Article
Chemistry, Medicinal
Yumei Feng, Yuanmin Zhu, Zhendong Bao, Bohan Wang, Tingting Liu, Huihui Wang, Tianyi Yu, Ying Yang, Longjiang Yu
Summary: This study demonstrated that overexpression of endogenous glucose-6-phosphate dehydrogenase (G6PD) gene in Schizochytrium sp. H016 increased the availability of NADPH, resulting in higher yield of docosahexaenoic acid (DHA) and lipid accumulation. These findings uncovered the beneficial effects of NADPH on PUFA synthesis in Schizochytrium sp. H016 and provided a promising regulatory strategy for large-scale DHA production.
Article
Biology
Wang Ma, Mengzhen Liu, Zixu Zhang, Yingshuang Xu, Pengwei Huang, Dongsheng Guo, Xiaoman Sun, He Huang
Summary: In this study, a MetE-like complex was described for the first time, which can increase the content of EPA in Schizochytrium sp. and thus enhance its production. Lipidomics analysis revealed that EPA is more inclined to be stored as triglyceride. These findings suggest that Schizochytrium sp. has great potential for co-production of EPA and DHA.
COMMUNICATIONS BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Yu-Lei Jia, Fei Du, Fang-Tong Nong, Jin Li, Peng-Wei Huang, Wang Ma, Yang Gu, Xiao-Man Sun
Summary: This study investigated the functions of ORFA, ORFB, ORFC, and their individual functional domain genes in fatty acid synthesis by heterologous expression in Yarrowia lipolytica. The results showed that the expression of these genes or functional domains led to an increase in very long-chain PUFA content. Transcriptomic analysis revealed the upregulation of the tricarboxylic acid cycle and pentose phosphate pathway, and the downregulation of triacylglycerol biosynthesis, fatty acid synthesis pathway, and fl-oxidation. These findings provide a theoretical basis for understanding the function of PKS in fatty acid synthesis and the mechanism of PUFA biosynthesis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Yu-Lei Jia, Fei Du, Fang-Tong Nong, Jin Li, Peng-Wei Huang, Wang Ma, Yang Gu, Xiao-Man Sun
Summary: This study investigated the functions of ORFA, ORFB, ORFC, and their individual functional domain genes in fatty acid synthesis through heterologous expression in Yarrowia lipolytica. The results showed that the expression of these genes and domains led to an increase in very long-chain polyunsaturated fatty acid content. Transcriptomic analysis revealed upregulation of the tricarboxylic acid cycle and pentose phosphate pathway, and downregulation of triacylglycerol biosynthesis, fatty acid synthesis pathway, and beta-oxidation. These findings provide a theoretical basis for understanding the function of polyketide synthase in fatty acid synthesis and the mechanism of polyunsaturated fatty acid biosynthesis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Guoxiang Chi, Yiyuan Xu, Xingyu Cao, Zhipeng Li, Mingfeng Cao, Yusuf Chisti, Ning He
Summary: Dietary consumption of omega-3 long-chain polyunsaturated fatty acids (omega-3 LC-PUFA), especially docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has various health benefits. Traditional sources of these fatty acids, such as fish oil, are limited and of variable quality. Therefore, alternative sources are being sought. DHA derived from certain marine protists (heterotrophic thraustochytrids) has been commercially produced for high-value dietary use, but it is too expensive for use in aquaculture feeds. Aquaculture is currently dependent on wild-caught fish oil as a source of omega-3 LC-PUFA, which restricts sustainable expansion. Schizochytrium spp. (now Aurantiochytrium spp.) is of particular interest as it has shown to be a good producer of DHA and EPA. Various strategies have been developed to improve the production and economics of omega-3 PUFA in Schizochytrium spp.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Xiao Han, Yana Liu, Zhi Chen
Summary: In this study, LipR, a C2H2 zinc finger protein, was found to be an essential repressor of DHA and saturated fatty acid biosynthesis in Schizochytrium sp. LipR directly inhibits the transcription of PUFA synthase and fas genes through binding to a specific recognition sequence in the upstream regions of these genes. LipR also represses the expression of genes related to precursor and NADPH levels, as well as the triacylglycerol storage rate. This study provides insights into the regulatory mechanisms of DHA and lipid production in Schizochytrium sp. and suggests that manipulating the lipR gene could enhance the accumulation of polyunsaturated fatty acids and lipids in thraustochytrids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Lei Jia, Yu-Zhou Wang, Fang-Tong Nong, Wang Ma, Peng-Wei Huang, Xiao-Man Sun
Summary: This study identified and verified the presence of fatty acid desaturase genes in Schizochytrium. By genetically engineering these genes, the researchers were able to regulate the content of polyunsaturated fatty acids in the algae, providing valuable insights for optimizing the fatty acid composition of Schizochytrium in the future.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Green & Sustainable Science & Technology
Tae-Hyoung Kim, Kyungho Lee, Baek-Rock Oh, Mi-Eun Lee, Minji Seo, Sheng Li, Jae-Kon Kim, Minkee Choi, Yong Keun Chang
Summary: This study demonstrates an economically beneficial process for coproducing biojet fuel and high-value polyunsaturated fatty acids from Schizochytrium sp. ABC101 microalgae. The separation and conversion of PUFAs into biojet fuel and high-value fatty acids have a great commercial potential.
Article
Nutrition & Dietetics
Melanie Gsoellpointner, Margarita Thanhaeuser, Fabian Eibensteiner, Robin Ristl, Bernd Jilma, Renate Fuiko, Sophia Brandstetter, Angelika Berger, Nadja Haiden
Summary: This study analyzed the intake of polyunsaturated fatty acids (PUFAs) during complementary feeding (CF) in very low birth weight (VLBW) infants and its impact on neurodevelopment. The results showed that higher intake of total PUFAs, docosahexaenoic acid (DHA), and arachidonic acid (AA) was associated with improved cognitive and motor function at 12 months of age. However, the dietary intake of AA and DHA did not meet the recommended levels, highlighting the need to increase PUFA, DHA, and AA intake during CF for better neurodevelopmental outcomes in VLBW infants.
Article
Biotechnology & Applied Microbiology
Yanli Man, Yuting Zhang, Jiayi Jiang, Quanyu Zhao, Lujing Ren
Summary: This study compared four different dehydratase (DH) domains from different strains and analyzed their expression in Escherichia coli. The results showed that DH1,2 could enhance the percentage of unsaturated fatty acids, while DH3 benefited for saturated fatty acid biosynthesis. Additionally, DH from different gene clusters do have specific substrate preference.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Environmental Sciences
Shuangfei Li, Boyu Wang, Liangxu Liu, Yingjie Song, Chuhan Lv, Xingyu Zhu, Yanqing Luo, Christopher H. K. Cheng, Huapu Chen, Xuewei Yang, Tao Li
Summary: The study demonstrates that adding Aurantiochytrium sp. to diets can significantly improve the growth performance and immune response of Trachinotus ovatus. With increased microalgae content, the growth rate and feed utilization efficiency of fish were enhanced, along with increased fatty acid content in blood and tissues, resulting in improved immunity and disease resistance in fish.
FRONTIERS IN MARINE SCIENCE
(2021)
Review
Nutrition & Dietetics
Hans Demmelmair, Berthold Koletzko
Summary: High obesity rates around the world point to an urgent need for effective obesity prevention measures. Animal studies suggest that the effects of essential polyunsaturated fatty acids on adipogenesis may be mediated through various mechanisms. Divergent findings from human clinical trials and observational studies suggest that recommendations for humans regarding PUFA and obesity risk are not yet possible.
Article
Oncology
Safiye Aktas, Pinar Ercetin, Zekiye Altun, Mehmet Kantar, Nur Olgun
Summary: In this in vitro study, it was found that EPA did not promote proliferation in most cancer cells and did not affect the cytotoxic effects of chemotherapy drugs. Additionally, it was observed that EPA had some proliferative effects on fibroblasts, but did not reduce the toxicity of chemotherapy drugs on these cells.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ines Drenjancevic, Jan Pitha
Summary: In addition to proven medications, omega-3 polyunsaturated fatty acids (n-3 PUFAs) are considered to have additive effects on cardiovascular health. They influence the cardiovascular system through various mechanisms, including effects on metabolism, inflammation, thrombosis, and direct effects on cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xuechao Hu, Yangyang Luo, Yanli Man, Xiuyang Tang, Zhiqian Bi, Lujing Ren
Summary: This study investigated the response mechanism of Schizochytrium sp. to temperature stress using transcriptomic and lipidomic approaches. The results showed that high or low temperature inhibited cell growth and affected lipid biosynthesis. Low temperature induced more saturated fatty acids, while high temperature enlarged cell size. Phospholipids were found to be more sensitive to temperature change. The transcriptome analysis revealed the up-regulation of desaturase at low temperature and the up-regulated heat shock proteins at high temperature.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Genetics & Heredity
Yue Xie, Sen Wang, Shuangyang Wu, Shenghan Gao, Qingshu Meng, Chengdong Wang, Jingchao Lan, Li Luo, Xuan Zhou, Jing Xu, Xiaobin Gu, Ran He, Zijiang Yang, Xuerong Peng, Songnian Hu, Guangyou Yang
Summary: This study reveals the host adaptation of Baylisascaris schroederi to the giant panda and provides insights into the evolutionary trajectory of host-shift events in ascaridoid parasite lineages. The specific adaptations of B. schroederi to survive in the gut of giant pandas, such as increasing cuticle thickness and efficient utilization of host nutrients, are characterized. The findings of this study will aid in the development of prevention and treatment measures to protect giant panda populations from roundworm parasitism.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Fisheries
Guo Guangxin, Kexin Li, Qianhui Zhu, Chunyan Zhao, Chao Li, Zilong He, Songnian Hu, Yichao Ren
Summary: Plant extract oregano oil and probiotics can improve growth performance and disease resistance in cultured fish by modulating the gut microbiota and immune-related gene expression. The use of oregano oil or Bacillus coagulans as dietary additives shows potential health benefits for fish, enhancing growth and immunity through microbiota modulation.
Article
Biochemistry & Molecular Biology
Qinglan Sun, Chang Shu, Wenyu Shi, Yingfeng Luo, Guomei Fan, Jingyi Nie, Yuhai Bi, Qihui Wang, Jianxun Qi, Jian Lu, Yuanchun Zhou, Zhihong Shen, Zhen Meng, Xinjiao Zhang, Zhengfei Yu, Shenghan Gao, Linhuan Wu, Juncai Ma, Songnian Hu
Summary: This research established an evaluation and prewarning system to monitor the genomic variation risks of SARS-CoV-2. It analyzed the effects of known and virtual variations on viral properties from the perspectives of genomics and structural biology, and verified the effectiveness of these characteristics for risk prediction using an artificial intelligence algorithm.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Sen Wang, Shenghan Gao, Jingyi Nie, Xinyu Tan, Junhua Xie, Xiaochun Bi, Yan Sun, Sainan Luo, Qianhui Zhu, Jianing Geng, Wanfei Liu, Qiang Lin, Peng Cui, Songnian Hu, Shuangyang Wu
Summary: This study updated the 93-11 genome assembly to a gap-less sequence using various methods and techniques. The comparison with the reference genome confirmed the mapping of sequencing data from indica rice to the 93-11 genome. The study also identified gene expression patterns related to seed development, alternative splicing of starch synthesis-related genes, and genomic variations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Li Wang, Tao Yu, Yaxin Zhu, Yingfeng Luo, Fan Dong, Xuemei Lin, Wenzhong Zhao, Zilong He, Songnian Hu, Zhiyang Dong
Summary: This study demonstrates that microbial networks on the scalp and hair surface of dandruff patients are less stable compared to their healthy counterparts, with lower node degree and more positive and stronger links. Lactobacillus bacteria have extensive interactions with other bacteria or fungi on the scalp and hair surface.
Article
Microbiology
Na Liu, Xin Zheng, Qianhui Zhu, Zilong He, Songnian Hu
Summary: Acinetobacter baumannii is a common hospital pathogen that has been increasing in strains carrying the NDM-1 gene. Through investigating 186 strains with the NDM-1 gene, this study aims to provide a theoretical basis for preventing the dissemination of NDM-1 in A. baumannii.
CURRENT MICROBIOLOGY
(2022)
Article
Immunology
Zhaoqi Zhang, Elhusseny A. Bossila, Ling Li, Songnian Hu, Yong Zhao
Summary: This study used RNA-seq data to analyze transcriptome profiles and cell metabolism during monocyte development, revealing important genes and pathways involved in this process. The findings provide valuable insights into the dynamics of monocyte development in bone marrow.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Na Liu, Danping Liu, Kexin Li, Songnian Hu, Zilong He
Summary: This study utilized Staphylococcus aureus as a model organism to investigate the driving factors influencing bacterial pan-genome composition. The research found that the sequence type plays a significant role in the dynamic distribution of bacterial genes. A new classification method and marker gene combination were proposed, providing insights for the classification of Staphylococcus aureus.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Si Yu, Xiaomeng Ge, Hui Xu, Bei Tan, Bowen Tian, Yujie Shi, Yimin Dai, Yue Li, Songnian Hu, Jiaming Qian
Summary: This study aimed to assess the gut microbiome and mycobiome in IBD patients with CDI. The results showed that IBD patients with CDI had decreased bacterial alpha diversity and significant differences in bacterial and fungal beta diversity compared to healthy individuals and IBD patients without CDI. Specific bacterial and fungal taxa were overrepresented in IBD patients with CDI. These findings provide insights into the pathological process and potential strategies for diagnosis and treatment in this subset of patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Xin Ma, Kexin Li, Fang Li, Jing Su, Weiwei Meng, Yanming Sun, Hui Sun, Jiazheng Sun, Yonghe Yuan, Yujia Lin, Songnian Hu, Xuefang Xu, Zilong He
Summary: Tracing foodborne botulism events quickly and accurately is crucial. This study used whole-genome sequencing and established universal core gene markers to successfully trace the relationship between clinical and food C. botulinum isolates, and identified three outbreaks.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yahui Guo, Xiaojie Yu, Jixiao Wang, De Hua, Yuanhai You, Qingbo Wu, Qinglong Ji, Jianzhong Zhang, Liefei Li, Yuan Hu, Zhonghui Wu, Xiaoyue Wei, Lianqun Jin, Fanliang Meng, Yuhua Yang, Xiaofeng Hu, Lijin Long, Songnian Hu, Heyuan Qi, Juncai Ma, Wenwen Bei, Xiaomei Yan, Haibin Wang, Zilong He
Summary: ST7 Staphylococcus aureus, which is highly prevalent in humans, pigs, and food in China, has rarely been reported to cause staphylococcal food poisoning (SFP). However, a recent outbreak of SFP caused by ST7 S. aureus strains occurred in two kindergartens in Hainan Province, China. Whole-genome sequencing analysis revealed the genetic characteristics and phylogenetic relationships of these SFP strains and provided insights into the potential risk of this emergent ST7 clone for SFP.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Zhi-Lin Ling, Bin Cao, Song-Nian Hu, Jia-Ning Geng, Fei Liu, Dong-Mei Liu, Rui-Lin Zhao
Summary: Agaricus sinodeliciosus, a rare wild edible mushroom from northwest China, shows unique genome changes and activates specific genes for saline-alkali tolerance. Transcriptomic analyses reveal the importance of carbon and nitrogen utilization, cell stability, and fruit body formation under mildly alkaline conditions. This research enhances our understanding of the genomic evolution and mechanisms of A. sinodeliciosus in tolerance to saline-alkali environments.
MICROBIAL GENOMICS
(2023)
Article
Microbiology
Qianhui Zhu, Shenghan Gao, Binghan Xiao, Zilong He, Songnian Hu
Summary: In this study, a novel plasmid prediction tool called Plasmer was proposed, which combines the advantages of shared k-mers and genomic features. Plasmer can predict plasmids in multiple species and has achieved high accuracy on contigs above 500 bp. It outperforms existing methods in terms of performance and has balanced sensitivity and specificity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Chang Shu, Qinglan Sun, Guomei Fan, Kesheng Peng, Zhengfei Yu, Yingfeng Luo, Shenghan Gao, Juncai Ma, Tao Deng, Songnian Hu, Linhuan Wu
Summary: The VarEPS-Influ database is a risk assessment system for influenza virus variations that comprehensively evaluates various aspects of mutations, including physical and chemical properties, structural features, receptor affinity, antibody binding capacity, etc., and considers factors such as time and spatial distribution and host species distribution. It accurately evaluates and predicts the risk of influenza virus variations.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Agriculture, Multidisciplinary
L. Zeng, X. Tan, S. Hu, Y. Luo
Summary: This study investigated the complexity and characteristics of the rice nuclear transcriptome. The expression of transposable element-related genes was found to be higher in the nucleus compared to protoplasts. Additionally, nuclear-enriched long non-protein coding RNAs were found to be co-expressed with nuclear-located RNA binding proteins. These findings provide valuable insights into the functional characterization of nuclear-expressed transposable element-related genes and lncRNAs.
JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS
(2022)
