期刊
JOURNAL OF BIOTECHNOLOGY
卷 144, 期 2, 页码 120-126出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jbiotec.2009.09.005
关键词
ATP synthase inhibitor; Candida glabrata; EGFP; INH1; Glycolytic pathway
资金
- National Outstanding Youth Foundation of China [20625619]
- National Basic Research Program of China (973) [2007CB714303]
- National High-Tech Research and Development Program of China (863) [2006AA02Z201, 2007AA100402]
- National Natural Science Foundation of China [30670066, 20706025]
The aim of this study is to establish a controllable intracellular ATP content regulation system applied to the enhancement of pyruvate production in Torulopsis glabrata. The INH1 gene, which encodes a F0F1-ATPase inhibitor from Saccharomyces cerevisiae, was expressed under a copper ion inducible promoter in the pyruvate producer Torulopsis glabrata CCTCC M202019. The resultant strain was designated as T. glabrata INH1. The induction efficiency was measured by the inducible expression of an enhanced green fluorescence protein. The copper inducible INH1 gene could control the intracellular ATP content (24 h) in an extensive range between 0.192 mmol/mg protein and 0.642 mmol/mg protein in a flask culture. With T. glabrata INH1, induction with 30 mu M of Cu2+ at 12 h in a 3 L fermentor improved pyruvate yield from glucose on biomass by 29% and its yield by 20%, respectively. (C) 2009 Elsevier B.V. All rights reserved.
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