期刊
JOURNAL OF BIOTECHNOLOGY
卷 156, 期 3, 页码 214-217出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2011.07.040
关键词
Poly(glycolide); Poly(lactide); Lactate-polymerizing enzyme; Biobased plastic; Polyhydroxyalkanoate
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [23681015]
- Japan Bioindustry Association
- Association for the Progress of New Chemistry (Japan)
- Grants-in-Aid for Scientific Research [23681015] Funding Source: KAKEN
Glycolate(GL)-based polyesters were for the first time produced in the recombinant Escherichia coli fatty acid beta-oxidation pathway reinforcing mutant LS5218, using extracellularly added GL as a monomer precursor. Cells expressing a Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate synthase (PhaC1STQK) from Pseudomonas sp. 61-3, propionyl-CoA transferase from Megasphaera elsdenii and enoyl-CoA hydratase from Pseudomonas aeruginosa grown on GL and dodecanoate were found to produce novel copolymers of GL with 3-hydroxyalkanoates (3HAs) (C(4)-C(12)), P(GL-co-3HA), with a weight-average molecular weight of 34,000. The (1)H and (13)C NMR analyses of the copolymer revealed the incorporation of GL units into the polymer chain. This result demonstrates that PhaC1STQK polymerized glycolyl-CoA as a monomer substrate. Additionally, the novel lactate(LA)-based polyester P(LA-co-3HA) was produced by substituting GL with LA, indicating that the method is versatile and allows the production of a variety of biopolymers. (C) 2011 Elsevier B.V. All rights reserved.
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