Review
Biotechnology & Applied Microbiology
Paloma Liras, Juan F. Martin
Summary: Transcriptomic, proteomic, and metabolomic studies have enhanced our understanding of the biosynthesis and regulation of clavuligerus, leading to the discovery of new metabolites. The role of specific activators and regulators in gene expression has been elucidated, providing insights for improving industrial applications and clavulanic acid production.
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Arshad Ali Shaikh, Louis-Felix Nothias, Santosh K. Srivastava, Pieter C. Dorrestein, Kapil Tahlan
Summary: This study utilized an overexpression strategy in combination with untargeted metabolomics, molecular networking, and in silico analysis to annotate 28 metabolites not previously reported in Streptomyces clavuligerus. The manipulation of frr and rpsL in specific conditions led to the production of commonly found plant compounds in S. clavuligerus, with different metabolite production profiles observed. Known and putative gene clusters associated with the production of observed compounds were discussed, highlighting the potential of combining traditional strain engineering and metabolomics technologies for the rapid discovery of novel natural products.
Article
Biotechnology & Applied Microbiology
Aslihan Kurt-Kizildogan, Gozde Celik, Eser unsaldi, Servet Ozcan, Serife Ayaz-Guner, Gulay Ozcengiz
Summary: This study deciphers regulatory mechanisms in clavulanic acid biosynthesis and provides insights for enhancing CA yields through analyzing transcriptome and proteome alterations in an industrial overproducer strain.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Max M. Maurer, Marius Ibach, Julius Plewe, Axel Winter, Paul Ritschl, Brigitta Globke, Robert oellinger, Georg Lurje, Wenzel Schoening, Johann Pratschke, Dennis Eurich
Summary: The objective of this study was to investigate the adherence, efficacy, and safety after converting stable liver transplant patients from IR-Tac to LCP-Tac. The results showed that LCP-Tac improved adherence and was safe for use in liver transplant patients.
Article
Biotechnology & Applied Microbiology
Luisa F. Patino, Vanessa Aguirre-Hoyos, Laura I. Pinilla, Leon F. Toro, Rigoberto Rios-Estepa
Summary: This study found that the overexpression of the orf21 gene can regulate the production of clavulanic acid in Streptomyces, and is influenced by the nutritional composition of the culture medium.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Petra Pivk Lukancic, Tjasa Drcar, Robert Bruccoleri, Martin Crnugelj, Peter Mrak
Summary: In this study, a new genotype of Streptomyces clavuligerus with the ability to produce teleocidin was discovered. Teleocidin is a highly toxic secondary metabolite. The genome sequence of this genotype provides a foundation for further research and application.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Soonkyu Hwang, Namil Lee, Donghui Choe, Yongjae Lee, Woori Kim, Yujin Jeong, Suhyung Cho, Bernhard O. Palsson, Byung-Kwan Cho
Summary: In this study, transcriptional regulatory elements in Streptomyces clavuligerus were identified using genome-wide transcript 3'-end positions and transcription units. The study revealed the importance of transcript 3'-end positions in controlling transcription levels and the potential for designing engineering tools to enhance secondary metabolite production based on these findings.
Article
Genetics & Heredity
Buesra Abanoz-Secgin, Cigdem Otur, Sezer Okay, Aslihan Kurt-Kizildogan
Summary: The SCLAV_3199 gene plays a significant role in regulating iron homeostasis and secondary metabolite biosynthesis in Streptomyces clavuligerus.
Article
Microbiology
Chaobo Zhang, Youbao Zhao, Zilong Li, Weishan Wang, Ying Huang, Guohui Pan, Keqiang Fan
Summary: In this study, it was found that GylR regulates glycerol metabolism in Actinobacteria by directly binding to the promoter regions of glp operon and gylR. Glycerol-3-phosphate and dihydroxyacetone phosphate induce the dissociation of GylR from its binding sequences, and a minimal essential operator site for GylR-like regulators in Streptomyces was identified.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Victor A. Lopez-Agudelo, David Gomez-Rios, Howard Ramirez-Malule
Summary: Clavulanic acid is an irreversible beta-lactamase enzyme inhibitor with weak antibacterial activity, typically combined with antibiotics to treat diseases caused by beta-lactam resistant bacteria. Improvements in large-scale bioprocessing and integration of metabolic engineering strategies with systems biology methods are essential to optimize production and reduce costs associated with clavulanic acid.
Article
Health Care Sciences & Services
Chih-Yang Hsiao, Ming-Chih Ho, Cheng-Maw Ho, Yao-Ming Wu, Po-Huang Lee, Rey-Heng Hu
Summary: The study investigated the impact of tacrolimus blood concentration after five years of liver transplant on long-term patient survival outcomes, with findings suggesting that patients with a tacrolimus trough level of 4.6-10.2 ng/mL had significantly better long-term survival. The recommendation from the study is that adult liver transplant patients should maintain a tacrolimus level of no lower than 4.6 ng/mL for optimal long-term survival.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Ophthalmology
Joshua Luis, Abdulrahman Alsaedi, Sumita Phatak, Bharat Kapoor, Angela Rees, Mark Westcott
Summary: Tacrolimus was found to be effective and well tolerated in treating noninfectious uveitis in this study. Higher trough serum tacrolimus concentrations were associated with better disease control, while high-dose use of oral prednisolone was significantly reduced.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2022)
Article
Medicine, General & Internal
Zhi-yu Zou, Lin-rui Dai, Yi-bo Hou, Chen-zhen Yu, Ren-jie Chen, Yan-yan Chen, Bin Liu, Hui-bo Shi, Nian-qiao Gong, Zhi-shui Chen, Song Chen, Sheng Chang, Wei-jie Zhang
Summary: This study found that an immunosuppressive regimen of SRL combined with low-dose ER-TAC is equally effective and safe as standard immunosuppressive regimens for renal transplant recipients, and may improve graft renal function and medication adherence.
FRONTIERS IN MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Maan Abdullah Albarry, Mohit Parekh, Stefano Ferrari, Heba Mahmoud Eltahir, Ahmed M. Shehata, Mohamed A. Shaker, Hossein Mostafa Elbadawy
Summary: This study assessed the effect of different concentrations of tacrolimus eye suspension on human corneal cells and investigated its safety for severe cases of vernal keratoconjunctivitis. The results showed that 0.1% concentration of tacrolimus had the best viability of corneal tissue, and incremental concentrations significantly improved VKC cases.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Agricultural Engineering
Vinod Kumar, Anu Radha, Varsha Sharma, Nagaraju Nekkala, Saurabh Saran
Summary: The aim of this study was to investigate the use of xylose enriched spent lemongrass hydrolysate as an alternative production medium for the production of clavulanic acid by Streptomyces clavuligerus. Xylose was extracted from spent lemongrass using dilute nitric acid and further purified using ion exchange resin. The optimal conditions for cell growth and clavulanic acid production were determined, resulting in an effective production of 0.59 g/L clavulanic acid.
BIORESOURCE TECHNOLOGY
(2023)