Article
Energy & Fuels
Anu Sadasivan Nair, Nallusamy Sivakumar
Summary: The utilization of waste paper for biodiesel production has environmental and economic advantages. In this study, waste paper hydrolysate (WPH) was used as a substrate for microbial lipid production. The medium with NH(4)C1 as nitrogen source yielded high biomass and lipid content. Under optimized conditions, the maximum biomass, lipid yield, and lipid content obtained were 6.51 g/L, 2.8 g/L, and 43% respectively. Extracted lipids showed similarities to vegetable oils, and the biodiesel properties were within the permissible limits of international standards.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Microbiology
Wei Xiang, Ye Liang, Shan Hong, Guan Wang, Jing You, Yanfen Xue, Yanhe Ma
Summary: A novel bacterial strain, CH91, isolated from a high-temperature oil reservoir, showed the potential for degrading a broad range of long-chain n-alkanes and had high thermal tolerance. It could have applications in bioremediation of oil contamination and microbial enhanced oil recovery.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Laura Salusjarvi, Leo Ojala, Gopal Peddinti, Michael Lienemann, Paula Jouhten, Juha-Pekka Pitkanen, Mervi Toivari
Summary: This study engineered the bacterium R. opacus DSM 43205 to synthesize polymer precursors from CO2. The expression of specific enzymes resulted in the production of beta-alanine or L-lactic acid. Heterotrophic cultivation on glucose showed higher yields compared to autotrophic cultivation with CO2, H-2, and O-2. Additionally, electrobioreactors were used to produce beta-alanine from CO2 through water electrolysis. These findings demonstrate the potential of R. opacus DSM 43205 for metabolic engineering and chemical production from CO2.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Soon Dong Lee, In Seop Kim
Summary: These two novel actinobacterial strains isolated from soil samples of a cave in Jeju Island, Republic of Korea, were classified as Rhodococcus spelaei sp. nov. based on a combination of morphological, physiological, and genomic analyses. Furthermore, genome analysis revealed the heterotypic synonyms relationships within the genus Rhodococcus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Carmen M. Sanchez-Arevalo, Maria Cinta Vincent-Vela, Maria-Jose Lujan-Facundo, Silvia Alvarez-Blanco
Summary: Among all membrane processes, ultrafiltration is widely used for both aqueous filtrations and solvent-based solutions. However, the challenges of membrane contact with organic solvents, such as permeate flux, rejection values, and long-term stability, remain. This review examines the effects of solvents on the ultrafiltration process and focuses on membrane pre-treatment strategies. It provides valuable knowledge and insights into organic solvent ultrafiltration.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2023)
Article
Plant Sciences
Toktam Taghavi, Hiral Patel, Reza Rafie
Summary: Anthocyanins, major plant pigments with antioxidant activities, are influenced by various factors in the extraction process. This study compared different solvents and their combinations to extract anthocyanins from strawberries. The results showed that methanol and methanol:water were the best solvents for anthocyanin assessment, while acetone did not produce a stable extract. The choice of extraction solvent also affected the profile of anthocyanins, with different solvents yielding different major compounds.
Article
Engineering, Chemical
Yunqiao Ma, Sadiye Velioglu, Ziqiang Yin, Rong Wang, Jia Wei Chew
Summary: This study uses molecular dynamics simulations to explore the different adsorption behaviors of dextran onto a polyacrylonitrile membrane in three polar and protic solvents: water, formamide, and ethanol. The results show that the dextran-membrane separation distance is the shortest in water, leading to the highest adsorption tendency. This research highlights the need for a better understanding of membrane fouling behaviors in organic solvents.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Engineering, Chemical
Eric Ziemann, Arindam Kumar Das, Paramita Manna, Revital Sharon-Gojman, Michal Sela-Adler, Charles Linder, Roni Kasher, Roy Bernstein
Summary: This study presents a facile method to fabricate crosslinked polyethersulfone membranes with remarkable stability in organic solvents. The performance of the membranes can be easily adjusted by controlling the polymer fraction. The weeklong filtration experiment highlights the excellent stability of the membranes.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Engineering, Chemical
Siyan Wang, Zhenggong Wang, Shouwen Zhu, Shuqi Liu, Feng Zhang, Jian Jin
Summary: Membranes with high stability and selectivity are needed for efficient chemical separations in harsh solvents. In this study, a thin-film composite polyamide membrane for organic solvent nanofiltration (OSN) was fabricated using an adamantane diamine as a building block. The bulky adamantane structure inhibited polymer chain packing, creating interconnected micropores in the polyamide layer. The membrane exhibited high methanol permeance and retention of organic dye molecules, as well as stability in different organic solvents. This approach improves membrane permeability and solvent resistance without compromising selectivity.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Multidisciplinary Sciences
Yanqiu Lu, Liling Zhang, Liang Shen, Wei Liu, Rohit Karnik, Sui Zhang
Summary: Based on molecular dynamics simulation, a resistance model was established to guide the design of defect-tolerant graphene composite membranes for better control of solvent permeation. Experimental results showed that improved supporting substrates could effectively mitigate leakage through defects in graphene, enabling selective transport of specific molecules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Materials Science, Paper & Wood
Do-Hyun Kim, Seungyeol You, Myeung-jin Lee, Hong-Dae Kim, Gyu Tae Kim
Summary: This study investigated the dynamics of absorption and evaporation of various organic solvents in wood by monitoring changes in electric current. It was found that walnut wood exhibited different degrees of absorption and evaporation for polar and nonpolar solvents, with acetone and n-hexane being absorbed and evaporated most rapidly in electrically conductive wood.
Article
Biochemistry & Molecular Biology
Chen Wu, Yumeng Yang, Kaiqi Sun, Dan Luo, Xiuyu Liu, Huining Xiao, Huiyang Bian, Hongqi Dai
Summary: Organic solvent fractionation was used to improve the UV absorption performance and color of industrial lignin for better utilization as a UV shield. The physicochemical properties of lignin and lignin-based sunscreens were analyzed and compared, and the soluble fraction showed significantly better color and UV-shielding properties than the original and insoluble fractions. The composite sunscreen with 5 wt% acetone fractionated lignin exhibited the highest sun protection factor (SPF) value of 6.6, approximately 4.5 times higher than sunscreens with pristine lignin. This study highlights the potential of industrial lignin in value-added applications such as UV protection and cosmetics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Nobuyuki Onishi, Mie Minagawa, Akihiko Tanioka, Hidetoshi Matsumoto
Summary: This study measured and compared the chronopotentiometric responses, pH changes, and current-voltage characteristics of BPM/LiCl-organic solvent systems and BPM/LiCl-water system, and revealed the influence of organic solvents on the dissociation behavior of electrolytes. It was found that amides differ from alcohols in their dissociation behavior at the BPM interface.
Article
Biochemistry & Molecular Biology
Yuan Yuan, Fan Zhang, Lei Ai, Yuzhu Huang, Ren Peng
Summary: In this study, a novel c-di-GMP binding protein named CEP1 was identified and its interaction with c-di-GMP was confirmed using surface plasmon resonance. The mRNA levels of the cep1 gene in Rhodococcus ruber SD3 were significantly increased under toluene and phenol stress. A cep1 gene-enhanced strain was constructed, which exhibited improved growth in the presence of different organic solvents. This study provides insights into the mechanism linking c-di-GMP and organic solvent tolerance in Rhodococcus ruber SD3.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Environmental Sciences
Zeyu Wang, Liyong Hu, Min Zhao, Luyao Dai, Dzmitry Hrynsphan, Savitskaya Tatsiana, Jun Chen
Summary: This study successfully developed a bamboo charcoal-modified polyurethane foam that effectively removes organic solvents from oily wastewater. The addition of bamboo charcoal improved the mechanical properties of polyurethane, and the maximum adsorption capacity of the BC/PU foam was 23.6 g g(-1). The adsorption mechanism of organic solvents on BC/PU-5 was mainly attributed to electron donor-acceptor interaction and non-hydrophobic interaction, as supported by density functional theory simulation. Additionally, the adsorption capacity remained stable after multiple recycling cycles. The prepared BC/PU foam composites show great potential as candidates for separating organic solvents in engineering applications.
Article
Biotechnology & Applied Microbiology
Norichika Ogata, Akio Nishimura, Tomoko Matsuda, Michi Kubota, Takeshi Omasa
Summary: Cell-to-cell variability arises from a combination of intrinsic factors and extrinsic factors. Heterogeneity in high cell density suspension cultures for therapeutic protein production remains unknown. This study illustrates increasing heterogeneity in cellular transcriptome during high cell density suspension culture, without concomitant changes in genomic sequence.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Masahide Kido, Hideaki Idogaki, Kouji Nishikawa, Takeshi Omasa
Summary: This study evaluated the potency of violacein for cell cycle control and improvement of IgG production in CHO cells. Results showed that violacein increased IgG production by inducing G2/M phase arrest and without affecting product quality. This suggests that violacein has potential as a mAb production enhancer in CHO cells.
Article
Biotechnology & Applied Microbiology
Gladwin Suryatin Alim, Tomoka Iwatani, Kenji Okano, Shigeru Kitani, Kohsuke Honda
Summary: Coenzyme A (CoA) is a vital cofactor involved in various metabolic pathways, but its accumulation in cells is challenging. In vitro production of CoA using recombinant enzymes has been explored as a solution to this issue.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kenji Okano, Yu Sato, Tatsuya Hizume, Kohsuke Honda
Summary: The CRISPR/Un1Cas12f1 system is an efficient genome editing tool that shows higher efficiency in editing bacterial genes compared to the commonly used CRISPR/SpCas9 system, and is expected to be widely applied in bacterial genome editing.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Chih-Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama
Summary: The overexpression of RtFAD2 in oleaginous yeast Rhodotorula toruloides significantly increased the production of linoleic acid (LA), indicating a potential application in the production of LA or polyunsaturated fatty acids. This finding may assist in the rational design of metabolic engineering related to LA production in R. toruloides.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Hajime Enatsu, Nako Okamoto, Yoshiki Nomura, Masayoshi Onitsuka, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa
Summary: Cartilaginous fishes like sharks have adaptive immune systems based on immunoglobulins, known as Immunoglobulin new antigen receptors (IgNARs). A recombinant expression system for IgNAR was successfully constructed in CHO cells, leading to successful expression and purification of IgNAR with antigen-binding ability.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Cell Biology
Andrew Samy, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa
Summary: GRP94 is a chaperone protein involved in folding of unfolded proteins in the endoplasmic reticulum, and the truncated form without the KDEL motif can be naturally secreted into the medium in response to ER stress. This secretion phenomenon may shed light on the functional role of GRP94 as a trafficking molecule during the unfolded protein response.
Article
Biotechnology & Applied Microbiology
Makoto Imura, Shinichi Etoh, Ryo Iwakiri, Kenji Okano, Kohsuke Honda
Summary: The demand for L-cysteine is increasing, with traditional production methods causing environmental issues and market limitations. Recent studies have focused on microbial fermentation alternatives but faced yield challenges. This study successfully redesigned an in vitro synthetic pathway to produce high yields of L-cysteine from glucose.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Gladwin Suryatin Alim, Kenji Okano, Kohsuke Honda
Summary: This study addressed the issue of using too many enzymes in the ATP regeneration system by constructing co-expression vectors to achieve simultaneous gene expression of the 12 enzymes in a single strain. It also provided a strategy to rationally design and control protein expression levels in the co-expression vectors.
Article
Biotechnology & Applied Microbiology
Yutaka Kirimoto, Noriko Yamano-Adachi, Yuichi Koga, Takeshi Omasa
Summary: Therapeutic antibodies are highly effective biopharmaceuticals with fewer side effects and longer half-life in the blood. This study focused on the intracellular trafficking of antibody-producing CHO cells and identified the accumulation of antibodies in the endoplasmic reticulum (ER) due to insufficient cargo receptors. Overexpression of the cargo receptor complex, ERGIC-53-MCFD2, improved antibody production but also increased intracellular antibody retention.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Noriko Yamano-Adachi, Yuto Nakanishi, Wataru Tanaka, YuanShan Lai, Masahiro Yamazaki, Laura Zenner, Hirofumi Hata, Takeshi Omasa
Summary: This study investigated the effects of artificial changes in the chromosome number on productivity in CHO cells. It was found that cell populations with a diverse chromosome number distribution exhibited higher antibody productivities. Additionally, inducing aneuploid cells using 3-aminobenzamide increased antibody productivity. Furthermore, using host cells with an average chromosome number of 37 significantly improved antibody productivity and increased the integration of exogenous genes into the genome.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Shunsuke Kato, Koki Takeuchi, Motonao Iwaki, Kentaro Miyazaki, Kohsuke Honda, Takashi Hayashi
Summary: Affinity purification of recombinant proteins is an essential technique in biotechnology. However, current methods are costly, limiting their versatile use. To address this issue, we developed the CSAP system, using commercially available chitin powder as a chromatography matrix for low-cost purification of Strep-tag II fusion proteins. We demonstrated the system's effectiveness in protein screening using 96-well format, identifying potential candidates for an abiotic carbene transfer reaction.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Correction
Microbiology
Hironori Taniguchi, Sathidaphorn Sungwallek, Phatcharin Chotchuang, Kenji Okano, Kohsuke Honda
JOURNAL OF BACTERIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Zhou Xing, Thao Bich Nguyen, Guirong Kanai-Bai, Noriko Yamano-Adachi, Takeshi Omasa
Summary: A novel kinetic model was developed to simulate the production process of a CVA6 VLP vaccine, and the optimal operating conditions that balanced product yield and quality were predicted through multiple objective optimization.
Article
Biochemistry & Molecular Biology
Mohammad Mojarrad, Takahisa Tajima, Akiko Hida, Junichi Kato
Summary: This study evaluated the simultaneous biosynthesis of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO) using a new psychrophile-based simple biocatalyst (PSCat) reaction system. The maximum production of 3-HP was 62.0 mm in solo production and 25.0 mm for 1,3-PDO. Through the PSCat approach, productivity was boosted with a 13.5 mm increase in 3-HP and 4.9 mm increase in 1,3-PDO in the simultaneous production process.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)