Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Surabhi Mangal, Sanjay Chhibber, Vasundhara Singh, Kusum Harjai
Summary: The present study investigated the antimicrobial and antivirulence potential of guaiacol against Pseudomonas aeruginosa. The results demonstrated that guaiacol in combination with ciprofloxacin exhibited synergistic effects, reducing virulence factors and biofilm formation. Guaiacol also disrupted the bacterial membrane, leading to the release of cellular material.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Infectious Diseases
Mario Gajdacs, Zoltan Barath, Krisztina Karpati, Dora Szabo, Donatella Usai, Stefania Zanetti, Matthew Gavino Donadu
Summary: The study found a relationship between biofilm formation and expression of virulence factors with multidrug resistance in Pseudomonas aeruginosa. However, there were no significant differences in virulence factor expression between multidrug-resistant and non-multidrug-resistant isolates, and no correlations were seen between the rate of biofilm formation, pigment production, or motility. Understanding the interplay between drug resistance mechanisms, biofilm formation, and virulence is crucial for managing chronic bacterial infections.
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Microbiology
Jens Bo Andersen, Kasper Norskov Kragh, Louise Dahl Hultqvist, Morten Rybtke, Martin Nilsson, Tim Holm Jakobsen, Michael Givskov, Tim Tolker-Nielsen
Summary: A decade of research has shown the importance of c-di-GMP in bacteria, with high levels associated with biofilm formation and low levels with planktonic bacteria. Inducing specific phosphodiesterases in P. aeruginosa can lead to biofilm dispersal.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Physics, Multidisciplinary
Bikash Bhattarai, Gordon F. Christopher
Summary: Current research in the treatment of biofilm infections has focused on dispersing bacteria from the extracellular polymeric substance (EPS) surrounding them to enhance antimicrobial treatments. Glycoside hydrolases (GHs), which break down EPS polysaccharides, have been found to promote dispersal in biofilms. This study explores the impact of three GHs on the mechanical stability of Pseudomonas aeruginosa biofilms using passive microrheology, and found that treatment with all three GHs resulted in less elastic and stiffer biofilms, indicating changes in mechanical stability may contribute to effective dispersal.
FRONTIERS IN PHYSICS
(2023)
Article
Agricultural Engineering
Qian Feng, Laiwei Luo, Xindi Chen, Kaijie Zhang, Fang Fang, Zhaoxia Xue, Chao Li, Jiashun Cao, Jingyang Luo
Summary: Exogenous AHLs such as C6-HSL and C8-HSL were found to promote biofilm formation of Pseudomonas aeruginosa significantly, enhancing adhesive ability, bacterial motility, and metabolic activities. These AHLs also upregulated the expression of microbial functional genes involved in biofilm formation, providing valuable insights for biofilm regulation in wastewater treatment using biofilm technology.
BIORESOURCE TECHNOLOGY
(2021)
Article
Microbiology
Divakar Badal, Abhijith Vimal Jayarani, Mohammad Ameen Kollaran, Deep Prakash, P. Monisha, Varsha Singh
Summary: Pseudomonas aeruginosa, a human pathogen, demonstrates swarming behavior driven by ethanol as a signaling molecule under nutrient-limiting conditions. This collective behavior is regulated by specific genes and response regulators, and also involves interactions with other microbial species. Ethanol acts as an important environmental cue for the chemotactic response of P. aeruginosa to induce swarming as a foraging behavior.
Article
Microbiology
Mizuki Kanno, Takuya Shiota, So Ueno, Minato Takahara, Keisuke Haneda, Yuhei O. Tahara, Masaki Shintani, Ryoma Nakao, Makoto Miyata, Kazuhide Kimbara, Hiroyuki Futamata, Yosuke Tashiro
Summary: Membrane vesicles (MVs) are small spherical structures produced by bacteria, playing important roles in bacterial biology and host immune modulation. This study revealed enhanced MV formation in Pseudomonas aeruginosa biofilms, which is closely related to biofilm matrix synthesis and surface sensing.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
David M. Hershey
Summary: Recent studies have shown the important role of the flagellum in promoting the motile-sessile transition and its regulatory mechanisms related to surface colonization. The flagellum promotes the sessile state by increasing the production of c-di-GMP, and can activate c-di-GMP production through multiple pathways.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Immunology
Siying Lin, Shuzhen Chen, Li Li, Huiluo Cao, Ting Li, Ming Hu, Lisheng Liao, Lian-Hui Zhang, Zeling Xu
Summary: This study characterized a uropathogenic Pseudomonas aeruginosa strain, PA_HN002, and found that its high intracellular c-di-GMP content contributed to enhanced biofilm formation. Analysis of the c-di-GMP metabolizing enzymes in PA_HN002 revealed multiple mutations in phosphodiesterases (PDEs), which resulted in a shifted expression profile and elevated intracellular c-di-GMP levels, promoting biofilm production.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Letter
Biochemical Research Methods
Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, a novel transcription-associated fluorescence-activated droplet sorting method was developed for specific targeting of di-RL hyperproducers. Using this method, ten higher di-RL producing microorganisms were screened out, which showed significantly increased di-RL yield compared to the model strain. This study provides a rapid, reliable screening method for di-RL production and offers a way to measure di-RL yield in populations of droplets containing different environmental colonies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Microbiology
Tian Zhou, Jiahui Huang, Zhiqing Liu, Zeling Xu, Lian-hui Zhang
Summary: Pseudomonas aeruginosa, a major cause of nosocomial infection, exhibits great adaptive ability under diverse environmental conditions, regulated by multiple signaling systems such as the two-component system (TCS) including FleS/FleR. This study identified key residues and regulatory roles of FleS/FleR in virulence-related traits and revealed their involvement in biofilm formation and flagellum biosynthesis. The findings provide insights into the mechanisms of regulation by FleS/FleR in P. aeruginosa.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Fatma Allam, Mohamed Elnouby, Soraya A. Sabry, K. M. El-Khatib, Dalia E. El-Badan
Summary: This study isolated a local electroactive strain of Pseudomonas aeruginosa FA17 and optimized the composition of artificial wastewater media to investigate its electrochemical behavior, biofilm formation, and rhamnolipid production. Factors affecting current generation, biofilm formation, and rhamnolipid production by FA17 were identified, potentially leading to future development of power production by microbial electrochemical cells using the electroactive strain.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biochemistry & Molecular Biology
Alfonso Oliva, Scheherezade Garcia-Carrillo, Antonio Ortiz, Francisco J. Aranda, Jose A. Teruel
Summary: The interaction between dirhamnolipid biosurfactant and calcium ATPase from sarcoplasmic reticulum was studied, revealing that the surfactant fully inhibited ATP hydrolysis activity and induced conformational changes in the protein. This interaction made the protein more unstable and denatured at lower temperatures, as observed through enzyme activity and DSC studies.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
