Article
Engineering, Environmental
Shu-Ting Zhang, Tao Li, Shi-Kai Deng, Jim C. Spain, Ning-Yi Zhou
Summary: This study reveals a novel bacterial metabolic pathway for the degradation of 4-nitroanisole, initiated by a three-component cytochrome P450 system (pnaABC). This system exhibits broad substrate specificity and can catalyze the degradation of nitroanisole derivatives.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yanhui Lu, Qi Bai, Qiang Li, Xusong Zheng, Junce Tian, Jiawen Guo, Hongxing Xu, Zhongxian Lu
Summary: The study identified and characterized two cytochrome P450 monooxygenase genes, CsCYP6SN3 and CsCYP306A1, associated with the lethal effect of vetiver against C. suppressalis larvae. Inhibition of these genes significantly reduced pupation rate and pupa weight in the pests.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Gustavo Perez Ortiz, John D. Sidda, Emmanuel L. C. de los Santos, Catherine B. Hubert, Sarah M. Barry
Summary: The antimycobacterial peptides rufomycins exhibit antibiotic activity due to oxidative tailoring of the cyclic peptide. Cytochrome P450s RufS and RufM play roles in regioselective epoxidation and alkyl oxidation, creating a complex product profile dependent on redox partner availability. Additionally, in vitro one pot conversion of rufomycin B to rufomycin C has been successfully demonstrated.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Biochemistry & Molecular Biology
Jiawei Liu, Hongmin Hu, Huimin Shen, Qingyin Tian, Wenjie Ding, Xiulian Yang, Lianggui Wang, Yuanzheng Yue
Summary: This study identified a total of 276 P450 genes belonging to 40 families in Osmanthus fragrans, suggesting their potential roles in fragrance synthesis. The research provides valuable insights into the functions of P450 genes and serves as a guide for further studies on promoting fragrance biosynthesis in ornamental plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hongsheng Gao, Huiqing Huang, Kaifeng Lu, Cuiting Wang, Xiaohua Liu, Zhizhong Song, Houjun Zhou, Lei Yang, Bei Li, Chunyan Yu, Hongxia Zhang
Summary: The expression of OsCYP714D1 gene improved salt tolerance in transgenic poplar plants by regulating gibberellin and ion homeostasis, resulting in increased plant height, K+ content, and decreased growth inhibition and Na+ content under high salt stress conditions. Transcriptomic analyses revealed up-regulation of GA biosynthesis, signaling, and stress responsive genes in transgenic plants, while gene ontology analyses indicated enrichment of genes involved in plant hormone and ion metabolic activities in the transgenic plants. OsCYP714D1 can be used as a candidate gene for engineering new tree varieties with improved biomass production and salt stress resistance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Pakjira Nanudorn, Sirinthra Thiengmag, Friederike Biermann, Pelin Erkoc, Sabrina D. Dirnberger, Thao N. Phan, Robert Fuerst, Reiko Ueoka, Eric J. N. Helfrich
Summary: This study discovered a new family of ribosomally synthesized and posttranslationally modified peptides, named atropopeptides. The characterization of two atropopeptide biosynthetic pathways revealed a two-step maturation process. Atropopeptides promote pro-angiogenic cell functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Gabriela C. Schroeder, Martha S. Smit, Diederik J. Opperman
Summary: CYPs/P450s are versatile hemethiolate proteins with high stereo- and regio-selectivity, making them powerful biocatalysts for synthesizing value-added compounds. This review highlights recent advances in P450-mediated syntheses, including enzyme engineering strategies to enhance selectivity and facilitate bond formation, as well as approaches to overcome limitations of P450 catalysis for improved efficiency and biotechnological viability.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Carlos A. G. Rigon, Luan Cutti, Guilherme M. Turra, Enrico Z. Ferreira, Christian Menegaz, Walker Schaidhauer, Franck E. Dayan, Todd A. Gaines, Aldo Merotto
Summary: Herbicide mixtures are commonly used to control a wide range of weeds, including those resistant to specific herbicides. However, the impact of mixtures on the evolution of herbicide resistance through enhanced metabolism is not well understood. In this study, the effects of a fenoxaprop-p-ethyl and imazethapyr mixture on herbicide resistance evolution in Echinochloa crus-galli were evaluated. Results showed that recurrent selection with the mixture led to decreased control and increased resistance to several herbicides. Additionally, it was found that the main contributor to decreased control was fenoxaprop. This study highlights the need for using fully recommended herbicide rates in mixtures to reduce the risk of resistance evolution.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Jing Yang, Buli Fu, Peipan Gong, Chengjia Zhang, Xuegao Wei, Cheng Yin, Mingjiao Huang, Chao He, Tianhua Du, Jinjin Liang, Shaonan Liu, Yao Ji, Hu Xue, Chao Wang, Jinyu Hu, He Du, Rong Zhang, Xin Yang, Youjun Zhang
Summary: In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that the mRNA levels of these two genes were up-regulated after exposure to thiamethoxam. Transcriptional levels were overexpressed in resistant strains and positively correlated with resistance levels. Silencing these genes increased whitefly susceptibility to thiamethoxam. These findings provide insights into the role of cytochrome P450 monooxygenases in neonicotinoid resistance and suggest their potential as target genes for pest management.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kai Tian, Jing Feng, Jiang Zhu, Jiagao Cheng, Mei Li, Xinghui Qiu
Summary: The study found that three paralogous CYP9As were capable of metabolizing multiple pyrethroids, with a greater efficiency in transforming pyrethrin-resembling pyrethroids. These results support the hypothesis that herbivorous insects can cope with synthetic insecticides through their physiological systems.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Review
Plant Sciences
Takatoshi Wakabayashi, Kotomi Ueno, Yukihiro Sugimoto
Summary: This article reviews the history leading to the discovery of the genuine structure of orobanchol and the current understanding of its biosynthetic mechanisms.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Martha Sophia Smit, Mpeyake Jacob Maseme, Jacqueline van Marwijk, Jasmin Cara Aschenbrenner, Diederik Johannes Opperman
Summary: Cytochrome P450 monooxygenases (CYP450s) are crucial in the synthesis and breakdown of secondary metabolites in eukaryotes, especially in plants and fungi. CYP505E3 from Aspergillus terreus demonstrates remarkable omega-7 hydroxylase activity, making it a promising catalyst for delta-dodecalactone synthesis. Other CYP505Es also display similar activity, but their occurrence is limited to certain species like Aspergillus and Penicillium.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Jiankang Xin, Tianmin Che, Xiaolong Huang, Huiqing Yan, Shan Jiang
Summary: In this study, the functions of PpCYP98 in Physcomitrium patens were investigated using transcriptome and metabolome analyses. A total of 5266 differentially expressed genes (DEGs) and 68 differentially abundant secondary metabolites were identified between wild-type and ∆PpCYP98 gametophores. The results showed that PpCYP98 plays vital roles in the pre-lignin synthesis pathway and the synthesis of flavonoids in bryophytes. PpCYP98 also showed systemic effects on metabolisms, including carbohydrate, fatty acid, and hormonal signaling transductions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
