期刊
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
卷 108, 期 5, 页码 425-428出版社
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2009.05.009
关键词
Immobilization of liposome; NBD-cholesterol; alpha-Chymotrypsin; Stimuli responsive copolymer; Fluorescence microscope
资金
- Ministry of Education, Science, Sports, and Culture of Japan [19656203, 19656220, 20360350, 20760539, 21246121]
- JSPS
- Global COE Program
- Grants-in-Aid for Scientific Research [20760539, 19656203] Funding Source: KAKEN
NBD-cholesterol (NBD-Ch)-modified liposome was immobilized on indium tin oxide (ITO) electrode via the covalent binding method. The transfer of NBD-Ch between the immobilized liposomes and the target liposomes was observed by using a fluorescent microscope. The addition of liposome suspension co-incubated with alpha-chymotrypsin or stimuli-responsive polymer to the surface of the above ITO electrode, enhanced the liposome-liposome interaction, resulting in the promotion of NBD-Ch transfer. The apparent transfer rate constant of NBD-Ch was found to be correlated with the index for the liposome-liposome interaction evaluated by an immobilized liposome chromatography. This suggests that the present method using the liposome-immobilized ITO electrode was effective to evaluate the liposome-liposome interaction induced by the protein or the stimuli-responsive polymer under stress conditions. (C) 2009, The Society for Biotechnology, Japan. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据