Article
Microbiology
Manish Kumar, Supreet Saini, Kalyan Gayen
Summary: We developed a pH-based phenomenological model to predict the fermentative production of butanol from glucose using C. acetobutylicum in a batch system. The model describes the relationship between the dynamics of growth and the production of desired metabolites and the extracellular pH of the media. Our model was successful in predicting the fermentation dynamics of C. acetobutylicum and can be extended to represent the dynamics of butanol production in other fermentation systems.
Article
Multidisciplinary Sciences
Celine Foulquier, Antoine Riviere, Mathieu Heulot, Suzanna Dos Reis, Caroline Perdu, Laurence Girbal, Mailys Pinault, Simon Dusseaux, Minyeong Yoo, Philippe Soucaille, Isabelle Meynial-Salles
Summary: The authors identified the previously unknown encoding genes for important enzymes involved in redox balancing in n-butanol production by Clostridium acetobutylicum, and increased n-butanol production through enzyme optimization. This discovery is significant for the development of a homobutanologenic C. acetobutylicum strain.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Guangqing Du, Chao Zhu, Mengmeng Xu, Lan Wang, Shang-Tian Yang, Chuang Xue
Summary: This study identified four orphan histidine kinases (HKs) that control sporulation and solventogenesis in Clostridium acetobutylicum. Single knockout of cac3319 halted sporulation at the initiation stage but increased butanol tolerance and production, while cac0437 knockout caused premature sporulation and inhibited the transition to solventogenesis, resulting in low butanol production.
Article
Biochemistry & Molecular Biology
Guangqing Du, Youduo Wu, Wei Kang, Yongping Xu, Shuying Li, Chuang Xue
Summary: In this study, the strain Clostridium acetobutylicum ATCC 55025 and its mutant HKKO were engineered to improve butanol production. Knocking out the acetoacetate decarboxylase gene adc decreased acetone production and increased the butanol/ABE ratio. Overexpression of the acetaldehyde/alcohol dehydrogenase gene adhE2 further increased butanol production.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Zongjie Dai, Yan Zhu, Hongjun Dong, Chunhua Zhao, Yanping Zhang, Yin Li
Summary: Depletion of intracellular ATP significantly increased glycolytic rate, enhanced solvent production, and resulted in a wide range of metabolic changes in C. acetobutylicum DSM 1731. This finding provides a novel strategy for engineering solvent-producing C. acetobutylicum and other anaerobic microbial cell factories.
MICROBIAL CELL FACTORIES
(2021)
Review
Biotechnology & Applied Microbiology
Petra Patakova, Barbora Branska, Maryna Vasylkivska, Katerina Jureckova, Jana Musilova, Ivo Provaznik, Karel Sedlar
Summary: Solventogenic clostridia, although not a strictly defined group within the genus Clostridium, share common features such as being anaerobic, non-pathogenic, non-toxinogenic, and endospore forming bacteria. Their main metabolite is typically 1-butanol, but they can also produce other metabolites depending on species and culture conditions. Despite being used for industrial production in the past, they were replaced by more efficient chemical production methods. Transcriptome analysis is a valuable tool for gaining deeper insights into clostridial metabolism and life.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Guangqing Du, Jian Che, Youduo Wu, Zhenzhong Wang, Zhiyang Jiang, Feng Ji, Chuang Xue
Summary: In this study, the disruption of hydA gene in Clostridium acetobutylicum led to increased butanol production and decreased acetone formation. Exogenous supplementation of methyl viologen further improved the butanol yield and ratio. The engineered strain showed potential for producing biobutanol using lignocellulosic biomass.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Wei-Lun Chang, Wenjie Hou, Mengmeng Xu, Shang-Tian Yang
Summary: This study achieved continuous production of butanol from glucose and butyrate by immobilizing cells in a single-pass fibrous-bed bioreactor. The high density of immobilized cells improved the tolerance to butanol and butyric acid, resulting in significantly increased productivity.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Engineering, Environmental
Marwa M. El-Dalatony, Bikram Basak, Mayur B. Kurade, Hyun-Seog Roh, Min Jang, Byong-Hun Jeon
Summary: This study demonstrates the effective production of biohydrogen (bioH(2)) and ABE through ABE fermentation using sonication pretreated Chlamydomonas mexicana biomass as a cost-effective substrate. Sonication enhances the recovery and bioaccessibility of carbohydrates and proteins, resulting in higher yields of bioH(2) and volatile fatty acids. The study shows the efficient conversion of soluble carbohydrates and proteins into bioH(2) and ABE, which can be used for high-energy biofuel production with minimal waste generation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Energy & Fuels
F. Raganati, A. Procentese, G. Olivieri, M. E. Russo, P. Salatino, A. Marzocchella
Summary: This study demonstrates the intensive production of butanol using immobilized cells of Clostridium acetobutylicum in a novel continuous fermentation system. By implementing a stagewise simulated moving bed design and integrating the reaction and the product recovery sections, the system achieves high productivity and concentration of butanol.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Agricultural Engineering
Ruiling Gao, Lian Xiong, Mengkun Wang, Fen Peng, Hairong Zhang, Xinde Chen
Summary: Coupled fermentation of Clostridium acetobutylicum and oleaginous yeasts was conducted to produce acetone-butanol-ethanol (ABE) and lipids from sugarcane molasses. The results showed that the ABE fermentation wastewaters could be effectively treated and the added value of the products could be increased through coupling with lipid-producing fermentation process. Different types of wastewaters were analyzed and classified based on their carbon source content and structure. Oleaginous yeasts, including Trichosporon dermatis and Lipomyces starkeyi, were cultured on the two types of wastewaters and their lipid productions and removals of COD and ammonia were evaluated. The findings suggest that Trichosporon dermatis performed well on both types of wastewaters, while Lipomyces starkeyi achieved the highest lipid production on Type-2 wastewater.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Physical
Ye-Seung Son, Jong-Min Jeon, Do-Hyung Kim, Yung-Hun Yang, Yong-Su Jin, Byung-Kwan Cho, Sang-Hyoun Kim, Sunil Kumar, Byung-Don Lee, Jeong-Jun Yoon
Summary: This study successfully improved hydrogen productivity in Clostridium acetobutylicum through metabolic engineering by introducing zwf and hydA genes, leading to a significant increase in hydrogen yield under optimized pH and glucose concentration conditions. These findings provide insights for future directions in metabolic engineering for enhanced hydrogen production in C. acetobutylicum.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Energy & Fuels
Zhiyou Zhou, Yujie Jing, Saijin Wei, Qinghua Zhang, Shuaiying Peng, Xuejiao An, Hanguang Li
Summary: In this study, an exogenous regulatory strategy named oxidation-reduction potential (ORP) regulation was used to enhance butanol production. The findings showed that controlling the ORP value at -400 mV resulted in the highest butanol production, with increased yield and productivity. The study also investigated the physiological mechanisms of ORP regulation on butanol synthesis.
Article
Biotechnology & Applied Microbiology
Karlo Guerrero, Roberto Gallardo, Ernesto Gonzalez, Fabian Veliz, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: By adding butyrate and neutral red to the culture medium, and using electro-fermentation with an external electron source, the production of butanol can be increased. However, the presence of butyrate and the absence of neutral red in electro-fermentation are detrimental to the microorganism.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Youduo Wu, Zhenzhong Wang, Xiao Ma, Chuang Xue
Summary: By optimizing high-temperature simultaneous saccharification and fermentation (SSF) technology and using genetically engineered thermotolerant Clostridium acetobutylicum, the production and productivity of lignocellulosic butanol were successfully increased, demonstrating its potential in improving butanol production efficiency.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Juergen Behr, Andreas J. Geissler, Patrick Preissler, Armin Ehrenreich, Angel Angelov, Rudi F. Vogel
Article
Microbiology
David Kostner, Michael Rachinger, Wolfgang Liebl, Arinin Ehrenreich
Article
Biotechnology & Applied Microbiology
Bjoern Peters, Anja Junker, Katharina Brauer, Bernadette Muehlthaler, David Kostner, Markus Mientus, Wolfgang Liebl, Armin Ehrenreich
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2013)
Review
Biotechnology & Applied Microbiology
Wolfgang Liebl, Angel Angelov, Julia Juergensen, Jennifer Chow, Anita Loeschcke, Thomas Drepper, Thomas Classen, Joerg Pietruzska, Armin Ehrenreich, Wolfgang R. Streit, Karl-Erich Jaeger
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
D. Kostner, B. Luchterhand, A. Junker, S. Volland, R. Daniel, J. Buechs, W. Liebl, A. Ehrenreich
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Daniel Hoenicke, Tina Luetke-Eversloh, Ziyong Liu, Doerte Lehmann, Wolfgang Liebl, Armin Ehrenreich
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Ching-Ning Huang, Wolfgang Liebl, Armin Ehrenreich
BIOTECHNOLOGY FOR BIOFUELS
(2018)
Article
Microbiology
Daniela Kaspar, Franziska Auer, Jakob Schardt, Franziska Schindele, Alberto Ospina, Claudia Held, Armin Ehrenreich, Siegfried Scherer, Stefanie Mueller-Herbst
FEMS MICROBIOLOGY LETTERS
(2014)
Article
Biotechnology & Applied Microbiology
Justyna Maria Lesiak, Wolfgang Liebl, Armin Ehrenreich
JOURNAL OF BIOTECHNOLOGY
(2014)
Article
Biochemical Research Methods
Yvonne Kohlmann, Anne Pohlmann, Edward Schwartz, Daniela Zuehlke, Andreas Otto, Dirk Albrecht, Christina Grimmler, Armin Ehrenreich, Birgit Voigt, Doerte Becher, Michael Hecker, Baerbel Friedrich, Rainer Cramm
JOURNAL OF PROTEOME RESEARCH
(2014)
Article
Microbiology
Stefanie Mueller-Herbst, Stefanie Wuestner, Anna Muehlig, Daniela Eder, Thilo M. Fuchs, Claudia Held, Armin Ehrenreich, Siegfried Scherer
Article
Biochemical Research Methods
Nicole Rachfall, Kerstin Schmitt, Susanne Bandau, Nadine Smolinski, Armin Ehrenreich, Oliver Valerius, Gerhard H. Braus
MOLECULAR & CELLULAR PROTEOMICS
(2013)
Article
Multidisciplinary Sciences
Hedwig Kurka, Armin Ehrenreich, Wolfgang Ludwig, Marc Monot, Maja Rupnik, Frederic Barbut, Alexander Indra, Bruno Dupuy, Wolfgang Liebl
Article
Biotechnology & Applied Microbiology
Bjoern Peters, Markus Mientus, David Kostner, Rolf Daniel, Wolfgang Liebl, Armin Ehrenreich
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)
Article
Biotechnology & Applied Microbiology
Markus Mientus, David Kostner, Bjoern Peters, Wolfgang Liebl, Armin Ehrenreich
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2017)