Article
Chemistry, Multidisciplinary
Ziyao Wang, Ziyan Feng, Fangxue Du, Xi Xiang, Xinyi Tang, Li Qiu, Zhiyong Qian
Summary: Ultrasonography is an important complement to clinical diagnosis, and the application of microbubbles effectively improved diagnostic accuracy in echography. In scientific research, the sizes of microbubbles range from nanometers to microns. By optimizing the fabrication process, bubble sizes and ultrasound parameters, microbubbles can also be used for drug delivery and therapeutic monitoring.
CHINESE CHEMICAL LETTERS
(2023)
Article
Pharmacology & Pharmacy
Chia-Wei Lin, Ching-Hsiang Fan, Chih-Kuang Yeh
Summary: This study explores how loading the drug doxorubicin onto microbubbles affects their acoustic properties, finding that the drug loading capacity is determined by the proportion of certain lipid materials with distinct electrostatic properties. Loading doxorubicin onto microbubbles enhances acoustic stability, decreases resonance frequency, and reduces stable cavitation dose, demonstrating a significant impact on the elasticity and fluidity of the microbubble shell.
Article
Cardiac & Cardiovascular Systems
Matthew A. Muller, Todd Belcik, James Hodovan, Koya Ozawa, Eran Brown, Jeffry Powers, Paul S. Sheeran, Jonathan R. Lindner
Summary: The study investigated the impact of cavitation on tissue perfusion using two-dimensional (2D) versus three-dimensional (3D) therapeutic ultrasound, finding that both methods led to a similar degree of muscle flow augmentation. It was discovered that cavitation can produce conducted effects beyond the ultrasound field, resulting in only a modest increase in the spatial extent of flow augmentation.
JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY
(2021)
Article
Nanoscience & Nanotechnology
Lei Dong, Nana Li, Xixi Wei, Yongling Wang, Liansheng Chang, Hongwei Wu, Liujiang Song, Kang Guo, Yuqiao Chang, Yaling Yin, Min Pan, Yuanyuan Shen, Feng Wang
Summary: A new drug-loaded microbubble complex based on PLGA was developed to target GA to brain tumors through UTMD. The combination of GA/PLGA-CMB with FUS demonstrated a significant inhibitory effect on glioblastoma cell lines and effectively opened the BBB for targeted treatment of glioblastoma in a mouse model.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Medicine, Research & Experimental
Frederic Padilla, Jacqueline Brenner, Francesco Prada, Alexander L. Klibanov
Summary: Ultrasound-triggered microbubbles destruction can lead to vascular shutdown and has shown potential in preclinical studies for tumor growth delay, lesion formation, radio-sensitization, and modulation of the immune micro-environment. Antivascular ultrasound has the potential to be a focal, targeted, non-invasive, mechanical, and non-thermal treatment either alone or in combination with other therapies. The main mechanism behind these effects is believed to be cavitation, with both non-inertial and inertial cavitation playing significant roles.
Article
Engineering, Biomedical
Yunfan Pan, Yongjian Li, Yan Li, Xiaobing Zheng, Chenghong Zou, Jiang Li, Haosheng Chen
Summary: Thrombosis is a critical medical condition that can result in severe consequences. This study proposes a novel approach called sonothrombolysis, which uses nanodroplet-coated microbubbles and a two-step cavitation strategy to accelerate the dissolution of blood clots. The results demonstrate that this method shows promise in improving thrombolytic efficiency and can serve as an effective drug carrier for targeted drug delivery.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Martynas Maciulevicius, Diana Navickaite, Sonam Chopra, Baltramiejus Jakstys, Saulius Satkauskas
Summary: Research has shown that different calcium concentrations affect cell membrane permeability and cell viability during sonoporation, with ultrasound-microbubble cavitation playing a significant role. The study also indicates that inertial cavitation activities are directly involved in calcium delivery via sonoporation, leading to rapid cell death.
Article
Biotechnology & Applied Microbiology
Maliheh Hasannia, Khalil Abnous, Seyed Mohammad Taghdisi, Sirous Nekooei, Mohammad Ramezani, Mona Alibolandi
Summary: In this study, polypeptide-based vesicles called peptosomes were prepared using synthetic amphiphilic polypeptide-based PEG-PBLG diblock copolymer, and were successfully used for co-delivery of doxorubicin and gold nanorods. The results showed that the targeted peptosomes exhibited higher cellular uptake and cytotoxicity, and demonstrated good performance in both therapy and diagnosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Jide He, Zenan Liu, Xuehua Zhu, Haizhui Xia, Huile Gao, Jian Lu
Summary: This article reviews the research progress of ultrasound-mediated microbubble cavitation in the treatment of solid tumors, emphasizing its potential to reverse chemoresistance, improve efficacy sensitization response, and reduce adverse side effects.
Article
Nanoscience & Nanotechnology
Avinoam Bar-Zion, Atousa Nourmahnad, David R. Mittelstein, Shirin Shivaei, Sangjin Yoo, Marjorie T. Buss, Robert C. Hurt, Dina Malounda, Mohamad H. Abedi, Audrey Lee-Gosselin, Margaret B. Swift, David Maresca, Mikhail G. Shapiro
Summary: Recent advancements in molecular engineering and synthetic biology have enabled the development of biomolecular and cell-based therapies with high molecular specificity, but limited spatiotemporal control. Gas vesicles, a genetically encodable air-filled protein nanostructure, can be engineered to deliver potent mechanical effects at specific locations inside the body through ultrasound-induced inertial cavitation. This capability has potential applications in remotely actuated cell-killing and tissue-disrupting agents, demonstrating the therapeutic potential of gas vesicles under focused ultrasound actuation.
NATURE NANOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Oystein Eikrem, Spiros Kotopoulis, Mihaela Popa, Mireia Mayoral Safont, Kjell Ove Fossan, Sabine Leh, Lea Landolt, Janka Babickova, Oddrun Anita Gudbrandsen, Odd Helge Gilja, Bettina Riedel, Jan Schjott, Emmet McCormack, Hans-Peter Marti
Summary: The use of ultrasound and microbubble-enhanced drug delivery, known as sonoporation, has shown promising results in numerous clinical trials. However, further studies are needed to assess the impact on healthy tissues. This study focused on testing the feasibility of low-intensity sonoporation on healthy kidneys and found that it has the potential to enhance drug uptake without causing long-standing damage to the kidneys.
Article
Pharmacology & Pharmacy
Mohammad Varzandeh, Jaleh Varshosaz, Sheyda Labbaf, Nafiseh Esmaeil
Summary: A new remotely controlled drug delivery, radio-sensitizing, and photothermal therapy agent based on thioglycolic acid modified bismuth nanosheets was evaluated. The nanosheets were synthesized using low energy sonication and were 40-60 nm in size and 1-3 atomic layers thick. The surface modification with thioglycolic acid enhanced the drug loading capacity and improved drug release. Under laser irradiation, the modified nanosheets showed high photothermal conversion efficiency. The combined therapy effectively damaged cancer cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Oncology
Biqiang Zhou, Qingshu Lian, Chunchun Jin, Jianghao Lu, Lifeng Xu, Xuehao Gong, Peng Zhou
Summary: This study investigated the use of diagnostic ultrasound combined with microbubbles in HER2-negative breast cancer. The results showed that this combination therapy significantly reduced tumor size and improved pathologic complete response rate without causing additional toxicity or side effects.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Mingjun Xuan, Jilin Fan, Vu Ngoc Khiem, Miancheng Zou, Kai-Oliver Brenske, Ahmed Mourran, Rostislav Vinokur, Lifei Zheng, Mikhail Itskov, Robert Goestl, Andreas Herrmann
Summary: Polymer mechanochemistry is a promising technology to convert mechanical energy into chemical functionality by breaking bonds. In this study, a microbubble system was used to accelerate the mechanochemical activation of disulfides, and optimization conditions were identified.
ADVANCED MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Dui Qin, Lei Zhang, Hongrui Zhu, Junjie Chen, Daocheng Wu, Ayache Bouakaz, Mingxi Wan, Yi Feng
Summary: A highly efficient nanodroplet with multiple capabilities was developed for cancer therapy, showing advantages in high photothermal conversion efficiency, enhanced US imaging, and acoustic cavitation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Pharmacology & Pharmacy
Daiki Omata, Lisa Munakata, Saori Kageyama, Yuno Suzuki, Tamotsu Maruyama, Tadamitsu Shima, Takumi Chikaarashi, Naoya Kajita, Kohji Masuda, Naoto Tsuchiya, Kazuo Maruyama, Ryo Suzuki
Summary: Gene therapy is a promising technology for genetic and intractable diseases, with drug delivery carriers and systems being studied for improved therapeutic effects. The combination of ultrasound and microbubbles has shown potential for efficient and safe gene delivery to targeted tissues, as demonstrated in a study utilizing a 3D probe and lipid-based microbubbles.
JOURNAL OF DRUG TARGETING
(2022)
Article
Materials Science, Biomaterials
Sayaka Deguchi, Masahiro Tsuda, Kaori Kosugi, Ayaka Sakamoto, Natsumi Mimura, Ryosuke Negoro, Emi Sano, Takuro Nobe, Kazuya Maeda, Hiroyuki Kusuhara, Hiroyuki Mizuguchi, Fumiyoshi Yamashita, Yu-suke Torisawa, Kazuo Takayama
Summary: The study evaluated drug absorption to the PDMS device and investigated the drug responsiveness of human hepatocytes cultured in the PDMS device. The absorption rates of different compounds to the PDMS device were measured and found to be correlated with their octanol/water distribution coefficient values. Furthermore, the hepatocyte-chips were used to examine the response to drugs typically used to evaluate hepatic functions.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2021)
Review
Pharmacology & Pharmacy
Daiki Omata, Lisa Munakata, Kazuo Maruyama, Ryo Suzuki
Summary: Enhancing vascular permeability using ultrasound and microbubbles can improve drug transport to targeted tissues, offering potential therapeutic benefits for a variety of diseases. This approach shows promise in various tissues and drugs applications.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2021)
Article
Physiology
Nagomi Kurebayashi, Takashi Murayama, Ryosaku Ota, Junji Suzuki, Kazunori Kanemaru, Takuya Kobayashi, Seiko Ohno, Minoru Horie, Masamitsu Iino, Fumiyoshi Yamashita, Takashi Sakurai
Summary: Type 2 ryanodine receptor (RYR2) is a cardiac Ca2+ release channel that is associated with catecholaminergic polymorphic ventricular tachycardia (CPVT). This study demonstrates that CPVT mutations lower the threshold [Ca-2(+)](ER) for spontaneous Ca-2(+) release by enhancing the [Ca-2(+)](cyt)-dependent activity of RYR2, without modulating the [Ca-2(+)](ER) sensitivity of RYR2.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Article
Cell Biology
Rodi Abdalkader, Ken-ichiro Kamei
Summary: This study introduces a simplified small molecule-based corneal induction method to generate mature corneal epithelial cells from human pluripotent stem cells. RNA-seq analysis shows that the generated cells closely resemble mature corneal epithelial cells and express relevant markers.
Article
Pharmacology & Pharmacy
Chanikarn Chantarasrivong, Ryu Okada, Yuuki Yamane, Xue Yang, Yuriko Higuchi, Miku Konishi, Naoko Komura, Hiromune Ando, Ryuji Yokokawa, Fumiyoshi Yamashita
Summary: Tumor spheroids with a perfusable vascular network were constructed to evaluate drug delivery systems targeting tumor vasculature. The results suggest that 3'-CE sLeX mimic liposomes are a promising carrier for targeting tumor vasculature, and proinflammatory cytokine treatment may be beneficial in pharmacokinetic studies.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Pharmacology & Pharmacy
Mengyang Wang, Masahiro Tsuda, Sayaka Deguchi, Yuriko Higuchi, Kanako So, Yu-suke Torisawa, Kazuo Takayama, Fumiyoshi Yamashita
Summary: Recently, liver-on-a-chip models have gained increasing attention for pharmacokinetics and toxicity screenings. Researchers have found that perfluoropolyether (PFPE) elastomer, a chemically repellent material, could be an alternative to the commonly used polydimethylsiloxane (PDMS) material in microfluidic devices. The PFPE devices showed improved cell performance with increased production of albumin and urea, as well as enhanced metabolic activity of hepatocytes under dynamic flow conditions compared to static conditions. Although PFPE devices still exhibited some sorption, they demonstrated better chemical repellency and may provide better performance in ADMET evaluation than PDMS devices.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Chemistry, Analytical
Ryohei Ueno, Masahiro Kuninori, Takumi Sumi, Ramin Banan Sadeghian, Yuji Takata, Azusa Iguchi, Masahiro Tsuda, Fumiyoshi Yamashita, Kentaro Ichikawa, Ryuji Yokokawa
Summary: Microphysiological systems (MPS) are emerging as a technology for next-generation non-clinical drug screening. These microfluidic devices mimic the physiological functions of human organs and have the potential to reduce animal experiments, improve drug efficacy prediction, and lower drug discovery costs.
Article
Pharmacology & Pharmacy
Shoko Nomura, Erike W. Sukowati, Yuko Shigeno, Maiko Takahashi, Akari Kato, Yoshimi Benno, Fumiyoshi Yamashita, Hidefumi Mukai
Summary: We demonstrate the potential use of Blautia coccoides JCM1395(T) as a tumor-targeted live bacterial therapeutic. We developed a reliable quantitative analysis method for bacterial detection in biological tissues, overcoming the hindrance caused by the thick outer layer of peptidoglycans in Gram-positive bacteria. Using this method, we individually detected Blautia coccoides JCM1395(T) and Bacteroides vulgatus JCM5826(T) in tumors of mice receiving intravenous mixture injection. This simple and reproducible method, without genetic modification, can be applied to explore a wide range of bacterial species.
Article
Biochemistry & Molecular Biology
Rodi Abdalkader, Romanas Chaleckis, Takuya Fujita
Summary: In this study, a non-targeted metabolomic analysis technique was used to analyze extracellular metabolites in small samples. hiPSCs were differentiated using different combinations of chemical inhibitors and markers were used to determine the differentiation status. Metabolites such as lactic acid and pyruvic acid showed significant changes during differentiation.
Article
Chemistry, Medicinal
Fumiko Hagiwara, Daiki Omata, Lisa Munakata, Saori Kageyama, Kazuo Maruyama, Nobuki Kudo, Ryo Suzuki
Summary: This study evaluated the feasibility of delivering CDDP to the brain for glioblastoma treatment using microbubbles and ultrasound. The results showed that the combination of microbubbles and ultrasound effectively increased the permeability of the blood-brain barrier and enhanced the delivery of CDDP to the glioblastoma site, leading to improved survival in mice.
Review
Ophthalmology
Rodi Kado Abdalkader, Takuya Fujita
Summary: The human corneal epithelial barrier plays a crucial role in drug testing studies, but there is a significant discrepancy between preclinical predictions and clinical outcomes. To tackle this issue, there is a rising demand for alternative in vitro models that closely mimic the human corneal epithelium. Recent advancements in microphysiological systems and stem cell-derived organoids show great promise in developing powerful and biomimetic models of the human cornea.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Ryosaku Ota, Fumiyoshi Yamashita
Summary: This review discusses the current status and challenges of applying machine-learning techniques to the analysis and prediction of pharmacokinetic data. While traditional mathematical models have limitations in explaining ADME processes and predicting pharmacokinetic time profiles, machine learning offers a data-driven approach for handling complex phenomena. Machine-learning models generally outperform linear models and new structures, such as transfer learning and generative adversarial networks, are continuously being proposed. The key issue now is how to apply these emerging machine-learning technologies to the field of pharmacokinetics/pharmacodynamics.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Yuko Sasaki, Hirotaka Tatsuoka, Masahiro Tsuda, Takumi Sumi, Yuka Eguchi, Kanako So, Yuriko Higuchi, Kazuo Takayama, Yusuke Torisawa, Fumiyoshi Yamashita
Summary: Microfluidic devices are a promising tool for studying drug transport and metabolism. This study investigated drug transport across Caco-2 cell layers in microfluidic devices and found that drug lipophilicity influenced permeability. Fluidic conditions also promoted drug metabolism. These findings provide valuable information for the application of microfluidic devices in drug research.
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2022)
Article
Toxicology
Qiyue Zhang, Shiori Taniguchi, Kanako So, Masahiro Tsuda, Yuriko Higuchi, Mitsuru Hashida, Fumiyoshi Yamashita
Summary: Drug-induced liver injury (DILI) is a major cause of drug development failure and post-approval withdrawal. This study identified CREB as a potential sensitive biomarker for DILI prediction and suggested that its response to stress induced by high-risk drugs might be primarily regulated by the PKA/CREB signaling pathway.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2022)
