Article
Biochemistry & Molecular Biology
Mengying Wang, Plamen Todorov, Wanxue Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Vladimir Isachenko
Summary: This study found that freezing spermatozoa leads to more down-regulation of genes, which is related to apoptosis changes and immune response. Vitrification induces minor biological changes in human spermatozoa compared to conventional freezing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Tie Chang, Oyawale Adetunji Moses, Conghui Tian, Hai Wang, Li Song, Gang Zhao
Summary: In this study, tungsten diselenide (WSe2)-polyvinyl pyrrolidone (PVP) nanoparticles (NPs) were synthesized and shown to exhibit synergistic ice regulation ability. These NPs were successfully used for cryopreservation of human umbilical vein endothelial cell (HUVEC)-laden constructs, resulting in increased cell viabilities and good condition maintenance in vivo within 7 days. This work provides a novel strategy to suppress ice crystal formation and growth for the cryopreservation of cells, tissues, or organs.
Article
Agriculture, Dairy & Animal Science
Maria Gemma Millan de la Blanca, Eva Martinez-Nevado, Cristina Castano, Juncal Garcia, Berenice Bernal, Adolfo Toledano-Diaz, Milagros Cristina Esteso, Paula Boveda, Lucia Martinez-Fresneda, Antonio Lopez-Sebastian, Julian Santiago-Moreno
Summary: The American flamingo is a useful model for developing successful semen cryopreservation procedures, applicable to threatened related species from the family Phoenicopteridae, to allow genetic material banking. The study aimed to develop effective sperm cryopreservation protocols by examining the influences of two permeating cryoprotectants and seminal plasma removal. The findings showed that there were no differences between Me2SO and DMA for successful freezing of flamingo sperm, and seminal plasma removal did not benefit sperm cryopreservation.
Article
Cell Biology
Aisha Naeem, Nikita Gupta, Natasha Arzoo, Usra Naeem, Muhammad Jawad Khan, Muhammad Umer Choudhry, Wanxing Cui, Chris Albanese
Summary: This article critically evaluates the methods used for isolation, expansion, and cryopreservation of human amniotic epithelial cells (hAECs) and discusses the progress and challenges in optimizing clinically suitable culture conditions for ex-vivo expansion and storage of these cells. The review emphasizes the importance of overcoming limitations in maintaining the stemness of hAECs in both xenogeneic and xenofree culture conditions.
Article
Obstetrics & Gynecology
Yodo Sugishita, Lingbo Meng, Yuki Suzuki-Takahashi, Sandy Nishimura, Sayako Furuyama, Atsushi Uekawa, Akiko Tozawa-Ono, Junko Migitaka-Igarashi, Tomoe Koizumi, Hibiki Seino, Yasunori Natsuki, Manabu Kubota, Junki Koike, Keisuke Edashige, Nao Suzuki
Summary: After thawing/warming, at least 60 minutes of diffusion washing in media was necessary to significantly reduce the residual cryoprotectants in ovarian tissues frozen by slow freezing or vitrification.
HUMAN REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Nela Pilbauerova, Jan Schmidt, Tomas Soukup, Romana Koberova Ivancakova, Jakub Suchanek
Summary: This study aimed to observe the effect of uncontrolled-rate freezing on DPSCs stored for 6 and 12 months. It was found that DPSCs retained their stemness markers and low-level expression of hematopoietic markers even after one year of cryopreservation at -80 degrees C, suggesting that DPSCs can still be successfully used in potential clinical applications after a year of uncontrolled cryopreservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yushi Abe, Daigo Ochiai, Masako Taguchi, Seiji Kanzaki, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: Human amniotic fluid stem cells (hAFSCs) can form aggregates with host immune cells and alleviate inflammation. Additionally, hAFSCs can increase regulatory T cells in the acquired immune system. Therefore, hAFSCs play an important role in the treatment of inflammation-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Francesca Casciaro, Silvia Zia, Mattia Forcato, Manuela Zavatti, Francesca Beretti, Emma Bertucci, Andrea Zattoni, Pierluigi Reschiglian, Francesco Alviano, Laura Bonsi, Matilde Yung Follo, Marco Demaria, Barbara Roda, Tullia Maraldi
Summary: This study utilized Celector® technology to classify and characterize hAFSCs, uncovering deep intra-population differences and validating their impact on stemness properties. The results suggest the importance of selecting cellular fractions with the highest potential for use in regenerative medicine.
Review
Biochemistry & Molecular Biology
Ambra Costa, Rodolfo Quarto, Sveva Bollini
Summary: Studies have shown that small extracellular vesicles (EVs) obtained from human amniotic fluid stem cells (hAFSC) and human amniotic fluid (hAF) have regenerative effects and can be used for tissue recovery and treatment of various diseases. However, the isolation, characterization, and quantification methods of EVs remain challenging for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Athanasia Dasargyri, Dayme Gonzalez Rodriguez, Hubert Rehrauer, Ernst Reichmann, Thomas Biedermann, Ueli Moehrlen
Summary: This study characterized the tissue origin and marker expression of cultured amniotic fluid cells at the single-cell level using single-cell RNA sequencing. It revealed the heterogeneity of amniotic fluid cells and identified cell types of neural origin in fetuses with spina bifida aperta. The findings provide valuable information for further research and clinical applications of amniotic fluid cells.
Review
Obstetrics & Gynecology
Marisa Kometas, Gregory M. Christman, Joseph Kramer, Alice Rhoton-Vlasak
Summary: The effectiveness of vitrification compared to slow freezing for preserving ovarian tissue remains controversial, with limited clinical data and inconsistent findings from biochemical studies. While early clinical outcomes suggest favorability towards vitrification, further evaluation is needed to determine its long-term efficacy. Despite potential lack of difference in outcomes between the two methods, the cost-effectiveness and efficiency of vitrification make it a more accessible option.
REPRODUCTIVE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Carolina C. Zuliani, Ingrid I. Damas, Kleber C. Andrade, Cecilia B. Westin, Angela M. Moraes, Ibsen Bellini Coimbra
Summary: The study reveals the potential of amniotic fluid stem cells (AFSCs) combined with scaffolds in reconstructing articular cartilage, showing successful differentiation into chondrocytes. The experiments demonstrated that AFSCs cultured in both a micromass system and directly in a scaffold can differentiate into chondrocytes effectively.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Aiste Zentelyte, Deimante Zukauskaite, Ieva Jaceryte, Veronika V. Borutinskaite, Ruta Navakauskiene
Summary: In this study, short-term treatments of small molecules were used to enhance the properties and differentiation capability of human amniotic fluid stem cells (AFSC). The combinations of epigenetically active compounds and multifunctional molecules upregulated genes involved in pluripotency, leading to a more energetically active phenotype in AFSCs. The small molecule treatments also showed a beneficial effect on differentiation potential towards a neurogenic lineage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Giulia Gaggi, Andrea Di Credico, Simone Guarnieri, Maria Addolorata Mariggio, Angela Di Baldassarre, Barbara Ghinassi
Summary: Human amniotic fluids stem cells (hAFSCs) can differentiate into spinal motor neurons and form functional neuromuscular junctions, providing a promising approach for the study and treatment of neurodegenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Computer Science, Interdisciplinary Applications
Benedikt X. Scholz, Yusuke Hayashi, Isuru A. Udugama, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study presents a multilayered model-based approach for scaling up slow and forced convection-based freezing of human induced pluripotent stem cells. The approach combines a hybrid single-cell freezing model with a computational fluid dynamics model, and takes into account the spatial and timedependent temperature profiles within the freezer unit in calculating the cell survival rate.
COMPUTERS & CHEMICAL ENGINEERING
(2022)
Article
Obstetrics & Gynecology
Natalia S. Carvalho, Antonio F. Moron, Steven S. Witkin, Ramkumar Menon, Sergio Cavalheiro, Mauricio M. Barbosa, Herbene J. Milani, Stephanno G. Sarmento, Marcia M. Ishigai
Summary: This study evaluated the repair process of fetal membranes after open fetal surgery for myelomeningocele. The results showed increased collagen, altered expression of MMP-9 and TIMP-1, and the repair process was also influenced by the number of previous pregnancies and gestational age at the time of surgery.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Tatiane Boute, Giuseppe Rizzo, Ilenia Mappa, Alexander Makatsariya, Bruno Rodrigues Toneto, Antonio Fernandes Moron, Liliam Cristine Rolo
Summary: The objective of this study was to evaluate the correlation between estimated fetal weight (EFW) calculated using different formulae and birth weight, as well as to assess the accuracy of classifying fetuses as small for gestational age (SGA) at birth. The results showed a good correlation between EFW and birth weight for infants with gastroschisis, but no correlation for those with omphalocele. All formulae overestimated the number of SGA cases.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Stephanno G. P. Sarmento, Antonio F. Moron, Larry J. Forney, Alan R. Hatanaka, Francisco H. C. Carvalho, Marcelo S. Franca, Tatiana K. Hamamoto, Rosiane Mattar, Iara M. Linhares, Evelyn Minis, Adriana Sanudo, Ester Sabino, Marilza V. C. Rudge, Steven S. Witkin
Summary: In this study, elevation of TIMP-1 and L-lactic acid levels in vaginal secretions, as well as dominance of L. iners in the vaginal microbiome, are associated with an increased risk of spontaneous preterm birth in primigravid women.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Article
Obstetrics & Gynecology
Marcelo Santucci Franca, Alan Roberto Hatanaka, Jader de Jesus Cruz, Valter Lacerda de Andrade Junior, Tatiana Emy Kawanami Hamamoto, Stephanno G. P. Sarmento, Julio Elito Junior, David Baptista da Silva Pares, Rosiane Mattar, Edward Araujo Junior, Antonio Fernandes Moron
Summary: Research on cervical pessary has shown controversial results, with significant differences in patient follow-up after insertion and the number of insertions per center. Treating singleton pregnancies with short cervix with progesterone and possibly cervical pessary can reduce preterm birth rates significantly based on the resources available.
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
(2022)
Article
Acoustics
Herbene Jose Figuinha Milani, Enoch Quindere de Sa Barreto, Edward Araujo Junior, Sergio Cavalheiro, Mauricio Mendes Barbosa, Antonio Fernandes Moron
Summary: A new technique was standardized to evaluate fetal leg muscle trophism, showing significant differences between normal and fetuses with open lumbosacral spina bifida through measurements of leg area and circumference. The method proved to be highly reproducible, with lower measurements observed in fetuses with SB compared to the control group, and particularly in cases with abnormal lower limb movements.
JOURNAL OF ULTRASOUND IN MEDICINE
(2022)
Article
Clinical Neurology
Sergio Cavalheiro, Marcos Devanir Silva da Costa, Emmanuel Sa, Patricia Alessandra Dastoli, Jardel Nicacio Mendonca, Renato Luis da Silveira Ximenes, Stephanno Gomes Pereira Sarmento, Antonio Fernandes Moron
Summary: Spontaneous third ventriculostomy (STV) is a rare condition that is difficult to diagnose and should be suspected when spontaneous hydrocephalus resolution occurs, especially in early childhood. STV is not equivalent to hydrocephalus resolution.
CHILDS NERVOUS SYSTEM
(2021)
Article
Clinical Neurology
Sergio Cavalheiro, Marcos Devanir Silva da Costa, Mauricio Mendes Barbosa, Patricia Alessandra Dastoli, Jardel Nicacio Mendonca, Daniela Cavalheiro, Antonio Fernandes Moron
Summary: The study found that the onset of hydrocephalus is directly related to myelomeningocele closure in neurosurgery. In patients who underwent surgery after the first 6 h of life, 83% developed hydrocephalus after birth and required ventriculoperitoneal shunt placement in the maternity ward.
CHILDS NERVOUS SYSTEM
(2021)
Article
Pediatrics
Antonio Macedo, Sergio Leite Ottoni, Gilmar Garrone, Antonio Moron, Sergio Cavalheiro, Marcela Leal da Cruz
Summary: The study aimed to determine the incidence of lower urinary tract surgery in patients with myelomeningocele operated in utero, as well as in the subgroup of patients aged 5 years and older. The results showed surgery incidence rates of 10.8% for patients over 5 years old and 12.9% for the high-risk group. This information may be useful to educate parents about the importance of urological treatment.
JOURNAL OF PEDIATRIC UROLOGY
(2021)
Letter
Clinical Neurology
Tereza Cristina Carbonari de Faria, Sergio Cavalheiro, Marcos Devanir Silva da Costa, Patricia Alessandra Dastoli, Silvia Lanziotti Azevedo Silva, Jardel Mendonc Nicacio, Italo Caprano Suriano, Mauricio Mendes Barbosa, Antonio Fernandes Moron
WORLD NEUROSURGERY
(2021)
Article
Multidisciplinary Sciences
Tania Regina Tozetto-Mendoza, Maria C. Mendes-Correa, Antonio F. Moron, Larry J. Forney, Iara M. Linhares, Almir Ribeiro da Silva, Layla Honorato, Steven S. Witkin
Summary: The titer of Torquetenovirus (TTV) in the vagina of pregnant women is associated with the composition of vaginal microbiota and the levels of compounds in vaginal secretions. This association suggests that changes in immune status may influence vaginal fluid composition.
Review
Cell Biology
Fabio Alessandro de Freitas, Debora Levy, Cadiele Oliana Reichert, Edecio Cunha-Neto, Jorge Kalil, Sergio Paulo Bydlowski
Summary: Oxysterols have diverse effects on the immune system and play a significant role in immune-related diseases. They can influence the function and migration of immune cells as well as immune responses. The main oxysterol receptors LXR and EBI2 also have important roles in the immune system.
Article
Clinical Neurology
Alexandre Kim Sangalan Sasaoka, Antonio Fernandes Moron, Edward Araujo, Adriana Sanudo, Mauricio Mendes Barbosa, Herbene Jose Figuinha Milani, Stephanno Gomes Pereira Sarmento, Sergio Cavalheiro
Summary: This study aimed to analyze the evolution of uterine scar thickness after open fetal surgery for myelomeningocele and establish a cut-off point for uterine scar thickness associated with high risk of uterine rupture. The results showed that thinner uterine scars were significantly associated with increased likelihood of thinning during pregnancy follow-up. A cut-off point of ≤3.0 mm in scar thickness may be useful for decision-making regarding the risk of uterine rupture and indication of caesarean section.
CHILDS NERVOUS SYSTEM
(2023)
Article
Acoustics
Herbene Jose Figuinha Milani, Enoch Quindere de Sa Barreto, Edward Araujo Junior, Sergio Cavalheiro, Mauricio Mendes Barbosa, Alberto Borges Peixoto, Antonio Fernandes Moron
Summary: This study assessed the trophism of the lower limbs of fetuses with open spina bifida using fractional thigh volume (TVOL) measured by three-dimensional ultrasound. The results showed no significant differences in TVOL between normal fetuses and those with spina bifida, while fetuses with spina bifida and without spontaneous movement of the lower limbs had lower TVOL values.
JOURNAL OF CLINICAL ULTRASOUND
(2023)
Article
Pediatrics
Joao Victor Jacomele Caldas, Antonio Fernandes Moron, David Baptista da Silva Pares, Sergio Cavalheiro, Italo Capraro Suriano, Edward Araujo Junior
Summary: Occipital encephalocele is a rare neural tube defect that requires surgical repair after birth. Intrauterine surgery aims to minimize brain herniation and improve perinatal outcomes. This study presents the first experience of fetal open surgery for occipital encephalocele in Brazil, highlighting the importance of postnatal follow-up to evaluate the benefits.
JOURNAL OF PEDIATRIC NEUROLOGY
(2023)
Article
Clinical Neurology
Marcos Devanir Silva da Costa, Jardel Mendonca Nicacio, Patricia Alessandra Dastoli, Italo Capraro Suriano, Stephanno Gomes Pereira Sarmento, Mauricio Mendes Barbosa, Antonio Fernandes Moron, Sergio Cavalheiro
Summary: Recent studies have shown that fetal surgery can significantly reduce the risk of developing hydrocephalus in individuals with myelomeningocele. This study aimed to develop a training model for the fetal myelomeningocele repair technique using multi-portal endoscopy.
CHILDS NERVOUS SYSTEM
(2023)
