Article
Chemistry, Multidisciplinary
Yujie Guo, Vivien Walter, Steven Vanuytsel, Christopher Parperis, Jason T. Sengel, Eve E. Weatherill, Mark I. Wallace
Summary: Methods combining spatially patterned activation of a photoreductant and interferometric scattering microscopy enable fast, label-free monitoring and control of hundreds of gold nanoparticles. The kinetics of individual particle growth follow a two-step nucleation-autocatalysis model with a distribution of rate constants that vary with reaction conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Multidisciplinary Sciences
Lydia Steffens, Eugenio Pettinato, Thomas M. Steiner, Achim Mall, Simone Koenig, Wolfgang Eisenreich, Ivan A. Berg
Summary: In Deltoproteobacterium Hippea maritima, the TCA cycle can be reversed by high CO2 partial pressures for autotrophic carbon fixation. This reversed oxidative TCA cycle could have functioned in autotrophic CO2 fixation in a primordial atmosphere rich in CO2.
Article
Cell Biology
Md Jawaid Akhtar, Shah Alam Khan, Bhupinder Kumar, Pooja Chawla, Rohit Bhatia, Karanvir Singh
Summary: This review discusses the structure, function, and regulation of the NaCT transporter, highlighting the role of citrate in diagnosing diseases such as cancer, diabetes, and fatty liver. The therapeutic potential of synthetic inhibitors against NaCT transporters is also summarized.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Marina Toplak, Raspudin Saleem-Batcha, Joern Piel, Robin Teufel
Summary: The article presents a spioketal synthase that is involved in the biosynthesis of stable chroman spiroketals, showcasing its multifunctional role in controlling the redox state of the substrate and cleavage of carbon-carbon bonds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Plant Sciences
Rodrigo Siqueira Reis, Yves Poirier
Summary: In plants, thousands of genes are associated with antisense transcription, producing noncoding RNAs that play diverse roles in gene regulation. The evidence suggests that antisense transcription is not primarily involved in gene silencing, but has a broad and diverse function in gene regulation. A framework for the functional dissection of antisense transcripts is proposed to move beyond the gene silencing paradigm.
TRENDS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Diaa Abd El-Moneim, Roberto Contreras, Javier Silva-Navas, Francisco Javier Gallego, Ana M. Figueiras, Cesar Benito
Summary: Aluminum toxicity in acid soils affects plant development and yield. Citrate plays a crucial role in plant defense against aluminum toxicity. This study identifies the mitochondrial citrate synthase gene as being repressed under aluminum stress in both rye and Brachypodium distachyon, with higher mRNA levels observed in aluminum-tolerant lines.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Miao Zhang, Feng-Quan Tan, Yan-Jie Fan, Ting-Ting Wang, Xin Song, Kai-Dong Xie, Xiao-Meng Wu, Fan Zhang, Xiu-Xin Deng, Jude W. Grosser, Wen-Wu Guo
Summary: Polyploidization leads to novel phenotypes, and the reprogramming of lysine acetylation plays an important role in trait evolution during this process, by regulating enzyme activity and protein abundance.
Article
Multidisciplinary Sciences
Kazuya Nishio, Tomoyuki Kawarasaki, Yuki Sugiura, Shunsuke Matsumoto, Ayano Konoshima, Yuki Takano, Mayuko Hayashi, Fumihiko Okumura, Takumi Kamura, Tsunehiro Mizushima, Kunio Nakatsukasa
Summary: Deficiencies in mitochondrial protein import are associated with diseases, but it is unclear how accumulation of non-imported mitochondrial proteins causes cell dysfunction. This study showed that non-imported citrate synthase is targeted for proteasomal degradation. Accumulation of non-imported citrate synthase caused ectopic citrate synthesis, leading to metabolic imbalance and growth defect. This study highlights the importance of mitochondrial import and its failure in cellular metabolism.
Article
Microbiology
Anil Pant, Lara Dsouza, Shuai Cao, Chen Peng, Zhilong Yang
Summary: Metabolism plays a crucial role in the interaction between host and viruses, with VACV inducing profound changes in cellular metabolism by increasing TCA intermediates and modulating cell signaling pathways. VACV-encoded viral growth factor stimulates elevation of citrate, promoting viral infection by activating non-canonical STAT3 signaling.
Article
Immunology
Miao Hu, Yajie Zhu, Yuqian Mo, Xu Gao, Meng Miao, Wei Yu
Summary: This study found that Bombyx mori nucleopolyhedrovirus (BmNPV) infection could up-regulate the expression and acetylation modification of Bombyx mori citrate synthase (BmCS). Acetylation of lysine 336 significantly reduced the enzyme activity, resulting in decreased cellular ATP production and mitochondrial membrane potential. The acetylation of lysine 336 and the reduction of BmCS expression weakened the proliferation of BmNPV and the generation of progeny viruses. These findings provide novel insights for the development of antiviral strategies.
MICROBIAL PATHOGENESIS
(2022)
Article
Agricultural Engineering
Yunxia Qin, Jianghua Yang, Zhiyong Wang, Yongjun Fang, Bin Hu, Xiaohu Xiao, Xiangyu Long
Summary: A mitochondrial citrate synthase (CS) gene named HbCS4 was isolated from the rubber tree and its role in energy supply was studied by comparing its expression patterns, enzyme activity, and metabolites assays under tapping and ethephon treatments.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Multidisciplinary Sciences
May N. Taw, Jason T. Boock, Belen Sotomayor, Daniel Kim, Mark A. Rocco, Dujduan Waraho-Zhmayev, Matthew P. DeLisa
Summary: The study suggests that the Tat translocase may use molecular chaperones to monitor the conformational status of its substrates, with the TatB component playing a key role in sensing the folding state of Tat substrates.
SCIENTIFIC REPORTS
(2022)
Article
Agronomy
Emanuele La Bella, Andrea Baglieri, Ermes Ivan Rovetto, Piergiorgio Stevanato, Ivana Puglisi
Summary: The study investigated the application of Chlorella vulgaris extract on lettuce seedlings, which positively influenced the growth of seedlings, increased chlorophylls, carotenoids, protein content, and ashes in shoots. Additionally, the extract also had a positive impact on the nitrogen metabolism in shoots and increased dry matter, proteins, and ash content in roots.
Article
Biochemistry & Molecular Biology
Maki Nishii, Shoki Ito, Takashi Osanai
Summary: This study investigated the biochemical properties of citrate synthase (CS) from C. merolae mitochondria (CmCS4). The results showed that CmCS4 exhibited higher catalytic efficiency for oxaloacetate and acetyl-CoA compared to cyanobacteria species. The high catalytic efficiency of CmCS4 may contribute to increased carbon flow into the tricarboxylic acid (TCA) cycle in C. merolae.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Alma Andersson, Joakim Lundeberg
Summary: In this study, a novel method was proposed to analyze spatial transcriptomics data, which simulated the diffusion of individual transcripts to extract genes with spatial patterns, generating satisfactory results on synthetic and real data. Compared to existing methods, this approach seemed to be less influenced by gene expression levels and showed better time performance when run with multiple cores.
Article
Chemistry, Multidisciplinary
Alexander K. Mengele, Dominik Weixler, Sebastian Amthor, Bernhard J. Eikmanns, Gerd M. Seibold, Sven Rau
Summary: This study successfully mimics the process of generating NADPH and ATP in the light-dependent reaction of photosynthesis by combining molecular ruthenium polypyridine-based photocatalysts and inverted vesicles derived from Escherichia coli.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Agnes Moe, Terezia Kovalova, Sylwia Krol, David J. Yanofsky, Michael Bott, Dan Sjostrand, John L. Rubinstein, Martin Hogbom, Peter Brzezinski
Summary: The study identified a respiratory supercomplex CIII2CIV2 in the Corynebacterium glutamicum strain, which catalyzes the oxidation of menaquinol and reduction of dioxygen, providing energy for the bacteria's redox processes. The structure of the C. glutamicum supercomplex was determined using cryo-EM, revealing a dimer of respiratory complexes III and IV, connected by di-heme cyt. cc subunit for electron transfer and creating a proton transfer pathway.
Article
Biochemistry & Molecular Biology
Fabian Falkenberg, Jade Rahba, David Fischer, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: The study characterized a high-alkaline subtilisin and found that it retains good activity under high salt concentrations and high temperatures. It also demonstrated high oxidative stability to hydrogen peroxide, making it a promising candidate for biotechnological applications.
Article
Microbiology
Fabian Falkenberg, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: This study used a data mining approach to find new subtilisin sequences in the family Bacillaceae and conducted a comprehensive survey of the S8 family. The analysis revealed previously uncharacterised groups and subgroups within the family, providing an excellent source for new subtilisin candidates.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Angela Kranz, Tino Polen, Christian Kotulla, Annette Arndt, Graziella Bosco, Michael Bussmann, Ava Chattopadhyay, Annette Cramer, Cedric-Farhad Davoudi, Ursula Degner, Ramon Diesveld, Raphael Freiherr von Boeselager, Kim Gaertner, Cornelia Gaetgens, Tobias Georgi, Christian Geraths, Sabine Haas, Antonia Heyer, Max Huennefeld, Takeru Ishige, Armin Kabus, Nicolai Kallscheuer, Larissa Kever, Simon Klaffl, Britta Kleine, Martina Kocan, Abigail Koch-Koerfges, Kim J. Kraxner, Andreas Krug, Aileen Krueger, Andreas Kueberl, Mohamed Labib, Christian Lange, Christina Mack, Tomoya Maeda, Regina Mahr, Stephan Majda, Andrea Michel, Xenia Morosov, Olga Mueller, Arun M. Nanda, Jens Nickel, Jennifer Pahlke, Eugen Pfeifer, Laura Platzen, Paul Ramp, Doris Rittmann, Steffen Schaffer, Sandra Scheele, Stephanie Spelberg, Julia Schulte, Jens-Eric Schweitzer, Georg Sindelar, Ulrike Sorger-Herrmann, Markus Spelberg, Corinna Stansen, Apilaasha Tharmasothirajan, Jan van Ooyen, Philana van Summeren-Wesenhagen, Michael Vogt, Sabrina Witthoff, Lingfeng Zhu, Bernhard J. Eikmanns, Marco Oldiges, Georg Schaumann, Meike Baumgart, Melanie Brocker, Lothar Eggeling, Roland Freudl, Julia Frunzke, Jan Marienhagen, Volker F. Wendisch, Michael Bott
Summary: Corynebacterium glutamicum is a major host for amino acid production and has been extensively studied in microbial biotechnology. This study presents a large compendium of transcriptional profiles, allowing researchers to investigate gene expression changes under different environmental conditions or genetic modifications. The compendium provides valuable insights into the understanding of C. glutamicum as a microbial cell factory.
Article
Microbiology
Paul Ramp, Christopher Pfleger, Jonas Dittrich, Christina Mack, Holger Gohlke, Michael Bott
Summary: myo-, scyllo-, and d-chiro-inositol are cyclic sugar alcohols with various biological functions and serve as carbon sources for microbes. Inositol dehydrogenases (IDHs) catalyze the oxidation of inositol and initiate its catabolism. Corynebacterium glutamicum encodes seven potential IDHs, and this study identified four of them as key enzymes involved in inositol catabolism. These IDHs exhibit distinct substrate preferences for different inositol isomers, and the study provides insights into substrate specificity through modeling and sequence alignments.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Christian K. Desiderato, Katharina M. Hasenauer, Sebastian J. Reich, Oliver Goldbeck, Lalaina Holivololona, Kirill Ovchinnikov, Alexander Reiter, Marco Oldiges, Dzung B. Diep, Bernhard J. Eikmanns, Christian U. Riedel
Summary: In this study, a Lactococcus petauri strain isolated from fermented balsam pear was found to produce a bacteriocin called garvicin Q. The gene cluster responsible for biosynthesis and secretion of garvicin Q was identified, and it was discovered that garvicin Q kills target bacteria by disrupting membrane integrity.
MICROBIAL CELL FACTORIES
(2022)
Article
Microbiology
Lea Sundermeyer, Graziella Bosco, Srushti Gujar, Melanie Brocker, Meike Baumgart, Dieter Willbold, Oliver H. Weiergraeber, Marco Bellinzoni, Michael Bott
Summary: In this study, we investigated the roles of GlnH and GlnX in the GlnH-GlnX-PknG-OdhI-OdhA signal transduction cascade in C. glutamicum, and confirmed their involvement through genetic, biochemical, and structural modeling approaches.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
HebaT'Allah Nasser, Bernhard J. Eikmanns, Mahmoud M. Tolba, Mohamed El-Azizi, Khaled Abou-Aisha
Summary: The results suggest that Gram-positive bacteria may be superior host strains for recombinant HA production over Gram-negative strains. The titers and molecular weight of HA produced by Bacillus megaterium were significantly higher than those obtained by the different E. coli host strains utilized in this study.
Article
Biotechnology & Applied Microbiology
Fabian Falkenberg, Leonie Voss, Michael Bott, Johannes Bongaerts, Petra Siegert
Summary: The aim of this study was to characterize four new subtilisins from halotolerant and halophilic microorganisms. The proteases showed activity over a wide pH range and exhibited high stability towards SDS and high concentrations of NaCl. These enzymes demonstrate potential for future biotechnological applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Chemical
Niklas Tenhaef, Alina Hermann, Moritz Fabian Mueller, Jonas Goertz, Jan Marienhagen, Marco Oldiges, Wolfgang Wiechert, Michael Bott, Andreas Jupke, Laura Hartmann, Sonja Herres-Pawlis, Stephan Noack
Summary: A simplified and scalable one-pot process was demonstrated for anaerobic production of succinic acid using a metabolically engineered Corynebacterium glutamicum strain. By optimizing the bioprocess, a succinic acid titer of 78 g L-1 and a yield of 1.41 mol(SA)mol(GLC)(-1) were achieved. Succinic acid was recovered from the fermentation broth and used for polybutylene bio-succinate synthesis using a biocompatible zinc catalyst, resulting in a final biopolymer comparable to those from commercial precursors.
CHEMIE INGENIEUR TECHNIK
(2023)
Article
Biotechnology & Applied Microbiology
Rico Zuchowski, Simone Schito, Friederike Neuheuser, Philipp Menke, Daniel Berger, Niels Hollmann, Srushti Gujar, Lea Sundermeyer, Christina Mack, Astrid Wirtz, Oliver H. Weiergraeber, Tino Polen, Michael Bott, Stephan Noack, Meike Baumgart
Summary: Using adaptive laboratory evolution, mutations that benefit the growth of synthetic co-cultures of Corynebacterium glutamicum were identified. Mutations involved in amino acid transport and expression-promoting mutations were discovered, leading to improved amino acid production. The study demonstrates the power of the synthetic co-culture approach for identifying non-obvious production traits and advancing amino acid production.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Paul Ramp, Christina Mack, Astrid Wirtz, Michael Bott
Summary: D-chiro-Inositol (DCI) is a potential drug candidate for insulin resistance and associated diseases. This study developed two production processes for DCI using Corynebacterium glutamicum as the host. The first process involved converting myo-inositol to DCI through several enzymatic reactions, resulting in a yield of 1.1 g/L. The second process utilized plant-derived enzymes to achieve a higher yield of 1.6 g/L by utilizing a novel route. The study also demonstrated the possibility of replacing the substrate with glucose, further increasing the production yield to 1.2 g/L.
METABOLIC ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Susana Matamouros, Thomas Gensch, Martin Cerff, Christian C. Sachs, Iman Abdollahzadeh, Johnny Hendriks, Lucas Horst, Niklas Tenhaef, Julia Tenhaef, Stephan Noack, Michaela Graf, Ralf Takors, Katharina Noeh, Michael Bott
Summary: The growth rate of bacteria is dependent on the number of active ribosomes and their translation elongation rate. This study analyzes the relationship between ribosome abundance, translation elongation rate, and growth rate in Corynebacterium glutamicum, a gram-positive bacterial species. Unlike Escherichia coli, C. glutamicum shows minimal change in ribosome abundance at low growth rates, while the translation elongation rate decreases significantly. Mathematical modeling suggests that the decline in translation elongation rate is due to a depletion of translation precursors.
NATURE COMMUNICATIONS
(2023)
