期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 113, 期 6, 页码 694-696出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2012.01.010
关键词
Burst sequence; Very late transcriptional factor-1; Polyhedrin promoter; Baculovirus; Silkworm
资金
- Grants-in-Aid for Scientific Research [22248009, 22780101, 24780111] Funding Source: KAKEN
Modified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat alpha 2,6-sialyltransferase (ST6Gal I) in silkworm. Modified Ppolh of five BSs with VLF-1 coexpression yielded 2.9 U/m1 ST6Gal I activity and 32.5 mU/mg specific activity, which was 1.7- and 2.3-fold higher, respectively compared to Ppolh. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.
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