun Li, Yueh-Lung Wu
Summary: The study found that the expression of the E2 gene carried by the recombinant baculovirus was stable for up to 10 passages, without affecting the production of recombinant E2 protein.
ENTOMOLOGICAL RESEARCH
(2021)
Article
Virology
Jeffrey J. Hodgson, Nicolas Buchon, Gary W. Blissard
Summary: Little is known about the trafficking process of viral envelope proteins in insect cells. In this study, a stable Drosophila cell line was engineered to investigate the host proteins and cellular trafficking pathways involved in the delivery of the baculovirus envelope protein GP64 to the plasma membrane. Through a targeted RNA interference screen, several host genes were identified to be important for the trafficking of GP64 to the cell surface. The findings suggest that recycling plays a role in maintaining GP64 at the cell surface. This study provides insight into the understanding of how insect virus envelope proteins are transported and contributes to the knowledge of virus infection processes.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemical Research Methods
Chandra Kanth Bandi, Kyle S. Skalenko, Ayushi Agrawal, Neelan Sivaneri, Margaux Thiry, Shishir P. S. Chundawat
Summary: The study developed an engineered synthetic promoter system called cyn regulon to selectively detect azide ions and induce downstream expression of reporter genes. The engineered cyn azide operon showed highly tunable expression over a range of azide ion concentrations and rapidly induced reporter gene expression.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Beata Flachner, Krisztina Dobi, Anett Benedek, Sandor Cseh, Zsolt Lorincz, Istvan Hajdu
Summary: This paper describes the expression of RI in baculovirus-infected High Five cells and compares the differences between RI expressed in insect cells and commercially available products. The results show that RI expressed in insect cells has higher thermal stability and is endotoxin-free, making it potentially useful for future therapeutics.
Article
Immunology
Manon M. J. Cox
Summary: The insect cell expression system, proposed as the preferred biosecurity strategy for vaccine production, has limitations that need to be addressed for effective use in future pandemics.
Article
Microbiology
He Yan, Jun Feng, Min Chen
Summary: This study conducted structural prediction analysis on two proteins, Pfk13-F446I and Pfk13-0580Y, which are closely associated with artemisinin resistance. The predicted structures revealed that Pfk13-F446I is located near the central protein cavity, while Pfk13-0580Y is located on the central channel. These mutations may lead to conformational changes in the protein structure.
Article
Biochemistry & Molecular Biology
Morikazu Imamura, Naoko Tabeta, Yoshifumi Iwamaru, Hanae Takatsuki, Tsuyoshi Mori, Ryuichiro Atarashi
Summary: This study successfully generated various Bac-rPrP(Sc) variants with different transmissibility and structural properties using a baculovirus-insect cell expression system, and found that they compete with each other and gradually converge in ultrasonic reactions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Krishna Kumar Haridhasapavalan, Pradeep Kumar Sundaravadivelu, Neha Joshi, Nayan Jyoti Das, Anshuman Mohapatra, Udayashree Voorkara, Vishwas Kaveeshwar, Rajkumar P. Thummer
Summary: This study reports the generation of recombinant human HAND2 fusion protein using a heterologous system. The purified protein retained its secondary structure and could transduce cells and translocate to the nucleus. Additionally, the recombinant protein showed angiogenic potential and synergistically activated the α-MHC promoter.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Gema Lozano Terol, Julia Gallego-Jara, Rosa Alba Sola Martinez, Adrian Martinez Vivancos, Manuel Canovas Diaz, Teresa de Diego Puente
Summary: The research evaluated the expression of YFP reporter protein under the control of different strength promoters and replication origins, with different E. coli strains and carbon sources. It was found that metabolic burden leads to decreased expression of recombinant proteins, highlighting the importance of balancing plasmid copy number and promoter strength for optimal expression. The results represent a significant advance in determining the most suitable expression system for improving both the quantity and quality of recombinant proteins in bioproduction engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemical Research Methods
Carissa Grose, Zoe Putman, Dominic Esposito
Summary: Despite the success of generating recombinant proteins in insect cells using the Baculovirus Expression Vector System, challenges still exist, such as cytopathic effects, unpredictable co-infection and co-expression, and baculovirus genome instability. A better understanding of viral gene regulation and different promoter options is needed to advance the platform for expressing a greater variety of proteins and complexes in insect cells.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Multidisciplinary Sciences
Gabor Torma, Dora Tombacz, Norbert Moldovan, Adam Fulop, Istvan Prazsak, Zsolt Csabai, Michael Snyder, Zsolt Boldogkoi
Summary: In this research, the transcriptional characterization of Autographa californica multiple nucleopolyhedrovirus was conducted using two long-read sequencing techniques, MinION and Sequel. A total of 875 transcript species were detected, including 759 novel ones. Additionally, novel replication origin-associated transcripts and upstream open reading frames were identified.
SCIENTIFIC REPORTS
(2022)
Article
Entomology
Ismael Sanchez-Ramos, Cristina E. Fernandez, Manuel Gonzalez-Nunez
JOURNAL OF PEST SCIENCE
(2019)
Article
Entomology
Ismael Sanchez-Ramos, Eduardo Gomez-Casado, Cristina E. Fernandez, Manuel Gonzalez-Nunez
ENTOMOLOGIA GENERALIS
(2019)
Article
Entomology
Esther Lantero, Marta Ortega, Ismael Sanchez-Ramos, Manuel Gonzalez-Nunez, Cristina E. Fernandez, Alejandro J. Rescia, Beatriz Matallanas, Carmen Callejas, Susana Pascual
Article
Entomology
Celeste Azpiazu, Pilar Medina, Angeles Adan, Ismael Sanchez-Ramos, Pedro del Estal, Alberto Fereres, Elisa Vinuela
Article
Agronomy
Inigo Loureiro, Ines Santin-Montanya, Maria-Concepcion Escorial, Esteban Garcia-Ruiz, Guillermo Cobos, Ismael Sanchez-Ramos, Susana Pascual, Manuel Gonzalez-Nunez, Maria-Cristina Chueca
Article
Entomology
Esteban Garcia-Ruiz, Guillermo Cobos, Ismael Sanchez-Ramos, Susana Pascual, Maria-Cristina Chueca, Maria-Concepcion Escorial, Ines Santin-Montanya, Inigo Loureiro, Manuel Gonzalez-Nunez
Summary: The combination of genetically modified maize varieties tolerant to herbicides and resistant to insects, along with the application of glyphosate, may effectively control weeds and pests. However, the impact on nontarget arthropods still needs evaluation. Studying the population changes of canopy-dwelling arthropods in Bt maize under different management options, including glyphosate application, found no significant effects except for the decrease of certain groups in plots with no pre-emergence herbicides, particularly in treatments involving two glyphosate applications.
Article
Entomology
Manuel Gonzalez-Nunez, Susana Pascual, Ana Cobo, Elena Seris, Guillermo Cobos, Cristina E. Fernandez, Ismael Sanchez-Ramos
Summary: The study on controlling the olive fruit fly found that kaolin sprays effectively reduced damage to olives and ensured high-quality oil production. Copper treatments provided some protection but were insufficient at high infestation levels. However, the impact on non-target arthropods was relatively minor.
ENTOMOLOGIA GENERALIS
(2021)
Article
Entomology
Esteban Garcia-Ruiz, Susana Pascual, Manuel Gonzalez-Nunez, Guillermo Cobos, Inigo Loureiro, Ines Santin-Montanya, Maria-Concepcion Escorial, Maria-Cristina Chueca, Ismael Sanchez-Ramos
Summary: Field trials conducted in Central Spain showed that different management strategies for weed and pest control did not have a noticeable impact on the communities of ground-dwelling phytophagous and predatory arthropods, although minor changes were observed in the abundances of some taxa. The use of reduced doses of conventionally used herbicides resulted in increased populations of dominant ground beetle species. The stability of the ground-dwelling arthropod community was not significantly affected by any of the maize varieties or herbicide options assessed in this study.
ENTOMOLOGIA GENERALIS
(2022)
Letter
Infectious Diseases
Maria Pilar Romero-Gomez, Silvia Gomez-Sebastian, Emilio Cendejas-Bueno, Maria Dolores Montero-Vega
JOURNAL OF INFECTION
(2021)
Article
Immunology
Kevin P. Dalton, Carmen Alvarado, Edel Reytor, Maria Del Carmen Nunez, Ana Podadera, Diego Martinez-Alonso, Jose Manuel Martin Alonso, Ines Nicieza, Silvia Gomez-Sebastian, Romy M. Dalton, Francisco Parra, Jose M. Escribano
Summary: The VP60 capsid protein from rabbit haemorrhagic disease virus can form virus-like particles when expressed using different protein expression systems. In this study, a dual-serving RHDV vaccine was successfully developed using CrisBio technology, which allows the co-expression of VP60 proteins from two RHDV serotypes. Rabbits immunised with the chimeric VLPs were fully protected against a lethal challenge infection with the two RHDV serotypes, demonstrating the effectiveness of the dual vaccine strategy.
Article
Agronomy
Manuel Gonzalez-Nunez, Pilar Sandin-Espana, Miguelina Mateos-Miranda, Guillermo Cobos, Antonieta De Cal, Ismael Sanchez-Ramos, Jose-Luis Alonso-Prados, Inmaculada Larena
Summary: A new integrated pest management (New IPM) protocol has been implemented in Spain for managing the main diseases and pests of sweet cherry. This protocol includes nonchemical strategies, adjusts the timing and number of pesticide applications based on damage thresholds and a predictive model, and reduces the use of pesticides significantly without affecting the control of diseases and pests.
Article
Biotechnology & Applied Microbiology
Ismael Sanchez-Ramos, Manuel Gonzalez-Nunez
Summary: The olive fruit fly is a major pest in crop production, and the use of environmentally safer biological control methods is needed. The study found that the parasitization rates and biological parameters of the Muscidifurax raptorellus parasitoid were favorable for controlling the olive fruit fly.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2023)
Article
Horticulture
Manuel Gonzalez-Nunez, Guillermo Cobos, Ismael Sanchez-Ramos
Summary: Rhagoletis cerasi (L.) is a damaging pest of cherries in Europe, Western Asia, and North America. The management of this pest mainly relies on insecticide applications near harvest, but the repeated use of insecticides poses environmental risks and may lead to resistance and fruit residues. This study compared trap-attractant combinations and found that a folded yellow sticky trap with a homemade ammonium acetate dispenser was the most efficient and cost-effective device for mass trapping of R. cerasi. Farm-scale trials showed that mass trapping can reduce the need for insecticide applications and keep damage below the economic threshold, making it a useful tool for managing R. cerasi.
Article
Microbiology
Carlos Garcia-Benitez, Carla Casals, Josep Usall, Ismael Sanchez-Ramos, Paloma Melgarejo, Antonieta De Cal
Article
Entomology
Marta Ortega, Ismael Sanchez-Ramos, Manuel Gonzalez-Nunez, Susana Pascual
AGRICULTURAL AND FOREST ENTOMOLOGY
(2018)