Article
Biochemical Research Methods
Irina Yu Yanina, Yohei Tanikawa, Elina A. Genina, Polina A. Dyachenko, Daria K. Tuchina, Alexey N. Bashkatov, Leonid E. Dolotov, Yana Tarakanchikova, Georgy S. Terentuk, Nikita A. Navolokin, Alla B. Bucharskaya, Galina N. Maslyakova, Yasunobu Iga, Shinichi Takimoto, Valery V. Tuchin
Summary: This study focuses on the development of technology for optical clearing (OC) of abdominal adipose tissue in vivo using hyperosmotic optical clearing agents (OCAs). The results demonstrate that OCAs can effectively clear adipose tissue and induce cell necrosis to a certain extent, both in ex vivo and in vivo experiments.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Cardiac & Cardiovascular Systems
Alessandro Mengozzi, Sarah Costantino, Francesco Paneni, Emiliano Duranti, Monica Nannipieri, Rudj Mancini, Michele Lai, Veronica La Rocca, Ilaria Puxeddu, Luca Antonioli, Matteo Fornai, Marco Ghionzoli, Georgios Georgiopoulos, Chiara Ippolito, Nunzia Bernardini, Frank Ruschitzka, Nicola Riccardo Pugliese, Stefano Taddei, Agostino Virdis, Stefano Masi
Summary: This study aimed to investigate the role of SIRT1 in microvascular dysfunction caused by age and obesity in humans. The results showed that SIRT1 is a central regulator of early microvascular damage caused by age and obesity. Through the regulation of mitochondrial protein expression and mitochondrial reactive oxygen species levels, it influences nitric oxide availability and the expression of proteins involved in the mitochondria respiratory chain. Therapeutic modulation of SIRT1 can restore obesity- and age-related endothelial dysfunction.
CIRCULATION RESEARCH
(2022)
Article
Ophthalmology
Katelyn K. Dilley, Pamela A. Borden, Yueqiao Qu, Andrew E. Heidari, Karthik R. Prasad, Yan Li, Chung Ho Sun, Zhongping Chen, Sehwan Kim, Michael G. Hill, Brian J. F. Wong
Summary: This study investigated the potential of potential-driven electrochemical treatment (P-ECT) to restore clarity after alkaline-based corneal chemical injuries (CCI). The results showed that P-ECT could restore local optical clarity after NaOH exposure. Analysis of collagen fibril orientation did not show any changes after NaOH exposure or P-ECT. This study suggests that P-ECT may be an effective and low-cost treatment for alkaline CCI.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Ali Jaafar, Roman Holomb, Anton Y. Sdobnov, Zsombor Ocskay, Zoltan Jakus, Valery V. Tuchin, Miklos Veres
Summary: The study investigated the effect of tissue optical clearing (TOC) on ex vivo porcine dura mater using confocal Raman microspectroscopy (CRM) to increase probing depth and observe in-depth structure. It found that the TOC process involves three main steps, including tissue dehydration, glycerol penetration, and collagen dissociation, with glycerol significantly affecting collagen hydration. This study is the first to introduce the TOC technique in assisting CRM of ex vivo dura mater in-depth probing.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Multidisciplinary Sciences
Jia-Hong Lai, En-Yu Liao, Yi-Hua Liao, Chi-Kuang Sun
Summary: The optical clearing of ex vivo human skin by 50% glycerol topical application was investigated in this study. Harmonic generation microscopy was used to examine the clearing effect, showing improved optical homogeneity and reduced scattering in the epidermis layer. The critical role of stratum corneum shrinkage for SHG and THG signal recovery was also demonstrated.
SCIENTIFIC REPORTS
(2021)
Article
Ophthalmology
Wenhan Lu, Zhenle Pei, Wei Hu, Chen Tan, Xiaoyu Tong, Yi Feng, Xinghuai Sun
Summary: Tissue optical clearing is a growing technique that overcomes light scattering in tissues, allowing for detailed structural observations. In recent years, advanced tissue clearing techniques have been optimized for specific tissues and organs, with a focus on applications in eye tissue. The future potential of optical clearing applications in clinical ophthalmology is also being discussed.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemical Research Methods
Sergey M. Zaytsev, Marine Amouroux, Gregoire Khairallah, Alexey N. Bashkatov, Valery V. Tuchin, Walter Blondel, Elina A. Genina
Summary: The study investigated the effect of optical clearing agents on a hybrid model and found an increase in fluorescence reflected intensity, with OCA 2 showing stronger impact possibly due to its effect on the stratum corneum keratin conformation. Additionally, increased light transmittance through the skin was observed, indicating improvement in depth sensitivity of spectroscopy not only due to optical clearing, but also mechanical compression caused by the spectroscopic probe.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Multidisciplinary Sciences
Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Yoshikatsu Sato, Tetsuya Higashiyama
Summary: This method allows for the optical clearing of plant tissues, including cell structures and intracellular fluorescent proteins, using ClearSee (CS) or ClearSeeAlpha (CSA) reagents. Staining using chemical dyes can also be done in combination with CS and CSA, making it a useful tool for studying various aspects of plant biology.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Engineering, Electrical & Electronic
Jiawei Song, Nan Zeng, Hui Ma, Valery V. Tuchin
Summary: This study proposes a non-invasive polarization optical method to evaluate the microstructural changes in tissues during agent diffusion. The method is validated through experiments using skeletal muscle samples and Monte Carlo simulations. It provides a quantitative characterization of tissue changes and offers insights into the optical clearing stages in tissues.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2023)
Article
Chemistry, Analytical
Austin F. Dunn, Megan A. Catterton, Drake D. Dixon, Rebecca R. Pompano
Summary: The study developed an optimized method to detect dynamic and spatially resolved glucose uptake in living tissue using a fluorescent glucose analogue and tissue slice culture. This new approach provides a user-friendly platform for quantifying dynamic metabolic activities in complex tissue microenvironments, with potential applications in various research fields.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
K. V. Berezin, E. V. Grabarchuk, A. M. Lichter, K. N. Dvoretski, V. V. Tuchin
Summary: The results of in vivo immersion optical clearing of human skin under the action of two different optical clearing agents were obtained using optical coherence tomography and molecular modeling methods. The correlations between the efficiency of optical clearing and the energy of intermolecular interaction of the clearing agents with collagen peptide were established.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Endocrinology & Metabolism
Paola Pregel, Elisa Scala, Michela Bullone, Marina Martano, Linda Nozza, Sara Garberoglio, Roberto Garberoglio, Enrico Bollo, Frine Eleonora Scaglione
Summary: The study compared the size of ablation zones induced by RFA systems using internally cooled perfused vs. non-perfused electrodes in swine thyroids. Results showed that perfused electrodes, especially with 7.0% saline solution, produced larger ablation zones compared to non-perfused electrodes. This increase in electrical conductivity of the tissue resulted in more effective ablation of thyroid nodules.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Hongping Deng, Christian J. Konopka, Suma Prabhu, Suresh Sarkar, Natalia Gonzalez Medina, Muhammad Fayyaz, Opeyemi H. Arogundade, Hashni Epa Vidana Gamage, Sayyed Hamed Shahoei, Duncan Nall, Yeoan Youn, Iwona T. Dobrucka, Christopher O. Audu, Amrita Joshi, William J. Melvin, Katherine A. Gallagher, Paul R. Selvin, Erik R. Nelson, Lawrence W. Dobrucki, Kelly S. Swanson, Andrew M. Smith
Summary: This study presents fluorescent and radioisotopic quantum dots (QDs) for multimodal imaging of macrophage cells. These dextran-mimetic QDs exhibit enhanced optical quantification, long-term photostability, and resistance to chemical fixation compared to other probes.
Article
Neurosciences
Danielle A. Jeffrey, Jackson T. Fontaine, Fabrice Dabertrand
Summary: This article introduces an ex vivo method that replicates the dynamics of blood vessels in the brain. By using this method, researchers were able to observe changes in smooth muscle cells and pericytes in the arterial wall without interfering with neuronal tissue. This method is crucial for studying the differences of mural cells based on their location in microvasculature.
Article
Engineering, Biomedical
Francesco Meani, Giuseppe Barbalace, Davide Meroni, Olivia Pagani, Ulrike Perriard, Alberto Pagnamenta, Andrea Aliverti, Emanuele Meroni
Summary: This paper presents a cancer detection procedure using customised electrical impedance spectroscopy (EIS) in breast cancer surgical samples and analyzes its outcomes. A tissue analyser was developed to measure tissue impedance in breast specimens using low-amplitude alternating current along a broad spectrum of frequencies. Experimental measurements on over one hundred excised breast cancer specimens showed significant differences in impedance between neoplastic and non-neoplastic tissues, with good reproducibility. EIS has the potential to discriminate between healthy tissue and cancer, and its further development may allow for real-time evaluation of the transition zone between cancer and normal tissues during surgery.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Editorial Material
Biochemical Research Methods
Luis M. Oliveira, Igor Meglinski, Valery V. Tuchin
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemical Research Methods
Irina N. N. Dolganova, Arsen K. K. Zotov, Larisa P. P. Safonova, Polina V. V. Aleksandrova, Igor V. V. Reshetov, Kirill I. I. Zaytsev, Valery V. V. Tuchin, Vladimir N. N. Kurlov
Summary: This article introduces a sapphire cryoprobe as a potential solution to the problem of tissue freezing monitoring in modern cryosurgery. The probe consists of a sapphire rod manufactured from Al2O3 melt and optical fibers connected to the source and the detector. It enables the detection of tissue optical response for estimating tissue freezing depth. Experimental tests with a gelatin-intralipid tissue phantom demonstrate the feasibility of this concept, with the probe being able to monitor freezing depth up to 10 mm. This highlights the potential of sapphire-based instruments with optical diagnosis in modern cryosurgery.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Spectroscopy
Ana R. Botelho, Hugo F. Silva, Ines S. Martins, Isa C. Carneiro, Sonia D. Carvalho, Rui M. Henrique, Valery V. Tuchin, Luis M. Oliveira
Summary: A fast calculation method was used to obtain the spectral optical properties of human normal and pathological kidney tissues. Both tissues were found to contain melanin and lipofuscin, with the majority of melanin in the pathological kidney converting into lipofuscin.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Multidisciplinary Sciences
Irina Yu. Yanina, Polina A. A. Dyachenko, Arkady S. S. Abdurashitov, Alexander S. S. Shalin, Igor V. V. Minin, Oleg V. V. Minin, Andrey D. D. Bulygin, Denis A. A. Vrazhnov, Yury V. V. Kistenev, Valery V. V. Tuchin
Summary: The optical properties of adipose tissue (AT) at physiological temperatures and in vivo conditions have not been sufficiently studied. AT, composed mainly of packed cells with a spherical shape, exhibits a complex spatial structure of light reflection or transmission. A mathematical model is proposed to describe light propagation through layers of fat cells, and it is found that optical radiation is sharply focused near the shadow surface of cells and can be split by whispering gallery modes depending on the optical thickness of the cell layer. Numerical simulation and experimental studies using optical coherence tomography demonstrate the importance of sharp local focusing in AT for understanding its optical properties under physiological and heating conditions.
SCIENTIFIC REPORTS
(2023)
Editorial Material
Optics
Qingming Luo, Valery V. Tuchin, Lihong Wang
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
(2023)
Article
Medicine, General & Internal
Alexey A. Selifonov, Andrey S. Rykhlov, Valery V. Tuchin
Summary: The method of immersion optical clearing can reduce light scattering in tissues, improving the use of optical technologies by clinicians. This study examined the optical and molecular diffusion properties of cat ovarian tissues in the follicular and luteal phases under the influence of glycerol. Results showed that the reflectance and transmittance of the ovaries were significantly lower in the range of 200 to 600 nm compared to longer wavelengths, and the efficiency of optical clearing was lower in the luteal phase. The diffusion coefficient of glycerol/tissue water was estimated, and the time of complete dehydration of ovarian samples was determined.
Article
Biochemical Research Methods
Sergey. M. M. Zaytsev, Marine Amouroux, Valery. V. V. Tuchin, Elina. A. A. Genina, Walter Blondel
Summary: This study aimed to evaluate the clearing-effectiveness of biocompatible optical clearing agents (OCAs) on human skin, and to enhance skin permeability to OCAs using physical and chemical methods. The results showed that the mixture of polyethylene glycol, oleic acid, and propylene glycol had the best clearing effect, improving the contrast and intensity of skin images. By combining physical and chemical permeation enhancers, such OCAs may improve the diagnostic efficacy of line-field confocal optical coherence tomography (LC-OCT).
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Biochemical Research Methods
Ali Jaafar, Abbas Albarazanchi, Mohammed Jawad Kadhim, Maxim E. Darvin, Tamas Vaczi, Valery V. Tuchin, Miklos Veres
Summary: The influence of e-liquid on porcine lung immersed in it was investigated using confocal Raman micro-spectroscopy. The results showed that the Raman band intensities related to the lung increased after treatment, except for the surface, which was due to the optical clearing effect caused by alveoli filling with e-liquid. Additionally, oxidative stress and collagen dehydration induced by e-liquid were observed. These findings suggest that nicotine-flavour-free e-liquids could potentially be used as optical clearing agents for enhanced Raman-guided bronchoscopy.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemical Research Methods
Yimeng Wang, Da Huang, Kaiqiang Shu, Yingtong Xu, Yixiang Duan, Qingwen Fan, Qingyu Lin, Valery V. Tuchin
Summary: In this study, an innovative method based on laser-induced breakdown spectroscopy (LIBS) combined with machine learning was developed to distinguish and classify different tumor cell lines. Convolutional neural network (CNN) and support vector machine (SVM) algorithms achieved impressive discrimination performances for tumor cell distinguishing, with an accuracy of 97.72%. The results demonstrate the important role of element heterogeneity in tumor cell distinction and suggest the potential application of LIBS technique for fast classification of tumor cells.
JOURNAL OF BIOPHOTONICS
(2023)
Correction
Biochemistry & Molecular Biology
Roman Verkhovskii, Alexey Ermakov, Oleg Grishin, Mikhail A. Makarkin, Ilya Kozhevnikov, Mikhail Makhortov, Anastasiia Kozlova, Samia Salem, Valery Tuchin, Daniil Bratashov
Article
Medicine, General & Internal
Irina Yu. Yanina, Vadim D. Genin, Elina A. Genina, Dmitry A. Mudrak, Nikita A. Navolokin, Alla B. Bucharskaya, Yury V. Kistenev, Valery V. Tuchin
Summary: This study investigated the effects of e-liquid vaping on the morphological and optical properties of lung tissue. The results showed that e-liquid vaping led to optical clearing of lung tissue and caused emphysematous expansion of the alveoli and thickening of the alveolar septa. Spectral analysis also revealed that the hyperosmotic properties of glycerol and propylene glycol in e-liquids can cause dehydration of lung tissue.
Article
Materials Science, Biomaterials
Yulia I. I. Svenskaya, Ekaterina V. V. Lengert, Yana V. V. Tarakanchikova, Albert R. R. Muslimov, Mariia S. S. Saveleva, Elina A. A. Genina, Igor L. L. Radchenko, Liudmila A. A. Stepanova, Andrey V. V. Vasin, Gleb B. B. Sukhorukov, Liudmila M. M. Tsybalova
Summary: Transcutaneous immunization is a promising non-invasive approach for delivering vaccines to antigen presenting cells in the skin. In this study, a novel protocol for transfollicular immunization using porous calcium carbonate carriers and sonophoresis was developed to deliver an inactivated influenza vaccine to perifollicular cells without disrupting the skin's barrier. In vivo monitoring demonstrated the successful transport of vaccine-loaded particles into hair follicles, and the effectiveness of the protocol was confirmed in an animal model through immune response assays. This study highlights the potential of intra-follicular delivery of vaccines as a less invasive alternative to traditional immunization methods.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biochemical Research Methods
Ines S. Martins, Hugo F. Silva, Ekaterina N. Lazareva, Nikita Chernomyrdin, Kirill Zaytsev, Luis M. Oliveira, Valery V. Tuchin
Summary: A critical analysis of methods and results of measurements of the optical properties of tissues in a wide spectral range is presented in this review. The refractive index of biological tissues and liquids is emphasized, as it is crucial for the effective application of optical imaging and diagnostics. The differences in optical parameters between healthy and pathological tissues are discussed, along with the scattering properties of tissues in the terahertz range and the challenges in measuring the optical properties in this range. Additionally, a method for dynamically analyzing the optical properties of tissues under optical clearing is introduced.
BIOMEDICAL OPTICS EXPRESS
(2023)
