期刊
JOURNAL OF BIOMEDICAL OPTICS
卷 19, 期 1, 页码 -出版社
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.JBO.19.1.017006
关键词
surface plasmon resonance; multiplexing; imaging; pentraxins; sepsis
资金
- Regione Lombardia
- European Commission [FP7-HEALTH-2011-ADITEC-280873, FP7-HEALTH-2011-HiPAD-278831]
This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonic mode electric field above the surface has been reconstructed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 10(-6) refractive index units, while the measured lateral resolution is 6.25 mu m within a field of view of several tenths of mm(2), making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
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