期刊
JOURNAL OF BIOPHOTONICS
卷 3, 期 8-9, 页码 588-596出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201000023
关键词
FT-IR; IR-MSP; point mode; imaging mode; spectral cytopathology; oral mucosa; principal component analysis
资金
- NCI NIH HHS [R21 CA153148, CA 090346, R01 CA090346, R01 CA090346-01A2] Funding Source: Medline
In this paper we describe the advantages of collecting infrared microspectral data in imaging mode opposed to point mode. Imaging data are processed using the PapMap algorithm, which co-adds pixel spectra that have been scrutinized for R-Mie scattering effects as well as other constraints. The signal-to-noise quality of PapMap spectra will be compared to point spectra for oral mucosa cells deposited onto low-e slides. Also the effects of software atmospheric correction will be discussed Combined with the PapMap algorithm, data collection in imaging mode proves to be a superior method for spectral cytopathology. [GRAPHICS] Visible image of oral mucosa cells deposited onto low-e microscope slides, infrared intensity image, and binary mask image produced in PapMap (left to right).
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