Review
Virology
Aaron Pavel Rodriguez-Hernandez, Daniel Martinez-Flores, Adolfo Cruz-Resendiz, Teresa Padilla-Flores, Rodrigo Gonzalez-Flores, Kenia Estrada, Alicia Sampieri, Aldo Roman Camacho-Zarco, Luis Vaca
Summary: Baculoviridae is a large family of viruses that infect arthropods. Recombinant baculoviruses have various applications, including large-scale protein production in insect cell cultures and protein display in medicine. This review analyzes different strategies for protein and peptide display on recombinant baculoviruses and provides examples of proteins displayed. The commercially available systems for recombinant baculovirus production and display are briefly discussed, along with the future prospects of this emerging technology.
Article
Virology
Maria Victoria Nugnes, Alexandra Marisa Targovnik, Adria Mengual-Marti, Maria Victoria Miranda, Carolina Susana Cerrudo, Salvador Herrero, Mariano Nicolas Belaich
Summary: This study utilized CRISPR/Cas9 technology to pseudogenize the p74 gene of AcMNPV, revealing that the amino terminal sector of P74 is sufficient to restore virus infectivity, providing insight for enhancing viral activity and producing improved biopesticides.
Article
Virology
Hao Zheng, Yong Pan, Xiong Wang, Weibin Tian, Lunguang Yao, Jingchen Sun
Summary: By screening for suitable promoters, utilizing the envelope display effect of INVASIN and VSVG proteins, and constructing a recombinant baculovirus vector, the study was able to improve the efficiency and effectiveness of the baculovirus display system, providing a foundation for further development.
Review
Biotechnology & Applied Microbiology
Haikun Liu, Geoffrey Pietersz, Karlheinz Peter, Xiaowei Wang
Summary: This article discusses the pathological mechanism of atherosclerosis and atherothrombosis, and the latest advancements in targeted therapy using drug delivery and nanoparticle carrier systems. It also explores the limitations and future perspectives of this treatment approach.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Randall A. Meyer, Sarah Y. Neshat, Jordan J. Green, Jose Luis Santos, Anthony D. Tuesca
Summary: mRNA gene therapy has the potential to enable various therapeutic applications, but its successful clinical translation has been limited due to inadequate understanding of organ-specific protein expression. Studies have shown that carrier material properties and routes of administration are important factors in mRNA therapeutic expression profiles. Understanding trends in tissue-specific mRNA delivery materials can unlock the full potential of therapeutic mRNA medicines.
MATERIALS TODAY ADVANCES
(2022)
Article
Chemistry, Multidisciplinary
Dongxiao Yin, Mengjie Zhang, Jiaxin Chen, Yuanyu Huang, Dehai Liang
Summary: This study designed a series of cationic peptides with the same charge density but varying hydrophobicity and capacity to form hydrogen bonds, and investigated their ability to form complexes with siRNA and their behavior in the presence of serum. The hydrophobic interaction controls the complexation between peptide and siRNA, while hydrogen bonds are responsible for binding peptides to serum components.
CHINESE CHEMICAL LETTERS
(2021)
Article
Microbiology
Lionel Galibert, Aurelien Jacob, Adrien Savy, Yohann Dickx, Delphine Bonnin, Christophe Lecomte, Lise Rivollet, Peggy Sanatine, Marjorie Boutin Fontaine, Christine Le Bec, Otto-Wilhelm Merten
Summary: Large-scale manufacturing of rAAV remains a challenge, with the Monobac system showing higher yields of rAAV vectors and lower DNA contamination compared to dual baculovirus systems.
Article
Multidisciplinary Sciences
Sean A. Dilliard, Qiang Cheng, Daniel J. Siegwart
Summary: SORT nanoparticles have been developed to deliver genetic medicines to non-liver tissues by incorporating a supplemental SORT molecule and recruiting specific proteins to the nanoparticle surface, bypassing the liver accumulation barrier typically seen with traditional LNPs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Sean A. Dilliard, Qiang Cheng, Daniel J. Siegwart
Summary: By adding specific SORT molecules, SORT nanoparticles expand the therapeutic applications of genetic medicines by delivering mRNA and gene editing systems to non-liver tissues. The organ-targeting properties of SORT nanoparticles are controlled by the chemical nature of the added SORT molecules, with an endogenous targeting mechanism involving multiple processes, from desorption of PEG lipids to binding of specific proteins to the nanoparticle surface, and subsequent interactions with receptors highly expressed in specific tissues.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Chemistry, Physical
Vivekanandhan Karthik, Shanmugam Poornima, Arumugam Vigneshwaran, Daniel Paul Raj Dharun Daniel Raj, Ramasamy Subbaiya, Sivasubramanian Manikandan, Muthupandian Saravanan
Summary: Nanoarchitectonics is the process of fabricating functional materials by combining nanotechnology with other fields including supramolecular chemistry, self-organization, chemical synthesis and biotechnology. Applications of nanoarchitectonics are diverse, with potential for use in sensors, drug delivery and gene delivery. Biofunctionalized nanoparticles offer improved stability and efficiency, making them promising candidates for targeted delivery systems.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Immunology
Chih-Chi Liao, Chih-Hsuan Tsai, Huei-Ru Lo, Pey-Ru Lin, Chang-Chi Lin, Yu-Chan Chao
Summary: Scrub typhus is a fatal epidemic in the Asia-Pacific region, often misdiagnosed, and the lack of accurate detection methods poses a global health threat. Researchers successfully expressed O. tsutsugamushi antigens on insect cell surfaces and developed a cell-based ELISA detection method with high accuracy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, Yunhua Liu, Sheng Liu, Wenquan Ou, Alisa White, Samantha Stewart, Katherine H. R. Tkaczuk, Lee M. Ellis, Jun Wan, Xiongbin Lu, Xiaoming He
Summary: Colon and rectal cancers are leading causes of cancer-related deaths in the United States. Genomic analyses reveal the frequent hemizygous deletion of TP53 gene, an important tumor suppressor, in both cancers. Targeting TP53 directly has been unsuccessful, but the co-deletion of POLR2A gene with TP53 in these cancers suggests RNA interference with POLR2A siRNA as a potential strategy. However, efficient delivery of siRNA into the cytosol is a major challenge. To overcome this barrier, pH-responsive MetC-nanoparticles with a bomb effect are developed for effective cytosolic delivery of POLR2A siRNA, enhancing therapeutic efficacy for TP53-deficient cancer.
Review
Chemistry, Multidisciplinary
Deepak Kumar Sahel, Lalitkumar K. Vora, Aishwarya Saraswat, Saurabh Sharma, Jasmin Monpara, Anisha A. D'Souza, Deepakkumar Mishra, Kamatham Pushpa Tryphena, Satoru Kawakita, Shahid Khan, Mohd Azhar, Dharmendra Kumar Khatri, Ketan Patel, Raghu Raj Singh Thakur
Summary: CRISPR and Cas9, initially discovered as an immune system in bacteria and archaea, are now widely used as biotechnological tools for genetic and nongenetic disease treatment. Clinical trials mainly adopt ex vivo strategies, but in vivo delivery methods for CRISPR components are still under preclinical surveillance.
Article
Chemistry, Medicinal
Sabrina Schaly, Paromita Islam, Ahmed Abosalha, Jacqueline L. Boyajian, Dominique Shum-Tim, Satya Prakash
Summary: This study demonstrates that encapsulating a recombinant baculovirus expressing VEGFA in chitosan-coated alginate hydrogels enhances cell migration, angiogenesis, and wound healing, while also providing antimicrobial properties and sustained delivery of the virus.
Review
Pharmacology & Pharmacy
Rama Kashikar, Arun K. Kotha, Saurabh Shah, Paras Famta, Shashi Bala Singh, Saurabh Srivastava, Mahavir Bhupal Chougule
Summary: RNA binding proteins play a crucial role in RNA regulation, and their abnormal expression can lead to disease progression. This article discusses the latest findings in cancer therapy and diagnosis targeting RNA binding proteins, as well as the application of various interventions and the development of nanoparticle delivery technologies.
ADVANCED DRUG DELIVERY REVIEWS
(2022)