期刊
JOURNAL OF BIOTECHNOLOGY
卷 150, 期 3, 页码 332-342出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2010.09.958
关键词
Spodoptera frugiperda; Cell density effect; Metabolic burden; Enzyme activity; Redox state; Baculovirus; Glutamate dehydrogenase
资金
- Project BACULOGENES [FP6 LHSB-CT-2006-037541]
- Project CLINIGENE-NoE [FP6 LSHB-CT-2004-018933]
- Fundacion Seneca-Murcia, Spain
- Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [SFRH/BD/36676/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/36676/2007] Funding Source: FCT
The scarcity of fundamental knowledge on the baculovirus-host cell interaction is a major drawback for the improvement of bioprocesses through Metabolic Engineering. After the first hours post-infection, the virus takes over the control of cellular machinery, leading to the repression of host gene expression and imposing a high metabolic burden to insect cells. Nevertheless, there is a lack of detailed data on the metabolic responses to infection, which are ultimately responsible for system productivity performance. In this work, a further insight into the central metabolism of Sf9 cells is achieved by a combined analysis of enzyme activities, cellular cofactors (ATP and NAD(P)(+)/NAD(P)H) and metabolic fluxes. Hexokinase and isocitrate dehydrogenase were identified as feasible limiting steps of metabolism; carbon and nitrogen metabolism enzymes were differentially regulated during batch cultures. Moreover, alterations occurring after infection demonstrated the importance of maintaining the energetic state of the cells for baculovirus replication, since ATP accumulated in a MOI-dependent way, and the glutamate dehydrogenase anaplerotic pathway was greatly activated. Altogether, cellular de-energization and stress responses are relevant factors in the metabolic burden imposed by infection. The implications for the improvement of bioprocess performance are discussed. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据