期刊
JOURNAL OF BIOTECHNOLOGY
卷 145, 期 1, 页码 47-53出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.09.007
关键词
Folate-PEG-g-TMC conjugate; Intracellular protein delivery; Folate; Targeting
资金
- National Science Foundation of China (NSFC) [30772668]
To develop a receptor-mediated intracellular delivery system that can transport therapeutic proteins to specific tumor cells, folate-poly(ethylene glycol)-grafted-trimethylchitosan (folate-PEG-g-TMC) was synthesized. Nano-scaled spherical polyelectrolyte complexes between the folate-PEG-g-TMC and fluorescein isothiocyanate conjugated bovine serum albumin (FITC-BSA) were prepared under suitable weight ratio of copolymer to FITC-BSA by ionic interaction between the positively charged copolymers and the negatively charged FITC-BSA. Intracellular uptake of FITC-BSA was specifically enhanced in SKOV3 cells (folate receptor over-expressing cell line) through folate receptor-mediated endocytosis compared with A549 cells (folate receptor deficient cell line). Folate-PEG-g-TMC shows promise for intracellular transport of negatively charged therapeutic proteins into folate receptor over-expressing tumor cells. (c) 2009 Elsevier B.V. All rights reserved.
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