Article
Microbiology
Marzieh Asadi, Mortaza Taheri-Anganeh, Maryam Ranjbar, Seyyed Hossein Khatami, Amir Maleksabet, Zohreh Mostafavi-Pour, Younes Ghasemi, Abdolkhalegh Keshavarzi, Amir Savardashtaki
Summary: This study evaluated the antibacterial activity of a novel exolysin containing an SH3b domain against MRSA. The results showed that the chimeric exolysin LYZ2-SH3b had a more inhibitory effect on MRSA compared to LYZ2 and vancomycin.
Article
Multidisciplinary Sciences
Jumpei Fujiki, Tomohiro Nakamura, Keisuke Nakamura, Keita Nishida, Yurika Amano, Yusaku Watanabe, Satoshi Gondaira, Masaru Usui, Masaru Shimizu, Kazuhiko Miyanaga, Shinya Watanabe, Tomohito Iwasaki, Kotaro Kiga, Tomoko Hanawa, Hidetoshi Higuchi, Teiji Sawa, Yasunori Tanji, Yutaka Tamura, Longzhu Cui, Hidetomo Iwano
Summary: The study assessed the biological stability and clinical potential of Staphylococcus virus Phi SA012. The virus showed a wide host range and efficient lytic activity. Experiments on mice demonstrated the ability of Phi SA012 to spread and accumulate in specific organs without causing inflammation. Additionally, the study highlighted the importance of appropriate dosing and administration periods for effective phage therapy.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Maria Kornienko, Dmitry Bespiatykh, Maja Malakhova, Roman Gorodnichev, Nikita Kuptsov, Egor Shitikov
Summary: Phage therapy is a hopeful approach to combat multidrug-resistant bacteria. However, it requires the isolation of new phages or the search for suitable phages in existing libraries. This study proposes a simple PCR method to differentiate between two families of Staphylococcus phages and eleven genera of Klebsiella phages, allowing for rapid screening and identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jun Song, Hongri Ruan, Li Chen, Yuqi Jin, Jiasan Zheng, Rui Wu, Dongbo Sun
Summary: SDQ, a specific lytic S. aureus bacteriophage, can effectively remove S. aureus biofilms. SDQ maintains its full activity under various environmental conditions and can be used in combination with detergents.
Article
Infectious Diseases
Eric T. Sumrall, Marloes Hofstee, Daniel Arens, Christian Roehrig, Susanne Baertl, Dominic Gehweiler, Mathias Schmelcher, Martin J. Loessner, Stephan Zeiter, R. Geoff Richards, T. Fintan Moriarty
Summary: The study demonstrates that the addition of enzybiotics can enhance the treatment of severe MRSA ODRI by rapidly killing the bacteria and targeting biofilm formation.
Article
Infectious Diseases
Kevin Simon, Wolfgang Pier, Alex Kruettgen, Hans-Peter Horz
Summary: The combination of Sb-1 phage and oxacillin showed enhanced antibacterial effects against most S. aureus isolates, with rare antagonism observed. This heterologous antimicrobial pair provides a robust approach for controlling MRSA.
Article
Biochemistry & Molecular Biology
Sha Liu, Karen Hon, George Spyro Bouras, Alkis James Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: The isolated Staphylococcus aureus phages showed significant bactericidal activity against the biofilm form and a broad host range, with low mutation rate, indicating potential for further development as a treatment alternative to antibiotics for Methicillin-Resistant Staphylococcus Aureus (MRSA) infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Elliot Whittard, James Redfern, Guoqing Xia, Andrew Millard, Roobinidevi Ragupathy, Sladjana Malic, Mark C. Enright
Summary: This study validates the potential of phage therapy for treating Staphylococcus aureus infections by examining the characteristics and host range of lytic S. aureus phages. Phages with the widest host range and effective killing abilities against various isolates were identified, showing promise as therapeutic candidates for future studies.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Fei Ma, Yu Ning, Qingqing Wan, Likou Zou, Yuntao Liu, Shujuan Chen, Jianlong Li, Zhen Zeng, Yong Yang, Hong Chen, Min Zhou, Shuliang Liu, Cheng Li, Aiping Liu
Summary: Bacteriophages LSA2308 and LSA2366 showed lytic activity against drug-resistant S. aureus, and combined with ampicillin achieved better clearance in LB broth. In skim milk, a concentration of 10^7 PFU/mL bacteriophages exhibited the most effective bactericidal efficacy against S. aureus strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Virology
Abby M. Korn, Andrew E. Hillhouse, Lichang Sun, Jason J. Gill
Summary: This study introduces three novel S. aureus jumbo phages: MarsHill, Madawaska, and Machias, isolated from swine production environments in the US. These phages belong to a new clade of S. aureus myophages with similar genome size and protein-coding genes, sharing characteristics with known jumbo phages of Bacillus sp. The phages possess genes encoding complete virion and nonvirion RNA polymerases, homing endonucleases, and a retron-like reverse transcriptase, with presumed uracil-substituted DNA hindering DNA sequencing. These phages also have the ability to transduce host plasmids and infect human S. aureus isolates, highlighting their potential impact on genetic diversity and horizontal gene transfer.
JOURNAL OF VIROLOGY
(2021)
Review
Microbiology
Kam Pou Ha, Andrew M. Edwards
Summary: Staphylococcus aureus demonstrates remarkable adaptability and resistance in infections, with its DNA repair process playing a crucial role in pathogen survival within host tissues. Studies suggest that targeting staphylococcal DNA repair could be a potential novel therapeutic strategy.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2021)
Article
Immunology
Ganga S. Moorthy, Rachel G. Greenberg, Chi D. Hornik, Cara Cassino, Parviz Ghahramani, Karan R. Kumar, Vance G. Fowler, Michael Cohen-Wolkowiez
Summary: Exebacase, a newly developed antimicrobial drug, shows potential as a safe and effective treatment option for severe infections, but further studies are needed to determine optimal dosing in children.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Allergy
Sha Liu, Yin Zhao, Andrew Hayes, Karen Hon, Guimin Zhang, Catherine Bennett, Hua Hu, John Finnie, Sandra Morales, Linda Shearwin, Alkis J. Psaltis, Keith Shearwin, Peter-John Wormald, Sarah Vreugde
Summary: Subinhibitory concentrations of protein synthesis inhibitor (PSI) antibiotics showed synergy when combined with phages in treating Staphylococcus aureus infections, sensitizing phage-resistant strains and promoting phage infection in vitro and in vivo. This suggests the potential use of phage-PSI antibiotic combination therapies for difficult-to-treat infections caused by phage-resistant S. aureus and MRSA strains.
Article
Biology
Salim Manoharadas, Mohammad Altaf, Abdulwahed Fahad Alrefaei, Naushad Ahmad, Shaik Althaf Hussain, Basel F. Al-Rayes
Summary: Researches have successfully designed an engineered antimicrobial protein to combat methicillin-resistant Staphylococcus aureus, showing strong activity. However, despite having a multimodular structure, the engineered protein failed to exhibit activity against a veterinary isolate of S. aureus.
Article
Food Science & Technology
Hyerim Kwak, Jinshil Kim, Sangryeol Ryu, Jaewoo Bai
Summary: Phage KMSP1, isolated from raw milk, was characterized as a lytic phage belonging to the Myoviridae family. It showed efficient antimicrobial activity against a broad spectrum of S. aureus strains, including MRSA. The presence of putative tail lysin with antimicrobial activity and the stability of KMSP1 under various conditions suggested its potential as a biocontrol agent.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Infectious Diseases
Salim Manoharadas, Mohammad Altaf, Naushad Ahmad, Abdulwahed Fahad Alrefaei, Basel F. F. Al-Rayes
Summary: The emergence of antibiotic resistance in enterococci is a global concern, and almost all enterococci show significant levels of resistance to various antibiotics. Therefore, it is crucial to develop new antibacterial agents against enterococci.
Article
Chemistry, Medicinal
Salim Manoharadas, Naushad Ahmad, Mohammad Altaf, Abdulwahed Fahad Alrefaei, Basel F. Al-Rayes
Summary: This study evaluated the activity of two engineered enzybiotics against a dual biofilm formed by Staphylococcus aureus and Enterococcus faecalis. The cocktail of proteins showed an additive effect in rapidly disrupting the preformed dual biofilm, resulting in over 90% dispersion within 3 hours of treatment. Furthermore, it effectively reduced bacterial cells embedded in the biofilm matrix by over 90% within the same time frame. This is the first successful use of an engineered enzybiotic cocktail to impede the structural integrity of a dual biofilm.
Article
Chemistry, Physical
Ana Carolina Ferreira Piazzi Fuhr, Yasmin Vieira, Marcos Leandro Silva Oliveira, Luis Felipe Oliveira Silva, Salim Manoharadas, Asad Nawaz, Guilherme Luiz Dotto
Summary: Mathematical models are important in data acquisition, but it is challenging to choose the most accurate model. This study uses statistical physics modeling and thermodynamic calculations to demonstrate patterns in adsorption processes and highlights the importance of understanding the physical meaning of each parameter.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Multidisciplinary Sciences
Bruna Silva de Farias, Francisca Zuchoski Rizzi, Eduardo Silveira Ribeiro, Patricia Silva Diaz, Tito Roberto Sant'Anna Cadaval Junior, Guilherme Luiz Dotto, Mohammad Rizwan Khan, Salim Manoharadas, Luiz Antonio de Almeida Pinto, Glaydson Simoes dos Reis
Summary: This study explores the fabrication of nanofibers using different types of gelatins, highlighting their potential to produce nanofibers with distinct physicochemical properties.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Salim Manoharadas, Basel F. Al-Rayes, Mohammed Abdulaziz M. Almuzaini, Yasser Muteq Almohammadi
Summary: This study successfully created a chimeric enzybiotic against MRSA and tested its efficiency in combination with antibiotics. This finding could pave the way for the development of similar peptides to be used in combination with conventional antibiotics.
Article
Food Science & Technology
Noor Akram, Muhammad Afzaal, Farhan Saeed, Adnan Ahmad, Ali Imran, Aftab Ahmed, Yasir Abbas Shah, Fakhar Islam, Suliman Yousef Alomar, Salim Manoharadas, Asad Nawaz
Summary: In this study, whey protein-based nanofibers were fabricated to encapsulate Lactobacillus rhamnosus. The nanofibers were blended using different ratios of PVA and WPI, and characterized for various properties. SEM and FTIR analysis showed successful loading of probiotics within the nanofibers, with strong bonding between the encapsulating materials. In vitro and thermal analyses demonstrated significant improvement in the survival of the encapsulated probiotics.
Article
Multidisciplinary Sciences
M. Lavanya, Rajapandiyan Krishnamoorthy, Mohammad A. Alshuniaber, Salim Manoharadas, Chella Perumal Palanisamy, Vishnu Priya Veeraraghavan, Selvaraj Jayaraman, Ponnulakshmi Rajagopal, Ramakrishnan Padmini
Summary: A gelatin-syringic acid/ZnO nanocomposite with antioxidant, anti-inflammatory, and anticancer activities was synthesized and characterized. The nanocomposite exhibited sustained drug release, high antioxidant ability, dose-dependent inhibition of protein denaturation, and non-toxicity in zebrafish. It also inhibited Hep G2 cell viability and increased oxidative stress caused by mitochondrial damage, leading to cell death. Overall, this study suggests that the gelatin-syringic acid/ZnO nanocomposite has potential for the treatment of hepatocellular carcinoma.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Nutrition & Dietetics
Aslam Khan, Zia Ul-Haq, Sheraz Fazid, Sadia Fatima, Nawshad Muhammad, Jawad Ahmed, Salim Manoharadas, Sher Zaman Safi, Ijaz Habib, Cecilia Garzon, Yasir Ihtesham, Fareeda Zahid, Fazal Dad, Tanimoune Mahamadou, Nicola M. Lowe
Summary: This study aimed to evaluate the effectiveness of ready-to-use Lipid-based Nutrient Supplement-Medium Quantity (LNS-MQ) on plasma micronutrient status, hemoglobin concentration, and anthropometric measurements. A total of 110 children aged 6 to 23 months were allocated to intervention and control groups. The intervention group received daily ration of LNS-MQ for one year and showed significant improvements in micronutrient status, hemoglobin concentration, and growth parameters.
FRONTIERS IN NUTRITION
(2023)
Article
Green & Sustainable Science & Technology
Larah Gondim Santos Paulino, Luisa Bataglin Avila, Caroline Costa Moraes, Mohammad Rizwan Khan, Salim Manoharadas, Glaydson Simoes dos Reis, Guilherme Luiz Dotto, Gabriela Silveira da Rosa
Summary: This study aims to enhance bilayer membranes by incorporating phytochemical compounds and natural extracts, resulting in improved mechanical and barrier properties. The addition of olive leaf extracts shows significant potential for utilization as wound dressings.
Article
Food Science & Technology
Sidra Akram, Aftab Ahmed, Muhammad Afzaal, Farhan Saeed, Ali Ikram, Aasma Asghar, Salim Manoharadas, Asad Nwaz, Degnet Teferi Asres
Summary: This study evaluated the effect of iron-rich Nutri bars on iron-deficient females and found that they could improve iron status in young women.
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2023)
Article
Multidisciplinary Sciences
Kalaiselvi Krishnamoorthy, Selvaraj Jayaraman, Rajapandiyan Krishnamoorthy, Salim Manoharadas, Mohammad A. Alshuniaber, Biba Vikas, Vishnu Priya Veeraraghavan
Summary: This study aims to develop an environmentally friendly method for isolating silver nanoparticles and investigate their pharmacological properties. The isolated silver nanoparticles using Argyreia nervosa plant leaves showed strong antioxidant, anti-inflammatory, and anti-diabetic activities, as well as significant antibacterial activity against selected pathogens. However, further in vivo research is needed to verify these findings and explore the potential of Argyreia nervosa-mediated silver nanoparticles as a viable drug.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Environmental Sciences
Rafaela Nogueira Fontoura da Silva, Paola de Azevedo Mello, Alice Penteado Holkem, Luis Felipe Oliveira Silva, Marcos Leandro Silva Oliveira, Asad Nawaz, Salim Manoharadas, Guilherme Luiz Dotto
Summary: The present research evaluated the use of grape stalk as a bioadsorbent for the adsorption of lanthanum and cerium. The results showed that grape stalk had a porous structure and exhibited good adsorption capacity for these rare earth elements. The study also demonstrated the potential of using grape residue as a promising bioadsorbent for recovering rare earth elements from phosphogypsum leachate.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Juliana Machado Nascimento dos Santos, Fabiola Balzan Dalla Nora, Katia da Boit Martinello, Melissa Gurgel Adeodato Vieira, Asad Nawaz, Luis Felipe Oliveira Silva, Salim Manoharadas, Guilherme Luiz Dotto
Summary: Grain cultivation and its impacts on the environment, specifically the generation of solid waste and the use of agrochemicals, have attracted much attention. This study proposes a novel approach to achieve self-sustainability in rice farming by reusing green silica waste as an adsorbent for treating pesticide-contaminated effluents. The carbonaceous material obtained from burning rice husks and subsequent treatment exhibited promising adsorption properties towards 2,4-dichlorophenoxyacetic acid (2,4-D) pesticide. The findings suggest that this material has the potential to contribute to the sustainable development of rice farming.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Horticulture
Muhammad Rizwan, Asad Nawaz, Sana Irshad, Salim Manoharadas
Summary: This study reveals the potential role of melatonin (MEL) in enhancing Cr stress tolerance in pepper seedlings. MEL supplementation significantly mitigated the phytotoxic effects of Cr on root morphology, gas exchange elements, and leaf photosynthetic capacity, and restored the growth of pepper seedlings.
SCIENTIA HORTICULTURAE
(2024)
Article
Materials Science, Textiles
R. Mariselvam, A. J. A. Ranjitsingh, Ponani Kaja Mideen, Salim Manoharadas, Rajapandiyan Krishnamoorthy, Mohammad A. Alshuniaber
Summary: This research focused on fixing Calendula officinalis plant flower dye on various natural fibers using mordants, resulting in different colors. Mordants can tonify the colors of Calendula officinalis dye and improve the light and water fastness of dyed natural fibers.
FIBRES & TEXTILES IN EASTERN EUROPE
(2023)
