期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 113, 期 4, 页码 442-444出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2011.11.019
关键词
RBS; Bifidobacterium; Shine-Dalgarno sequence; Translation
资金
- Ministry of Education, Culture, Sports, Science and Technology in Japan [20510189]
- C19 Kiyomi Yoshizaki Reserch Grant
- Grants-in-Aid for Scientific Research [20510189] Funding Source: KAKEN
The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.
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